UniProtKB - Q9NWB1 (RFOX1_HUMAN)
RNA binding protein fox-1 homolog 1
RBFOX1
Functioni
GO - Molecular functioni
- mRNA binding Source: GO_Central
- protein C-terminus binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- mRNA processing Source: UniProtKB-KW
- nervous system development Source: GO_Central
- regulation of alternative mRNA splicing, via spliceosome Source: GO_Central
- RNA splicing Source: UniProtKB-KW
- RNA transport Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
PathwayCommonsi | Q9NWB1 |
Reactomei | R-HSA-9022707, MECP2 regulates transcription factors |
Names & Taxonomyi
Protein namesi | Recommended name: RNA binding protein fox-1 homolog 1Alternative name(s): Ataxin-2-binding protein 1 Fox-1 homolog A Hexaribonucleotide-binding protein 1 |
Gene namesi | Name:RBFOX1 Synonyms:A2BP, A2BP1, FOX1, HRNBP1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18222, RBFOX1 |
MIMi | 605104, gene |
neXtProti | NX_Q9NWB1 |
VEuPathDBi | HostDB:ENSG00000078328.19 |
Subcellular locationi
Golgi apparatus
- trans-Golgi network Source: UniProtKB
Nucleus
- nuclear stress granule Source: FlyBase
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB
- cytoplasmic stress granule Source: FlyBase
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 120 | H → A: Reduces RNA-binding affinity 160-fold. 1 Publication | 1 | |
Mutagenesisi | 126 | F → A, I or R: Reduces RNA-binding affinity 1500-fold. 1 Publication | 1 | |
Mutagenesisi | 126 | F → H or W: Reduces RNA-binding affinity 15-fold. 1 Publication | 1 | |
Mutagenesisi | 126 | F → Y: No effect on RNA-binding. 1 Publication | 1 | |
Mutagenesisi | 158 | F → A: Reduces RNA-binding affinity 700-fold. 1 Publication | 1 | |
Mutagenesisi | 160 | F → A: Reduces RNA-binding affinity 30'000-fold. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 54715 |
MalaCardsi | RBFOX1 |
OpenTargetsi | ENSG00000078328 |
Miscellaneous databases
Pharosi | Q9NWB1, Tbio |
Genetic variation databases
BioMutai | RBFOX1 |
DMDMi | 166897979 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081480 | 1 – 397 | RNA binding protein fox-1 homolog 1Add BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 317 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 388 | Omega-N-methylarginineBy similarity | 1 |
Keywords - PTMi
MethylationProteomic databases
EPDi | Q9NWB1 |
jPOSTi | Q9NWB1 |
MassIVEi | Q9NWB1 |
MaxQBi | Q9NWB1 |
PaxDbi | Q9NWB1 |
PeptideAtlasi | Q9NWB1 |
PRIDEi | Q9NWB1 |
ProteomicsDBi | 82917 [Q9NWB1-1] 82918 [Q9NWB1-2] 82919 [Q9NWB1-3] 82920 [Q9NWB1-4] 82921 [Q9NWB1-5] |
PTM databases
iPTMneti | Q9NWB1 |
PhosphoSitePlusi | Q9NWB1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000078328, Expressed in putamen and 196 other tissues |
ExpressionAtlasi | Q9NWB1, baseline and differential |
Genevisiblei | Q9NWB1, HS |
Organism-specific databases
HPAi | ENSG00000078328, Group enriched (brain, heart muscle, skeletal muscle) |
Interactioni
Subunit structurei
Binds to the C-terminus of ATXN2.
