RNF146

Functionⁱ

E3 ubiquitin-protein ligase that specifically binds poly-ADP-ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor-mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation (By similarity). Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair.By similarity5 Publications

Miscellaneous

Was named dactylidin after the Greek term 'daktylidi' for ring, 'the thing around the finger' (PubMed:15813938). Was named Iduna after the Norse goddess of protection and eternal youth (PubMed:21602803).2 Publications

Catalytic activityⁱ

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	108	iso-ADP-ribose adenine ring group1 Publication	1
Binding siteⁱ	111	iso-ADP-ribose 5'-phosphate group1 Publication	1
Binding siteⁱ	115	iso-ADP-ribose 5'-phosphate group1 Publication	1
Binding siteⁱ	145	iso-ADP-ribose 1'-phosphate group1 Publication	1
Binding siteⁱ	154	iso-ADP-ribose adenine ring group1 Publication	1
Binding siteⁱ	164	iso-ADP-ribose 1'-phosphate group1 Publication	1
Binding siteⁱ	176	iso-ADP-ribose 5'-phosphate group1 Publication	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	37 – 75	RING-typePROSITE-ProRule annotationAdd BLAST		39

GO - Molecular functionⁱ

poly-ADP-D-ribose binding
Source: UniProtKB
Inferred from direct assayⁱ
- 21478859
ubiquitin protein ligase activity Source: Reactome
ubiquitin-protein transferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 21478859
zinc ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

positive regulation of canonical Wnt signaling pathway
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21478859
protein autoubiquitination
Source: UniProtKB
Inferred from direct assayⁱ
- 21478859
protein K48-linked ubiquitination
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21478859
ubiquitin-dependent protein catabolic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 21478859
Wnt signaling pathway Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway, Wnt signaling pathway
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-4641257 Degradation of AXIN R-HSA-5689880 Ub-specific processing proteases R-HSA-8948751 Regulation of PTEN stability and activity
UniPathwayⁱ	UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: E3 ubiquitin-protein ligase RNF146 (EC:2.3.2.27) Alternative name(s): Dactylidin Iduna RING finger protein 146 RING-type E3 ubiquitin transferase RNF146Curated
Gene namesⁱ	Name:RNF146
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 6

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000118518.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:21336 RNF146
MIMⁱ	612137 gene
neXtProtⁱ	NX_Q9NTX7

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Defects in RNF146 are a cause of susceptibility to breast cancer.

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	54	H → A: Partially suppression of WNT3A signaling and stabilization of AXIN1, TNKS and TNKS2 with or without WNT3A induction. No effect on TNKS1-binding. 1 Publication	1
Mutagenesisⁱ	106	W → A: No effect on Wnt signaling pathway. 1 Publication	1
Mutagenesisⁱ	108	Y → A: Loss of iso-ADP-ribose-binding. 1 Publication	1
Mutagenesisⁱ	111	R → A: Minor effect on iso-ADP-ribose-binding. 1 Publication	1
Mutagenesisⁱ	115	W → A: Strong decrease in iso-ADP-ribose-binding affinity. 1 Publication	1
Mutagenesisⁱ	145	Y → A: Loss of iso-ADP-ribose-binding. 1 Publication	1
Mutagenesisⁱ	154	Q → A: Loss of iso-ADP-ribose-binding affinity. 1 Publication	1
Mutagenesisⁱ	163	R → A: Abolishes the ability to recognize and bind PARsylated proteins. 1 Publication	1
Mutagenesisⁱ	164	R → A: Loss of iso-ADP-ribose-binding. 1 Publication	1
Mutagenesisⁱ	176	K → A: Minor effect on iso-ADP-ribose-binding. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	81847
Open Targets More...OpenTargetsⁱ	ENSG00000118518
PharmGKBⁱ	PA134910489

Polymorphism and mutation databases

BioMutaⁱ	RNF146
DMDMⁱ	60390653

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000056107	1 – 359	E3 ubiquitin-protein ligase RNF146Add BLAST		359

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	85	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linkⁱ	95	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linkⁱ	131	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linkⁱ	176	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residueⁱ	290	PhosphoserineBy similarity	1
Modified residueⁱ	294	PhosphoserineBy similarity	1

