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Protein

Acetyl-coenzyme A synthetase 2-like, mitochondrial

Gene

ACSS1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO2.By similarity1 Publication

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.

Activity regulationi

Inhibited by acetylation at Lys-642 and activated by deacetylation.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei341Coenzyme ABy similarity1
Binding sitei533ATPBy similarity1
Binding sitei548ATPBy similarity1
Binding sitei556Coenzyme A; via carbonyl oxygenBy similarity1
Binding sitei559ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi417 – 419ATPBy similarity3
Nucleotide bindingi441 – 446ATPBy similarity6

GO - Molecular functioni

  • acetate-CoA ligase activity Source: UniProtKB
  • AMP binding Source: InterPro
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.1 2681
ReactomeiR-HSA-71384 Ethanol oxidation

Chemistry databases

SwissLipidsiSLP:000000448

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetase 2-like, mitochondrial (EC:6.2.1.1)
Alternative name(s):
Acetate--CoA ligase 2
Acetyl-CoA synthetase 2
Short name:
AceCS2
Acyl-CoA synthetase short-chain family member 1
Gene namesi
Name:ACSS1
Synonyms:ACAS2L, KIAA1846
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000154930.14
HGNCiHGNC:16091 ACSS1
MIMi614355 gene
neXtProtiNX_Q9NUB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi642K → Q: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi84532
OpenTargetsiENSG00000154930
PharmGKBiPA24430

Chemistry databases

DrugBankiDB00131 Adenosine monophosphate
DB00171 Adenosine triphosphate

Polymorphism and mutation databases

BioMutaiACSS1
DMDMi30172968

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37Mitochondrion1 PublicationAdd BLAST37
ChainiPRO_000000059638 – 689Acetyl-coenzyme A synthetase 2-like, mitochondrialAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei396N6-acetyllysineCombined sources1
Modified residuei642N6-acetyllysine1 Publication1

Post-translational modificationi

Reversibly acetylated on Lys-642. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9NUB1
MaxQBiQ9NUB1
PaxDbiQ9NUB1
PeptideAtlasiQ9NUB1
PRIDEiQ9NUB1
ProteomicsDBi82660
82661 [Q9NUB1-2]

PTM databases

iPTMnetiQ9NUB1
PhosphoSitePlusiQ9NUB1

Expressioni

Gene expression databases

BgeeiENSG00000154930 Expressed in 188 organ(s), highest expression level in placenta
CleanExiHS_ACSS1
ExpressionAtlasiQ9NUB1 baseline and differential
GenevisibleiQ9NUB1 HS

Organism-specific databases

HPAiHPA041014
HPA043228

Interactioni

Subunit structurei

Interacts with SIRT3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VAC14Q08AM65EBI-10313831,EBI-2107455

Protein-protein interaction databases

BioGridi124122, 8 interactors
DIPiDIP-61208N
IntActiQ9NUB1, 5 interactors
STRINGi9606.ENSP00000316924

Structurei

3D structure databases

ProteinModelPortaliQ9NUB1
SMRiQ9NUB1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NUB1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 227Coenzyme A bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi45 – 53Poly-Ala9

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1175 Eukaryota
COG0365 LUCA
GeneTreeiENSGT00760000119178
HOVERGENiHBG014401
InParanoidiQ9NUB1
KOiK01895
OMAiVNCLDQH
OrthoDBiEOG091G039C
PhylomeDBiQ9NUB1
TreeFamiTF354241

Family and domain databases

CDDicd05966 ACS, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NUB1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAARTLGRGV GRLLGSLRGL SGQPARPPCG VSAPRRAASG PSGSAPAVAA
60 70 80 90 100
AAAQPGSYPA LSAQAAREPA AFWGPLARDT LVWDTPYHTV WDCDFSTGKI
110 120 130 140 150
GWFLGGQLNV SVNCLDQHVR KSPESVALIW ERDEPGTEVR ITYRELLETT
160 170 180 190 200
CRLANTLKRH GVHRGDRVAI YMPVSPLAVA AMLACARIGA VHTVIFAGFS
210 220 230 240 250
AESLAGRIND AKCKVVITFN QGLRGGRVVE LKKIVDEAVK HCPTVQHVLV
260 270 280 290 300
AHRTDNKVHM GDLDVPLEQE MAKEDPVCAP ESMGSEDMLF MLYTSGSTGM
310 320 330 340 350
PKGIVHTQAG YLLYAALTHK LVFDHQPGDI FGCVADIGWI TGHSYVVYGP
360 370 380 390 400
LCNGATSVLF ESTPVYPNAG RYWETVERLK INQFYGAPTA VRLLLKYGDA
410 420 430 440 450
WVKKYDRSSL RTLGSVGEPI NCEAWEWLHR VVGDSRCTLV DTWWQTETGG
460 470 480 490 500
ICIAPRPSEE GAEILPAMAM RPFFGIVPVL MDEKGSVVEG SNVSGALCIS
510 520 530 540 550
QAWPGMARTI YGDHQRFVDA YFKAYPGYYF TGDGAYRTEG GYYQITGRMD
560 570 580 590 600
DVINISGHRL GTAEIEDAIA DHPAVPESAV IGYPHDIKGE AAFAFIVVKD
610 620 630 640 650
SAGDSDVVVQ ELKSMVATKI AKYAVPDEIL VVKRLPKTRS GKVMRRLLRK
660 670 680
IITSEAQELG DTTTLEDPSI IAEILSVYQK CKDKQAAAK
Length:689
Mass (Da):74,857
Last modified:April 23, 2003 - v2
Checksum:i66E84E39302AD08B
GO
Isoform 2 (identifier: Q9NUB1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     446-447: Missing.

Note: No experimental confirmation available.
Show »
Length:687
Mass (Da):74,627
Checksum:i99888770D7E10BA4
GO
Isoform 3 (identifier: Q9NUB1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MAARTLGRGVGRLLGSLRGLSG → MRRRKERQPWDRFHFLHFAPHG
     23-143: Missing.

Note: No experimental confirmation available.
Show »
Length:568
Mass (Da):62,722
Checksum:i061B9CF91C387B3F
GO
Isoform 4 (identifier: Q9NUB1-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     556-575: SGHRLGTAEIEDAIADHPAV → LQIVGFFREAIRNSGDLLEH
     576-689: Missing.

Note: No experimental confirmation available.
Show »
Length:575
Mass (Da):62,688
Checksum:i4D419E89EB8DF015
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5TF43Q5TF43_HUMAN
Acetyl-coenzyme A synthetase 2-like...
ACSS1
153Annotation score:

Sequence cautioni

The sequence BAB55390 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC03853 differs from that shown. Sequencing errors.Curated
The sequence BAC03853 differs from that shown. Reason: Frameshift at position 250.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233K → I in BAG54524 (PubMed:14702039).Curated1
Sequence conflicti277V → M in AAH39261 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048184488V → M2 PublicationsCorresponds to variant dbSNP:rs6050249Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0446931 – 22MAART…RGLSG → MRRRKERQPWDRFHFLHFAP HG in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_04469423 – 143Missing in isoform 3. 1 PublicationAdd BLAST121
Alternative sequenceiVSP_007249446 – 447Missing in isoform 2. 1 Publication2
Alternative sequenceiVSP_045546556 – 575SGHRL…DHPAV → LQIVGFFREAIRNSGDLLEH in isoform 4. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_045547576 – 689Missing in isoform 4. 1 PublicationAdd BLAST114

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027817 mRNA Translation: BAB55390.1 Different initiation.
AK092295 mRNA Translation: BAC03853.1 Sequence problems.
AK127566 mRNA Translation: BAG54524.1
AK296306 mRNA Translation: BAG59005.1
AL035661 Genomic DNA No translation available.
AL080312 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10106.1
CH471133 Genomic DNA Translation: EAX10109.1
BC039261 mRNA Translation: AAH39261.1
BC044588 mRNA Translation: AAH44588.1
AB058749 mRNA Translation: BAB47475.1
CCDSiCCDS13167.1 [Q9NUB1-1]
CCDS58764.1 [Q9NUB1-3]
CCDS58765.1 [Q9NUB1-4]
RefSeqiNP_001239604.1, NM_001252675.1 [Q9NUB1-2]
NP_001239605.1, NM_001252676.1 [Q9NUB1-3]
NP_001239606.1, NM_001252677.1 [Q9NUB1-4]
NP_115890.2, NM_032501.3 [Q9NUB1-1]
UniGeneiHs.529353

Genome annotation databases

EnsembliENST00000323482; ENSP00000316924; ENSG00000154930 [Q9NUB1-1]
ENST00000432802; ENSP00000388793; ENSG00000154930 [Q9NUB1-4]
ENST00000537502; ENSP00000439304; ENSG00000154930 [Q9NUB1-3]
GeneIDi84532
KEGGihsa:84532
UCSCiuc002wub.4 human [Q9NUB1-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027817 mRNA Translation: BAB55390.1 Different initiation.
AK092295 mRNA Translation: BAC03853.1 Sequence problems.
AK127566 mRNA Translation: BAG54524.1
AK296306 mRNA Translation: BAG59005.1
AL035661 Genomic DNA No translation available.
AL080312 Genomic DNA No translation available.
CH471133 Genomic DNA Translation: EAX10106.1
CH471133 Genomic DNA Translation: EAX10109.1
BC039261 mRNA Translation: AAH39261.1
BC044588 mRNA Translation: AAH44588.1
AB058749 mRNA Translation: BAB47475.1
CCDSiCCDS13167.1 [Q9NUB1-1]
CCDS58764.1 [Q9NUB1-3]
CCDS58765.1 [Q9NUB1-4]
RefSeqiNP_001239604.1, NM_001252675.1 [Q9NUB1-2]
NP_001239605.1, NM_001252676.1 [Q9NUB1-3]
NP_001239606.1, NM_001252677.1 [Q9NUB1-4]
NP_115890.2, NM_032501.3 [Q9NUB1-1]
UniGeneiHs.529353

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3GLRX-ray1.80B638-649[»]
3GLTX-ray2.10B638-649[»]
3GLUX-ray2.50B638-649[»]
4BVEX-ray2.05B638-647[»]
4BVFX-ray2.70B638-647[»]
4BVGX-ray2.50B638-647[»]
4C78X-ray2.00C638-647[»]
5Y4HX-ray2.60B638-649[»]
5YTKX-ray2.70G/J/K/L638-645[»]
ProteinModelPortaliQ9NUB1
SMRiQ9NUB1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi124122, 8 interactors
DIPiDIP-61208N
IntActiQ9NUB1, 5 interactors
STRINGi9606.ENSP00000316924

Chemistry databases

DrugBankiDB00131 Adenosine monophosphate
DB00171 Adenosine triphosphate
SwissLipidsiSLP:000000448

PTM databases

iPTMnetiQ9NUB1
PhosphoSitePlusiQ9NUB1

Polymorphism and mutation databases

BioMutaiACSS1
DMDMi30172968

Proteomic databases

EPDiQ9NUB1
MaxQBiQ9NUB1
PaxDbiQ9NUB1
PeptideAtlasiQ9NUB1
PRIDEiQ9NUB1
ProteomicsDBi82660
82661 [Q9NUB1-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000323482; ENSP00000316924; ENSG00000154930 [Q9NUB1-1]
ENST00000432802; ENSP00000388793; ENSG00000154930 [Q9NUB1-4]
ENST00000537502; ENSP00000439304; ENSG00000154930 [Q9NUB1-3]
GeneIDi84532
KEGGihsa:84532
UCSCiuc002wub.4 human [Q9NUB1-1]

Organism-specific databases

CTDi84532
DisGeNETi84532
EuPathDBiHostDB:ENSG00000154930.14
GeneCardsiACSS1
H-InvDBiHIX0015699
HGNCiHGNC:16091 ACSS1
HPAiHPA041014
HPA043228
MIMi614355 gene
neXtProtiNX_Q9NUB1
OpenTargetsiENSG00000154930
PharmGKBiPA24430
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1175 Eukaryota
COG0365 LUCA
GeneTreeiENSGT00760000119178
HOVERGENiHBG014401
InParanoidiQ9NUB1
KOiK01895
OMAiVNCLDQH
OrthoDBiEOG091G039C
PhylomeDBiQ9NUB1
TreeFamiTF354241

Enzyme and pathway databases

BRENDAi6.2.1.1 2681
ReactomeiR-HSA-71384 Ethanol oxidation

Miscellaneous databases

ChiTaRSiACSS1 human
EvolutionaryTraceiQ9NUB1
GenomeRNAii84532
PROiPR:Q9NUB1
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000154930 Expressed in 188 organ(s), highest expression level in placenta
CleanExiHS_ACSS1
ExpressionAtlasiQ9NUB1 baseline and differential
GenevisibleiQ9NUB1 HS

Family and domain databases

CDDicd05966 ACS, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACS2L_HUMAN
AccessioniPrimary (citable) accession number: Q9NUB1
Secondary accession number(s): B3KXL2
, B4DJZ3, D3DW48, F5H6F4, F8W7Y1, Q5TF42, Q8IV99, Q8N234, Q96JI1, Q96JX6, Q9NU28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: April 23, 2003
Last modified: October 10, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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