UniProtKB - Q9NS23 (RASF1_HUMAN)
Ras association domain-containing protein 1
RASSF1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 51 – 105 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 55 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein heterodimerization activity Source: CAFA
- protein N-terminus binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- cell cycle arrest Source: UniProtKB
- cellular response to DNA damage stimulus Source: UniProtKB
- negative regulation of cell cycle arrest Source: UniProtKB
- positive regulation of protein ubiquitination Source: UniProtKB
- protein stabilization Source: UniProtKB
- Ras protein signal transduction Source: UniProtKB
- regulation of microtubule cytoskeleton organization Source: InterPro
Keywordsi
Biological process | Cell cycle |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
SignaLinki | Q9NS23 |
SIGNORi | Q9NS23 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras association domain-containing protein 1 |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9882 RASSF1 |
MIMi | 605082 gene |
neXtProti | NX_Q9NS23 |
Subcellular locationi
Cytoskeleton
Nucleus
Note: Localizes to cytoplasmic microtubules during interphase, to bipolar centrosomes associated with microtubules during prophase, to spindle fibers and spindle poles at metaphase and anaphase, to the midzone during early telophase, and to the midbody in late telophase in cells. Colocalizes with MDM2 in the nucleus.
Cytoskeleton
- microtubule Source: UniProtKB-KW
- microtubule cytoskeleton Source: UniProtKB
- microtubule organizing center Source: UniProtKB-SubCell
- spindle pole Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPathology & Biotechi
Keywords - Diseasei
Tumor suppressorOrganism-specific databases
DisGeNETi | 11186 |
OpenTargetsi | ENSG00000068028 |
PharmGKBi | PA34245 |
Miscellaneous databases
Pharosi | Q9NS23 |
Polymorphism and mutation databases
BioMutai | RASSF1 |
DMDMi | 50401686 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000068891 | 2 – 344 | Ras association domain-containing protein 1Add BLAST | 343 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 2 | PhosphoserineCombined sources | 1 | |
Modified residuei | 36 | Omega-N-methylarginineCombined sources | 1 |
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
jPOSTi | Q9NS23 |
MassIVEi | Q9NS23 |
MaxQBi | Q9NS23 |
PaxDbi | Q9NS23 |
PeptideAtlasi | Q9NS23 |
PRIDEi | Q9NS23 |
ProteomicsDBi | 82465 [Q9NS23-1] 82466 [Q9NS23-2] 82467 [Q9NS23-3] 82468 [Q9NS23-4] 82469 [Q9NS23-5] 82470 [Q9NS23-6] 82471 [Q9NS23-7] 82472 [Q9NS23-9] |
PTM databases
iPTMneti | Q9NS23 |
PhosphoSitePlusi | Q9NS23 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000068028 Expressed in 170 organ(s), highest expression level in small intestine Peyer's patch |
Genevisiblei | Q9NS23 HS |
Organism-specific databases
HPAi | HPA040735 |
Interactioni
Subunit structurei
Interacts with MAP1S (PubMed:15205320, PubMed:15753381).
Interacts with XPA (PubMed:10888881). Binds to the N-terminal of CDC20 during prometaphase (PubMed:14743218). Binds to STK3/MST2 and STK4/MST1 (PubMed:15109305, PubMed:16510573, PubMed:21199877). Isoform A interacts with MOAP1 (PubMed:15949439). Isoform A interacts with E4F1 (PubMed:14729613). Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization (PubMed:15949439). Can self-associate (PubMed:11857081). Isoform A interacts with RSSF5 and probably associates with HRAS via a RSSF1 isoform A-RSSF5 heterodimer (PubMed:11857081).
Part of a complex with MDM2, DAXX, RASSF1 and USP7 (PubMed:18566590). Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53 (PubMed:18566590). Isoform C or isoform H interacts (via N-terminus) with DAXX (PubMed:18566590). Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53 (PubMed:18566590).
Interacts with ECM2 (By similarity).
By similarity11 PublicationsBinary interactionsi
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein heterodimerization activity Source: CAFA
- protein N-terminus binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 116356, 91 interactors |
DIPi | DIP-31270N |
IntActi | Q9NS23, 45 interactors |
MINTi | Q9NS23 |
STRINGi | 9606.ENSP00000349547 |
Structurei
Secondary structure
3D structure databases
SMRi | Q9NS23 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NS23 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 198 – 292 | Ras-associatingPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 294 – 341 | SARAHPROSITE-ProRule annotationAdd BLAST | 48 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 119 | Mediates interaction with E4F11 PublicationAdd BLAST | 118 | |
Regioni | 315 – 318 | MOAP1-binding | 4 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 51 – 105 | Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST | 55 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG410IPAE Eukaryota ENOG4111HS2 LUCA |
GeneTreei | ENSGT00940000155664 |
HOGENOMi | HOG000013025 |
InParanoidi | Q9NS23 |
KOi | K09850 |
OMAi | SFEMTWG |
OrthoDBi | 1120975at2759 |
PhylomeDBi | Q9NS23 |
TreeFami | TF319243 |
Family and domain databases
CDDi | cd00029 C1, 1 hit |
DisProti | DP01533 [Q9NS23-4] |
InterProi | View protein in InterPro IPR033614 C-RASSF IPR002219 PE/DAG-bd IPR000159 RA_dom IPR033600 RASSF1 IPR011524 SARAH_dom IPR029071 Ubiquitin-like_domsf |
PANTHERi | PTHR22738 PTHR22738, 1 hit PTHR22738:SF12 PTHR22738:SF12, 1 hit |
Pfami | View protein in Pfam PF16517 Nore1-SARAH, 1 hit PF00788 RA, 1 hit |
SMARTi | View protein in SMART SM00109 C1, 1 hit SM00314 RA, 1 hit |
SUPFAMi | SSF54236 SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50200 RA, 1 hit PS50951 SARAH, 1 hit PS50081 ZF_DAG_PE_2, 1 hit |
s (8)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 8 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGEPELIEL RELAPAGRAG KGRTRLERAN ALRIARGTAC NPTRQLVPGR
60 70 80 90 100
GHRFQPAGPA THTWCDLCGD FIWGVVRKGL QCARLSADCK FTCHYRCRAL
110 120 130 140 150
VCLDCCGPRD LGWEPAVERD TNVDEPVEWE TPDLSQAEIE QKIKEYNAQI
160 170 180 190 200
NSNLFMSLNK DGSYTGFIKV QLKLVRPVSV PSSKKPPSLQ DARRGPGRGT
210 220 230 240 250
SVRRRTSFYL PKDAVKHLHV LSRTRAREVI EALLRKFLVV DDPRKFALFE
260 270 280 290 300
RAERHGQVYL RKLLDDEQPL RLRLLAGPSD KALSFVLKEN DSGEVNWDAF
310 320 330 340
SMPELHNFLR ILQREEEEHL RQILQKYSYC RQKIQEALHA CPLG
The sequence of this isoform differs from the canonical sequence as follows:
84-149: RLSADCKFTC...EQKIKEYNAQ → QQGRFLHRLH...PACAVTHKGT
150-344: Missing.
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_019542 | 21 | K → Q2 PublicationsCorresponds to variant dbSNP:rs4688725Ensembl. | 1 | |
Natural variantiVAR_019543 | 53 | R → C1 PublicationCorresponds to variant dbSNP:rs201618726Ensembl. | 1 | |
Natural variantiVAR_019544 | 133 | D → E1 PublicationCorresponds to variant dbSNP:rs76335415Ensembl. | 1 | |
Natural variantiVAR_019545 | 135 | S → F Prevents G1 cell cycle arrest; reduced protein phosphorylation. 1 PublicationCorresponds to variant dbSNP:rs934370004Ensembl. | 1 | |
Natural variantiVAR_019546 | 137 | A → S Prevents G1 cell cycle arrest; reduced protein phosphorylation. 2 PublicationsCorresponds to variant dbSNP:rs2073498Ensembl. | 1 | |
Natural variantiVAR_059794 | 319 | H → R. Corresponds to variant dbSNP:rs12488879Ensembl. | 1 | |
Natural variantiVAR_019547 | 329 | Y → C1 PublicationCorresponds to variant dbSNP:rs782655006Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_050770 | 1 – 155 | Missing in isoform B. 2 PublicationsAdd BLAST | 155 | |
Alternative sequenceiVSP_050773 | 1 – 74 | Missing in isoform C and isoform H. 3 PublicationsAdd BLAST | 74 | |
Alternative sequenceiVSP_050774 | 75 – 123 | VVRKG…RDTNV → MGEAEAPSFEMTWSSTTSSG YCSQEDSDSELEQYFTARTS LARRPRRDQ in isoform C and isoform H. 3 PublicationsAdd BLAST | 49 | |
Alternative sequenceiVSP_050775 | 84 – 149 | RLSAD…EYNAQ → QQGRFLHRLHQGSAEAGAPC LCALQQEATLLAGCPAGPRT GHKCQAPHFLLPAQGCCQAP ACAVTHKGT in isoform G. 1 PublicationAdd BLAST | 66 | |
Alternative sequenceiVSP_050771 | 84 – 88 | RLSAD → H in isoform A and isoform E. 3 Publications | 5 | |
Alternative sequenceiVSP_050776 | 85 – 91 | LSADCKF → RACGVGD in isoform F. 1 Publication | 7 | |
Alternative sequenceiVSP_050777 | 93 – 344 | Missing in isoform F. 1 PublicationAdd BLAST | 252 | |
Alternative sequenceiVSP_050772 | 123 | V → VPILQ in isoform E. 1 Publication | 1 | |
Alternative sequenceiVSP_050778 | 150 – 344 | Missing in isoform G and isoform H. 1 PublicationAdd BLAST | 195 |
Sequence databases
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KZU | NMR | - | B | - | [»] | |
SMRi | Q9NS23 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGridi | 116356, 91 interactors |
DIPi | DIP-31270N |
IntActi | Q9NS23, 45 interactors |
MINTi | Q9NS23 |
STRINGi | 9606.ENSP00000349547 |
PTM databases
iPTMneti | Q9NS23 |
PhosphoSitePlusi | Q9NS23 |
Polymorphism and mutation databases
BioMutai | RASSF1 |
DMDMi | 50401686 |
Proteomic databases
jPOSTi | Q9NS23 |
MassIVEi | Q9NS23 |
MaxQBi | Q9NS23 |
PaxDbi | Q9NS23 |
PeptideAtlasi | Q9NS23 |
PRIDEi | Q9NS23 |
ProteomicsDBi | 82465 [Q9NS23-1] 82466 [Q9NS23-2] 82467 [Q9NS23-3] 82468 [Q9NS23-4] 82469 [Q9NS23-5] 82470 [Q9NS23-6] 82471 [Q9NS23-7] 82472 [Q9NS23-9] |
Protocols and materials databases
DNASUi | 11186 |
Genome annotation databases
Organism-specific databases
CTDi | 11186 |
DisGeNETi | 11186 |
GeneCardsi | RASSF1 |
HGNCi | HGNC:9882 RASSF1 |
HPAi | HPA040735 |
MIMi | 605082 gene |
neXtProti | NX_Q9NS23 |
OpenTargetsi | ENSG00000068028 |
PharmGKBi | PA34245 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG410IPAE Eukaryota ENOG4111HS2 LUCA |
GeneTreei | ENSGT00940000155664 |
HOGENOMi | HOG000013025 |
InParanoidi | Q9NS23 |
KOi | K09850 |
OMAi | SFEMTWG |
OrthoDBi | 1120975at2759 |
PhylomeDBi | Q9NS23 |
TreeFami | TF319243 |
Enzyme and pathway databases
SignaLinki | Q9NS23 |
SIGNORi | Q9NS23 |
Miscellaneous databases
ChiTaRSi | RASSF1 human |
EvolutionaryTracei | Q9NS23 |
GeneWikii | RASSF1 |
GenomeRNAii | 11186 |
Pharosi | Q9NS23 |
PROi | PR:Q9NS23 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000068028 Expressed in 170 organ(s), highest expression level in small intestine Peyer's patch |
Genevisiblei | Q9NS23 HS |
Family and domain databases
CDDi | cd00029 C1, 1 hit |
DisProti | DP01533 [Q9NS23-4] |
InterProi | View protein in InterPro IPR033614 C-RASSF IPR002219 PE/DAG-bd IPR000159 RA_dom IPR033600 RASSF1 IPR011524 SARAH_dom IPR029071 Ubiquitin-like_domsf |
PANTHERi | PTHR22738 PTHR22738, 1 hit PTHR22738:SF12 PTHR22738:SF12, 1 hit |
Pfami | View protein in Pfam PF16517 Nore1-SARAH, 1 hit PF00788 RA, 1 hit |
SMARTi | View protein in SMART SM00109 C1, 1 hit SM00314 RA, 1 hit |
SUPFAMi | SSF54236 SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50200 RA, 1 hit PS50951 SARAH, 1 hit PS50081 ZF_DAG_PE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RASF1_HUMAN | |
Accessioni | Q9NS23Primary (citable) accession number: Q9NS23 Secondary accession number(s): B7ZLL1 Q9UND5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | November 13, 2019 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references