UniProtKB - Q9NRW4 (DUS22_HUMAN)
Protein
Dual specificity protein phosphatase 22
Gene
DUSP22
Organism
Homo sapiens (Human)
Status
Functioni
Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) (By similarity).By similarity1 Publication
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 88 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- non-membrane spanning protein tyrosine phosphatase activity Source: ARUK-UCL
- protein tyrosine/serine/threonine phosphatase activity Source: InterPro
- protein tyrosine kinase binding Source: ARUK-UCL
- protein tyrosine phosphatase activity Source: ARUK-UCL
GO - Biological processi
- cellular response to epidermal growth factor stimulus Source: ARUK-UCL
- negative regulation of cell migration Source: ARUK-UCL
- negative regulation of focal adhesion assembly Source: ARUK-UCL
- negative regulation of non-membrane spanning protein tyrosine kinase activity Source: ARUK-UCL
- negative regulation of T cell activation Source: Ensembl
- negative regulation of T cell mediated immunity Source: Ensembl
- negative regulation of T cell receptor signaling pathway Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: MGI
- peptidyl-tyrosine dephosphorylation Source: ARUK-UCL
- positive regulation of JNK cascade Source: GO_Central
- regulation of cell population proliferation Source: GO_Central
- transforming growth factor beta receptor signaling pathway Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Enzyme and pathway databases
PathwayCommonsi | Q9NRW4 |
SABIO-RKi | Q9NRW4 |
SignaLinki | Q9NRW4 |
SIGNORi | Q9NRW4 |
Names & Taxonomyi
Protein namesi | Recommended name: Dual specificity protein phosphatase 22 (EC:3.1.3.16, EC:3.1.3.48)Alternative name(s): JNK-stimulatory phosphatase-1 Short name: JSP-1 Low molecular weight dual specificity phosphatase 2 Short name: LMW-DSP2 Mitogen-activated protein kinase phosphatase x Short name: MAP kinase phosphatase x Short name: MKP-x |
Gene namesi | Name:DUSP22 Synonyms:JSP1, LMWDSP2, MKPX |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000112679.14 |
HGNCi | HGNC:16077, DUSP22 |
neXtProti | NX_Q9NRW4 |
Subcellular locationi
Cytoskeleton
- filamentous actin Source: ARUK-UCL
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: ARUK-UCL
Other locations
- cytoplasm Source: ARUK-UCL
- leading edge of lamellipodium Source: ARUK-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 56940 |
OpenTargetsi | ENSG00000112679 |
PharmGKBi | PA134991025 |
Miscellaneous databases
Pharosi | Q9NRW4, Tbio |
Chemistry databases
ChEMBLi | CHEMBL3924 |
Polymorphism and mutation databases
BioMutai | DUSP22 |
DMDMi | 74752929 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000244751 | 2 – 184 | Dual specificity protein phosphatase 22Add BLAST | 183 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Modified residuei | 58 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Myristoylation regulates subcellular location, and is necessary for activation of JNK.1 Publication
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
EPDi | Q9NRW4 |
jPOSTi | Q9NRW4 |
MassIVEi | Q9NRW4 |
MaxQBi | Q9NRW4 |
PaxDbi | Q9NRW4 |
PeptideAtlasi | Q9NRW4 |
PRIDEi | Q9NRW4 |
ProteomicsDBi | 82429 [Q9NRW4-1] 82430 [Q9NRW4-2] |
PTM databases
DEPODi | DUSP22 |
iPTMneti | Q9NRW4 |
PhosphoSitePlusi | Q9NRW4 |
Expressioni
Tissue specificityi
Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas.1 Publication
Gene expression databases
Bgeei | ENSG00000112679, Expressed in epithelium of mammary gland and 237 other tissues |
ExpressionAtlasi | Q9NRW4, baseline and differential |
Genevisiblei | Q9NRW4, HS |
Organism-specific databases
HPAi | ENSG00000112679, Low tissue specificity |
Interactioni
Subunit structurei
Monomer.
