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Protein

Phospholipid scramblase 4

Gene

PLSCR4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.

Cofactori

Ca2+1 Publication, Mg2+1 Publication

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • CD4 receptor binding Source: UniProtKB
  • enzyme binding Source: UniProtKB
  • phospholipid scramblase activity Source: GO_Central
  • SH3 domain binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

LigandCalcium

Enzyme and pathway databases

BRENDAi3.6.99.B1 2681

Protein family/group databases

TCDBi9.A.36.1.4 the ca(2+)-dependent phospholipid scramblase (scramblase) family

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid scramblase 4
Short name:
PL scramblase 4
Alternative name(s):
Ca(2+)-dependent phospholipid scramblase 4
Cell growth-inhibiting gene 43 protein
TRA1
Gene namesi
Name:PLSCR4
ORF Names:GIG43
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114698.14
HGNCiHGNC:16497 PLSCR4
MIMi607612 gene
neXtProtiNX_Q9NRQ2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 303CytoplasmicBy similarityAdd BLAST303
Transmembranei304 – 320HelicalSequence analysisAdd BLAST17
Topological domaini321 – 329ExtracellularBy similarity9

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi290D → A: 50% decrease in scramblase activity in presence of Ca2+, and 40% decrease in scramblase activity in presence of Mg2+. 1 Publication1

Organism-specific databases

DisGeNETi57088
OpenTargetsiENSG00000114698
PharmGKBiPA33422

Polymorphism and mutation databases

BioMutaiPLSCR4
DMDMi212276457

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007921 – 329Phospholipid scramblase 4Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83Phosphotyrosine; by ABLBy similarity1
Modified residuei88Phosphotyrosine; by ABLBy similarity1
Lipidationi197S-palmitoyl cysteineBy similarity1
Lipidationi198S-palmitoyl cysteineBy similarity1
Lipidationi199S-palmitoyl cysteineBy similarity1
Lipidationi201S-palmitoyl cysteineBy similarity1
Lipidationi202S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ9NRQ2
MaxQBiQ9NRQ2
PaxDbiQ9NRQ2
PeptideAtlasiQ9NRQ2
PRIDEiQ9NRQ2
ProteomicsDBi82403
82404 [Q9NRQ2-2]

PTM databases

iPTMnetiQ9NRQ2
PhosphoSitePlusiQ9NRQ2
SwissPalmiQ9NRQ2

Expressioni

Tissue specificityi

Expressed in heart, brain, placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, uterus, small intestine and colon. Not detected in peripheral blood lymphocytes.

Gene expression databases

BgeeiENSG00000114698 Expressed in 221 organ(s), highest expression level in germinal epithelium of ovary
CleanExiHS_PLSCR4
ExpressionAtlasiQ9NRQ2 baseline and differential
GenevisibleiQ9NRQ2 HS

Organism-specific databases

HPAiHPA002276

Interactioni

Subunit structurei

Interacts with PDCD6.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121357, 17 interactors
IntActiQ9NRQ2, 69 interactors
MINTiQ9NRQ2
STRINGi9606.ENSP00000347038

Structurei

3D structure databases

ProteinModelPortaliQ9NRQ2
SMRiQ9NRQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 98Proline-rich domain (PRD)By similarityAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi18 – 25SH3-binding 1Sequence analysis8
Motifi30 – 33PPxY motifSequence analysis4
Motifi41 – 49SH3-binding 2Sequence analysis9
Motifi98 – 106SH3-binding 3Sequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 202Cys-rich8

Domaini

The N-terminal proline-rich domain (PRD) is required for phospholipid scramblase activity.By similarity

Sequence similaritiesi

Belongs to the phospholipid scramblase family.Curated

Keywords - Domaini

Repeat, SH3-binding, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0621 Eukaryota
ENOG410XSYS LUCA
GeneTreeiENSGT00390000002884
HOGENOMiHOG000237356
HOVERGENiHBG019157
InParanoidiQ9NRQ2
OMAiDQMVYIV
OrthoDBiEOG091G0CUL
PhylomeDBiQ9NRQ2
TreeFamiTF314939

Family and domain databases

InterProiView protein in InterPro
IPR005552 Scramblase
PANTHERiPTHR23248 PTHR23248, 1 hit
PfamiView protein in Pfam
PF03803 Scramblase, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NRQ2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGVVPTAPE QPAGEMENQT KPPDPRPDAP PEYNSHFLPG PPGTAVPPPT
60 70 80 90 100
GYPGGLPMGY YSPQQPSTFP LYQPVGGIHP VRYQPGKYPM PNQSVPITWM
110 120 130 140 150
PGPTPMANCP PGLEYLVQLD NIHVLQHFEP LEMMTCFETN NRYDIKNNSD
160 170 180 190 200
QMVYIVTEDT DDFTRNAYRT LRPFVLRVTD CMGREIMTMQ RPFRCTCCCF
210 220 230 240 250
CCPSARQELE VQCPPGVTIG FVAEHWNLCR AVYSIQNEKK ENVMRVRGPC
260 270 280 290 300
STYGCGSDSV FEVKSLDGIS NIGSIIRKWN GLLSAMADAD HFDIHFPLDL
310 320
DVKMKAMIFG ACFLIDFMYF ERSPPQRSR
Length:329
Mass (Da):37,005
Last modified:November 4, 2008 - v2
Checksum:i12BE86728D54F794
GO
Isoform 2 (identifier: Q9NRQ2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.
     119-208: Missing.

Note: No experimental confirmation available.
Show »
Length:224
Mass (Da):24,797
Checksum:i55E36F0305DAB876
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PHR9E9PHR9_HUMAN
Phospholipid scramblase
PLSCR4
239Annotation score:
C9JNW2C9JNW2_HUMAN
Phospholipid scramblase
PLSCR4
147Annotation score:
C9J664C9J664_HUMAN
Phospholipid scramblase
PLSCR4
142Annotation score:
C9J6E1C9J6E1_HUMAN
Phospholipid scramblase
PLSCR4
262Annotation score:
C9J916C9J916_HUMAN
Phospholipid scramblase
PLSCR4
208Annotation score:
C9J3P9C9J3P9_HUMAN
Phospholipid scramblase 4
PLSCR4
97Annotation score:

Sequence cautioni

The sequence AAP97186 differs from that shown. Reason: Frameshift at positions 152, 155, 163 and 325.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56 – 57LP → FL in AAP97186 (Ref. 8) Curated2
Sequence conflicti74P → S in AAT52217 (Ref. 2) Curated1
Sequence conflicti149S → L in AAP97186 (Ref. 8) Curated1
Sequence conflicti163F → V in AAP97186 (Ref. 8) Curated1
Sequence conflicti175V → G in AAP97186 (Ref. 8) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01131534N → S2 PublicationsCorresponds to variant dbSNP:rs3762685Ensembl.1
Natural variantiVAR_011316155I → V2 PublicationsCorresponds to variant dbSNP:rs1061409Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0429311 – 15Missing in isoform 2. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_042932119 – 208Missing in isoform 2. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199023 mRNA Translation: AAF89960.1
AY550971 mRNA Translation: AAT52217.1
AK128442 mRNA Translation: BAC87442.1
AK290214 mRNA Translation: BAF82903.1
AL833760 mRNA Translation: CAH56232.1
AC092982 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW78935.1
BC028354 mRNA Translation: AAH28354.1
AF087887 mRNA Translation: AAP97186.1 Sequence problems.
CCDSiCCDS3133.1 [Q9NRQ2-1]
CCDS54651.1 [Q9NRQ2-2]
RefSeqiNP_001121776.1, NM_001128304.1 [Q9NRQ2-1]
NP_001121777.1, NM_001128305.1 [Q9NRQ2-1]
NP_001121778.1, NM_001128306.1
NP_001170775.1, NM_001177304.1 [Q9NRQ2-2]
NP_065086.2, NM_020353.2 [Q9NRQ2-1]
XP_005247711.1, XM_005247654.2 [Q9NRQ2-1]
XP_005247712.1, XM_005247655.2 [Q9NRQ2-1]
XP_011511333.1, XM_011513031.2 [Q9NRQ2-1]
UniGeneiHs.477869

