UniProtKB - Q9NVJ2 (ARL8B_HUMAN)
Protein
ADP-ribosylation factor-like protein 8B
Gene
ARL8B
Organism
Homo sapiens (Human)
Status
Functioni
Plays a role in lysosome motility (PubMed:16537643, PubMed:25898167). In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation (PubMed:15331635).By similarity3 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 29 – 35 | GTP | 7 | |
Nucleotide bindingi | 71 – 75 | GTPBy similarity | 5 | |
Nucleotide bindingi | 130 – 133 | GTP | 4 |
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- beta-tubulin binding Source: UniProtKB
- GDP binding Source: UniProtKB
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
GO - Biological processi
- anterograde axonal transport Source: Ensembl
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- chromosome segregation Source: UniProtKB
- endosomal transport Source: Ensembl
- lysosome localization Source: UniProtKB
- protein transport Source: UniProtKB-KW
Keywordsi
Biological process | Cell cycle, Cell division, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9NVJ2 |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor-like protein 8BAlternative name(s): ADP-ribosylation factor-like protein 10C Novel small G protein indispensable for equal chromosome segregation 1 |
Gene namesi | Name:ARL8B Synonyms:ARL10C, GIE1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000134108.12 |
HGNCi | HGNC:25564, ARL8B |
MIMi | 616596, gene |
neXtProti | NX_Q9NVJ2 |
Subcellular locationi
Lysosome
- Lysosome membrane 1 Publication
Cytoskeleton
- spindle 1 Publication
Endosome
- Late endosome membrane 1 Publication
Other locations
Note: Localizes with microtubules at the spindle mid-zone during mitosis.1 Publication
Cytoskeleton
- spindle midzone Source: UniProtKB
Endosome
- late endosome membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
Other locations
- axon cytoplasm Source: GOC
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
- midbody Source: UniProtKB
- synapse Source: UniProtKB-SubCell
- vacuolar membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Intramembranei | 1 – 19 | Note=Mediates targeting to membranesAdd BLAST | 19 |
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Endosome, Lysosome, Membrane, SynapsePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | L → A: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation. 1 Publication | 1 | |
Mutagenesisi | 2 | L → F: No effect on localization and acetylation. 1 Publication | 1 | |
Mutagenesisi | 5 – 12 | ISRLLDWF → ASRALDWA: Diffuse cytoplasmic distribution and loss of localization to lysosomes. No effect on acetylation. 1 Publication | 8 | |
Mutagenesisi | 34 | T → N: Preferentially binds GDP. Alters chromosome segregation. 1 Publication | 1 | |
Mutagenesisi | 49 – 58 | Missing : Alters chromosome segregation. 1 Publication | 10 | |
Mutagenesisi | 70 | W → R: Preferentially binds GTP. 1 Publication | 1 | |
Mutagenesisi | 74 – 85 | Missing : Alters chromosome segregation. 1 PublicationAdd BLAST | 12 | |
Mutagenesisi | 75 | Q → L: Prevents GTP hydrolysis. No effect on localization. Alters lysosomes cellular distribution and motility. 1 Publication | 1 | |
Mutagenesisi | 130 | N → I: Loss of GTP/GDP-binding. Affects chromosome segregation. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 55207 |
OpenTargetsi | ENSG00000134108 |