1 PublicationGO - Molecular functioni
- protein tyrosine kinase binding Source: ARUK-UCL
Protein-protein interaction databases
BioGRIDi | 121264, 142 interactors |
IntActi | Q9NRW4, 44 interactors |
STRINGi | 9606.ENSP00000397459 |
Chemistry databases
BindingDBi | Q9NRW4 |
Miscellaneous databases
RNActi | Q9NRW4, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9NRW4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NRW4 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 144 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 141 |
Sequence similaritiesi
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.Curated
Phylogenomic databases
eggNOGi | KOG1716, Eukaryota |
GeneTreei | ENSGT00940000157153 |
HOGENOMi | CLU_027074_5_1_1 |
InParanoidi | Q9NRW4 |
OMAi | TVRWIDD |
PhylomeDBi | Q9NRW4 |
TreeFami | TF105126 |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR020417, Atypical_DUSP IPR000340, Dual-sp_phosphatase_cat-dom IPR029021, Prot-tyrosine_phosphatase-like IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit |
PRINTSi | PR01908, ADSPHPHTASE |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NRW4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGNGMNKILP GLYIGNFKDA RDAEQLSKNK VTHILSVHDS ARPMLEGVKY
60 70 80 90 100
LCIPAADSPS QNLTRHFKES IKFIHECRLR GESCLVHCLA GVSRSVTLVI
110 120 130 140 150
AYIMTVTDFG WEDALHTVRA GRSCANPNVG FQRQLQEFEK HEVHQYRQWL
160 170 180
KEEYGESPLQ DAEEAKNILA APGILKFWAF LRRL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketS4R3A4 | S4R3A4_HUMAN | Dual-specificity protein phosphatas... | DUSP22 | 75 | Annotation score: | ||
S4R3M1 | S4R3M1_HUMAN | Dual-specificity protein phosphatas... | DUSP22 | 102 | Annotation score: | ||
S4R378 | S4R378_HUMAN | Dual-specificity protein phosphatas... | DUSP22 | 46 | Annotation score: | ||
S4R459 | S4R459_HUMAN | Dual-specificity protein phosphatas... | DUSP22 | 54 | Annotation score: | ||
S4R3K1 | S4R3K1_HUMAN | Dual-specificity protein phosphatas... | DUSP22 | 18 | Annotation score: |
Sequence cautioni
The sequence AAH16844 differs from that shown. Reason: Erroneous initiation.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_026912 | 119 | R → H. Corresponds to variant dbSNP:rs7768224Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_019614 | 170 – 184 | AAPGI…FLRRL → GKYKEQGRTEPQPGARRWSS FPALAPLTYDNYTTET in isoform 2. 2 PublicationsAdd BLAST | 15 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF424702 mRNA Translation: AAL18850.1 AF165519 mRNA Translation: AAF86649.1 AY249859 mRNA Translation: AAP76376.1 AK296402 mRNA Translation: BAG59068.1 AL365272 Genomic DNA No translation available. BC016844 mRNA Translation: AAH16844.1 Different initiation. BC022847 mRNA Translation: AAH22847.1 AB208997 mRNA Translation: BAD92234.1 |
CCDSi | CCDS4468.1 [Q9NRW4-1] CCDS69035.1 [Q9NRW4-2] |
RefSeqi | NP_001273484.1, NM_001286555.1 [Q9NRW4-2] NP_064570.1, NM_020185.4 [Q9NRW4-1] |
Genome annotation databases
Ensembli | ENST00000344450; ENSP00000345281; ENSG00000112679 [Q9NRW4-1] ENST00000419235; ENSP00000397459; ENSG00000112679 [Q9NRW4-2] |
GeneIDi | 56940 |
KEGGi | hsa:56940 |
UCSCi | uc003msx.5, human [Q9NRW4-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF424702 mRNA Translation: AAL18850.1 AF165519 mRNA Translation: AAF86649.1 AY249859 mRNA Translation: AAP76376.1 AK296402 mRNA Translation: BAG59068.1 AL365272 Genomic DNA No translation available. BC016844 mRNA Translation: AAH16844.1 Different initiation. BC022847 mRNA Translation: AAH22847.1 AB208997 mRNA Translation: BAD92234.1 |
CCDSi | CCDS4468.1 [Q9NRW4-1] CCDS69035.1 [Q9NRW4-2] |
RefSeqi | NP_001273484.1, NM_001286555.1 [Q9NRW4-2] NP_064570.1, NM_020185.4 [Q9NRW4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WRM | X-ray | 1.50 | A | 1-163 | [»] | |
4WOH | X-ray | 1.34 | A | 1-163 | [»] | |
SMRi | Q9NRW4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121264, 142 interactors |
IntActi | Q9NRW4, 44 interactors |
STRINGi | 9606.ENSP00000397459 |
Chemistry databases
BindingDBi | Q9NRW4 |
ChEMBLi | CHEMBL3924 |
PTM databases
DEPODi | DUSP22 |
iPTMneti | Q9NRW4 |
PhosphoSitePlusi | Q9NRW4 |
Polymorphism and mutation databases
BioMutai | DUSP22 |
DMDMi | 74752929 |
Proteomic databases
EPDi | Q9NRW4 |
jPOSTi | Q9NRW4 |
MassIVEi | Q9NRW4 |
MaxQBi | Q9NRW4 |
PaxDbi | Q9NRW4 |
PeptideAtlasi | Q9NRW4 |
PRIDEi | Q9NRW4 |
ProteomicsDBi | 82429 [Q9NRW4-1] 82430 [Q9NRW4-2] |
Protocols and materials databases
Antibodypediai | 9106, 301 antibodies |
DNASUi | 56940 |
Genome annotation databases
Ensembli | ENST00000344450; ENSP00000345281; ENSG00000112679 [Q9NRW4-1] ENST00000419235; ENSP00000397459; ENSG00000112679 [Q9NRW4-2] |
GeneIDi | 56940 |
KEGGi | hsa:56940 |
UCSCi | uc003msx.5, human [Q9NRW4-1] |
Organism-specific databases
CTDi | 56940 |
DisGeNETi | 56940 |
EuPathDBi | HostDB:ENSG00000112679.14 |
GeneCardsi | DUSP22 |
HGNCi | HGNC:16077, DUSP22 |
HPAi | ENSG00000112679, Low tissue specificity |
neXtProti | NX_Q9NRW4 |
OpenTargetsi | ENSG00000112679 |
PharmGKBi | PA134991025 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1716, Eukaryota |
GeneTreei | ENSGT00940000157153 |
HOGENOMi | CLU_027074_5_1_1 |
InParanoidi | Q9NRW4 |
OMAi | TVRWIDD |
PhylomeDBi | Q9NRW4 |
TreeFami | TF105126 |
Enzyme and pathway databases
PathwayCommonsi | Q9NRW4 |
SABIO-RKi | Q9NRW4 |
SignaLinki | Q9NRW4 |
SIGNORi | Q9NRW4 |
Miscellaneous databases
BioGRID-ORCSi | 56940, 18 hits in 850 CRISPR screens |
ChiTaRSi | DUSP22, human |
EvolutionaryTracei | Q9NRW4 |
GeneWikii | DUSP22 |
GenomeRNAii | 56940 |
Pharosi | Q9NRW4, Tbio |
PROi | PR:Q9NRW4 |
RNActi | Q9NRW4, protein |
Gene expression databases
Bgeei | ENSG00000112679, Expressed in epithelium of mammary gland and 237 other tissues |
ExpressionAtlasi | Q9NRW4, baseline and differential |
Genevisiblei | Q9NRW4, HS |
Family and domain databases
Gene3Di | 3.90.190.10, 1 hit |
InterProi | View protein in InterPro IPR020417, Atypical_DUSP IPR000340, Dual-sp_phosphatase_cat-dom IPR029021, Prot-tyrosine_phosphatase-like IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
Pfami | View protein in Pfam PF00782, DSPc, 1 hit |
PRINTSi | PR01908, ADSPHPHTASE |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit |
SUPFAMi | SSF52799, SSF52799, 1 hit |
PROSITEi | View protein in PROSITE PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DUS22_HUMAN | |
Accessioni | Q9NRW4Primary (citable) accession number: Q9NRW4 Secondary accession number(s): B4DK56 Q96AR1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2006 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations