Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Notchless protein homolog 1

Gene

NLE1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processNotch signaling pathway

Enzyme and pathway databases

SignaLinkiQ9NVX2

Names & Taxonomyi

Protein namesi
Recommended name:
Notchless protein homolog 1
Gene namesi
Name:NLE1
ORF Names:HUSSY-07
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000073536.17
HGNCiHGNC:19889 NLE1
neXtProtiNX_Q9NVX2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi54475
OpenTargetsiENSG00000073536
PharmGKBiPA142671263

Polymorphism and mutation databases

BioMutaiNLE1
DMDMi296439488

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000510982 – 485Notchless protein homolog 1Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei79PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NVX2
MaxQBiQ9NVX2
PaxDbiQ9NVX2
PeptideAtlasiQ9NVX2
PRIDEiQ9NVX2
ProteomicsDBi82876
82877 [Q9NVX2-2]

2D gel databases

SWISS-2DPAGEiQ9NVX2

PTM databases

iPTMnetiQ9NVX2
PhosphoSitePlusiQ9NVX2

Expressioni

Gene expression databases

BgeeiENSG00000073536 Expressed in 123 organ(s), highest expression level in body of pancreas
CleanExiHS_NLE1
ExpressionAtlasiQ9NVX2 baseline and differential
GenevisibleiQ9NVX2 HS

Organism-specific databases

HPAiHPA018807

Interactioni

Subunit structurei

Associates with the pre-60S ribosomal particle. Interacts (via WD repeats) with uL18 (By similarity). Interacts (via UBL domain) with MDN1 (via VWFA/MIDAS domain) (PubMed:26601951).By similarity1 Publication

Protein-protein interaction databases

BioGridi119980, 39 interactors
IntActiQ9NVX2, 6 interactors
MINTiQ9NVX2
STRINGi9606.ENSP00000413572

Structurei

3D structure databases

ProteinModelPortaliQ9NVX2
SMRiQ9NVX2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati112 – 151WD 1Sequence analysisAdd BLAST40
Repeati154 – 193WD 2Sequence analysisAdd BLAST40
Repeati197 – 241WD 3Sequence analysisAdd BLAST45
Repeati244 – 282WD 4Sequence analysisAdd BLAST39
Repeati325 – 366WD 5Sequence analysisAdd BLAST42
Repeati370 – 409WD 6Sequence analysisAdd BLAST40
Repeati412 – 451WD 7Sequence analysisAdd BLAST40
Repeati454 – 485WD 8Sequence analysisAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 96Ubiquitin-like (UBL) domainBy similarityAdd BLAST83

Sequence similaritiesi

Belongs to the NLE1/RSA4 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0271 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091641
HOVERGENiHBG007602
InParanoidiQ9NVX2
KOiK14855
OMAiLNTETPH
OrthoDBiEOG091G05DP
PhylomeDBiQ9NVX2
TreeFamiTF300668

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR012972 NLE
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF08154 NLE, 1 hit
PF00400 WD40, 7 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 8 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 7 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NVX2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAAVPDEAV ARDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDRLQLVCNA
60 70 80 90 100
LLAQEDPLPL AFFVHDAEIV SSLGKTLESQ AVETEKVLDI IYQPQAIFRV
110 120 130 140 150
RAVTRCTSSL EGHSEAVISV AFSPTGKYLA SGSGDTTVRF WDLSTETPHF
160 170 180 190 200
TCKGHRHWVL SISWSPDGRK LASGCKNGQI LLWDPSTGKQ VGRTLAGHSK
210 220 230 240 250
WITGLSWEPL HANPECRYVA SSSKDGSVRI WDTTAGRCER ILTGHTQSVT
260 270 280 290 300
CLRWGGDGLL YSASQDRTIK VWRAHDGVLC RTLQGHGHWV NTMALSTDYA
310 320 330 340 350
LRTGAFEPAE ASVNPQDLQG SLQELKERAL SRYNLVRGQG PERLVSGSDD
360 370 380 390 400
FTLFLWSPAE DKKPLTRMTG HQALINQVLF SPDSRIVASA SFDKSIKLWD
410 420 430 440 450
GRTGKYLASL RGHVAAVYQI AWSADSRLLV SGSSDSTLKV WDVKAQKLAM
460 470 480
DLPGHADEVY AVDWSPDGQR VASGGKDKCL RIWRR
Length:485
Mass (Da):53,320
Last modified:May 18, 2010 - v4
Checksum:iE2E638B13884BC07
GO
Isoform 2 (identifier: Q9NVX2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-292: Missing.

Show »
Length:193
Mass (Da):21,310
Checksum:i01348EBE25FBF31E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MRH0A0A0A0MRH0_HUMAN
Notchless protein homolog 1
NLE1
443Annotation score:
K7EN33K7EN33_HUMAN
Notchless protein homolog 1
NLE1
304Annotation score:
K7ERN7K7ERN7_HUMAN
Notchless protein homolog 1
NLE1
127Annotation score:
K7EK23K7EK23_HUMAN
Notchless protein homolog 1
NLE1
87Annotation score:

Sequence cautioni

The sequence BAD92348 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321S → F in BAD92348 (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0603276P → ACombined sources3 PublicationsCorresponds to variant dbSNP:rs1471615Ensembl.1
Natural variantiVAR_060328169R → K2 PublicationsCorresponds to variant dbSNP:rs7215209Ensembl.1
Natural variantiVAR_035886319Q → K in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs75635495Ensembl.1
Natural variantiVAR_057616406Y → H. Corresponds to variant dbSNP:rs2306513Ensembl.1
Natural variantiVAR_057617434S → N. Corresponds to variant dbSNP:rs2306512Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0405551 – 292Missing in isoform 2. 1 PublicationAdd BLAST292

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001320 mRNA Translation: BAA91621.1
AB209111 mRNA Translation: BAD92348.1 Different initiation.
AC022916 Genomic DNA No translation available.
BC002884 mRNA Translation: AAH02884.2
BC012075 mRNA Translation: AAH12075.1
AJ005257 mRNA Translation: CAA06444.1
CCDSiCCDS11291.1 [Q9NVX2-1]
CCDS45647.1 [Q9NVX2-2]
RefSeqiNP_001014445.1, NM_001014445.2 [Q9NVX2-2]
NP_060566.2, NM_018096.4 [Q9NVX2-1]
XP_016880266.1, XM_017024777.1 [Q9NVX2-2]
UniGeneiHs.85570

Genome annotation databases

EnsembliENST00000442241; ENSP00000413572; ENSG00000073536 [Q9NVX2-1]
ENST00000586869; ENSP00000466588; ENSG00000073536 [Q9NVX2-2]
GeneIDi54475
KEGGihsa:54475
UCSCiuc002hiy.3 human [Q9NVX2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001320 mRNA Translation: BAA91621.1
AB209111 mRNA Translation: BAD92348.1 Different initiation.
AC022916 Genomic DNA No translation available.
BC002884 mRNA Translation: AAH02884.2
BC012075 mRNA Translation: AAH12075.1
AJ005257 mRNA Translation: CAA06444.1
CCDSiCCDS11291.1 [Q9NVX2-1]
CCDS45647.1 [Q9NVX2-2]
RefSeqiNP_001014445.1, NM_001014445.2 [Q9NVX2-2]
NP_060566.2, NM_018096.4 [Q9NVX2-1]
XP_016880266.1, XM_017024777.1 [Q9NVX2-2]
UniGeneiHs.85570

3D structure databases

ProteinModelPortaliQ9NVX2
SMRiQ9NVX2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119980, 39 interactors
IntActiQ9NVX2, 6 interactors
MINTiQ9NVX2
STRINGi9606.ENSP00000413572

PTM databases

iPTMnetiQ9NVX2
PhosphoSitePlusiQ9NVX2

Polymorphism and mutation databases

BioMutaiNLE1
DMDMi296439488

2D gel databases

SWISS-2DPAGEiQ9NVX2

Proteomic databases

EPDiQ9NVX2
MaxQBiQ9NVX2
PaxDbiQ9NVX2
PeptideAtlasiQ9NVX2
PRIDEiQ9NVX2
ProteomicsDBi82876
82877 [Q9NVX2-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000442241; ENSP00000413572; ENSG00000073536 [Q9NVX2-1]
ENST00000586869; ENSP00000466588; ENSG00000073536 [Q9NVX2-2]
GeneIDi54475
KEGGihsa:54475
UCSCiuc002hiy.3 human [Q9NVX2-1]

Organism-specific databases

CTDi54475
DisGeNETi54475
EuPathDBiHostDB:ENSG00000073536.17
GeneCardsiNLE1
HGNCiHGNC:19889 NLE1
HPAiHPA018807
neXtProtiNX_Q9NVX2
OpenTargetsiENSG00000073536
PharmGKBiPA142671263
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0271 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091641
HOVERGENiHBG007602
InParanoidiQ9NVX2
KOiK14855
OMAiLNTETPH
OrthoDBiEOG091G05DP
PhylomeDBiQ9NVX2
TreeFamiTF300668

Enzyme and pathway databases

SignaLinkiQ9NVX2

Miscellaneous databases

ChiTaRSiNLE1 human
GeneWikiiNLE1
GenomeRNAii54475
PROiPR:Q9NVX2

Gene expression databases

BgeeiENSG00000073536 Expressed in 123 organ(s), highest expression level in body of pancreas
CleanExiHS_NLE1
ExpressionAtlasiQ9NVX2 baseline and differential
GenevisibleiQ9NVX2 HS

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR001632 Gprotein_B
IPR012972 NLE
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF08154 NLE, 1 hit
PF00400 WD40, 7 hits
PRINTSiPR00319 GPROTEINB
PR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 8 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 7 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNLE1_HUMAN
AccessioniPrimary (citable) accession number: Q9NVX2
Secondary accession number(s): O60868, Q59GJ8, Q9BU54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: May 18, 2010
Last modified: November 7, 2018
This is version 161 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again