1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 118 | Interaction with RNA | 1 | |
Sitei | 126 | Interaction with RNA | 1 | |
Sitei | 127 | Interaction with RNA | 1 | |
Sitei | 151 | Interaction with RNA | 1 | |
Sitei | 156 | Interaction with RNA | 1 | |
Sitei | 160 | Interaction with RNA | 1 | |
Sitei | 184 | Interaction with RNA | 1 | |
Sitei | 194 | Interaction with RNA | 1 |
Binary interactionsi
Hide detailsQ9NWB1
Isoform 5 [Q9NWB1-5]
GO - Molecular functioni
- protein C-terminus binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 120107, 76 interactors |
IntActi | Q9NWB1, 78 interactors |
STRINGi | 9606.ENSP00000309117 |
Miscellaneous databases
RNActi | Q9NWB1, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | Q9NWB1 |
SMRi | Q9NWB1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NWB1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 117 – 193 | RRMPROSITE-ProRule annotationAdd BLAST | 77 |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000160685 |
InParanoidi | Q9NWB1 |
OMAi | LVYTSAX |
OrthoDBi | 871288at2759 |
PhylomeDBi | Q9NWB1 |
TreeFami | TF315942 |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025670, Fox-1_C_dom IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR017325, RNA-bd_Fox-1 IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12414, Fox-1_C, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF037932, Ataxin_2_bd_A2BP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 16 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP
60 70 80 90 100
EYTGQTTVPE HTLNLYPPAQ THSEQSPADT SAQTVSGTAT QTDDAAPTDG
110 120 130 140 150
QPQTQPSENT ENKSQPKRLH VSNIPFRFRD PDLRQMFGQF GKILDVEIIF
160 170 180 190 200
NERGSKGFGF VTFENSADAD RAREKLHGTV VEGRKIEVNN ATARVMTNKK
210 220 230 240 250
TVNPYTNGWK LNPVVGAVYS PEFYAGTVLL CQANQEGSSM YSAPSSLVYT
260 270 280 290 300
SAMPGFPYPA ATAAAAYRGA HLRGRGRTVY NTFRAAAPPP PIPAYGGVVY
310 320 330 340 350
QDGFYGADIY GGYAAYRYAQ PTPATAAAYS DSYGRVYAAD PYHHALAPAP
360 370 380 390
TYGVGAMNAF APLTDAKTRS HADDVGLVLS SLQASIYRGG YNRFAPY
The sequence of this isoform differs from the canonical sequence as follows:
1-9: MNCEREQLR → MLASQGVLLHPYGVPMIVPAAPYLPGLIQ
302-310: DGFYGADIY → EPVYGNKLLQ
332-397: SYGRVYAADP...RGGYNRFAPY → RNQFVFVAAD...LQPPPTALVP
Computationally mapped potential isoform sequencesi
There are 16 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7Z1U7 | B7Z1U7_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 425 | Annotation score: | ||
J3KNW3 | J3KNW3_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 402 | Annotation score: | ||
A0A087X2B1 | A0A087X2B1_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 375 | Annotation score: | ||
F8VRS4 | F8VRS4_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 391 | Annotation score: | ||
A0A286YEU2 | A0A286YEU2_HUMAN | RNA-binding protein fox-1 homolog 1 | RBFOX1 | 582 | Annotation score: | ||
F5H0M1 | F5H0M1_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 380 | Annotation score: | ||
F8VZG9 | F8VZG9_HUMAN | RNA-binding protein fox-1 homolog 1 | RBFOX1 | 466 | Annotation score: | ||
A0A6Q8PGE0 | A0A6Q8PGE0_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 A2BP1, hCG_1811870 | 354 | Annotation score: | ||
A0A6Q8PH77 | A0A6Q8PH77_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 374 | Annotation score: | ||
A0A6Q8PFM4 | A0A6Q8PFM4_HUMAN | RNA binding protein fox-1 homolog 1 | RBFOX1 | 415 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 92 | T → A in BAA91472 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030874 | 1 – 9 | MNCEREQLR → MLASQGVLLHPYGVPMIVPA APYLPGLIQ in isoform 2, isoform 4 and isoform 5. 2 Publications | 9 | |
Alternative sequenceiVSP_030875 | 226 – 253 | GTVLL…YTSAM → V in isoform 3. 3 PublicationsAdd BLAST | 28 | |
Alternative sequenceiVSP_030876 | 302 – 310 | DGFYGADIY → EPVYGNKLLQ in isoform 2, isoform 4 and isoform 5. 2 Publications | 9 | |
Alternative sequenceiVSP_030877 | 332 – 397 | SYGRV…RFAPY → RNQFVFVAADEISCNTSAVT DEFMLPTPTTTHLLQPPPTA LVP in isoform 5. 2 PublicationsAdd BLAST | 66 | |
Alternative sequenceiVSP_030878 | 358 – 383 | Missing in isoform 4. 1 PublicationAdd BLAST | 26 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF107203 mRNA Translation: AAF78291.1 Different initiation. AF229057 mRNA Translation: AAL71904.1 AF094849 mRNA Translation: AAL83405.1 AF109106 mRNA Translation: AAL83406.1 AF109120 , AF109107, AF109108, AF109109, AF109110, AF109111, AF109112, AF109113, AF109114, AF109115, AF109116, AF109117, AF109118, AF109119 Genomic DNA Translation: AAL83407.1 AF109120 , AF109107, AF109108, AF109109, AF109110, AF109111, AF109112, AF109113, AF109114, AF109115, AF109116, AF109117, AF109119 Genomic DNA Translation: AAL83408.1 AF109120 , AF109107, AF109108, AF109109, AF109110, AF109111, AF109112, AF109113, AF109114, AF109115, AF109116, AF109117, AF109119 Genomic DNA Translation: AAL83409.1 AF448859 mRNA Translation: AAP41925.1 AK001027 mRNA Translation: BAA91472.1 AC005774 Genomic DNA No translation available. AC006075 Genomic DNA No translation available. AC006112 Genomic DNA No translation available. AC007012 Genomic DNA No translation available. AC007217 Genomic DNA No translation available. AC007222 Genomic DNA No translation available. AC007223 Genomic DNA No translation available. AC009135 Genomic DNA No translation available. AC022206 Genomic DNA No translation available. AC023829 Genomic DNA No translation available. AC027683 Genomic DNA No translation available. AC079410 Genomic DNA No translation available. AC125796 Genomic DNA No translation available. AC131390 Genomic DNA No translation available. BC113691 mRNA Translation: AAI13692.1 |
CCDSi | CCDS10531.1 [Q9NWB1-2] CCDS10532.1 [Q9NWB1-5] CCDS45405.1 [Q9NWB1-4] CCDS55983.1 [Q9NWB1-1] CCDS55984.1 [Q9NWB1-3] |
RefSeqi | NP_001135805.1, NM_001142333.1 [Q9NWB1-3] NP_001135806.1, NM_001142334.1 [Q9NWB1-1] NP_061193.2, NM_018723.3 [Q9NWB1-1] NP_665898.1, NM_145891.2 [Q9NWB1-2] NP_665899.1, NM_145892.2 [Q9NWB1-4] NP_665900.1, NM_145893.2 [Q9NWB1-5] XP_016878822.1, XM_017023333.1 [Q9NWB1-1] XP_016878827.1, XM_017023338.1 [Q9NWB1-3] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF107203 mRNA Translation: AAF78291.1 Different initiation. AF229057 mRNA Translation: AAL71904.1 AF094849 mRNA Translation: AAL83405.1 AF109106 mRNA Translation: AAL83406.1 AF109120 , AF109107, AF109108, AF109109, AF109110, AF109111, AF109112, AF109113, AF109114, AF109115, AF109116, AF109117, AF109118, AF109119 Genomic DNA Translation: AAL83407.1 AF109120 , AF109107, AF109108, AF109109, AF109110, AF109111, AF109112, AF109113, AF109114, AF109115, AF109116, AF109117, AF109119 Genomic DNA Translation: AAL83408.1 AF109120 , AF109107, AF109108, AF109109, AF109110, AF109111, AF109112, AF109113, AF109114, AF109115, AF109116, AF109117, AF109119 Genomic DNA Translation: AAL83409.1 AF448859 mRNA Translation: AAP41925.1 AK001027 mRNA Translation: BAA91472.1 AC005774 Genomic DNA No translation available. AC006075 Genomic DNA No translation available. AC006112 Genomic DNA No translation available. AC007012 Genomic DNA No translation available. AC007217 Genomic DNA No translation available. AC007222 Genomic DNA No translation available. AC007223 Genomic DNA No translation available. AC009135 Genomic DNA No translation available. AC022206 Genomic DNA No translation available. AC023829 Genomic DNA No translation available. AC027683 Genomic DNA No translation available. AC079410 Genomic DNA No translation available. AC125796 Genomic DNA No translation available. AC131390 Genomic DNA No translation available. BC113691 mRNA Translation: AAI13692.1 |