Post-translational modificationⁱ

Ubiquitinated; autoubiquitinated. Polyubiquitinated in the presence of UBE2D1, UBE2D2 and UBE2D3. Multimonoubiquitinated in the presence of UBE2E1. Not ubiquitinated in the presence of UBE2H, CDC34, UBE2L3, UBE2L6, nor UBE2C. In the absence of PAR, autoubiquitination occurs on Lys-85, Lys-95 and Lys-176 via 'Lys-11' and 'Lys-48' ubiquitin linkages. In the presence of PAR, Lys-131 and Lys-176 are ubiquitinated via 'Lys-6', 'Lys-33' and 'Lys-48' ubiquitin linkages. Autoubiquitination is enhanced upon PAR-binding.3 Publications

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9NTX7
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NTX7
PaxDbⁱ	Q9NTX7
PeptideAtlas More...PeptideAtlasⁱ	Q9NTX7
PRIDEⁱ	Q9NTX7
ProteomicsDBⁱ	82642 82643 [Q9NTX7-2]

PTM databases

iPTMnetⁱ	Q9NTX7
PhosphoSitePlusⁱ	Q9NTX7

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed. Up-regulated in brains from patients with Alzheimer disease.

Gene expression databases

Bgeeⁱ	ENSG00000118518 Expressed in 217 organ(s), highest expression level in cerebellar vermis
CleanExⁱ	HS_RNF146
ExpressionAtlasⁱ	Q9NTX7 baseline and differential
Genevisibleⁱ	Q9NTX7 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA027158 HPA027209

HPAⁱ

HPA027158
HPA027209

Interactionⁱ

Subunit structureⁱ

Can form homooligomers. Interacts with PARsylated AXIN1, AXIN2, BLZF1, CASC3, HIST1H1C, IPO7, LIG3, NCL, PARP1, XRCC1, XRCC5 and XRCC6. Interacts with DDB1, DHX15, IQGAP1, LRPPRC, PARP2, PRKDC, RUVBL2, TNKS1 and TNKS2. Binding often leads to interactor ubiquitination, in the presence of the appropriate E1 and E2 enzymes, and proteasomal degradation.4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CCDC155	Q8N6L0	4	EBI-11750630,EBI-749265
TNKS	O95271	4	EBI-722397,EBI-1105254
TRAF2	Q12933	3	EBI-722397,EBI-355744
TUBGCP4	Q9UGJ1	3	EBI-722397,EBI-1052544

Protein-protein interaction databases

BioGridⁱ	123598, 39 interactors
Database of interacting proteins More...DIPⁱ	DIP-52730N
IntActⁱ	Q9NTX7, 11 interactors
STRINGⁱ	9606.ENSP00000357297

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	30 – 33	Combined sources	4
Beta strandⁱ	38 – 43	Combined sources	6
Beta strandⁱ	45 – 50	Combined sources	6
Turnⁱ	51 – 53	Combined sources	3
Beta strandⁱ	54 – 57	Combined sources	4
Helixⁱ	58 – 63	Combined sources	6
Beta strandⁱ	68 – 70	Combined sources	3
Beta strandⁱ	72 – 74	Combined sources	3
Helixⁱ	80 – 83	Combined sources	4
Beta strandⁱ	104 – 109	Combined sources	6
Beta strandⁱ	111 – 116	Combined sources	6
Helixⁱ	119 – 130	Combined sources	12
Beta strandⁱ	134 – 140	Combined sources	7
Beta strandⁱ	143 – 148	Combined sources	6
Turnⁱ	149 – 152	Combined sources	4
Beta strandⁱ	153 – 159	Combined sources	7
Beta strandⁱ	164 – 173	Combined sources	10

Show more details

3D structure databases

ProteinModelPortalⁱ	Q9NTX7
SMRⁱ	Q9NTX7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9NTX7

EvolutionaryTraceⁱ

Q9NTX7

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	92 – 168	WWEPROSITE-ProRule annotationAdd BLAST		77

Domainⁱ

The WWE domain mediates non-covalent PAR-binding.1 Publication

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	37 – 75	RING-typePROSITE-ProRule annotationAdd BLAST		39

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG0824 Eukaryota ENOG410ZTFC LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000000358
HOVERGENⁱ	HBG057514
InParanoidⁱ	Q9NTX7
KEGG Orthology (KO) More...KOⁱ	K15700
OMAⁱ	RMAGCGE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0Y4Y
PhylomeDBⁱ	Q9NTX7
TreeFamⁱ	TF318925

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit 3.30.720.50, 1 hit
InterProⁱ	View protein in InterPro IPR033509 RNF146 IPR004170 WWE-dom IPR018123 WWE-dom_subgr IPR037197 WWE_dom_sf IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR017907 Znf_RING_CS
PANTHERⁱ	PTHR13417:SF2 PTHR13417:SF2, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02825 WWE, 1 hit
SMARTⁱ	View protein in SMART SM00184 RING, 1 hit SM00678 WWE, 1 hit
SUPFAMⁱ	SSF117839 SSF117839, 1 hit
PROSITEⁱ	View protein in PROSITE PS50918 WWE, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9NTX7-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMAGCGEIDH SINMLPTNRK ANESCSNTAP SLTVPECAIC LQTCVHPVSL 
        60         70         80         90        100
PCKHVFCYLC VKGASWLGKR CALCRQEIPE DFLDKPTLLS PEELKAASRG 
       110        120        130        140        150
NGEYAWYYEG RNGWWQYDER TSRELEDAFS KGKKNTEMLI AGFLYVADLE 
       160        170        180        190        200
NMVQYRRNEH GRRRKIKRDI IDIPKKGVAG LRLDCDANTV NLARESSADG 
       210        220        230        240        250
ADSVSAQSGA SVQPLVSSVR PLTSVDGQLT SPATPSPDAS TSLEDSFAHL 
       260        270        280        290        300
QLSGDNTAER SHRGEGEEDH ESPSSGRVPA PDTSIEETES DASSDSEDVS 
       310        320        330        340        350
AVVAQHSLTQ QRLLVSNANQ TVPDRSDRSG TDRSVAGGGT VSVSVRSRRP 

DGQCTVTEV

Length:359

Mass (Da):38,950

Last modified:October 1, 2000 - v1

Checksum:ⁱ7337C410EDA30A7E

GO

Isoform 2 (identifier: Q9NTX7-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-1: Missing.

« Hide

        10         20         30         40         50
MAGCGEIDHS INMLPTNRKA NESCSNTAPS LTVPECAICL QTCVHPVSLP 
        60         70         80         90        100
CKHVFCYLCV KGASWLGKRC ALCRQEIPED FLDKPTLLSP EELKAASRGN 
       110        120        130        140        150
GEYAWYYEGR NGWWQYDERT SRELEDAFSK GKKNTEMLIA GFLYVADLEN 
       160        170        180        190        200
MVQYRRNEHG RRRKIKRDII DIPKKGVAGL RLDCDANTVN LARESSADGA 
       210        220        230        240        250
DSVSAQSGAS VQPLVSSVRP LTSVDGQLTS PATPSPDAST SLEDSFAHLQ 
       260        270        280        290        300
LSGDNTAERS HRGEGEEDHE SPSSGRVPAP DTSIEETESD ASSDSEDVSA 
       310        320        330        340        350
VVAQHSLTQQ RLLVSNANQT VPDRSDRSGT DRSVAGGGTV SVSVRSRRPD 

GQCTVTEV

Show »

Length:358

Mass (Da):38,819

Checksum:ⁱE46AA6522DA8D48B

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

V9GYY4	V9GYY4_HUMAN	E3 ubiquitin-protein ligase RNF146 E3 ubiquitin-protein ligase RNF146	RNF146	40	Annotation score:
V9GZ53	V9GZ53_HUMAN	E3 ubiquitin-protein ligase RNF146 E3 ubiquitin-protein ligase RNF146	RNF146	14	Annotation score:
V9GYK9	V9GYK9_HUMAN	E3 ubiquitin-protein ligase RNF146 E3 ubiquitin-protein ligase RNF146	RNF146	42	Annotation score:
A0A0G2JLL7	A0A0G2JLL7_HUMAN	E3 ubiquitin-protein ligase RNF146 E3 ubiquitin-protein ligase RNF146	RNF146	9	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	69	K → R in BAB55359 (PubMed:14702039).Curated	1
Sequence conflictⁱ	74	C → R in BAB55108 (PubMed:14702039).Curated	1
Sequence conflictⁱ	100	G → E in CAG38545 (Ref. 4) Curated	1
Sequence conflictⁱ	166	I → V in CAG38545 (Ref. 4) Curated	1
Sequence conflictⁱ	229	L → S in BAB55108 (PubMed:14702039).Curated	1
Sequence conflictⁱ	329	S → L in BAB55108 (PubMed:14702039).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_065249	25	C → R. Corresponds to variant dbSNP:rs10081141Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_012968	1	Missing in isoform 2. 5 Publications		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ315122 mRNA Translation: CAC85986.1 AL136829 mRNA Translation: CAB66763.1 AK027558 mRNA Translation: BAB55196.1 AK027436 mRNA Translation: BAB55108.1 AK027776 mRNA Translation: BAB55359.1 CR533514 mRNA Translation: CAG38545.1 AL109939 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48109.1 CH471051 Genomic DNA Translation: EAW48111.1 BC008235 mRNA Translation: AAH08235.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS5136.1 [Q9NTX7-2] CCDS56449.1 [Q9NTX7-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001229773.1, NM_001242844.1 [Q9NTX7-2] NP_001229774.1, NM_001242845.1 [Q9NTX7-2] NP_001229775.1, NM_001242846.1 [Q9NTX7-2] NP_001229776.1, NM_001242847.1 [Q9NTX7-2] NP_001229777.1, NM_001242848.1 [Q9NTX7-2] NP_001229778.1, NM_001242849.1 [Q9NTX7-1] NP_001229779.1, NM_001242850.1 [Q9NTX7-1] NP_001229780.1, NM_001242851.1 [Q9NTX7-1] NP_001229781.1, NM_001242852.1 [Q9NTX7-2] NP_112225.2, NM_030963.3 [Q9NTX7-2] XP_006715634.1, XM_006715571.3 [Q9NTX7-2] XP_011534463.1, XM_011536161.2 [Q9NTX7-2] XP_011534464.1, XM_011536162.2 [Q9NTX7-2] XP_011534465.1, XM_011536163.2 [Q9NTX7-2] XP_011534466.1, XM_011536164.2 [Q9NTX7-2] XP_016866825.1, XM_017011336.1 [Q9NTX7-1] XP_016866826.1, XM_017011337.1 XP_016866827.1, XM_017011338.1 [Q9NTX7-2] XP_016866828.1, XM_017011339.1 [Q9NTX7-2] XP_016866829.1, XM_017011340.1 [Q9NTX7-2] XP_016866830.1, XM_017011341.1 [Q9NTX7-2] XP_016866831.1, XM_017011342.1 [Q9NTX7-2] XP_016866832.1, XM_017011343.1 [Q9NTX7-2]
UniGeneⁱ	Hs.267120

Genome annotation databases

Ensemblⁱ	ENST00000309649; ENSP00000309365; ENSG00000118518 [Q9NTX7-2] ENST00000368314; ENSP00000357297; ENSG00000118518 [Q9NTX7-1] ENST00000608991; ENSP00000477168; ENSG00000118518 [Q9NTX7-2] ENST00000610153; ENSP00000476814; ENSG00000118518 [Q9NTX7-1] ENST00000616343; ENSP00000479890; ENSG00000118518 [Q9NTX7-1]
GeneIDⁱ	81847
KEGGⁱ	hsa:81847
UCSC genome browser More...UCSCⁱ	uc003qav.4 human [Q9NTX7-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NTX7	RNF146 isoform 2		PANTR		359	UniRef100_Q9NTX7
Uncharacterized protein		PANPA		359
Isoform 2 of E3 ubiquitin-protein ligase RNF146		HUMAN		358
RNF146 isoform 4		PANTR		358

Protein	Similar proteins		Species	Score	Length	Source
Q9NTX7	E3 ubiquitin-protein ligase RNF146		MACFA		360	UniRef90_Q9NTX7
E3 ubiquitin-protein ligase RNF146	)	MOUSE		359
E3 ubiquitin-protein ligase RNF146		AILME		359
E3 ubiquitin-protein ligase RNF146		PONAB		358
E3 ubiquitin-protein ligase RNF146		RAT		352
+107

Protein	Similar proteins		Species	Score	Length	Source
Q9NTX7	E3 ubiquitin-protein ligase RNF146		MACFA		360	UniRef50_Q9NTX7
E3 ubiquitin-protein ligase RNF146	)	MOUSE		359
E3 ubiquitin-protein ligase RNF146		AILME		359
E3 ubiquitin-protein ligase RNF146		PONAB		358
E3 ubiquitin-protein ligase RNF146		RAT		352
+393

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ315122 mRNA Translation: CAC85986.1 AL136829 mRNA Translation: CAB66763.1 AK027558 mRNA Translation: BAB55196.1 AK027436 mRNA Translation: BAB55108.1 AK027776 mRNA Translation: BAB55359.1 CR533514 mRNA Translation: CAG38545.1 AL109939 Genomic DNA No translation available. CH471051 Genomic DNA Translation: EAW48109.1 CH471051 Genomic DNA Translation: EAW48111.1 BC008235 mRNA Translation: AAH08235.1
CCDSⁱ	CCDS5136.1 [Q9NTX7-2] CCDS56449.1 [Q9NTX7-1]
RefSeqⁱ	NP_001229773.1, NM_001242844.1 [Q9NTX7-2] NP_001229774.1, NM_001242845.1 [Q9NTX7-2] NP_001229775.1, NM_001242846.1 [Q9NTX7-2] NP_001229776.1, NM_001242847.1 [Q9NTX7-2] NP_001229777.1, NM_001242848.1 [Q9NTX7-2] NP_001229778.1, NM_001242849.1 [Q9NTX7-1] NP_001229779.1, NM_001242850.1 [Q9NTX7-1] NP_001229780.1, NM_001242851.1 [Q9NTX7-1] NP_001229781.1, NM_001242852.1 [Q9NTX7-2] NP_112225.2, NM_030963.3 [Q9NTX7-2] XP_006715634.1, XM_006715571.3 [Q9NTX7-2] XP_011534463.1, XM_011536161.2 [Q9NTX7-2] XP_011534464.1, XM_011536162.2 [Q9NTX7-2] XP_011534465.1, XM_011536163.2 [Q9NTX7-2] XP_011534466.1, XM_011536164.2 [Q9NTX7-2] XP_016866825.1, XM_017011336.1 [Q9NTX7-1] XP_016866826.1, XM_017011337.1 XP_016866827.1, XM_017011338.1 [Q9NTX7-2] XP_016866828.1, XM_017011339.1 [Q9NTX7-2] XP_016866829.1, XM_017011340.1 [Q9NTX7-2] XP_016866830.1, XM_017011341.1 [Q9NTX7-2] XP_016866831.1, XM_017011342.1 [Q9NTX7-2] XP_016866832.1, XM_017011343.1 [Q9NTX7-2]
UniGeneⁱ	Hs.267120

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2D8T	NMR	-	A	27-84	[»]
	3V3L	X-ray	1.65	A/B	100-184	[»]
	6CF6	X-ray	1.93	C/D	191-203	[»]
ProteinModelPortalⁱ	Q9NTX7
SMRⁱ	Q9NTX7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	123598, 39 interactors
DIPⁱ	DIP-52730N
IntActⁱ	Q9NTX7, 11 interactors
STRINGⁱ	9606.ENSP00000357297

PTM databases

iPTMnetⁱ	Q9NTX7
PhosphoSitePlusⁱ	Q9NTX7

Polymorphism and mutation databases

BioMutaⁱ	RNF146
DMDMⁱ	60390653

Proteomic databases

EPDⁱ	Q9NTX7
MaxQBⁱ	Q9NTX7
PaxDbⁱ	Q9NTX7
PeptideAtlasⁱ	Q9NTX7
PRIDEⁱ	Q9NTX7
ProteomicsDBⁱ	82642 82643 [Q9NTX7-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	81847
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000309649; ENSP00000309365; ENSG00000118518 [Q9NTX7-2] ENST00000368314; ENSP00000357297; ENSG00000118518 [Q9NTX7-1] ENST00000608991; ENSP00000477168; ENSG00000118518 [Q9NTX7-2] ENST00000610153; ENSP00000476814; ENSG00000118518 [Q9NTX7-1] ENST00000616343; ENSP00000479890; ENSG00000118518 [Q9NTX7-1]
GeneIDⁱ	81847
KEGGⁱ	hsa:81847
UCSCⁱ	uc003qav.4 human [Q9NTX7-1]

Organism-specific databases

CTDⁱ	81847
DisGeNETⁱ	81847
EuPathDBⁱ	HostDB:ENSG00000118518.15
GeneCardsⁱ	RNF146
HGNCⁱ	HGNC:21336 RNF146
HPAⁱ	HPA027158 HPA027209
MIMⁱ	612137 gene
neXtProtⁱ	NX_Q9NTX7
OpenTargetsⁱ	ENSG00000118518
PharmGKBⁱ	PA134910489
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0824 Eukaryota ENOG410ZTFC LUCA
GeneTreeⁱ	ENSGT00390000000358
HOVERGENⁱ	HBG057514
InParanoidⁱ	Q9NTX7
KOⁱ	K15700
OMAⁱ	RMAGCGE
OrthoDBⁱ	EOG091G0Y4Y
PhylomeDBⁱ	Q9NTX7
TreeFamⁱ	TF318925

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-4641257 Degradation of AXIN R-HSA-5689880 Ub-specific processing proteases R-HSA-8948751 Regulation of PTEN stability and activity

Miscellaneous databases

ChiTaRSⁱ	RNF146 human
EvolutionaryTraceⁱ	Q9NTX7
GeneWikiⁱ	RNF146
GenomeRNAiⁱ	81847
Protein Ontology More...PROⁱ	PR:Q9NTX7
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000118518 Expressed in 217 organ(s), highest expression level in cerebellar vermis
CleanExⁱ	HS_RNF146
ExpressionAtlasⁱ	Q9NTX7 baseline and differential
Genevisibleⁱ	Q9NTX7 HS

Family and domain databases

Gene3Dⁱ	3.30.40.10, 1 hit 3.30.720.50, 1 hit
InterProⁱ	View protein in InterPro IPR033509 RNF146 IPR004170 WWE-dom IPR018123 WWE-dom_subgr IPR037197 WWE_dom_sf IPR001841 Znf_RING IPR013083 Znf_RING/FYVE/PHD IPR017907 Znf_RING_CS
PANTHERⁱ	PTHR13417:SF2 PTHR13417:SF2, 1 hit
Pfamⁱ	View protein in Pfam PF02825 WWE, 1 hit
SMARTⁱ	View protein in SMART SM00184 RING, 1 hit SM00678 WWE, 1 hit
SUPFAMⁱ	SSF117839 SSF117839, 1 hit
PROSITEⁱ	View protein in PROSITE PS50918 WWE, 1 hit PS00518 ZF_RING_1, 1 hit PS50089 ZF_RING_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RN146_HUMAN
Accessionⁱ	Q9NTX7Primary (citable) accession number: Q9NTX7 Secondary accession number(s): E1P572 , Q6FIB2, Q7L8H4, Q96K03, Q96T06, Q9NTX6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
	Last sequence update:	October 1, 2000
	Last modified:	October 10, 2018
	This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM

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UniProtKB - Q9NTX7 (RN146_HUMAN)

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E3 ubiquitin-protein ligase RNF146

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

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Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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Cytosol

Nucleus

Plasma Membrane

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

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Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

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Zinc finger

Keywords - Domaini

Phylogenomic databases

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Experimental Info

Natural variant

Alternative sequence

Sequence databases

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Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