UniProtKB - Q9NSI5 (IGSF5_HUMAN)
Protein
Immunoglobulin superfamily member 5
Gene
IGSF5
Organism
Homo sapiens (Human)
Status
Functioni
Provides, together with MAGI1, an adhesion machinery at tight junctions, which may regulate the permeability of kidney glomerulus and small intestinal epithelial cells. Mediates calcium-independent homophilic cell adhesion. In testis, it may function as a cell adhesion molecule rather than a tight-junction protein. It may participate in the adhesion between spermatogonia-spermatogonia, spermatogonia-Sertoli cells, and Sertoli cells-Sertoli cells (By similarity).By similarity
Miscellaneous
A protein of the expected size has been detected by antibody binding and Western blot in at least one of the analyzed tissues or cells.1 Publication
GO - Biological processi
- cell-cell adhesion Source: GO_Central
Enzyme and pathway databases
PathwayCommonsi | Q9NSI5 |
Names & Taxonomyi
Protein namesi | Recommended name: Immunoglobulin superfamily member 5Short name: IgSF5 Alternative name(s): Junctional adhesion molecule 4 Short name: JAM-4 |
Gene namesi | Name:IGSF5 Synonyms:JAM4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000183067.5 |
HGNCi | HGNC:5952, IGSF5 |
MIMi | 610638, gene |
neXtProti | NX_Q9NSI5 |
Subcellular locationi
Plasma membrane
- Apical cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Other locations
- tight junction By similarity
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
Other locations
- bicellular tight junction Source: GO_Central
- cell surface Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 266 | ExtracellularSequence analysisAdd BLAST | 266 | |
Transmembranei | 267 – 285 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 286 – 407 | CytoplasmicSequence analysisAdd BLAST | 122 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Organism-specific databases
DisGeNETi | 150084 |
OpenTargetsi | ENSG00000183067 |
PharmGKBi | PA29765 |
Miscellaneous databases
Pharosi | Q9NSI5, Tbio |
Polymorphism and mutation databases
BioMutai | IGSF5 |
DMDMi | 166918602 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000316295 | 1 – 407 | Immunoglobulin superfamily member 5Add BLAST | 407 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 59 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 60 ↔ 123 | PROSITE-ProRule annotation | ||
Glycosylationi | 103 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 163 ↔ 215 | PROSITE-ProRule annotation | ||
Glycosylationi | 210 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 231 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9NSI5 |
PRIDEi | Q9NSI5 |
ProteomicsDBi | 82554 |
PTM databases
GlyConnecti | 1393, 1 N-Linked glycan (1 site) |
GlyGeni | Q9NSI5, 4 sites |
iPTMneti | Q9NSI5 |
PhosphoSitePlusi | Q9NSI5 |
Expressioni
Gene expression databases
Bgeei | ENSG00000183067, Expressed in placenta and 106 other tissues |
Genevisiblei | Q9NSI5, HS |
Organism-specific databases
HPAi | ENSG00000183067, Tissue enhanced (heart) |
Interactioni
Subunit structurei
Interacts with MAGI1 at tight junctions, forms a tripartite complex with NPHS1.
Interacts with LNX1 isoform 2 via its PDZ 2 domain, it may also interact with other isoforms containing this domain.
By similarityProtein-protein interaction databases
BioGRIDi | 127259, 3 interactors |
STRINGi | 9606.ENSP00000369962 |
Miscellaneous databases
RNActi | Q9NSI5, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 139 | Ig-like V-type 1Add BLAST | 101 | |
Domaini | 142 – 231 | Ig-like V-type 2Add BLAST | 90 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 281 – 307 | Cys-richAdd BLAST | 27 |
Sequence similaritiesi
Belongs to the immunoglobulin superfamily.Curated
Keywords - Domaini
Immunoglobulin domain, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S015, Eukaryota |
GeneTreei | ENSGT00940000163947 |
HOGENOMi | CLU_063888_1_0_1 |
InParanoidi | Q9NSI5 |
OMAi | HSSYYFV |
PhylomeDBi | Q9NSI5 |
TreeFami | TF335729 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR043364, IgSF5 |
PANTHERi | PTHR44991, PTHR44991, 1 hit |
Pfami | View protein in Pfam PF07679, I-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
i Sequence
Sequence statusi: Complete.
