UniProtKB - Q9NVP2 (ASF1B_HUMAN)
Protein
Histone chaperone ASF1B
Gene
ASF1B
Organism
Homo sapiens (Human)
Status
Functioni
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.5 Publications
GO - Molecular functioni
- histone binding Source: GO_Central
GO - Biological processi
- blastocyst hatching Source: Ensembl
- cell differentiation Source: UniProtKB-KW
- DNA replication-dependent nucleosome assembly Source: UniProtKB
- DNA replication-independent nucleosome assembly Source: UniProtKB
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Molecular function | Chaperone, Chromatin regulator, Developmental protein |
Biological process | Differentiation, Spermatogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | Q9NVP2 |
SIGNORi | Q9NVP2 |
Names & Taxonomyi
Protein namesi | Recommended name: Histone chaperone ASF1BAlternative name(s): Anti-silencing function protein 1 homolog B Short name: hAsf1 Short name: hAsf1b CCG1-interacting factor A-II Short name: CIA-II Short name: hCIA-II |
Gene namesi | Name:ASF1B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:20996, ASF1B |
MIMi | 609190, gene |
neXtProti | NX_Q9NVP2 |
VEuPathDBi | HostDB:ENSG00000105011.8 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: HPA
Other locations
- chromatin Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 36 | D → A: Abolishes CDAN1 interaction. 1 Publication | 1 | |
Mutagenesisi | 37 | D → A: Abolishes CDAN1 interaction. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55723 |
OpenTargetsi | ENSG00000105011 |
PharmGKBi | PA134931112 |
Miscellaneous databases
Pharosi | Q9NVP2, Tbio |
Genetic variation databases
BioMutai | ASF1B |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000284015 | 1 – 202 | Histone chaperone ASF1BAdd BLAST | 202 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 198 | Phosphoserine; by TLK21 Publication | 1 |
Post-translational modificationi
Phosphorylated by TLK1 and TLK2.2 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | CPTAC-953 |
EPDi | Q9NVP2 |
jPOSTi | Q9NVP2 |
MassIVEi | Q9NVP2 |
MaxQBi | Q9NVP2 |
PaxDbi | Q9NVP2 |
PeptideAtlasi | Q9NVP2 |
PRIDEi | Q9NVP2 |
ProteomicsDBi | 82838 |
PTM databases
iPTMneti | Q9NVP2 |
MetOSitei | Q9NVP2 |
PhosphoSitePlusi | Q9NVP2 |
SwissPalmi | Q9NVP2 |
Expressioni
Tissue specificityi
Highly expressed in testis and at lower levels in colon, small intestine and thymus.1 Publication
Gene expression databases
Bgeei | ENSG00000105011, Expressed in oocyte and 132 other tissues |
ExpressionAtlasi | Q9NVP2, baseline and differential |
Genevisiblei | Q9NVP2, HS |
Organism-specific databases
HPAi | ENSG00000105011, Group enriched (blood, lymphoid tissue, testis) |
Interactioni
Subunit structurei
Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4.
Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex.
Interacts with HAT1, NASP, TAF1, TLK1 and TLK2.
Interacts with CDAN1.
Found in a cytosolic complex with CDAN1, ASF1A, IPO4 and histones H3.1 and H4.
Interacts with CREBBP.
9 PublicationsBinary interactionsi
Hide detailsQ9NVP2
With | #Exp. | IntAct |
---|---|---|
CHAF1B [Q13112] | 3 | EBI-1055650,EBI-1052944 |
H3-3B [P84243] | 4 | EBI-1055650,EBI-120658 |
HIRA [P54198] | 4 | EBI-1055650,EBI-372342 |
GO - Molecular functioni
- histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 120845, 65 interactors |
CORUMi | Q9NVP2 |
DIPi | DIP-29242N |
IntActi | Q9NVP2, 43 interactors |
MINTi | Q9NVP2 |
STRINGi | 9606.ENSP00000263382 |
Miscellaneous databases
RNActi | Q9NVP2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9NVP2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 156 | Interaction with histone H3By similarityAdd BLAST | 156 | |
Regioni | 1 – 155 | Interaction with CHAF1BAdd BLAST | 155 |
Sequence similaritiesi
Belongs to the ASF1 family.Curated
Phylogenomic databases
eggNOGi | KOG3265, Eukaryota |
GeneTreei | ENSGT00390000004692 |
HOGENOMi | CLU_060354_1_2_1 |
InParanoidi | Q9NVP2 |
OMAi | SCTPVKG |
OrthoDBi | 1334998at2759 |
PhylomeDBi | Q9NVP2 |
TreeFami | TF106429 |
Family and domain databases
Gene3Di | 2.60.40.1490, 1 hit |
InterProi | View protein in InterPro IPR006818, ASF1-like IPR036747, ASF1-like_sf |
PANTHERi | PTHR12040, PTHR12040, 1 hit |
Pfami | View protein in Pfam PF04729, ASF1_hist_chap, 1 hit |
SUPFAMi | SSF101546, SSF101546, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9NVP2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES
60 70 80 90 100
EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT
110 120 130 140 150
YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH
160 170 180 190 200
INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD
CI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketK7ES22 | K7ES22_HUMAN | Histone chaperone ASF1B | ASF1B | 143 | Annotation score: | ||
K7ELW9 | K7ELW9_HUMAN | Histone chaperone ASF1B | ASF1B | 165 | Annotation score: | ||
K7EIL9 | K7EIL9_HUMAN | Histone chaperone ASF1B | ASF1B | 81 | Annotation score: | ||
K7EM93 | K7EM93_HUMAN | Histone chaperone ASF1B | ASF1B | 48 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 11 | V → A in BAA91602 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 23 | R → Q in BAD96800 (Ref. 5) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF279307 mRNA Translation: AAK82973.1 AB104486 mRNA Translation: BAC87709.1 CR457235 mRNA Translation: CAG33516.1 AK001288 mRNA Translation: BAA91602.1 AK001466 mRNA Translation: BAA91708.1 AK223080 mRNA Translation: BAD96800.1 AC022098 Genomic DNA No translation available. BC007726 mRNA Translation: AAH07726.1 BC010014 mRNA Translation: AAH10014.1 BC036521 mRNA Translation: AAH36521.1 |
CCDSi | CCDS12306.1 |
RefSeqi | NP_060624.1, NM_018154.2 |
Genome annotation databases
Ensembli | ENST00000263382; ENSP00000263382; ENSG00000105011 ENST00000672225; ENSP00000499863; ENSG00000288210 |
GeneIDi | 55723 |
KEGGi | hsa:55723 |
UCSCi | uc002mye.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF279307 mRNA Translation: AAK82973.1 AB104486 mRNA Translation: BAC87709.1 CR457235 mRNA Translation: CAG33516.1 AK001288 mRNA Translation: BAA91602.1 AK001466 mRNA Translation: BAA91708.1 AK223080 mRNA Translation: BAD96800.1 AC022098 Genomic DNA No translation available. BC007726 mRNA Translation: AAH07726.1 BC010014 mRNA Translation: AAH10014.1 BC036521 mRNA Translation: AAH36521.1 |
CCDSi | CCDS12306.1 |
RefSeqi | NP_060624.1, NM_018154.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5BNX | X-ray | 2.31 | D | 1-158 | [»] | |
5BO0 | X-ray | 2.91 | D | 1-158 | [»] | |
SMRi | Q9NVP2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120845, 65 interactors |
CORUMi | Q9NVP2 |
DIPi | DIP-29242N |
IntActi | Q9NVP2, 43 interactors |
MINTi | Q9NVP2 |
STRINGi | 9606.ENSP00000263382 |
PTM databases
iPTMneti | Q9NVP2 |
MetOSitei | Q9NVP2 |
PhosphoSitePlusi | Q9NVP2 |
SwissPalmi | Q9NVP2 |
Genetic variation databases
BioMutai | ASF1B |
Proteomic databases
CPTACi | CPTAC-953 |
EPDi | Q9NVP2 |
jPOSTi | Q9NVP2 |
MassIVEi | Q9NVP2 |
MaxQBi | Q9NVP2 |
PaxDbi | Q9NVP2 |
PeptideAtlasi | Q9NVP2 |
PRIDEi | Q9NVP2 |
ProteomicsDBi | 82838 |
Protocols and materials databases
ABCDi | Q9NVP2, 1 sequenced antibody |
Antibodypediai | 26631, 304 antibodies |
DNASUi | 55723 |
Genome annotation databases
Ensembli | ENST00000263382; ENSP00000263382; ENSG00000105011 ENST00000672225; ENSP00000499863; ENSG00000288210 |
GeneIDi | 55723 |
KEGGi | hsa:55723 |
UCSCi | uc002mye.4, human |
Organism-specific databases
CTDi | 55723 |
DisGeNETi | 55723 |
GeneCardsi | ASF1B |
HGNCi | HGNC:20996, ASF1B |
HPAi | ENSG00000105011, Group enriched (blood, lymphoid tissue, testis) |
MIMi | 609190, gene |
neXtProti | NX_Q9NVP2 |
OpenTargetsi | ENSG00000105011 |
PharmGKBi | PA134931112 |
VEuPathDBi | HostDB:ENSG00000105011.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3265, Eukaryota |
GeneTreei | ENSGT00390000004692 |
HOGENOMi | CLU_060354_1_2_1 |
InParanoidi | Q9NVP2 |
OMAi | SCTPVKG |
OrthoDBi | 1334998at2759 |
PhylomeDBi | Q9NVP2 |
TreeFami | TF106429 |
Enzyme and pathway databases
PathwayCommonsi | Q9NVP2 |
SIGNORi | Q9NVP2 |
Miscellaneous databases
BioGRID-ORCSi | 55723, 184 hits in 1004 CRISPR screens |
ChiTaRSi | ASF1B, human |
GeneWikii | ASF1B |
GenomeRNAii | 55723 |
Pharosi | Q9NVP2, Tbio |
PROi | PR:Q9NVP2 |
RNActi | Q9NVP2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000105011, Expressed in oocyte and 132 other tissues |
ExpressionAtlasi | Q9NVP2, baseline and differential |
Genevisiblei | Q9NVP2, HS |
Family and domain databases
Gene3Di | 2.60.40.1490, 1 hit |
InterProi | View protein in InterPro IPR006818, ASF1-like IPR036747, ASF1-like_sf |
PANTHERi | PTHR12040, PTHR12040, 1 hit |
Pfami | View protein in Pfam PF04729, ASF1_hist_chap, 1 hit |
SUPFAMi | SSF101546, SSF101546, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASF1B_HUMAN | |
Accessioni | Q9NVP2Primary (citable) accession number: Q9NVP2 Secondary accession number(s): Q53G51, Q9NVZ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 17, 2007 |
Last sequence update: | October 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families