Genome annotation databases

EnsembliENST00000354952; ENSP00000347038; ENSG00000114698 [Q9NRQ2-1]
ENST00000433593; ENSP00000415605; ENSG00000114698 [Q9NRQ2-2]
ENST00000446574; ENSP00000399315; ENSG00000114698 [Q9NRQ2-1]
ENST00000493382; ENSP00000419040; ENSG00000114698 [Q9NRQ2-1]
GeneIDi57088
KEGGihsa:57088
UCSCiuc003evt.6 human [Q9NRQ2-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199023 mRNA Translation: AAF89960.1
AY550971 mRNA Translation: AAT52217.1
AK128442 mRNA Translation: BAC87442.1
AK290214 mRNA Translation: BAF82903.1
AL833760 mRNA Translation: CAH56232.1
AC092982 Genomic DNA No translation available.
CH471052 Genomic DNA Translation: EAW78935.1
BC028354 mRNA Translation: AAH28354.1
AF087887 mRNA Translation: AAP97186.1 Sequence problems.
CCDSiCCDS3133.1 [Q9NRQ2-1]
CCDS54651.1 [Q9NRQ2-2]
RefSeqiNP_001121776.1, NM_001128304.1 [Q9NRQ2-1]
NP_001121777.1, NM_001128305.1 [Q9NRQ2-1]
NP_001121778.1, NM_001128306.1
NP_001170775.1, NM_001177304.1 [Q9NRQ2-2]
NP_065086.2, NM_020353.2 [Q9NRQ2-1]
XP_005247711.1, XM_005247654.2 [Q9NRQ2-1]
XP_005247712.1, XM_005247655.2 [Q9NRQ2-1]
XP_011511333.1, XM_011513031.2 [Q9NRQ2-1]
UniGeneiHs.477869

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3Q5UX-ray2.50B271-283[»]
ProteinModelPortaliQ9NRQ2
SMRiQ9NRQ2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121357, 17 interactors
IntActiQ9NRQ2, 69 interactors
MINTiQ9NRQ2
STRINGi9606.ENSP00000347038

Protein family/group databases

TCDBi9.A.36.1.4 the ca(2+)-dependent phospholipid scramblase (scramblase) family

PTM databases

iPTMnetiQ9NRQ2
PhosphoSitePlusiQ9NRQ2
SwissPalmiQ9NRQ2

Polymorphism and mutation databases

BioMutaiPLSCR4
DMDMi212276457

Proteomic databases

EPDiQ9NRQ2
MaxQBiQ9NRQ2
PaxDbiQ9NRQ2
PeptideAtlasiQ9NRQ2
PRIDEiQ9NRQ2
ProteomicsDBi82403
82404 [Q9NRQ2-2]

Protocols and materials databases

DNASUi57088
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354952; ENSP00000347038; ENSG00000114698 [Q9NRQ2-1]
ENST00000433593; ENSP00000415605; ENSG00000114698 [Q9NRQ2-2]
ENST00000446574; ENSP00000399315; ENSG00000114698 [Q9NRQ2-1]
ENST00000493382; ENSP00000419040; ENSG00000114698 [Q9NRQ2-1]
GeneIDi57088
KEGGihsa:57088
UCSCiuc003evt.6 human [Q9NRQ2-1]

Organism-specific databases

CTDi57088
DisGeNETi57088
EuPathDBiHostDB:ENSG00000114698.14
GeneCardsiPLSCR4
HGNCiHGNC:16497 PLSCR4
HPAiHPA002276
MIMi607612 gene
neXtProtiNX_Q9NRQ2
OpenTargetsiENSG00000114698
PharmGKBiPA33422
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0621 Eukaryota
ENOG410XSYS LUCA
GeneTreeiENSGT00390000002884
HOGENOMiHOG000237356
HOVERGENiHBG019157
InParanoidiQ9NRQ2
OMAiDQMVYIV
OrthoDBiEOG091G0CUL
PhylomeDBiQ9NRQ2
TreeFamiTF314939

Enzyme and pathway databases

BRENDAi3.6.99.B1 2681

Miscellaneous databases

GeneWikiiPLSCR4
GenomeRNAii57088
PROiPR:Q9NRQ2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000114698 Expressed in 221 organ(s), highest expression level in germinal epithelium of ovary
CleanExiHS_PLSCR4
ExpressionAtlasiQ9NRQ2 baseline and differential
GenevisibleiQ9NRQ2 HS

Family and domain databases

InterProiView protein in InterPro
IPR005552 Scramblase
PANTHERiPTHR23248 PTHR23248, 1 hit
PfamiView protein in Pfam
PF03803 Scramblase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLS4_HUMAN
AccessioniPrimary (citable) accession number: Q9NRQ2
Secondary accession number(s): A8K2E9
, Q2TTR3, Q658L3, Q6ZR73, Q7Z505
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: November 4, 2008
Last modified: November 7, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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