CCDSi | CCDS10531.1 [Q9NWB1-2] CCDS10532.1 [Q9NWB1-5] CCDS45405.1 [Q9NWB1-4] CCDS55983.1 [Q9NWB1-1] CCDS55984.1 [Q9NWB1-3] |
RefSeqi | NP_001135805.1, NM_001142333.1 [Q9NWB1-3] NP_001135806.1, NM_001142334.1 [Q9NWB1-1] NP_061193.2, NM_018723.3 [Q9NWB1-1] NP_665898.1, NM_145891.2 [Q9NWB1-2] NP_665899.1, NM_145892.2 [Q9NWB1-4] NP_665900.1, NM_145893.2 [Q9NWB1-5] XP_016878822.1, XM_017023333.1 [Q9NWB1-1] XP_016878827.1, XM_017023338.1 [Q9NWB1-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2ERR | NMR | - | A | 109-196 | [»] | |
2N82 | NMR | - | B | 109-208 | [»] | |
4ZKA | X-ray | 1.80 | A/B/C/D/E/F | 109-208 | [»] | |
BMRBi | Q9NWB1 | |||||
SMRi | Q9NWB1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120107, 76 interactors |
IntActi | Q9NWB1, 78 interactors |
STRINGi | 9606.ENSP00000309117 |
PTM databases
iPTMneti | Q9NWB1 |
PhosphoSitePlusi | Q9NWB1 |
Genetic variation databases
BioMutai | RBFOX1 |
DMDMi | 166897979 |
Proteomic databases
EPDi | Q9NWB1 |
jPOSTi | Q9NWB1 |
MassIVEi | Q9NWB1 |
MaxQBi | Q9NWB1 |
PaxDbi | Q9NWB1 |
PeptideAtlasi | Q9NWB1 |
PRIDEi | Q9NWB1 |
ProteomicsDBi | 82917 [Q9NWB1-1] 82918 [Q9NWB1-2] 82919 [Q9NWB1-3] 82920 [Q9NWB1-4] 82921 [Q9NWB1-5] |
Protocols and materials databases
Antibodypediai | 24505, 365 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 54715 |
DisGeNETi | 54715 |
GeneCardsi | RBFOX1 |
HGNCi | HGNC:18222, RBFOX1 |
HPAi | ENSG00000078328, Group enriched (brain, heart muscle, skeletal muscle) |
MalaCardsi | RBFOX1 |
MIMi | 605104, gene |
neXtProti | NX_Q9NWB1 |
OpenTargetsi | ENSG00000078328 |
VEuPathDBi | HostDB:ENSG00000078328.19 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0125, Eukaryota |
GeneTreei | ENSGT00940000160685 |
InParanoidi | Q9NWB1 |
OMAi | LVYTSAX |
OrthoDBi | 871288at2759 |
PhylomeDBi | Q9NWB1 |
TreeFami | TF315942 |
Enzyme and pathway databases
PathwayCommonsi | Q9NWB1 |
Reactomei | R-HSA-9022707, MECP2 regulates transcription factors |
Miscellaneous databases
BioGRID-ORCSi | 54715, 3 hits in 875 CRISPR screens |
ChiTaRSi | RBFOX1, human |
EvolutionaryTracei | Q9NWB1 |
GeneWikii | RBFOX1 |
GenomeRNAii | 54715 |
Pharosi | Q9NWB1, Tbio |
PROi | PR:Q9NWB1 |
RNActi | Q9NWB1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000078328, Expressed in putamen and 196 other tissues |
ExpressionAtlasi | Q9NWB1, baseline and differential |
Genevisiblei | Q9NWB1, HS |
Family and domain databases
CDDi | cd12407, RRM_FOX1_like, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR025670, Fox-1_C_dom IPR034237, FOX1_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR017325, RNA-bd_Fox-1 IPR000504, RRM_dom |
Pfami | View protein in Pfam PF12414, Fox-1_C, 1 hit PF00076, RRM_1, 1 hit |
PIRSFi | PIRSF037932, Ataxin_2_bd_A2BP, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RFOX1_HUMAN | |
Accessioni | Q9NWB1Primary (citable) accession number: Q9NWB1 Secondary accession number(s): Q7Z7I7 Q9NS20 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | February 5, 2008 | |
Last modified: | February 10, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references