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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase gamma

Gene

AGPAT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Acts on LPA containing saturated or unsaturated fatty acids C16:0-C20:4 at the sn-1 position using C18:1, C20:4 or C18:2-CoA as the acyl donor. Also acts on lysophosphatidylcholine, lysophosphatidylinositol and lysophosphatidylserine using C18:1 or C20:4-CoA (PubMed:21173190). Has a preference for arachidonoyl-CoA as a donor. Has also a modest lysophosphatidylinositol acyltransferase (LPIAT) activity, converts lysophosphatidylinositol (LPI) into phosphatidylinositol (By similarity).By similarity1 Publication

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Kineticsi

  1. KM=4.78 µM for LPA sn-1 C18:11 Publication
  2. KM=21.53 µM for C18:1-CoA1 Publication
  1. Vmax=6.35 nmol/min/mg enzyme toward LPA sn-1 C18:11 Publication
  2. Vmax=0.74 nmol/min/mg enzyme toward C18:1-CoA1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), Glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 1, mitochondrial (DKFZp451B1115), Glycerol-3-phosphate acyltransferase 1, mitochondrial, Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4), Lysocardiolipin acyltransferase 1 (LCLAT1)
  3. Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase (cdsA), Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS08470-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
SIGNORiQ9NRZ7
UniPathwayi
UPA00557;UER00613

Chemistry databases

SwissLipidsiSLP:000000823

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (EC:2.3.1.51)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 3
Short name:
1-AGP acyltransferase 3
Short name:
1-AGPAT 3
Lysophosphatidic acid acyltransferase gamma
Short name:
LPAAT-gamma
Gene namesi
Name:AGPAT3
Synonyms:LPAAT3
ORF Names:UNQ759/PRO1490
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000160216.18
HGNCiHGNC:326 AGPAT3
MIMi614794 gene
neXtProtiNX_Q9NRZ7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 124CytoplasmicSequence analysisAdd BLAST124
Transmembranei125 – 145HelicalSequence analysisAdd BLAST21
Topological domaini146 – 316LumenalSequence analysisAdd BLAST171
Transmembranei317 – 339HelicalSequence analysisAdd BLAST23
Topological domaini340 – 376CytoplasmicSequence analysisAdd BLAST37

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi56894
OpenTargetsiENSG00000160216
PharmGKBiPA24623

Polymorphism and mutation databases

BioMutaiAGPAT3
DMDMi12643817

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002081941 – 3761-acyl-sn-glycerol-3-phosphate acyltransferase gammaAdd BLAST376

Proteomic databases

EPDiQ9NRZ7
MaxQBiQ9NRZ7
PaxDbiQ9NRZ7
PeptideAtlasiQ9NRZ7
PRIDEiQ9NRZ7
ProteomicsDBi82447
82448 [Q9NRZ7-2]
82449 [Q9NRZ7-3]

PTM databases

iPTMnetiQ9NRZ7
PhosphoSitePlusiQ9NRZ7
SwissPalmiQ9NRZ7

Expressioni

Tissue specificityi

Widely expressed with highest levels in testis, pancreas and kidney, followed by spleen, lung, adipose tissue and liver.1 Publication

Gene expression databases

BgeeiENSG00000160216 Expressed in 204 organ(s), highest expression level in primary visual cortex
CleanExiHS_AGPAT3
ExpressionAtlasiQ9NRZ7 baseline and differential
GenevisibleiQ9NRZ7 HS

Interactioni

Protein-protein interaction databases

BioGridi121224, 36 interactors
IntActiQ9NRZ7, 18 interactors
MINTiQ9NRZ7
STRINGi9606.ENSP00000291572

Structurei

3D structure databases

ProteinModelPortaliQ9NRZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 101HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00890000139404
HOVERGENiHBG008205
InParanoidiQ9NRZ7
KOiK13523
OMAiFLFWATV
OrthoDBiEOG091G0C4Z
PhylomeDBiQ9NRZ7
TreeFamiTF314065

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NRZ7-1) [UniParc]FASTAAdd to basket
Also known as: Gamma-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGLLAFLKTQ FVLHLLVGFV FVVSGLVINF VQLCTLALWP VSKQLYRRLN
60 70 80 90 100
CRLAYSLWSQ LVMLLEWWSC TECTLFTDQA TVERFGKEHA VIILNHNFEI
110 120 130 140 150
DFLCGWTMCE RFGVLGSSKV LAKKELLYVP LIGWTWYFLE IVFCKRKWEE
160 170 180 190 200
DRDTVVEGLR RLSDYPEYMW FLLYCEGTRF TETKHRVSME VAAAKGLPVL
210 220 230 240 250
KYHLLPRTKG FTTAVKCLRG TVAAVYDVTL NFRGNKNPSL LGILYGKKYE
260 270 280 290 300
ADMCVRRFPL EDIPLDEKEA AQWLHKLYQE KDALQEIYNQ KGMFPGEQFK
310 320 330 340 350
PARRPWTLLN FLSWATILLS PLFSFVLGVF ASGSPLLILT FLGFVGAASF
360 370
GVRRLIGVTE IEKGSSYGNQ EFKKKE
Length:376
Mass (Da):43,381
Last modified:October 1, 2000 - v1
Checksum:iC12CDBB7CC363852
GO
Isoform 2 (identifier: Q9NRZ7-2) [UniParc]FASTAAdd to basket
Also known as: Gamma-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Show »
Length:314
Mass (Da):36,271
Checksum:iFFB9EA98F4F5C204
GO
Isoform 3 (identifier: Q9NRZ7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: MGLLAFLKTQ...CRLAYSLWSQ → MQSGGSLPFC...SANALPLSAE

Note: No experimental confirmation available.
Show »
Length:396
Mass (Da):45,514
Checksum:i9729B3AE9FB5E518
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JL26C9JL26_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT3
255Annotation score:
C9J184C9J184_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT3
128Annotation score:
C9JQX8C9JQX8_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT3
79Annotation score:
C9JK35C9JK35_HUMAN
1-acyl-sn-glycerol-3-phosphate acyl...
AGPAT3
38Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74T → P in CAD38635 (PubMed:14702039).Curated1
Sequence conflicti358 – 376VTEIE…FKKKE → ESLEPGRWRLQ in AAQ89067 (PubMed:12975309).CuratedAdd BLAST19

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0050721 – 62Missing in isoform 2. 1 PublicationAdd BLAST62
Alternative sequenceiVSP_0131441 – 60MGLLA…SLWSQ → MQSGGSLPFCCYLPSVSSQL LLRESYCNFIKRTQCKSSKL MFSRDFLSGQKYCRCLLWAL PDHPRRRGPTSANALPLSAE in isoform 3. 1 PublicationAdd BLAST60

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156774 mRNA Translation: AAF80336.1
AF156775 mRNA Translation: AAF80337.1
AB040138 mRNA Translation: BAB18943.1
AY358704 mRNA Translation: AAQ89067.1
AP001054 Genomic DNA No translation available.
CH471079 Genomic DNA Translation: EAX09461.1
CH471079 Genomic DNA Translation: EAX09463.1
CH471079 Genomic DNA Translation: EAX09464.1
CH471079 Genomic DNA Translation: EAX09465.1
BC011971 mRNA Translation: AAH11971.1
BC040603 mRNA Translation: AAH40603.1
BC063552 mRNA Translation: AAH63552.1
AK125804 mRNA Translation: BAC86299.1
AL832919 mRNA Translation: CAD38635.2
CCDSiCCDS13703.1 [Q9NRZ7-1]
RefSeqiNP_001032642.1, NM_001037553.1 [Q9NRZ7-1]
NP_064517.1, NM_020132.4 [Q9NRZ7-1]
XP_005261217.1, XM_005261160.4 [Q9NRZ7-1]
XP_006724092.1, XM_006724029.3 [Q9NRZ7-1]
XP_006724093.1, XM_006724030.3 [Q9NRZ7-1]
XP_006724094.1, XM_006724031.3 [Q9NRZ7-2]
XP_011527967.1, XM_011529665.2 [Q9NRZ7-1]
XP_016883898.1, XM_017028409.1 [Q9NRZ7-1]
XP_016883899.1, XM_017028410.1 [Q9NRZ7-1]
XP_016883900.1, XM_017028411.1 [Q9NRZ7-2]
XP_016883901.1, XM_017028412.1 [Q9NRZ7-2]
XP_016883902.1, XM_017028413.1 [Q9NRZ7-2]
UniGeneiHs.248785

