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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon

Gene

AGPAT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C15:0-C20:4 at the sn-1 position using C18:1-CoA as the acyl donor. Also acts on lysophosphatidylethanolamine using oleoyl-CoA, but not arachidonoyl-CoA, and lysophosphatidylinositol using arachidonoyl-CoA, but not oleoyl-CoA. Activity toward lysophosphatidylglycerol not detectable.1 Publication

Caution

It is uncertain whether Met-1 or Met-12 is the initiator.Curated

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Kineticsi

  1. KM=5.52 µM for LPA sn-1 C18:1
  2. KM=16.30 µM for C18:1-CoA
  1. Vmax=2.42 nmol/min/mg enzyme toward LPA sn-1 C18:1
  2. Vmax=1.22 nmol/min/mg enzyme toward C18:1-CoA

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), Glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial, Glycerol-3-phosphate acyltransferase 1, mitochondrial (DKFZp451B1115), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)
  2. Lysocardiolipin acyltransferase 1 (LCLAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3)
  3. Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase (cdsA), Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS08034-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA
UniPathwayi
UPA00557;UER00613

Chemistry databases

SwissLipidsiSLP:000000822

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (EC:2.3.1.51)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 5
Short name:
1-AGP acyltransferase 5
Short name:
1-AGPAT 5
Lysophosphatidic acid acyltransferase epsilon
Short name:
LPAAT-epsilon
Gene namesi
Name:AGPAT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000155189.11
HGNCiHGNC:20886 AGPAT5
MIMi614796 gene
neXtProtiNX_Q9NUQ2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei61 – 81HelicalSequence analysisAdd BLAST21
Transmembranei344 – 364HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55326
OpenTargetsiENSG00000155189
PharmGKBiPA134952751

Polymorphism and mutation databases

BioMutaiAGPAT5
DMDMi30923427

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002082001 – 3641-acyl-sn-glycerol-3-phosphate acyltransferase epsilonAdd BLAST364

Proteomic databases

EPDiQ9NUQ2
MaxQBiQ9NUQ2
PaxDbiQ9NUQ2
PeptideAtlasiQ9NUQ2
PRIDEiQ9NUQ2
ProteomicsDBi82706

PTM databases

iPTMnetiQ9NUQ2
PhosphoSitePlusiQ9NUQ2
SwissPalmiQ9NUQ2

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSG00000155189 Expressed in 231 organ(s), highest expression level in placenta
CleanExiHS_AGPAT5
ExpressionAtlasiQ9NUQ2 baseline and differential
GenevisibleiQ9NUQ2 HS

Organism-specific databases

HPAiHPA010644
HPA010950

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
UBQLN1Q9UMX05EBI-6916385,EBI-741480

Protein-protein interaction databases

BioGridi120607, 11 interactors
IntActiQ9NUQ2, 6 interactors
MINTiQ9NUQ2
STRINGi9606.ENSP00000285518

Structurei

3D structure databases

ProteinModelPortaliQ9NUQ2
SMRiQ9NUQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi93 – 98HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00890000139404
HOGENOMiHOG000007801
HOVERGENiHBG039632
InParanoidiQ9NUQ2
KOiK19007
OMAiDWIIADM
OrthoDBiEOG091G0C4Z
PhylomeDBiQ9NUQ2
TreeFamiTF314346

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9NUQ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLSLVLHTY SMRYLLPSVV LLGTAPTYVL AWGVWRLLSA FLPARFYQAL
60 70 80 90 100
DDRLYCVYQS MVLFFFENYT GVQILLYGDL PKNKENIIYL ANHQSTVDWI
110 120 130 140 150
VADILAIRQN ALGHVRYVLK EGLKWLPLYG CYFAQHGGIY VKRSAKFNEK
160 170 180 190 200
EMRNKLQSYV DAGTPMYLVI FPEGTRYNPE QTKVLSASQA FAAQRGLAVL
210 220 230 240 250
KHVLTPRIKA THVAFDCMKN YLDAIYDVTV VYEGKDDGGQ RRESPTMTEF
260 270 280 290 300
LCKECPKIHI HIDRIDKKDV PEEQEHMRRW LHERFEIKDK MLIEFYESPD
310 320 330 340 350
PERRKRFPGK SVNSKLSIKK TLPSMLILSG LTAGMLMTDA GRKLYVNTWI
360
YGTLLGCLWV TIKA
Length:364
Mass (Da):42,072
Last modified:May 16, 2003 - v3
Checksum:i90A0F87FC7C78081
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0YC22H0YC22_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT5
181Annotation score:
E5RH21E5RH21_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT5
88Annotation score:
H0YAW1H0YAW1_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT5
71Annotation score:

Sequence cautioni

The sequence BAA92069 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156L → V in BAA92069 (PubMed:11230166).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02269677Y → C. Corresponds to variant dbSNP:rs17077958Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375789 mRNA Translation: AAK54809.1
AL136587 mRNA Translation: CAB66522.1
BC023550 mRNA Translation: AAH23550.1
BC080537 mRNA Translation: AAH80537.1
AK002072 mRNA Translation: BAA92069.1 Different initiation.
CCDSiCCDS34796.1
RefSeqiNP_060831.2, NM_018361.3
UniGeneiHs.624002

Genome annotation databases

EnsembliENST00000285518; ENSP00000285518; ENSG00000155189
ENST00000646327; ENSP00000495562; ENSG00000284980
GeneIDi55326
KEGGihsa:55326
UCSCiuc003wqo.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF375789 mRNA Translation: AAK54809.1
AL136587 mRNA Translation: CAB66522.1
BC023550 mRNA Translation: AAH23550.1
BC080537 mRNA Translation: AAH80537.1
AK002072 mRNA Translation: BAA92069.1 Different initiation.
CCDSiCCDS34796.1
RefSeqiNP_060831.2, NM_018361.3
UniGeneiHs.624002

3D structure databases

ProteinModelPortaliQ9NUQ2
SMRiQ9NUQ2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120607, 11 interactors
IntActiQ9NUQ2, 6 interactors
MINTiQ9NUQ2
STRINGi9606.ENSP00000285518

Chemistry databases

SwissLipidsiSLP:000000822

PTM databases

iPTMnetiQ9NUQ2
PhosphoSitePlusiQ9NUQ2
SwissPalmiQ9NUQ2

Polymorphism and mutation databases

BioMutaiAGPAT5
DMDMi30923427

Proteomic databases

EPDiQ9NUQ2
MaxQBiQ9NUQ2
PaxDbiQ9NUQ2
PeptideAtlasiQ9NUQ2
PRIDEiQ9NUQ2
ProteomicsDBi82706

Protocols and materials databases

DNASUi55326
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000285518; ENSP00000285518; ENSG00000155189
ENST00000646327; ENSP00000495562; ENSG00000284980
GeneIDi55326
KEGGihsa:55326
UCSCiuc003wqo.4 human

Organism-specific databases

CTDi55326
DisGeNETi55326
EuPathDBiHostDB:ENSG00000155189.11
GeneCardsiAGPAT5
HGNCiHGNC:20886 AGPAT5
HPAiHPA010644
HPA010950
MIMi614796 gene
neXtProtiNX_Q9NUQ2
OpenTargetsiENSG00000155189
PharmGKBiPA134952751
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00890000139404
HOGENOMiHOG000007801
HOVERGENiHBG039632
InParanoidiQ9NUQ2
KOiK19007
OMAiDWIIADM
OrthoDBiEOG091G0C4Z
PhylomeDBiQ9NUQ2
TreeFamiTF314346

Enzyme and pathway databases

UniPathwayi
UPA00557;UER00613

BioCyciMetaCyc:HS08034-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA

Miscellaneous databases

ChiTaRSiAGPAT5 human
GeneWikiiAGPAT5
GenomeRNAii55326
PROiPR:Q9NUQ2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000155189 Expressed in 231 organ(s), highest expression level in placenta
CleanExiHS_AGPAT5
ExpressionAtlasiQ9NUQ2 baseline and differential
GenevisibleiQ9NUQ2 HS

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCE_HUMAN
AccessioniPrimary (citable) accession number: Q9NUQ2
Secondary accession number(s): Q8IZ47, Q9BQG4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 16, 2003
Last modified: October 10, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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