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Protein

Adaptin ear-binding coat-associated protein 2

Gene

NECAP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in endocytosis.By similarity

GO - Biological processi

Keywordsi

Biological processEndocytosis, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Adaptin ear-binding coat-associated protein 2
Alternative name(s):
NECAP endocytosis-associated protein 2
Short name:
NECAP-2
Gene namesi
Name:NECAP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000157191.19
HGNCiHGNC:25528 NECAP2
MIMi611624 gene
neXtProtiNX_Q9NVZ3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000157191
PharmGKBiPA142671268

Polymorphism and mutation databases

BioMutaiNECAP2
DMDMi62287168

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002130711 – 263Adaptin ear-binding coat-associated protein 2Add BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei181PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NVZ3
MaxQBiQ9NVZ3
PaxDbiQ9NVZ3
PeptideAtlasiQ9NVZ3
PRIDEiQ9NVZ3
ProteomicsDBi82880
82881 [Q9NVZ3-2]
82882 [Q9NVZ3-3]
82883 [Q9NVZ3-4]

PTM databases

iPTMnetiQ9NVZ3
PhosphoSitePlusiQ9NVZ3

Expressioni

Gene expression databases

BgeeiENSG00000157191 Expressed in 217 organ(s), highest expression level in oocyte
CleanExiHS_NECAP2
ExpressionAtlasiQ9NVZ3 baseline and differential
GenevisibleiQ9NVZ3 HS

Organism-specific databases

HPAiHPA028077

Interactioni

Subunit structurei

Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).By similarity

Protein-protein interaction databases

BioGridi120832, 32 interactors
IntActiQ9NVZ3, 10 interactors
STRINGi9606.ENSP00000391942

Structurei

3D structure databases

ProteinModelPortaliQ9NVZ3
SMRiQ9NVZ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi240 – 243WXXF motif 14
Motifi260 – 263WXXF motif 24

Domaini

The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 through hydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned by the acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity).By similarity

Sequence similaritiesi

Belongs to the NECAP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2500 Eukaryota
ENOG41113PS LUCA
GeneTreeiENSGT00390000009359
HOGENOMiHOG000231188
HOVERGENiHBG060621
InParanoidiQ9NVZ3
KOiK20069
OMAiVSWPQPK
OrthoDBiEOG091G0PZV
PhylomeDBiQ9NVZ3
TreeFamiTF314482

Family and domain databases

CDDicd13228 PHear_NECAP, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR012466 NECAP_PHear
IPR011993 PH-like_dom_sf
PfamiView protein in Pfam
PF07933 DUF1681, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NVZ3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA
60 70 80 90 100
KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG
110 120 130 140 150
RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL
160 170 180 190 200
GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI
210 220 230 240 250
PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST
260
SSQTQPGTGW VQF
Length:263
Mass (Da):28,339
Last modified:October 1, 2000 - v1
Checksum:i6F4DE1B8335852D4
GO
Isoform 2 (identifier: Q9NVZ3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-263: GGAPVPWPQP...TQPGTGWVQF → DQLPARPSQA...ALLSWPVFGA

Show »
Length:273
Mass (Da):29,464
Checksum:i463D31DEFCA0102E
GO
Isoform 3 (identifier: Q9NVZ3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     164-172: NMKKKEGAA → VSSTLAWLW
     173-263: Missing.

Note: No experimental confirmation available.
Show »
Length:172
Mass (Da):19,419
Checksum:i0EBC5856B326788A
GO
Isoform 4 (identifier: Q9NVZ3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     6-31: Missing.

Show »
Length:237
Mass (Da):25,267
Checksum:i8F5B5B501EEE8E8E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RAN6D6RAN6_HUMAN
Adaptin ear-binding coat-associated...
NECAP2
101Annotation score:
F6SKB8F6SKB8_HUMAN
Adaptin ear-binding coat-associated...
NECAP2
194Annotation score:
H0Y970H0Y970_HUMAN
Adaptin ear-binding coat-associated...
NECAP2
63Annotation score:
D6RB24D6RB24_HUMAN
Adaptin ear-binding coat-associated...
NECAP2
202Annotation score:

Sequence cautioni

The sequence BAB15758 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192P → S in BAG63581 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034154149D → A. Corresponds to variant dbSNP:rs35056694Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417266 – 31Missing in isoform 4. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_013234164 – 172NMKKKEGAA → VSSTLAWLW in isoform 3. 1 Publication9
Alternative sequenceiVSP_013235173 – 263Missing in isoform 3. 1 PublicationAdd BLAST91
Alternative sequenceiVSP_013236223 – 263GGAPV…GWVQF → DQLPARPSQAQAGSSSDLST VFPHVTSGKALPHLGQRKED EALLSWPVFGA in isoform 2. 1 PublicationAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024468 mRNA Translation: BAB15758.1 Different initiation.
AK302227 mRNA Translation: BAG63581.1
AK001282 mRNA Translation: BAA91598.1
AK023545 mRNA Translation: BAB14605.1
AL137802 Genomic DNA No translation available.
AL669962 Genomic DNA No translation available.
BC017014 mRNA Translation: AAH17014.1
BC018914 mRNA Translation: AAH18914.1
CCDSiCCDS173.1 [Q9NVZ3-1]
CCDS44066.1 [Q9NVZ3-2]
CCDS44067.1 [Q9NVZ3-4]
RefSeqiNP_001138749.1, NM_001145277.1 [Q9NVZ3-2]
NP_001138750.1, NM_001145278.1 [Q9NVZ3-4]
NP_060560.1, NM_018090.4 [Q9NVZ3-1]
UniGeneiHs.437385

Genome annotation databases

EnsembliENST00000337132; ENSP00000338746; ENSG00000157191 [Q9NVZ3-1]
ENST00000443980; ENSP00000391942; ENSG00000157191 [Q9NVZ3-2]
ENST00000457722; ENSP00000407091; ENSG00000157191 [Q9NVZ3-4]
ENST00000492095; ENSP00000427620; ENSG00000157191 [Q9NVZ3-1]
GeneIDi55707
KEGGihsa:55707
UCSCiuc001ayo.4 human [Q9NVZ3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024468 mRNA Translation: BAB15758.1 Different initiation.
AK302227 mRNA Translation: BAG63581.1
AK001282 mRNA Translation: BAA91598.1
AK023545 mRNA Translation: BAB14605.1
AL137802 Genomic DNA No translation available.
AL669962 Genomic DNA No translation available.
BC017014 mRNA Translation: AAH17014.1
BC018914 mRNA Translation: AAH18914.1
CCDSiCCDS173.1 [Q9NVZ3-1]
CCDS44066.1 [Q9NVZ3-2]
CCDS44067.1 [Q9NVZ3-4]
RefSeqiNP_001138749.1, NM_001145277.1 [Q9NVZ3-2]
NP_001138750.1, NM_001145278.1 [Q9NVZ3-4]
NP_060560.1, NM_018090.4 [Q9NVZ3-1]
UniGeneiHs.437385

3D structure databases

ProteinModelPortaliQ9NVZ3
SMRiQ9NVZ3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120832, 32 interactors
IntActiQ9NVZ3, 10 interactors
STRINGi9606.ENSP00000391942

PTM databases

iPTMnetiQ9NVZ3
PhosphoSitePlusiQ9NVZ3

Polymorphism and mutation databases

BioMutaiNECAP2
DMDMi62287168

Proteomic databases

EPDiQ9NVZ3
MaxQBiQ9NVZ3
PaxDbiQ9NVZ3
PeptideAtlasiQ9NVZ3
PRIDEiQ9NVZ3
ProteomicsDBi82880
82881 [Q9NVZ3-2]
82882 [Q9NVZ3-3]
82883 [Q9NVZ3-4]

Protocols and materials databases

DNASUi55707
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000337132; ENSP00000338746; ENSG00000157191 [Q9NVZ3-1]
ENST00000443980; ENSP00000391942; ENSG00000157191 [Q9NVZ3-2]
ENST00000457722; ENSP00000407091; ENSG00000157191 [Q9NVZ3-4]
ENST00000492095; ENSP00000427620; ENSG00000157191 [Q9NVZ3-1]
GeneIDi55707
KEGGihsa:55707
UCSCiuc001ayo.4 human [Q9NVZ3-1]

Organism-specific databases

CTDi55707
EuPathDBiHostDB:ENSG00000157191.19
GeneCardsiNECAP2
HGNCiHGNC:25528 NECAP2
HPAiHPA028077
MIMi611624 gene
neXtProtiNX_Q9NVZ3
OpenTargetsiENSG00000157191
PharmGKBiPA142671268
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2500 Eukaryota
ENOG41113PS LUCA
GeneTreeiENSGT00390000009359
HOGENOMiHOG000231188
HOVERGENiHBG060621
InParanoidiQ9NVZ3
KOiK20069
OMAiVSWPQPK
OrthoDBiEOG091G0PZV
PhylomeDBiQ9NVZ3
TreeFamiTF314482

Enzyme and pathway databases

ReactomeiR-HSA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-HSA-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiNECAP2 human
GeneWikiiNECAP2
GenomeRNAii55707
PROiPR:Q9NVZ3
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000157191 Expressed in 217 organ(s), highest expression level in oocyte
CleanExiHS_NECAP2
ExpressionAtlasiQ9NVZ3 baseline and differential
GenevisibleiQ9NVZ3 HS

Family and domain databases

CDDicd13228 PHear_NECAP, 1 hit
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR012466 NECAP_PHear
IPR011993 PH-like_dom_sf
PfamiView protein in Pfam
PF07933 DUF1681, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNECP2_HUMAN
AccessioniPrimary (citable) accession number: Q9NVZ3
Secondary accession number(s): B4DY19
, E9PGQ8, Q5VSU4, Q5VSU5, Q9H7L1, Q9H8L1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: October 1, 2000
Last modified: October 10, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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