Q9NSI5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGQKERSTAD TLPDLEEWKS AAGLRWWQTA VVDGSGSGNE VIEGPQNARV
60 70 80 90 100
LKGSQARFNC TVSQGWKLIM WALSDMVVLS VRPMEPIITN DRFTSQRYDQ
110 120 130 140 150
GGNFTSEMII HNVEPSDSGN IRCSLQNSRL HGSAYLTVQV MGELFIPSVN
160 170 180 190 200
LVVAENEPCE VTCLPSHWTR LPDISWELGL LVSHSSYYFV PEPSDLQSAV
210 220 230 240 250
SILALTPQSN GTLTCVATWK SLKARKSATV NLTVIRCPQD TGGGINIPGV
260 270 280 290 300
LSSLPSLGFS LPTWGKVGLG LAGTMLLTPT CTLTIRCCCC RRRCCGCNCC
310 320 330 340 350
CRCCFCCRRK RGFRIQFQKK SEKEKTNKET ETESGNENSG YNSDEQKTTD
360 370 380 390 400
TASLPPKSCE SSDPEQRNSS CGPPHQRADQ RPPRPASHPQ ASFNLASPEK
VSNTTVV
Sequence cautioni
The sequence CAB90447 differs from that shown. Reason: Erroneous gene model prediction.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_038381 | 49 | R → T1 PublicationCorresponds to variant dbSNP:rs2205204Ensembl. | 1 | |
Natural variantiVAR_038382 | 156 | N → K. Corresponds to variant dbSNP:rs11908882Ensembl. | 1 | |
Natural variantiVAR_038383 | 170 | R → W. Corresponds to variant dbSNP:rs8129968Ensembl. | 1 | |
Natural variantiVAR_038384 | 350 | D → E1 PublicationCorresponds to variant dbSNP:rs2837225Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK092516 mRNA No translation available. AL163280 Genomic DNA Translation: CAB90447.1 Sequence problems. |
CCDSi | CCDS33562.1 |
RefSeqi | NP_001073913.1, NM_001080444.1 |
Genome annotation databases
Ensembli | ENST00000380588; ENSP00000369962; ENSG00000183067 |
GeneIDi | 150084 |
KEGGi | hsa:150084 |
UCSCi | uc002yyo.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK092516 mRNA No translation available. AL163280 Genomic DNA Translation: CAB90447.1 Sequence problems. |
CCDSi | CCDS33562.1 |
RefSeqi | NP_001073913.1, NM_001080444.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 127259, 3 interactors |
STRINGi | 9606.ENSP00000369962 |
PTM databases
GlyConnecti | 1393, 1 N-Linked glycan (1 site) |
GlyGeni | Q9NSI5, 4 sites |
iPTMneti | Q9NSI5 |
PhosphoSitePlusi | Q9NSI5 |
Polymorphism and mutation databases
BioMutai | IGSF5 |
DMDMi | 166918602 |
Proteomic databases
PaxDbi | Q9NSI5 |
PRIDEi | Q9NSI5 |
ProteomicsDBi | 82554 |
Protocols and materials databases
Antibodypediai | 54406, 110 antibodies |
DNASUi | 150084 |
Genome annotation databases
Ensembli | ENST00000380588; ENSP00000369962; ENSG00000183067 |
GeneIDi | 150084 |
KEGGi | hsa:150084 |
UCSCi | uc002yyo.3, human |
Organism-specific databases
CTDi | 150084 |
DisGeNETi | 150084 |
EuPathDBi | HostDB:ENSG00000183067.5 |
GeneCardsi | IGSF5 |
HGNCi | HGNC:5952, IGSF5 |
HPAi | ENSG00000183067, Tissue enhanced (heart) |
MIMi | 610638, gene |
neXtProti | NX_Q9NSI5 |
OpenTargetsi | ENSG00000183067 |
PharmGKBi | PA29765 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S015, Eukaryota |
GeneTreei | ENSGT00940000163947 |
HOGENOMi | CLU_063888_1_0_1 |
InParanoidi | Q9NSI5 |
OMAi | HSSYYFV |
PhylomeDBi | Q9NSI5 |
TreeFami | TF335729 |
Enzyme and pathway databases
PathwayCommonsi | Q9NSI5 |
Miscellaneous databases
BioGRID-ORCSi | 150084, 34 hits in 843 CRISPR screens |
ChiTaRSi | IGSF5, human |
GenomeRNAii | 150084 |
Pharosi | Q9NSI5, Tbio |
PROi | PR:Q9NSI5 |
RNActi | Q9NSI5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000183067, Expressed in placenta and 106 other tissues |
Genevisiblei | Q9NSI5, HS |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR043364, IgSF5 |
PANTHERi | PTHR44991, PTHR44991, 1 hit |
Pfami | View protein in Pfam PF07679, I-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IGSF5_HUMAN | |
Accessioni | Q9NSI5Primary (citable) accession number: Q9NSI5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 5, 2008 |
Last sequence update: | February 5, 2008 | |
Last modified: | December 2, 2020 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 21
Human chromosome 21: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations