ASF1B

Functionⁱ

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.5 Publications

GO - Molecular functionⁱ

histone binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell differentiation Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly
Source: UniProtKB
Inferred from direct assayⁱ
- 14718166
DNA replication-independent nucleosome assembly
Source: UniProtKB
Inferred from direct assayⁱ
- 14718166
multicellular organism development Source: UniProtKB-KW
regulation of transcription, DNA-templated Source: UniProtKB-KW
spermatogenesis Source: UniProtKB-KW
transcription, DNA-templated Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone, Chromatin regulator, Developmental protein
Biological process	Differentiation, Spermatogenesis, Transcription, Transcription regulation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Histone chaperone ASF1B Alternative name(s): Anti-silencing function protein 1 homolog B Short name: hAsf1 Short name: hAsf1b CCG1-interacting factor A-II Short name: CIA-II Short name: hCIA-II
Gene namesⁱ	Name:ASF1B
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000105011.8
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:20996 ASF1B
MIMⁱ	609190 gene
neXtProtⁱ	NX_Q9NVP2

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	36	D → A: Abolishes CDAN1 interaction. 1 Publication		1
Mutagenesisⁱ	37	D → A: Abolishes CDAN1 interaction. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	55723
Open Targets More...OpenTargetsⁱ	ENSG00000105011
PharmGKBⁱ	PA134931112

Polymorphism and mutation databases

BioMutaⁱ	ASF1B

BioMutaⁱ

ASF1B

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000284015	1 – 202	Histone chaperone ASF1BAdd BLAST		202

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	198	Phosphoserine; by TLK21 Publication		1

Post-translational modificationⁱ

Phosphorylated by TLK1 and TLK2.2 Publications

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q9NVP2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NVP2
PaxDbⁱ	Q9NVP2
PeptideAtlas More...PeptideAtlasⁱ	Q9NVP2
PRIDEⁱ	Q9NVP2
ProteomicsDBⁱ	82838

PTM databases

iPTMnetⁱ	Q9NVP2
PhosphoSitePlusⁱ	Q9NVP2

Expressionⁱ

Tissue specificityⁱ

Highly expressed in testis and at lower levels in colon, small intestine and thymus.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000105011 Expressed in 114 organ(s), highest expression level in oocyte
CleanExⁱ	HS_ASF1B
ExpressionAtlasⁱ	Q9NVP2 baseline and differential
Genevisibleⁱ	Q9NVP2 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA054036

HPAⁱ

HPA054036

Interactionⁱ

Subunit structureⁱ

Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Interacts with CDAN1. Found in a cytosolic complex with CDAN1, ASF1A, IPO4 and histones H3.1 and H4. Interacts with CREBBP.9 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CHAF1B	Q13112	2	EBI-1055650,EBI-1052944
H3F3B	P84243	4	EBI-1055650,EBI-120658
HIRA	P54198	3	EBI-1055650,EBI-372342

GO - Molecular functionⁱ

histone binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	120845, 63 interactors
CORUMⁱ	Q9NVP2
Database of interacting proteins More...DIPⁱ	DIP-29242N
IntActⁱ	Q9NVP2, 22 interactors
Molecular INTeraction database More...MINTⁱ	Q9NVP2
STRINGⁱ	9606.ENSP00000263382

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	3 – 11	Combined sources	9
Beta strandⁱ	15 – 17	Combined sources	3
Beta strandⁱ	22 – 32	Combined sources	11
Beta strandⁱ	34 – 36	Combined sources	3
Beta strandⁱ	38 – 45	Combined sources	8
Helixⁱ	51 – 53	Combined sources	3
Beta strandⁱ	54 – 62	Combined sources	9
Beta strandⁱ	67 – 76	Combined sources	10
Helixⁱ	81 – 83	Combined sources	3
Helixⁱ	86 – 89	Combined sources	4
Beta strandⁱ	90 – 101	Combined sources	12
Beta strandⁱ	104 – 117	Combined sources	14
Helixⁱ	120 – 124	Combined sources	5
Helixⁱ	132 – 134	Combined sources	3
Beta strandⁱ	135 – 139	Combined sources	5
Beta strandⁱ	145 – 148	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9NVP2
SMRⁱ	Q9NVP2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 156	Interaction with histone H3By similarityAdd BLAST		156
Regionⁱ	1 – 155	Interaction with CHAF1BAdd BLAST		155

Sequence similaritiesⁱ

Belongs to the ASF1 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3265 Eukaryota COG5137 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000004692
HOGENOMⁱ	HOG000197425
HOVERGENⁱ	HBG105617
InParanoidⁱ	Q9NVP2
KEGG Orthology (KO) More...KOⁱ	K10753
OMAⁱ	HINWEGC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0K07
PhylomeDBⁱ	Q9NVP2
TreeFamⁱ	TF106429

Family and domain databases

Gene3Dⁱ	2.60.40.1490, 1 hit
InterProⁱ	View protein in InterPro IPR006818 ASF1-like IPR036747 ASF1-like_sf
PANTHERⁱ	PTHR12040 PTHR12040, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04729 ASF1_hist_chap, 1 hit
SUPFAMⁱ	SSF101546 SSF101546, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.ⁱShow all

Q9NVP2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES 
        60         70         80         90        100
EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT 
       110        120        130        140        150
YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH 
       160        170        180        190        200
INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD 

CI

Length:202

Mass (Da):22,434

Last modified:October 1, 2000 - v1

Checksum:ⁱBD62F726610E3A70

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation
K7ES22	K7ES22_HUMAN	Histone chaperone ASF1B Histone chaperone ASF1B	ASF1B	143	Annotation score:
K7EM93	K7EM93_HUMAN	Histone chaperone ASF1B Histone chaperone ASF1B	ASF1B	48	Annotation score:
K7ELW9	K7ELW9_HUMAN	Histone chaperone ASF1B Histone chaperone ASF1B	ASF1B	165	Annotation score:
K7EIL9	K7EIL9_HUMAN	Histone chaperone ASF1B Histone chaperone ASF1B	ASF1B	81	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	11	V → A in BAA91602 (PubMed:14702039).Curated		1
Sequence conflictⁱ	23	R → Q in BAD96800 (Ref. 5) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF279307 mRNA Translation: AAK82973.1 AB104486 mRNA Translation: BAC87709.1 CR457235 mRNA Translation: CAG33516.1 AK001288 mRNA Translation: BAA91602.1 AK001466 mRNA Translation: BAA91708.1 AK223080 mRNA Translation: BAD96800.1 AC022098 Genomic DNA No translation available. BC007726 mRNA Translation: AAH07726.1 BC010014 mRNA Translation: AAH10014.1 BC036521 mRNA Translation: AAH36521.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12306.1
NCBI Reference Sequences More...RefSeqⁱ	NP_060624.1, NM_018154.2
UniGeneⁱ	Hs.26516

Genome annotation databases

Ensemblⁱ	ENST00000263382; ENSP00000263382; ENSG00000105011
GeneIDⁱ	55723
KEGGⁱ	hsa:55723
UCSC genome browser More...UCSCⁱ	uc002mye.4 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NVP2	ASF1 anti-silencing function 1 homolog B (S. cerevisiae), isoform CRA_a		HUMAN		202	UniRef100_Q9NVP2
ASF1 anti-silencing function 1 homolog B		PANTR		202
Anti-silencing function 1B histone chaperone		PANPA		202
UPI000645454C		HUMAN		158

Protein	Similar proteins		Species	Score	Length	Source
Q9NVP2	Histone chaperone ASF1B		BOVIN		202	UniRef90_Q9NVP2
Histone chaperone ASF1B		MOUSE		202
ASF1 anti-silencing function 1 homolog B (S. cerevisiae), isoform CRA_a		HUMAN		202
ASF1 anti-silencing function 1 homolog B		PANTR		202
Anti-silencing function 1B histone chaperone		PANPA		202
+59

Protein	Similar proteins		Species	Score	Length	Source
Q9NVP2	Histone chaperone ASF1B		MOUSE		202	UniRef50_Q9NVP2
Histone chaperone ASF1B		BOVIN		202
ASF1 anti-silencing function 1 homolog B (S. cerevisiae), isoform CRA_a		HUMAN		202
ASF1 anti-silencing function 1 homolog B		PANTR		202
Anti-silencing function 1B histone chaperone		PANPA		202
+133

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF279307 mRNA Translation: AAK82973.1 AB104486 mRNA Translation: BAC87709.1 CR457235 mRNA Translation: CAG33516.1 AK001288 mRNA Translation: BAA91602.1 AK001466 mRNA Translation: BAA91708.1 AK223080 mRNA Translation: BAD96800.1 AC022098 Genomic DNA No translation available. BC007726 mRNA Translation: AAH07726.1 BC010014 mRNA Translation: AAH10014.1 BC036521 mRNA Translation: AAH36521.1
CCDSⁱ	CCDS12306.1
RefSeqⁱ	NP_060624.1, NM_018154.2
UniGeneⁱ	Hs.26516

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5BNX	X-ray	2.31	D	1-158	[»]
	5BO0	X-ray	2.91	D	1-158	[»]
ProteinModelPortalⁱ	Q9NVP2
SMRⁱ	Q9NVP2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	120845, 63 interactors
CORUMⁱ	Q9NVP2
DIPⁱ	DIP-29242N
IntActⁱ	Q9NVP2, 22 interactors
MINTⁱ	Q9NVP2
STRINGⁱ	9606.ENSP00000263382

PTM databases

iPTMnetⁱ	Q9NVP2
PhosphoSitePlusⁱ	Q9NVP2

Polymorphism and mutation databases

BioMutaⁱ	ASF1B

BioMutaⁱ

ASF1B

Proteomic databases

EPDⁱ	Q9NVP2
MaxQBⁱ	Q9NVP2
PaxDbⁱ	Q9NVP2
PeptideAtlasⁱ	Q9NVP2
PRIDEⁱ	Q9NVP2
ProteomicsDBⁱ	82838

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	55723
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000263382; ENSP00000263382; ENSG00000105011
GeneIDⁱ	55723
KEGGⁱ	hsa:55723
UCSCⁱ	uc002mye.4 human

Organism-specific databases

CTDⁱ	55723
DisGeNETⁱ	55723
EuPathDBⁱ	HostDB:ENSG00000105011.8
GeneCardsⁱ	ASF1B
HGNCⁱ	HGNC:20996 ASF1B
HPAⁱ	HPA054036
MIMⁱ	609190 gene
neXtProtⁱ	NX_Q9NVP2
OpenTargetsⁱ	ENSG00000105011
PharmGKBⁱ	PA134931112
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3265 Eukaryota COG5137 LUCA
GeneTreeⁱ	ENSGT00390000004692
HOGENOMⁱ	HOG000197425
HOVERGENⁱ	HBG105617
InParanoidⁱ	Q9NVP2
KOⁱ	K10753
OMAⁱ	HINWEGC
OrthoDBⁱ	EOG091G0K07
PhylomeDBⁱ	Q9NVP2
TreeFamⁱ	TF106429

Miscellaneous databases

ChiTaRSⁱ	ASF1B human
GeneWikiⁱ	ASF1B
GenomeRNAiⁱ	55723
Protein Ontology More...PROⁱ	PR:Q9NVP2
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000105011 Expressed in 114 organ(s), highest expression level in oocyte
CleanExⁱ	HS_ASF1B
ExpressionAtlasⁱ	Q9NVP2 baseline and differential
Genevisibleⁱ	Q9NVP2 HS

Family and domain databases

Gene3Dⁱ	2.60.40.1490, 1 hit
InterProⁱ	View protein in InterPro IPR006818 ASF1-like IPR036747 ASF1-like_sf
PANTHERⁱ	PTHR12040 PTHR12040, 1 hit
Pfamⁱ	View protein in Pfam PF04729 ASF1_hist_chap, 1 hit
SUPFAMⁱ	SSF101546 SSF101546, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ASF1B_HUMAN
Accessionⁱ	Q9NVP2Primary (citable) accession number: Q9NVP2 Secondary accession number(s): Q53G51, Q9NVZ0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 17, 2007
	Last sequence update:	October 1, 2000
	Last modified:	September 12, 2018
	This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniProtKB - Q9NVP2 (ASF1B_HUMAN)

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Histone chaperone ASF1B

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Computationally mapped potential isoform sequencesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