UniProtKB - Q9NTX7 (RN146_HUMAN)
E3 ubiquitin-protein ligase RNF146
RNF146
Functioni
Miscellaneous
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 108 | iso-ADP-ribose adenine ring group1 Publication | 1 | |
Binding sitei | 111 | iso-ADP-ribose 5'-phosphate group1 Publication | 1 | |
Binding sitei | 115 | iso-ADP-ribose 5'-phosphate group1 Publication | 1 | |
Binding sitei | 145 | iso-ADP-ribose 1'-phosphate group1 Publication | 1 | |
Binding sitei | 154 | iso-ADP-ribose adenine ring group1 Publication | 1 | |
Binding sitei | 164 | iso-ADP-ribose 1'-phosphate group1 Publication | 1 | |
Binding sitei | 176 | iso-ADP-ribose 5'-phosphate group1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 37 – 75 | RING-typePROSITE-ProRule annotationAdd BLAST | 39 |
GO - Molecular functioni
- poly-ADP-D-ribose binding Source: UniProtKB
- ubiquitin protein ligase activity Source: Reactome
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- positive regulation of canonical Wnt signaling pathway Source: UniProtKB
- protein autoubiquitination Source: UniProtKB
- protein K48-linked ubiquitination Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway, Wnt signaling pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9NTX7 |
Reactomei | R-HSA-201681, TCF dependent signaling in response to WNT R-HSA-4641257, Degradation of AXIN R-HSA-5689880, Ub-specific processing proteases R-HSA-8948751, Regulation of PTEN stability and activity |
SIGNORi | Q9NTX7 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF146 (EC:2.3.2.27)Alternative name(s): Dactylidin Iduna RING finger protein 146 RING-type E3 ubiquitin transferase RNF146Curated |
Gene namesi | Name:RNF146 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000118518.15 |
HGNCi | HGNC:21336, RNF146 |
MIMi | 612137, gene |
neXtProti | NX_Q9NTX7 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Involvement in diseasei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 54 | H → A: Partially suppression of WNT3A signaling and stabilization of AXIN1, TNKS and TNKS2 with or without WNT3A induction. No effect on TNKS1-binding. 1 Publication | 1 | |
Mutagenesisi | 106 | W → A: No effect on Wnt signaling pathway. 1 Publication | 1 | |
Mutagenesisi | 108 | Y → A: Loss of iso-ADP-ribose-binding. 1 Publication | 1 | |
Mutagenesisi | 111 | R → A: Minor effect on iso-ADP-ribose-binding. 1 Publication | 1 | |
Mutagenesisi | 115 | W → A: Strong decrease in iso-ADP-ribose-binding affinity. 1 Publication | 1 | |
Mutagenesisi | 145 | Y → A: Loss of iso-ADP-ribose-binding. 1 Publication | 1 | |
Mutagenesisi | 154 | Q → A: Loss of iso-ADP-ribose-binding affinity. 1 Publication | 1 | |
Mutagenesisi | 163 | R → A: Abolishes the ability to recognize and bind PARsylated proteins. 1 Publication | 1 | |
Mutagenesisi | 164 | R → A: Loss of iso-ADP-ribose-binding. 1 Publication | 1 | |
Mutagenesisi | 176 | K → A: Minor effect on iso-ADP-ribose-binding. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 81847 |
OpenTargetsi | ENSG00000118518 |
PharmGKBi | PA134910489 |
Miscellaneous databases
Pharosi | Q9NTX7, Tbio |
Polymorphism and mutation databases
BioMutai | RNF146 |
DMDMi | 60390653 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056107 | 1 – 359 | E3 ubiquitin-protein ligase RNF146Add BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 85 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 95 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 131 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Cross-linki | 176 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication | ||
Modified residuei | 290 | PhosphoserineBy similarity | 1 | |
Modified residuei | 294 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q9NTX7 |
MassIVEi | Q9NTX7 |
MaxQBi | Q9NTX7 |
PaxDbi | Q9NTX7 |
PeptideAtlasi | Q9NTX7 |
PRIDEi | Q9NTX7 |
ProteomicsDBi | 82642 [Q9NTX7-1] 82643 [Q9NTX7-2] |
PTM databases
iPTMneti | Q9NTX7 |
PhosphoSitePlusi | Q9NTX7 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000118518, Expressed in cerebellar vermis and 230 other tissues |
ExpressionAtlasi | Q9NTX7, baseline and differential |
Genevisiblei | Q9NTX7, HS |
Organism-specific databases
HPAi | ENSG00000118518, Low tissue specificity |
Interactioni
Subunit structurei
Can form homooligomers.
Interacts with PARsylated AXIN1, AXIN2, BLZF1, CASC3, H1-2, IPO7, LIG3, NCL, PARP1, XRCC1, XRCC5 and XRCC6.
Interacts with DDB1, DHX15, IQGAP1, LRPPRC, PARP2, PRKDC, RUVBL2, TNKS1 and TNKS2. Binding often leads to interactor ubiquitination, in the presence of the appropriate E1 and E2 enzymes, and proteasomal degradation.
4 PublicationsBinary interactionsi
Hide detailsQ9NTX7
With | #Exp. | IntAct |
---|---|---|
KASH5 [Q8N6L0] | 3 | EBI-722397,EBI-749265 |
PARP1 [P09874] | 4 | EBI-722397,EBI-355676 |
TNKS [O95271] | 6 | EBI-722397,EBI-1105254 |
TRAF2 [Q12933] | 3 | EBI-722397,EBI-355744 |
TUBGCP4 [Q9UGJ1] | 3 | EBI-722397,EBI-1052544 |
Isoform 2 [Q9NTX7-2]
With | #Exp. | IntAct |
---|---|---|
KASH5 [Q8N6L0] | 7 | EBI-11750630,EBI-749265 |
NDUFAF3 [Q9BU61] | 3 | EBI-11750630,EBI-2114801 |
TRAF2 [Q12933] | 3 | EBI-11750630,EBI-355744 |
Protein-protein interaction databases
BioGRIDi | 123598, 59 interactors |
DIPi | DIP-52730N |
IntActi | Q9NTX7, 46 interactors |
STRINGi | 9606.ENSP00000357297 |
Miscellaneous databases
RNActi | Q9NTX7, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9NTX7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NTX7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 92 – 168 | WWEPROSITE-ProRule annotationAdd BLAST | 77 |
Domaini
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 37 – 75 | RING-typePROSITE-ProRule annotationAdd BLAST | 39 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0824, Eukaryota |
GeneTreei | ENSGT00390000000358 |
HOGENOMi | CLU_067425_0_0_1 |
InParanoidi | Q9NTX7 |
OMAi | AHLQING |
OrthoDBi | 1469576at2759 |
PhylomeDBi | Q9NTX7 |
TreeFami | TF318925 |
Family and domain databases
CDDi | cd16546, RING-HC_RNF146, 1 hit |
Gene3Di | 3.30.40.10, 1 hit 3.30.720.50, 1 hit |
InterProi | View protein in InterPro IPR044110, RING-HC_RNF146 IPR033509, RNF146 IPR004170, WWE-dom IPR018123, WWE-dom_subgr IPR037197, WWE_dom_sf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR13417:SF2, PTHR13417:SF2, 1 hit |
Pfami | View protein in Pfam PF02825, WWE, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit SM00678, WWE, 1 hit |
SUPFAMi | SSF117839, SSF117839, 1 hit |
PROSITEi | View protein in PROSITE PS50918, WWE, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMAGCGEIDH SINMLPTNRK ANESCSNTAP SLTVPECAIC LQTCVHPVSL
60 70 80 90 100
PCKHVFCYLC VKGASWLGKR CALCRQEIPE DFLDKPTLLS PEELKAASRG
110 120 130 140 150
NGEYAWYYEG RNGWWQYDER TSRELEDAFS KGKKNTEMLI AGFLYVADLE
160 170 180 190 200
NMVQYRRNEH GRRRKIKRDI IDIPKKGVAG LRLDCDANTV NLARESSADG
210 220 230 240 250
ADSVSAQSGA SVQPLVSSVR PLTSVDGQLT SPATPSPDAS TSLEDSFAHL
260 270 280 290 300
QLSGDNTAER SHRGEGEEDH ESPSSGRVPA PDTSIEETES DASSDSEDVS
310 320 330 340 350
AVVAQHSLTQ QRLLVSNANQ TVPDRSDRSG TDRSVAGGGT VSVSVRSRRP
DGQCTVTEV
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9GYY4 | V9GYY4_HUMAN | E3 ubiquitin-protein ligase RNF146 | RNF146 | 40 | Annotation score: | ||
V9GZ53 | V9GZ53_HUMAN | E3 ubiquitin-protein ligase RNF146 | RNF146 | 14 | Annotation score: | ||
V9GYK9 | V9GYK9_HUMAN | E3 ubiquitin-protein ligase RNF146 | RNF146 | 42 | Annotation score: | ||
A0A0G2JLL7 | A0A0G2JLL7_HUMAN | E3 ubiquitin-protein ligase RNF146 | RNF146 | 9 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 | K → R in BAB55359 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 74 | C → R in BAB55108 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 100 | G → E in CAG38545 (Ref. 4) Curated | 1 | |
Sequence conflicti | 166 | I → V in CAG38545 (Ref. 4) Curated | 1 | |
Sequence conflicti | 229 | L → S in BAB55108 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 329 | S → L in BAB55108 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_065249 | 25 | C → R. Corresponds to variant dbSNP:rs10081141Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012968 | 1 | Missing in isoform 2. 5 Publications | 1 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000309649; ENSP00000309365; ENSG00000118518 [Q9NTX7-2] ENST00000368314; ENSP00000357297; ENSG00000118518 [Q9NTX7-1] ENST00000608991; ENSP00000477168; ENSG00000118518 [Q9NTX7-2] ENST00000610153; ENSP00000476814; ENSG00000118518 [Q9NTX7-1] ENST00000616343; ENSP00000479890; ENSG00000118518 [Q9NTX7-1] |
GeneIDi | 81847 |
KEGGi | hsa:81847 |
UCSCi | uc003qav.4, human [Q9NTX7-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2D8T | NMR | - | A | 27-84 | [»] | |
3V3L | X-ray | 1.65 | A/B | 100-184 | [»] | |
6CF6 | X-ray | 1.93 | C/D | 191-203 | [»] | |
SMRi | Q9NTX7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123598, 59 interactors |
DIPi | DIP-52730N |
IntActi | Q9NTX7, 46 interactors |
STRINGi | 9606.ENSP00000357297 |
PTM databases
iPTMneti | Q9NTX7 |
PhosphoSitePlusi | Q9NTX7 |
Polymorphism and mutation databases
BioMutai | RNF146 |
DMDMi | 60390653 |
Proteomic databases
jPOSTi | Q9NTX7 |
MassIVEi | Q9NTX7 |
MaxQBi | Q9NTX7 |
PaxDbi | Q9NTX7 |
PeptideAtlasi | Q9NTX7 |
PRIDEi | Q9NTX7 |
ProteomicsDBi | 82642 [Q9NTX7-1] 82643 [Q9NTX7-2] |
Protocols and materials databases
Antibodypediai | 19579, 65 antibodies |
DNASUi | 81847 |
Genome annotation databases
Ensembli | ENST00000309649; ENSP00000309365; ENSG00000118518 [Q9NTX7-2] ENST00000368314; ENSP00000357297; ENSG00000118518 [Q9NTX7-1] ENST00000608991; ENSP00000477168; ENSG00000118518 [Q9NTX7-2] ENST00000610153; ENSP00000476814; ENSG00000118518 [Q9NTX7-1] ENST00000616343; ENSP00000479890; ENSG00000118518 [Q9NTX7-1] |
GeneIDi | 81847 |
KEGGi | hsa:81847 |
UCSCi | uc003qav.4, human [Q9NTX7-1] |
Organism-specific databases
CTDi | 81847 |
DisGeNETi | 81847 |
EuPathDBi | HostDB:ENSG00000118518.15 |
GeneCardsi | RNF146 |
HGNCi | HGNC:21336, RNF146 |
HPAi | ENSG00000118518, Low tissue specificity |
MIMi | 612137, gene |
neXtProti | NX_Q9NTX7 |
OpenTargetsi | ENSG00000118518 |
PharmGKBi | PA134910489 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0824, Eukaryota |
GeneTreei | ENSGT00390000000358 |
HOGENOMi | CLU_067425_0_0_1 |
InParanoidi | Q9NTX7 |
OMAi | AHLQING |
OrthoDBi | 1469576at2759 |
PhylomeDBi | Q9NTX7 |
TreeFami | TF318925 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q9NTX7 |
Reactomei | R-HSA-201681, TCF dependent signaling in response to WNT R-HSA-4641257, Degradation of AXIN R-HSA-5689880, Ub-specific processing proteases R-HSA-8948751, Regulation of PTEN stability and activity |
SIGNORi | Q9NTX7 |
Miscellaneous databases
BioGRID-ORCSi | 81847, 15 hits in 850 CRISPR screens |
ChiTaRSi | RNF146, human |
EvolutionaryTracei | Q9NTX7 |
GeneWikii | RNF146 |
GenomeRNAii | 81847 |
Pharosi | Q9NTX7, Tbio |
PROi | PR:Q9NTX7 |
RNActi | Q9NTX7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000118518, Expressed in cerebellar vermis and 230 other tissues |
ExpressionAtlasi | Q9NTX7, baseline and differential |
Genevisiblei | Q9NTX7, HS |
Family and domain databases
CDDi | cd16546, RING-HC_RNF146, 1 hit |
Gene3Di | 3.30.40.10, 1 hit 3.30.720.50, 1 hit |
InterProi | View protein in InterPro IPR044110, RING-HC_RNF146 IPR033509, RNF146 IPR004170, WWE-dom IPR018123, WWE-dom_subgr IPR037197, WWE_dom_sf IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD IPR017907, Znf_RING_CS |
PANTHERi | PTHR13417:SF2, PTHR13417:SF2, 1 hit |
Pfami | View protein in Pfam PF02825, WWE, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit SM00678, WWE, 1 hit |
SUPFAMi | SSF117839, SSF117839, 1 hit |
PROSITEi | View protein in PROSITE PS50918, WWE, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RN146_HUMAN | |
Accessioni | Q9NTX7Primary (citable) accession number: Q9NTX7 Secondary accession number(s): E1P572 Q9NTX6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 1, 2005 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations