UniProtKB - Q9NUU7 (DD19A_HUMAN)
Protein
ATP-dependent RNA helicase DDX19A
Gene
DDX19A
Organism
Homo sapiens (Human)
Status
Functioni
ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19 functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.By similarity
Catalytic activityi
- EC:3.6.4.13By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | ATPBy similarity | 1 | |
Binding sitei | 428 | ATPBy similarity | 1 | |
Binding sitei | 431 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 137 – 144 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- RNA binding Source: GO_Central
- RNA helicase activity Source: GO_Central
GO - Biological processi
- poly(A)+ mRNA export from nucleus Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9NUU7 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DDX19A (EC:3.6.4.13By similarity)Alternative name(s): DDX19-like protein DEAD box protein 19A |
Gene namesi | Name:DDX19A Synonyms:DDX19L |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:25628, DDX19A |
neXtProti | NX_Q9NUU7 |
VEuPathDBi | HostDB:ENSG00000168872.16 |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
Other locations
- Cytoplasm By similarity
Note: Associates with the nuclear pore complex cytoplasmic fibrils.By similarity
Nucleus
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: GO_Central
Other locations
- cytoplasmic stress granule Source: GO_Central
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55308 |
OpenTargetsi | ENSG00000168872 |
PharmGKBi | PA134894996 |
Miscellaneous databases
Pharosi | Q9NUU7, Tdark |
Genetic variation databases
BioMutai | DDX19A |
DMDMi | 73919226 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000055021 | 2 – 478 | ATP-dependent RNA helicase DDX19AAdd BLAST | 477 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Cross-linki | 26 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources | ||
Cross-linki | 26 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources | ||
Modified residuei | 42 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9NUU7 |
jPOSTi | Q9NUU7 |
MassIVEi | Q9NUU7 |
MaxQBi | Q9NUU7 |
PaxDbi | Q9NUU7 |
PeptideAtlasi | Q9NUU7 |
PRIDEi | Q9NUU7 |
ProteomicsDBi | 4981 82721 [Q9NUU7-1] |
PTM databases
iPTMneti | Q9NUU7 |
MetOSitei | Q9NUU7 |
PhosphoSitePlusi | Q9NUU7 |
SwissPalmi | Q9NUU7 |
Expressioni
Gene expression databases
Bgeei | ENSG00000168872, Expressed in gastrocnemius and 127 other tissues |
ExpressionAtlasi | Q9NUU7, baseline and differential |
Genevisiblei | Q9NUU7, HS |
Organism-specific databases
HPAi | ENSG00000168872, Low tissue specificity |
Interactioni
Binary interactionsi
Hide detailsQ9NUU7
With | #Exp. | IntAct |
---|---|---|
CTIF - isoform 2 [O43310-2] | 4 | EBI-740301,EBI-12180013 |
MIF4GD [A9UHW6] | 7 | EBI-740301,EBI-373498 |
MIF4GD - isoform 2 [A9UHW6-2] | 3 | EBI-740301,EBI-9118295 |
Protein-protein interaction databases
BioGRIDi | 120592, 46 interactors |
IntActi | Q9NUU7, 27 interactors |
MINTi | Q9NUU7 |
STRINGi | 9606.ENSP00000306117 |
Miscellaneous databases
RNActi | Q9NUU7, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 124 – 294 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 171 | |
Domaini | 305 – 473 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 169 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 299 | N-terminal lobeBy similarityAdd BLAST | 298 | |
Regioni | 54 – 67 | N-terminal helixBy similarityAdd BLAST | 14 | |
Regioni | 300 – 478 | C-terminal lobeBy similarityAdd BLAST | 179 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 91 – 119 | Q motifAdd BLAST | 29 | |
Motifi | 241 – 244 | DEAD box | 4 |
Domaini
The N-terminal extension helix acts as an autoinhibitory domain, preventing ATP hydrolysis, unless the N-terminus of the protein is displaced by RNA binding, allowing cleft closure to bring key side chains into position for catalysis.By similarity
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0332, Eukaryota |
GeneTreei | ENSGT00940000154417 |
InParanoidi | Q9NUU7 |
OMAi | INFELPM |
OrthoDBi | 608788at2759 |
PhylomeDBi | Q9NUU7 |
TreeFami | TF314957 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NUU7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATDSWALAV DEQEAAVKSM TNLQIKEEKV KADTNGIIKT STTAEKTDEE
60 70 80 90 100
EKEDRAAQSL LNKLIRSNLV DNTNQVEVLQ RDPNSPLYSV KSFEELRLKP
110 120 130 140 150
QLLQGVYAMG FNRPSKIQEN ALPMMLAEPP QNLIAQSQSG TGKTAAFVLA
160 170 180 190 200
MLSRVEPSDR YPQCLCLSPT YELALQTGKV IEQMGKFYPE LKLAYAVRGN
210 220 230 240 250
KLERGQKISE QIVIGTPGTV LDWCSKLKFI DPKKIKVFVL DEADVMIATQ
260 270 280 290 300
GHQDQSIRIQ RMLPRNCQML LFSATFEDSV WKFAQKVVPD PNVIKLKREE
310 320 330 340 350
ETLDTIKQYY VLCSSRDEKF QALCNLYGAI TIAQAMIFCH TRKTASWLAA
360 370 380 390 400
ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE GKEKVLVTTN VCARGIDVEQ
410 420 430 440 450
VSVVINFDLP VDKDGNPDNE TYLHRIGRTG RFGKRGLAVN MVDSKHSMNI
460 470
LNRIQEHFNK KIERLDTDDL DEIEKIAN
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketI3L352 | I3L352_HUMAN | RNA helicase | DDX19A | 396 | Annotation score: | ||
H3BTB3 | H3BTB3_HUMAN | RNA helicase | DDX19A | 129 | Annotation score: | ||
I3L0H8 | I3L0H8_HUMAN | ATP-dependent RNA helicase DDX19A | DDX19A | 447 | Annotation score: | ||
H3BSL8 | H3BSL8_HUMAN | ATP-dependent RNA helicase DDX19A | DDX19A | 78 | Annotation score: | ||
H3BP36 | H3BP36_HUMAN | ATP-dependent RNA helicase DDX19A | DDX19A | 47 | Annotation score: | ||
J3QRH0 | J3QRH0_HUMAN | ATP-dependent RNA helicase DDX19A | DDX19A | 64 | Annotation score: | ||
H3BP50 | H3BP50_HUMAN | ATP-dependent RNA helicase DDX19A | DDX19A | 51 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 463 | E → G in BAD96982 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056954 | 1 – 98 | MATDS…EELRL → MSGTFLIR in isoform 2. 1 PublicationAdd BLAST | 98 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK001988 mRNA Translation: BAA92022.1 AK299504 mRNA Translation: BAG61459.1 AK223262 mRNA Translation: BAD96982.1 AL832970 mRNA Translation: CAH10622.1 AC012184 Genomic DNA No translation available. CH471241 Genomic DNA Translation: EAW51826.1 BC005162 mRNA Translation: AAH05162.1 BC006544 mRNA Translation: AAH06544.1 BC137496 mRNA Translation: AAI37497.1 BC137497 mRNA Translation: AAI37498.1 |
CCDSi | CCDS10889.1 [Q9NUU7-1] |
