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Protein

Mediator of RNA polymerase II transcription subunit 9

Gene

MED9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 9
Alternative name(s):
Mediator complex subunit 9
Gene namesi
Name:MED9
Synonyms:MED25
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000141026.5
HGNCiHGNC:25487 MED9
MIMi609878 gene
neXtProtiNX_Q9NWA0

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55090
OpenTargetsiENSG00000141026
PharmGKBiPA134925396

Polymorphism and mutation databases

BioMutaiMED9
DMDMi74734632

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003041472 – 146Mediator of RNA polymerase II transcription subunit 9Add BLAST145

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei110PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NWA0
MaxQBiQ9NWA0
PaxDbiQ9NWA0
PeptideAtlasiQ9NWA0
PRIDEiQ9NWA0
ProteomicsDBi82916

PTM databases

iPTMnetiQ9NWA0
PhosphoSitePlusiQ9NWA0

Expressioni

Gene expression databases

BgeeiENSG00000141026 Expressed in 155 organ(s), highest expression level in heart left ventricle
CleanExiHS_MED25
HS_MED9
ExpressionAtlasiQ9NWA0 baseline and differential
GenevisibleiQ9NWA0 HS

Organism-specific databases

HPAiHPA056415

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.2 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi120403, 52 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9NWA0
IntActiQ9NWA0, 71 interactors
STRINGi9606.ENSP00000268711

Structurei

3D structure databases

ProteinModelPortaliQ9NWA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili112 – 139Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi17 – 54Pro-richAdd BLAST38

Sequence similaritiesi

Belongs to the Mediator complex subunit 9 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410J0JM Eukaryota
ENOG4111U35 LUCA
GeneTreeiENSGT00390000017379
HOGENOMiHOG000113551
HOVERGENiHBG105579
InParanoidiQ9NWA0
KOiK15149
OMAiNVIKCMD
OrthoDBiEOG091G15BA
PhylomeDBiQ9NWA0
TreeFamiTF324926

Family and domain databases

InterProiView protein in InterPro
IPR037212 Med7/Med21-like
IPR011425 Med9
IPR039242 MED9_metazoa
PANTHERiPTHR20844 PTHR20844, 1 hit
PfamiView protein in Pfam
PF07544 Med9, 1 hit
SUPFAMiSSF140718 SSF140718, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

Q9NWA0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASAGVAAGR QAEDVLPPTS DQPLPDTKPL PPPQPPPVPA PQPQQSPAPR
60 70 80 90 100
PQSPARAREE ENYSFLPLVH NIIKCMDKDS PEVHQDLNAL KSKFQEMRKL
110 120 130 140
ISTMPGIHLS PEQQQQQLQS LREQVRTKNE LLQKYKSLCM FEIPKE
Length:146
Mass (Da):16,403
Last modified:October 1, 2000 - v1
Checksum:i24CDB7CBDFD36D1A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
J3KTK5J3KTK5_HUMAN
Mediator complex subunit 9
MED9
82Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001055 mRNA Translation: BAA91483.1
CH471196 Genomic DNA Translation: EAW55706.1
BC104988 mRNA Translation: AAI04989.1
BC112029 mRNA Translation: AAI12030.1
CCDSiCCDS11184.1
RefSeqiNP_060489.1, NM_018019.2
UniGeneiHs.244595

Genome annotation databases

EnsembliENST00000268711; ENSP00000268711; ENSG00000141026
GeneIDi55090
KEGGihsa:55090
UCSCiuc002grh.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001055 mRNA Translation: BAA91483.1
CH471196 Genomic DNA Translation: EAW55706.1
BC104988 mRNA Translation: AAI04989.1
BC112029 mRNA Translation: AAI12030.1
CCDSiCCDS11184.1
RefSeqiNP_060489.1, NM_018019.2
UniGeneiHs.244595

3D structure databases

ProteinModelPortaliQ9NWA0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120403, 52 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9NWA0
IntActiQ9NWA0, 71 interactors
STRINGi9606.ENSP00000268711

PTM databases

iPTMnetiQ9NWA0
PhosphoSitePlusiQ9NWA0

Polymorphism and mutation databases

BioMutaiMED9
DMDMi74734632

Proteomic databases

EPDiQ9NWA0
MaxQBiQ9NWA0
PaxDbiQ9NWA0
PeptideAtlasiQ9NWA0
PRIDEiQ9NWA0
ProteomicsDBi82916

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000268711; ENSP00000268711; ENSG00000141026
GeneIDi55090
KEGGihsa:55090
UCSCiuc002grh.2 human

Organism-specific databases

CTDi55090
DisGeNETi55090
EuPathDBiHostDB:ENSG00000141026.5
GeneCardsiMED9
HGNCiHGNC:25487 MED9
HPAiHPA056415
MIMi609878 gene
neXtProtiNX_Q9NWA0
OpenTargetsiENSG00000141026
PharmGKBiPA134925396
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J0JM Eukaryota
ENOG4111U35 LUCA
GeneTreeiENSGT00390000017379
HOGENOMiHOG000113551
HOVERGENiHBG105579
InParanoidiQ9NWA0
KOiK15149
OMAiNVIKCMD
OrthoDBiEOG091G15BA
PhylomeDBiQ9NWA0
TreeFamiTF324926

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

ChiTaRSiMED9 human
GenomeRNAii55090
PROiPR:Q9NWA0
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000141026 Expressed in 155 organ(s), highest expression level in heart left ventricle
CleanExiHS_MED25
HS_MED9
ExpressionAtlasiQ9NWA0 baseline and differential
GenevisibleiQ9NWA0 HS

Family and domain databases

InterProiView protein in InterPro
IPR037212 Med7/Med21-like
IPR011425 Med9
IPR039242 MED9_metazoa
PANTHERiPTHR20844 PTHR20844, 1 hit
PfamiView protein in Pfam
PF07544 Med9, 1 hit
SUPFAMiSSF140718 SSF140718, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED9_HUMAN
AccessioniPrimary (citable) accession number: Q9NWA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: October 1, 2000
Last modified: September 12, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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