Genome annotation databases

EnsembliENST00000291572; ENSP00000291572; ENSG00000160216 [Q9NRZ7-1]
ENST00000327505; ENSP00000332989; ENSG00000160216 [Q9NRZ7-1]
ENST00000398058; ENSP00000381135; ENSG00000160216 [Q9NRZ7-1]
ENST00000398061; ENSP00000381138; ENSG00000160216 [Q9NRZ7-1]
ENST00000398063; ENSP00000381140; ENSG00000160216 [Q9NRZ7-1]
ENST00000546158; ENSP00000443510; ENSG00000160216 [Q9NRZ7-1]
GeneIDi56894
KEGGihsa:56894
UCSCiuc002zdv.4 human [Q9NRZ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF156774 mRNA Translation: AAF80336.1
AF156775 mRNA Translation: AAF80337.1
AB040138 mRNA Translation: BAB18943.1
AY358704 mRNA Translation: AAQ89067.1
AP001054 Genomic DNA No translation available.
CH471079 Genomic DNA Translation: EAX09461.1
CH471079 Genomic DNA Translation: EAX09463.1
CH471079 Genomic DNA Translation: EAX09464.1
CH471079 Genomic DNA Translation: EAX09465.1
BC011971 mRNA Translation: AAH11971.1
BC040603 mRNA Translation: AAH40603.1
BC063552 mRNA Translation: AAH63552.1
AK125804 mRNA Translation: BAC86299.1
AL832919 mRNA Translation: CAD38635.2
CCDSiCCDS13703.1 [Q9NRZ7-1]
RefSeqiNP_001032642.1, NM_001037553.1 [Q9NRZ7-1]
NP_064517.1, NM_020132.4 [Q9NRZ7-1]
XP_005261217.1, XM_005261160.4 [Q9NRZ7-1]
XP_006724092.1, XM_006724029.3 [Q9NRZ7-1]
XP_006724093.1, XM_006724030.3 [Q9NRZ7-1]
XP_006724094.1, XM_006724031.3 [Q9NRZ7-2]
XP_011527967.1, XM_011529665.2 [Q9NRZ7-1]
XP_016883898.1, XM_017028409.1 [Q9NRZ7-1]
XP_016883899.1, XM_017028410.1 [Q9NRZ7-1]
XP_016883900.1, XM_017028411.1 [Q9NRZ7-2]
XP_016883901.1, XM_017028412.1 [Q9NRZ7-2]
XP_016883902.1, XM_017028413.1 [Q9NRZ7-2]
UniGeneiHs.248785

3D structure databases

ProteinModelPortaliQ9NRZ7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121224, 36 interactors
IntActiQ9NRZ7, 18 interactors
MINTiQ9NRZ7
STRINGi9606.ENSP00000291572

Chemistry databases

SwissLipidsiSLP:000000823

PTM databases

iPTMnetiQ9NRZ7
PhosphoSitePlusiQ9NRZ7
SwissPalmiQ9NRZ7

Polymorphism and mutation databases

BioMutaiAGPAT3
DMDMi12643817

Proteomic databases

EPDiQ9NRZ7
MaxQBiQ9NRZ7
PaxDbiQ9NRZ7
PeptideAtlasiQ9NRZ7
PRIDEiQ9NRZ7
ProteomicsDBi82447
82448 [Q9NRZ7-2]
82449 [Q9NRZ7-3]

Protocols and materials databases

DNASUi56894
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291572; ENSP00000291572; ENSG00000160216 [Q9NRZ7-1]
ENST00000327505; ENSP00000332989; ENSG00000160216 [Q9NRZ7-1]
ENST00000398058; ENSP00000381135; ENSG00000160216 [Q9NRZ7-1]
ENST00000398061; ENSP00000381138; ENSG00000160216 [Q9NRZ7-1]
ENST00000398063; ENSP00000381140; ENSG00000160216 [Q9NRZ7-1]
ENST00000546158; ENSP00000443510; ENSG00000160216 [Q9NRZ7-1]
GeneIDi56894
KEGGihsa:56894
UCSCiuc002zdv.4 human [Q9NRZ7-1]

Organism-specific databases

CTDi56894
DisGeNETi56894
EuPathDBiHostDB:ENSG00000160216.18
GeneCardsiAGPAT3
HGNCiHGNC:326 AGPAT3
MIMi614794 gene
neXtProtiNX_Q9NRZ7
OpenTargetsiENSG00000160216
PharmGKBiPA24623
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00890000139404
HOVERGENiHBG008205
InParanoidiQ9NRZ7
KOiK13523
OMAiFLFWATV
OrthoDBiEOG091G0C4Z
PhylomeDBiQ9NRZ7
TreeFamiTF314065

Enzyme and pathway databases

UniPathwayi
UPA00557;UER00613

BioCyciMetaCyc:HS08470-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-6811436 COPI-independent Golgi-to-ER retrograde traffic
SIGNORiQ9NRZ7

Miscellaneous databases

ChiTaRSiAGPAT3 human
GeneWikiiAGPAT3
GenomeRNAii56894
PROiPR:Q9NRZ7
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160216 Expressed in 204 organ(s), highest expression level in primary visual cortex
CleanExiHS_AGPAT3
ExpressionAtlasiQ9NRZ7 baseline and differential
GenevisibleiQ9NRZ7 HS

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCC_HUMAN
AccessioniPrimary (citable) accession number: Q9NRZ7
Secondary accession number(s): D3DSL2
, Q3ZCU2, Q6UWP6, Q6ZUC6, Q8N3Q7, Q9NRZ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
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