UniProtKB - Q9NVU0 (RPC5_HUMAN)
Protein
DNA-directed RNA polymerase III subunit RPC5
Gene
POLR3E
Organism
Homo sapiens (Human)
Status
Functioni
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).By similarity2 Publications
GO - Molecular functioni
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- innate immune response Source: UniProtKB-KW
- positive regulation of type I interferon production Source: Reactome
- transcription, DNA-templated Source: InterPro
Keywordsi
Biological process | Antiviral defense, Immunity, Innate immunity, Transcription |
Enzyme and pathway databases
PathwayCommonsi | Q9NVU0 |
Reactomei | R-HSA-1834949, Cytosolic sensors of pathogen-associated DNA R-HSA-73780, RNA Polymerase III Chain Elongation R-HSA-73980, RNA Polymerase III Transcription Termination R-HSA-749476, RNA Polymerase III Abortive And Retractive Initiation R-HSA-76061, RNA Polymerase III Transcription Initiation From Type 1 Promoter R-HSA-76066, RNA Polymerase III Transcription Initiation From Type 2 Promoter R-HSA-76071, RNA Polymerase III Transcription Initiation From Type 3 Promoter |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-directed RNA polymerase III subunit RPC5Short name: RNA polymerase III subunit C5 Alternative name(s): DNA-directed RNA polymerase III 80 kDa polypeptide |
Gene namesi | Name:POLR3E Synonyms:KIAA1452 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:30347, POLR3E |
MIMi | 617815, gene |
neXtProti | NX_Q9NVU0 |
VEuPathDBi | HostDB:ENSG00000058600.15 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: HPA
- RNA polymerase III complex Source: MGI
Keywords - Cellular componenti
DNA-directed RNA polymerase, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55718 |
OpenTargetsi | ENSG00000058600 |
PharmGKBi | PA134964025 |
Miscellaneous databases
Pharosi | Q9NVU0, Tbio |
Genetic variation databases
BioMutai | POLR3E |
DMDMi | 29428028 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000073970 | 1 – 708 | DNA-directed RNA polymerase III subunit RPC5Add BLAST | 708 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 161 | PhosphoserineCombined sources | 1 | |
Modified residuei | 162 | PhosphoserineCombined sources | 1 | |
Cross-linki | 171 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 192 | PhosphoserineCombined sources | 1 | |
Modified residuei | 224 | PhosphotyrosineCombined sources | 1 | |
Cross-linki | 432 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 498 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources | ||
Cross-linki | 498 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 503 | PhosphoserineCombined sources | 1 | |
Modified residuei | 522 | PhosphoserineCombined sources | 1 | |
Cross-linki | 659 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9NVU0 |
jPOSTi | Q9NVU0 |
MassIVEi | Q9NVU0 |
MaxQBi | Q9NVU0 |
PaxDbi | Q9NVU0 |
PeptideAtlasi | Q9NVU0 |
PRIDEi | Q9NVU0 |
ProteomicsDBi | 42503 4504 82857 [Q9NVU0-1] 82858 [Q9NVU0-2] 82859 [Q9NVU0-3] |
PTM databases
iPTMneti | Q9NVU0 |
PhosphoSitePlusi | Q9NVU0 |
Expressioni
Gene expression databases
Bgeei | ENSG00000058600, Expressed in left lobe of thyroid gland and 237 other tissues |
ExpressionAtlasi | Q9NVU0, baseline and differential |
Genevisiblei | Q9NVU0, HS |
Organism-specific databases
HPAi | ENSG00000058600, Low tissue specificity |
Interactioni
Subunit structurei
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity).