RefSeqi | NP_001307451.1, NM_001320522.1 NP_001307454.1, NM_001320525.1 [Q9NUU7-2] NP_001307455.1, NM_001320526.1 NP_001307456.1, NM_001320527.1 NP_060802.1, NM_018332.4 [Q9NUU7-1] |
Genome annotation databases
Ensembli | ENST00000302243; ENSP00000306117; ENSG00000168872 [Q9NUU7-1] |
GeneIDi | 55308 |
KEGGi | hsa:55308 |
UCSCi | uc002eyv.4, human [Q9NUU7-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK001988 mRNA Translation: BAA92022.1 AK299504 mRNA Translation: BAG61459.1 AK223262 mRNA Translation: BAD96982.1 AL832970 mRNA Translation: CAH10622.1 AC012184 Genomic DNA No translation available. CH471241 Genomic DNA Translation: EAW51826.1 BC005162 mRNA Translation: AAH05162.1 BC006544 mRNA Translation: AAH06544.1 BC137496 mRNA Translation: AAI37497.1 BC137497 mRNA Translation: AAI37498.1 |
CCDSi | CCDS10889.1 [Q9NUU7-1] |
RefSeqi | NP_001307451.1, NM_001320522.1 NP_001307454.1, NM_001320525.1 [Q9NUU7-2] NP_001307455.1, NM_001320526.1 NP_001307456.1, NM_001320527.1 NP_060802.1, NM_018332.4 [Q9NUU7-1] |
3D structure databases
SMRi | Q9NUU7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 120592, 46 interactors |
IntActi | Q9NUU7, 27 interactors |
MINTi | Q9NUU7 |
STRINGi | 9606.ENSP00000306117 |
Protein family/group databases
TCDBi | 1.I.1.1.3, the nuclear pore complex (npc) family |
PTM databases
iPTMneti | Q9NUU7 |
MetOSitei | Q9NUU7 |
PhosphoSitePlusi | Q9NUU7 |
SwissPalmi | Q9NUU7 |
Genetic variation databases
BioMutai | DDX19A |
DMDMi | 73919226 |
Proteomic databases
EPDi | Q9NUU7 |
jPOSTi | Q9NUU7 |
MassIVEi | Q9NUU7 |
MaxQBi | Q9NUU7 |
PaxDbi | Q9NUU7 |
PeptideAtlasi | Q9NUU7 |
PRIDEi | Q9NUU7 |
ProteomicsDBi | 4981 82721 [Q9NUU7-1] |
Protocols and materials databases
Antibodypediai | 16355, 90 antibodies |
DNASUi | 55308 |
Genome annotation databases
Ensembli | ENST00000302243; ENSP00000306117; ENSG00000168872 [Q9NUU7-1] |
GeneIDi | 55308 |
KEGGi | hsa:55308 |
UCSCi | uc002eyv.4, human [Q9NUU7-1] |
Organism-specific databases
CTDi | 55308 |
DisGeNETi | 55308 |
GeneCardsi | DDX19A |
HGNCi | HGNC:25628, DDX19A |
HPAi | ENSG00000168872, Low tissue specificity |
neXtProti | NX_Q9NUU7 |
OpenTargetsi | ENSG00000168872 |
PharmGKBi | PA134894996 |
VEuPathDBi | HostDB:ENSG00000168872.16 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0332, Eukaryota |
GeneTreei | ENSGT00940000154417 |
InParanoidi | Q9NUU7 |
OMAi | INFELPM |
OrthoDBi | 608788at2759 |
PhylomeDBi | Q9NUU7 |
TreeFami | TF314957 |
Enzyme and pathway databases
PathwayCommonsi | Q9NUU7 |
Miscellaneous databases
BioGRID-ORCSi | 55308, 362 hits in 885 CRISPR screens |
ChiTaRSi | DDX19A, human |
GenomeRNAii | 55308 |
Pharosi | Q9NUU7, Tdark |
PROi | PR:Q9NUU7 |
RNActi | Q9NUU7, protein |
Gene expression databases
Bgeei | ENSG00000168872, Expressed in gastrocnemius and 127 other tissues |
ExpressionAtlasi | Q9NUU7, baseline and differential |
Genevisiblei | Q9NUU7, HS |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DD19A_HUMAN | |
Accessioni | Q9NUU7Primary (citable) accession number: Q9NUU7 Secondary accession number(s): B2RPL0, B4DRZ7, Q53FM0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 178 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - SIMILARITY comments
Index of protein domains and families