PharmGKBi | PA134959531 |
Miscellaneous databases
Pharosi | Q9NVJ2, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295962 |
Polymorphism and mutation databases
BioMutai | ARL8B |
DMDMi | 74752996 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000232921 | 1 – 186 | ADP-ribosylation factor-like protein 8BAdd BLAST | 186 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9NVJ2 |
jPOSTi | Q9NVJ2 |
MassIVEi | Q9NVJ2 |
MaxQBi | Q9NVJ2 |
PaxDbi | Q9NVJ2 |
PeptideAtlasi | Q9NVJ2 |
PRIDEi | Q9NVJ2 |
ProteomicsDBi | 4283 82817 [Q9NVJ2-1] |
TopDownProteomicsi | Q9NVJ2-1 [Q9NVJ2-1] |
PTM databases
iPTMneti | Q9NVJ2 |
PhosphoSitePlusi | Q9NVJ2 |
SwissPalmi | Q9NVJ2 |
Expressioni
Tissue specificityi
Ubiquitously expressed.1 Publication
Gene expression databases
Bgeei | ENSG00000134108, Expressed in middle temporal gyrus and 241 other tissues |
ExpressionAtlasi | Q9NVJ2, baseline and differential |
Genevisiblei | Q9NVJ2, HS |
Organism-specific databases
HPAi | ENSG00000134108, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsQ9NVJ2
With | #Exp. | IntAct |
---|---|---|
CIDEB [Q9UHD4] | 3 | EBI-718376,EBI-7062247 |
SLC30A8 [Q8IWU4] | 3 | EBI-718376,EBI-10262251 |
TMEM14B [Q9NUH8] | 3 | EBI-718376,EBI-8638294 |
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- beta-tubulin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 120503, 51 interactors |
IntActi | Q9NVJ2, 33 interactors |
MINTi | Q9NVJ2 |
STRINGi | 9606.ENSP00000256496 |
Miscellaneous databases
RNActi | Q9NVJ2, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9NVJ2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NVJ2 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0075, Eukaryota |
GeneTreei | ENSGT00940000154861 |
HOGENOMi | CLU_040729_10_0_1 |
InParanoidi | Q9NVJ2 |
OMAi | PVSCYSC |
PhylomeDBi | Q9NVJ2 |
TreeFami | TF105470 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR006689, Small_GTPase_ARF/SAR |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NVJ2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLALISRLLD WFRSLFWKEE MELTLVGLQY SGKTTFVNVI ASGQFSEDMI
60 70 80 90 100
PTVGFNMRKV TKGNVTIKIW DIGGQPRFRS MWERYCRGVN AIVYMIDAAD
110 120 130 140 150
REKIEASRNE LHNLLDKPQL QGIPVLVLGN KRDLPNALDE KQLIEKMNLS
160 170 180
AIQDREICCY SISCKEKDNI DITLQWLIQH SKSRRS
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF2Z344 | F2Z344_HUMAN | ADP-ribosylation factor-like protei... | ARL8B | 103 | Annotation score: | ||
F8WAW5 | F8WAW5_HUMAN | ADP-ribosylation factor-like protei... | ARL8B | 102 | Annotation score: | ||
A0A6Q8PGM9 | A0A6Q8PGM9_HUMAN | ADP-ribosylation factor-like protei... | ARL8B | 21 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056238 | 125 – 186 | VLVLG…KSRRS → ISHFSGLFSIQNLEEAEASP EVFQSFLAIILELLSVPLK in isoform 2. 1 PublicationAdd BLAST | 62 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB118751 mRNA Translation: BAD23992.1 AK001564 mRNA Translation: BAA91759.1 AK295465 mRNA Translation: BAG58397.1 CR457264 mRNA Translation: CAG33545.1 AC021999 Genomic DNA No translation available. AC026202 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW63919.1 BC013131 mRNA Translation: AAH13131.1 BC063125 mRNA Translation: AAH63125.1 |
CCDSi | CCDS2566.1 [Q9NVJ2-1] |
RefSeqi | NP_060654.1, NM_018184.2 [Q9NVJ2-1] |
Genome annotation databases
Ensembli | ENST00000256496; ENSP00000256496; ENSG00000134108 [Q9NVJ2-1] ENST00000419534; ENSP00000402996; ENSG00000134108 [Q9NVJ2-2] ENST00000611208; ENSP00000479202; ENSG00000134108 [Q9NVJ2-1] |
GeneIDi | 55207 |
KEGGi | hsa:55207 |
UCSCi | uc003bqg.4, human [Q9NVJ2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB118751 mRNA Translation: BAD23992.1 AK001564 mRNA Translation: BAA91759.1 AK295465 mRNA Translation: BAG58397.1 CR457264 mRNA Translation: CAG33545.1 AC021999 Genomic DNA No translation available. AC026202 Genomic DNA No translation available. CH471055 Genomic DNA Translation: EAW63919.1 BC013131 mRNA Translation: AAH13131.1 BC063125 mRNA Translation: AAH63125.1 |
CCDSi | CCDS2566.1 [Q9NVJ2-1] |
RefSeqi | NP_060654.1, NM_018184.2 [Q9NVJ2-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2AL7 | X-ray | 1.85 | A | 18-184 | [»] | |
SMRi | Q9NVJ2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120503, 51 interactors |
IntActi | Q9NVJ2, 33 interactors |
MINTi | Q9NVJ2 |
STRINGi | 9606.ENSP00000256496 |
Chemistry databases
ChEMBLi | CHEMBL4295962 |
PTM databases
iPTMneti | Q9NVJ2 |
PhosphoSitePlusi | Q9NVJ2 |
SwissPalmi | Q9NVJ2 |
Polymorphism and mutation databases
BioMutai | ARL8B |
DMDMi | 74752996 |
Proteomic databases
EPDi | Q9NVJ2 |
jPOSTi | Q9NVJ2 |
MassIVEi | Q9NVJ2 |
MaxQBi | Q9NVJ2 |
PaxDbi | Q9NVJ2 |
PeptideAtlasi | Q9NVJ2 |
PRIDEi | Q9NVJ2 |
ProteomicsDBi | 4283 82817 [Q9NVJ2-1] |
TopDownProteomicsi | Q9NVJ2-1 [Q9NVJ2-1] |
Protocols and materials databases
Antibodypediai | 25235, 149 antibodies |
Genome annotation databases
Ensembli | ENST00000256496; ENSP00000256496; ENSG00000134108 [Q9NVJ2-1] ENST00000419534; ENSP00000402996; ENSG00000134108 [Q9NVJ2-2] ENST00000611208; ENSP00000479202; ENSG00000134108 [Q9NVJ2-1] |
GeneIDi | 55207 |
KEGGi | hsa:55207 |
UCSCi | uc003bqg.4, human [Q9NVJ2-1] |
Organism-specific databases
CTDi | 55207 |
DisGeNETi | 55207 |
EuPathDBi | HostDB:ENSG00000134108.12 |
GeneCardsi | ARL8B |
HGNCi | HGNC:25564, ARL8B |
HPAi | ENSG00000134108, Low tissue specificity |
MIMi | 616596, gene |
neXtProti | NX_Q9NVJ2 |
OpenTargetsi | ENSG00000134108 |
PharmGKBi | PA134959531 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0075, Eukaryota |
GeneTreei | ENSGT00940000154861 |
HOGENOMi | CLU_040729_10_0_1 |
InParanoidi | Q9NVJ2 |
OMAi | PVSCYSC |
PhylomeDBi | Q9NVJ2 |
TreeFami | TF105470 |
Enzyme and pathway databases
PathwayCommonsi | Q9NVJ2 |
Miscellaneous databases
BioGRID-ORCSi | 55207, 30 hits in 846 CRISPR screens |
ChiTaRSi | ARL8B, human |
EvolutionaryTracei | Q9NVJ2 |
GeneWikii | ARL8B |
GenomeRNAii | 55207 |
Pharosi | Q9NVJ2, Tbio |
PROi | PR:Q9NVJ2 |
RNActi | Q9NVJ2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000134108, Expressed in middle temporal gyrus and 241 other tissues |
ExpressionAtlasi | Q9NVJ2, baseline and differential |
Genevisiblei | Q9NVJ2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR006689, Small_GTPase_ARF/SAR |
Pfami | View protein in Pfam PF00025, Arf, 1 hit |
PRINTSi | PR00328, SAR1GTPBP |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51417, ARF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARL8B_HUMAN | |
Accessioni | Q9NVJ2Primary (citable) accession number: Q9NVJ2 Secondary accession number(s): B4DI85 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 18, 2006 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families