Interacts with POLR3D/RPC4.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 120840, 57 interactors 3190820, 9 interactors |
IntActi | Q9NVU0, 53 interactors |
MINTi | Q9NVU0 |
STRINGi | 9606.ENSP00000299853 |
Miscellaneous databases
RNActi | Q9NVU0, protein |
Family & Domainsi
Phylogenomic databases
eggNOGi | KOG2354, Eukaryota |
GeneTreei | ENSGT00390000016123 |
HOGENOMi | CLU_021012_1_0_1 |
InParanoidi | Q9NVU0 |
OMAi | MSDQDCC |
OrthoDBi | 1110776at2759 |
PhylomeDBi | Q9NVU0 |
TreeFami | TF103050 |
Family and domain databases
InterProi | View protein in InterPro IPR006886, RNA_pol_III_Rpc5 |
PANTHERi | PTHR12069, PTHR12069, 1 hit |
Pfami | View protein in Pfam PF04801, Sin_N, 1 hit |
s (5+)i Sequence
Sequence statusi: Complete.
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 5 described isoforms and 8 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NVU0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MANEEDDPVV QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK
60 70 80 90 100
PKQQKVELEM AIDTLNPNYC RSKGEQIALN VDGACADETS TYSSKLMDKQ
110 120 130 140 150
TFCSSQTTSN TSRYAAALYR QGELHLTPLH GILQLRPSFS YLDKADAKHR
160 170 180 190 200
EREAANEAGD SSQDEAEDDV KQITVRFSRP ESEQARQRRV QSYEFLQKKH
210 220 230 240 250
AEEPWVHLHY YGLRDSRSEH ERQYLLCPGS SGVENTELVK SPSEYLMMLM
260 270 280 290 300
PPSQEEEKDK PVAPSNVLSM AQLRTLPLAD QIKILMKNVK VMPFANLMSL
310 320 330 340 350
LGPSIDSVAV LRGIQKVAML VQGNWVVKSD ILYPKDSSSP HSGVPAEVLC
360 370 380 390 400
RGRDFVMWKF TQSRWVVRKE VATVTKLCAE DVKDFLEHMA VVRINKGWEF
410 420 430 440 450
ILPYDGEFIK KHPDVVQRQH MLWTGIQAKL EKVYNLVKET MPKKPDAQSG
460 470 480 490 500
PAGLVCGDQR IQVAKTKAQQ NHALLERELQ RRKEQLRVPA VPPGVRIKEE
510 520 530 540 550
PVSEEGEEDE EQEAEEEPMD TSPSGLHSKL ANGLPLGRAA GTDSFNGHPP
560 570 580 590 600
QGCASTPVAR ELKAFVEATF QRQFVLTLSE LKRLFNLHLA SLPPGHTLFS
610 620 630 640 650
GISDRMLQDT VLAAGCKQIL VPFPPQTAAS PDEQKVFALW ESGDMSDQHR
660 670 680 690 700
QVLLEIFSKN YRVRRNMIQS RLTQECGEDL SKQEVDKVLK DCCVSYGGMW
YLKGTVQS
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0C4DH01 | A0A0C4DH01_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 672 | Annotation score: | ||
H3BMR4 | H3BMR4_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 139 | Annotation score: | ||
H3BRY1 | H3BRY1_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 99 | Annotation score: | ||
H3BNJ0 | H3BNJ0_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 90 | Annotation score: | ||
H3BSI5 | H3BSI5_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 110 | Annotation score: | ||
H3BM46 | H3BM46_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 63 | Annotation score: | ||
H3BS41 | H3BS41_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 92 | Annotation score: | ||
H3BSM3 | H3BSM3_HUMAN | DNA-directed RNA polymerase III sub... | POLR3E | 62 | Annotation score: |
Sequence cautioni
The sequence BAA95976 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14481 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 14 | D → N in BAB14437 (PubMed:10819331).Curated | 1 | |
Sequence conflicti | 80 | N → D in BAG62408 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 148 | K → E in BAB14437 (PubMed:10819331).Curated | 1 | |
Sequence conflicti | 316 | K → Q in AAM18215 (PubMed:12391170).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_024623 | 46 | S → A. Corresponds to variant dbSNP:rs2347Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046374 | 13 – 48 | Missing in isoform 5. 1 PublicationAdd BLAST | 36 | |
Alternative sequenceiVSP_007064 | 623 – 708 | FPPQT…GTVQS → VSRALPARGMGGGGWGEPAV GRPRGDTGGHAW in isoform 3. 1 PublicationAdd BLAST | 86 | |
Alternative sequenceiVSP_044710 | 648 – 668 | Missing in isoform 4. 1 PublicationAdd BLAST | 21 | |
Alternative sequenceiVSP_007065 | 649 – 690 | Missing in isoform 2. 2 PublicationsAdd BLAST | 42 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY092085 mRNA Translation: AAM18215.1 AB040885 mRNA Translation: BAA95976.1 Different initiation. AK001371 mRNA Translation: BAA91655.1 AK023160 mRNA Translation: BAB14437.1 AK023231 mRNA Translation: BAB14481.1 Different initiation. AK296813 mRNA Translation: BAG59386.1 AK300735 mRNA Translation: BAG62408.1 AC009034 Genomic DNA No translation available. AC092338 Genomic DNA No translation available. BC000285 mRNA Translation: AAH00285.1 |
CCDSi | CCDS10605.1 [Q9NVU0-1] CCDS58432.1 [Q9NVU0-4] CCDS58433.1 [Q9NVU0-2] CCDS58434.1 [Q9NVU0-5] |
RefSeqi | NP_001244962.1, NM_001258033.1 [Q9NVU0-4] NP_001244963.1, NM_001258034.1 [Q9NVU0-5] NP_001244964.1, NM_001258035.1 NP_001244965.1, NM_001258036.1 [Q9NVU0-2] NP_060589.1, NM_018119.3 [Q9NVU0-1] XP_006721126.1, XM_006721063.2 XP_006726676.1, XM_006726613.1 |
Genome annotation databases
Ensembli | ENST00000299853; ENSP00000299853; ENSG00000058600 [Q9NVU0-1] ENST00000359210; ENSP00000352140; ENSG00000058600 [Q9NVU0-2] ENST00000418581; ENSP00000399254; ENSG00000058600 [Q9NVU0-5] ENST00000564209; ENSP00000456967; ENSG00000058600 [Q9NVU0-4] ENST00000639550; ENSP00000492514; ENSG00000284282 [Q9NVU0-1] ENST00000640588; ENSP00000492263; ENSG00000284282 [Q9NVU0-1] |
GeneIDi | 55718 |
KEGGi | hsa:55718 |
UCSCi | uc002dkk.5, human [Q9NVU0-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY092085 mRNA Translation: AAM18215.1 AB040885 mRNA Translation: BAA95976.1 Different initiation. AK001371 mRNA Translation: BAA91655.1 AK023160 mRNA Translation: BAB14437.1 AK023231 mRNA Translation: BAB14481.1 Different initiation. AK296813 mRNA Translation: BAG59386.1 AK300735 mRNA Translation: BAG62408.1 AC009034 Genomic DNA No translation available. AC092338 Genomic DNA No translation available. BC000285 mRNA Translation: AAH00285.1 |
CCDSi | CCDS10605.1 [Q9NVU0-1] CCDS58432.1 [Q9NVU0-4] CCDS58433.1 [Q9NVU0-2] CCDS58434.1 [Q9NVU0-5] |
RefSeqi | NP_001244962.1, NM_001258033.1 [Q9NVU0-4] NP_001244963.1, NM_001258034.1 [Q9NVU0-5] NP_001244964.1, NM_001258035.1 NP_001244965.1, NM_001258036.1 [Q9NVU0-2] NP_060589.1, NM_018119.3 [Q9NVU0-1] XP_006721126.1, XM_006721063.2 XP_006726676.1, XM_006726613.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120840, 57 interactors 3190820, 9 interactors |
IntActi | Q9NVU0, 53 interactors |
MINTi | Q9NVU0 |
STRINGi | 9606.ENSP00000299853 |
PTM databases
iPTMneti | Q9NVU0 |
PhosphoSitePlusi | Q9NVU0 |
Genetic variation databases
BioMutai | POLR3E |
DMDMi | 29428028 |
Proteomic databases
EPDi | Q9NVU0 |
jPOSTi | Q9NVU0 |
MassIVEi | Q9NVU0 |
MaxQBi | Q9NVU0 |
PaxDbi | Q9NVU0 |
PeptideAtlasi | Q9NVU0 |
PRIDEi | Q9NVU0 |
ProteomicsDBi | 42503 4504 82857 [Q9NVU0-1] 82858 [Q9NVU0-2] 82859 [Q9NVU0-3] |
Protocols and materials databases
Antibodypediai | 25851, 114 antibodies |
Genome annotation databases
Ensembli | ENST00000299853; ENSP00000299853; ENSG00000058600 [Q9NVU0-1] ENST00000359210; ENSP00000352140; ENSG00000058600 [Q9NVU0-2] ENST00000418581; ENSP00000399254; ENSG00000058600 [Q9NVU0-5] ENST00000564209; ENSP00000456967; ENSG00000058600 [Q9NVU0-4] ENST00000639550; ENSP00000492514; ENSG00000284282 [Q9NVU0-1] ENST00000640588; ENSP00000492263; ENSG00000284282 [Q9NVU0-1] |
GeneIDi | 55718 |
KEGGi | hsa:55718 |
UCSCi | uc002dkk.5, human [Q9NVU0-1] |
Organism-specific databases
CTDi | 55718 |
DisGeNETi | 55718 |
GeneCardsi | POLR3E |
HGNCi | HGNC:30347, POLR3E |
HPAi | ENSG00000058600, Low tissue specificity |
MIMi | 617815, gene |
neXtProti | NX_Q9NVU0 |
OpenTargetsi | ENSG00000058600 |
PharmGKBi | PA134964025 |
VEuPathDBi | HostDB:ENSG00000058600.15 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2354, Eukaryota |
GeneTreei | ENSGT00390000016123 |
HOGENOMi | CLU_021012_1_0_1 |
InParanoidi | Q9NVU0 |
OMAi | MSDQDCC |
OrthoDBi | 1110776at2759 |
PhylomeDBi | Q9NVU0 |
TreeFami | TF103050 |
Enzyme and pathway databases
PathwayCommonsi | Q9NVU0 |
Reactomei | R-HSA-1834949, Cytosolic sensors of pathogen-associated DNA R-HSA-73780, RNA Polymerase III Chain Elongation R-HSA-73980, RNA Polymerase III Transcription Termination R-HSA-749476, RNA Polymerase III Abortive And Retractive Initiation R-HSA-76061, RNA Polymerase III Transcription Initiation From Type 1 Promoter R-HSA-76066, RNA Polymerase III Transcription Initiation From Type 2 Promoter R-HSA-76071, RNA Polymerase III Transcription Initiation From Type 3 Promoter |
Miscellaneous databases
BioGRID-ORCSi | 55718, 679 hits in 880 CRISPR screens |
ChiTaRSi | POLR3E, human |
GeneWikii | POLR3E |
Pharosi | Q9NVU0, Tbio |
PROi | PR:Q9NVU0 |
RNActi | Q9NVU0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000058600, Expressed in left lobe of thyroid gland and 237 other tissues |
ExpressionAtlasi | Q9NVU0, baseline and differential |
Genevisiblei | Q9NVU0, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006886, RNA_pol_III_Rpc5 |
PANTHERi | PTHR12069, PTHR12069, 1 hit |
Pfami | View protein in Pfam PF04801, Sin_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPC5_HUMAN | |
Accessioni | Q9NVU0Primary (citable) accession number: Q9NVU0 Secondary accession number(s): B4DL24 Q9P276 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 28, 2003 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot