UniProtKB - Q9NRP7 (STK36_HUMAN)
Protein
Serine/threonine-protein kinase 36
Gene
STK36
Organism
Homo sapiens (Human)
Status
Functioni
Serine/threonine protein kinase which plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors (PubMed:10806483). Controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization (PubMed:10806483). GLI2 requires an additional function of STK36 to become transcriptionally active, but the enzyme does not need to possess an active kinase catalytic site for this to occur (PubMed:10806483). Required for postnatal development, possibly by regulating the homeostasis of cerebral spinal fluid or ciliary function (By similarity). Essential for construction of the central pair apparatus of motile cilia.By similarity1 Publication
Catalytic activityi
Cofactori
Mg2+Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | ATP | 1 | |
Active sitei | 125 | Proton acceptorPROSITE-ProRule annotationBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 10 – 18 | ATPPROSITE-ProRule annotationBy similarity | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein serine/threonine kinase activity Source: UniProtKB
- transcription factor binding Source: UniProtKB
GO - Biological processi
- brain development Source: Ensembl
- cilium assembly Source: UniProtKB
- epithelial cilium movement involved in extracellular fluid movement Source: Ensembl
- positive regulation of hh target transcription factor activity Source: UniProtKB
- positive regulation of smoothened signaling pathway Source: UniProtKB
- post-embryonic development Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- regulation of DNA-binding transcription factor activity Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cilium biogenesis/degradation |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
SignaLinki | Q9NRP7 |
SIGNORi | Q9NRP7 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17209 STK36 |
MIMi | 607652 gene |
neXtProti | NX_Q9NRP7 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular region Source: GOC
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 33 | K → R: No effect on nuclear localization of GLI1 or GLI2 or on GLI-mediated transcription. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 27148 |
OpenTargetsi | ENSG00000163482 |
Orphaneti | 244 Primary ciliary dyskinesia |
PharmGKBi | PA38212 |
Miscellaneous databases
Pharosi | Q9NRP7 |
Chemistry databases
ChEMBLi | CHEMBL4312 |
DrugBanki | DB12010 Fostamatinib |
DrugCentrali | Q9NRP7 |
Polymorphism and mutation databases
BioMutai | STK36 |
DMDMi | 90101761 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000229020 | 1 – 1315 | Serine/threonine-protein kinase 36Add BLAST | 1315 |
Proteomic databases
jPOSTi | Q9NRP7 |
MassIVEi | Q9NRP7 |
MaxQBi | Q9NRP7 |
PaxDbi | Q9NRP7 |
PeptideAtlasi | Q9NRP7 |
PRIDEi | Q9NRP7 |
ProteomicsDBi | 82401 [Q9NRP7-1] 82402 [Q9NRP7-2] |
PTM databases
iPTMneti | Q9NRP7 |
PhosphoSitePlusi | Q9NRP7 |
Expressioni
Tissue specificityi
Expressed at low levels in most fetal tissues, adult ovaries and at high levels in adult testis, where it is localized in germ cells.1 Publication
Gene expression databases
Bgeei | ENSG00000163482 Expressed in 163 organ(s), highest expression level in right uterine tube |
ExpressionAtlasi | Q9NRP7 baseline and differential |
Genevisiblei | Q9NRP7 HS |
Organism-specific databases
HPAi | HPA027409 HPA027453 HPA030058 |
Interactioni
Subunit structurei
Interacts with GLI1, GLI2 and GLI3 (PubMed:10806483).
Interacts with SPAG16 and KIF27.
By similarity1 PublicationBinary interactionsi
GO - Molecular functioni
- transcription factor binding Source: UniProtKB
Protein-protein interaction databases
BioGridi | 118032, 14 interactors |
IntActi | Q9NRP7, 8 interactors |
STRINGi | 9606.ENSP00000295709 |
Chemistry databases
BindingDBi | Q9NRP7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 254 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 251 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0597 Eukaryota ENOG410XRQ6 LUCA |
GeneTreei | ENSGT00940000158375 |
HOGENOMi | HOG000015284 |
InParanoidi | Q9NRP7 |
KOi | K06228 |
OMAi | VGLPGCW |
OrthoDBi | 979202at2759 |
PhylomeDBi | Q9NRP7 |
TreeFami | TF105340 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989 ARM-like IPR016024 ARM-type_fold IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069 Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220 S_TKc, 1 hit |
SUPFAMi | SSF48371 SSF48371, 2 hits SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 13 Publications (identifier: Q9NRP7-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEKYHVLEMI GEGSFGRVYK GRRKYSAQVV ALKFIPKLGR SEKELRNLQR
60 70 80 90 100
EIEIMRGLRH PNIVHMLDSF ETDKEVVVVT DYAEGELFQI LEDDGKLPED
110 120 130 140 150
QVQAIAAQLV SALYYLHSHR ILHRDMKPQN ILLAKGGGIK LCDFGFARAM
160 170 180 190 200
STNTMVLTSI KGTPLYMSPE LVEERPYDHT ADLWSVGCIL YELAVGTPPF
210 220 230 240 250
YATSIFQLVS LILKDPVRWP STISPCFKNF LQGLLTKDPR QRLSWPDLLY
260 270 280 290 300
HPFIAGHVTI ITEPAGPDLG TPFTSRLPPE LQVLKDEQAH RLAPKGNQSR
310 320 330 340 350
ILTQAYKRMA EEAMQKKHQN TGPALEQEDK TSKVAPGTAP LPRLGATPQE
360 370 380 390 400
SSLLAGILAS ELKSSWAKSG TGEVPSAPRE NRTTPDCERA FPEERPEVLG
410 420 430 440 450
QRSTDVVDLE NEEPDSDNEW QHLLETTEPV PIQLKAPLTL LCNPDFCQRI
460 470 480 490 500
QSQLHEAGGQ ILKGILEGAS HILPAFRVLS SLLSSCSDSV ALYSFCREAG
510 520 530 540 550
LPGLLLSLLR HSQESNSLQQ QSWYGTFLQD LMAVIQAYFA CTFNLERSQT
560 570 580 590 600
SDSLQVFQEA ANLFLDLLGK LLAQPDDSEQ TLRRDSLMCF TVLCEAMDGN
610 620 630 640 650
SRAISKAFYS SLLTTQQVVL DGLLHGLTVP QLPVHTPQGA PQVSQPLREQ
660 670 680 690 700
SEDIPGAISS ALAAICTAPV GLPDCWDAKE QVCWHLANQL TEDSSQLRPS
710 720 730 740 750
LISGLQHPIL CLHLLKVLYS CCLVSEGLCR LLGQEPLALE SLFMLIQGKV
760 770 780 790 800
KVVDWEESTE VTLYFLSLLV FRLQNLPCGM EKLGSDVATL FTHSHVVSLV
810 820 830 840 850
SAAACLLGQL GQQGVTFDLQ PMEWMAAATH ALSAPAEVRL TPPGSCGFYD
860 870 880 890 900
GLLILLLQLL TEQGKASLIR DMSSSEMWTV LWHRFSMVLR LPEEASAQEG
910 920 930 940 950
ELSLSSPPSP EPDWTLISPQ GMAALLSLAM ATFTQEPQLC LSCLSQHGSI
960 970 980 990 1000
LMSILKHLLC PSFLNQLRQA PHGSEFLPVV VLSVCQLLCF PFALDMDADL
1010 1020 1030 1040 1050
LIGVLADLRD SEVAAHLLQV CCYHLPLMQV ELPISLLTRL ALMDPTSLNQ
1060 1070 1080 1090 1100
FVNTVSASPR TIVSFLSVAL LSDQPLLTSD LLSLLAHTAR VLSPSHLSFI
1110 1120 1130 1140 1150
QELLAGSDES YRPLRSLLGH PENSVRAHTY RLLGHLLQHS MALRGALQSQ
1160 1170 1180 1190 1200
SGLLSLLLLG LGDKDPVVRC SASFAVGNAA YQAGPLGPAL AAAVPSMTQL
1210 1220 1230 1240 1250
LGDPQAGIRR NVASALGNLG PEGLGEELLQ CEVPQRLLEM ACGDPQPNVK
1260 1270 1280 1290 1300
EAALIALRSL QQEPGIHQVL VSLGASEKLS LLSLGNQSLP HSSPRPASAK
1310
HCRKLIHLLR PAHSM
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JDA4 | C9JDA4_HUMAN | Serine/threonine-protein kinase 36 | STK36 | 278 | Annotation score: | ||
H7C2I3 | H7C2I3_HUMAN | Serine/threonine-protein kinase 36 | STK36 | 106 | Annotation score: | ||
C9J1B8 | C9J1B8_HUMAN | Serine/threonine-protein kinase 36 | STK36 | 75 | Annotation score: | ||
H7C2A4 | H7C2A4_HUMAN | Serine/threonine-protein kinase 36 | STK36 | 148 | Annotation score: | ||
H7C359 | H7C359_HUMAN | Serine/threonine-protein kinase 36 | STK36 | 66 | Annotation score: |
Sequence cautioni
The sequence BAA86592 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14184 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 229 | N → D in AAH26158 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 971 – 986 | Missing (PubMed:14702039).CuratedAdd BLAST | 16 | |
Sequence conflicti | 1003 | G → V in AAF97028 (PubMed:10806483).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_041177 | 90 | I → M1 PublicationCorresponds to variant dbSNP:rs55706732Ensembl. | 1 | |
Natural variantiVAR_041178 | 240 | R → W1 PublicationCorresponds to variant dbSNP:rs35038757Ensembl. | 1 | |
Natural variantiVAR_041179 | 295 | K → R1 PublicationCorresponds to variant dbSNP:rs1863703Ensembl. | 1 | |
Natural variantiVAR_041180 | 329 | D → N1 PublicationCorresponds to variant dbSNP:rs34027859Ensembl. | 1 | |
Natural variantiVAR_041181 | 462 | L → V1 PublicationCorresponds to variant dbSNP:rs45586733Ensembl. | 1 | |
Natural variantiVAR_025727 | 463 | K → N1 PublicationCorresponds to variant dbSNP:rs17856747Ensembl. | 1 | |
Natural variantiVAR_041182 | 476 | F → S1 PublicationCorresponds to variant dbSNP:rs34128793Ensembl. | 1 | |
Natural variantiVAR_041183 | 477 | R → W1 PublicationCorresponds to variant dbSNP:rs16859180Ensembl. | 1 | |
Natural variantiVAR_041184 | 583 | R → Q1 PublicationCorresponds to variant dbSNP:rs1344642EnsemblClinVar. | 1 | |
Natural variantiVAR_057112 | 638 | Q → P. Corresponds to variant dbSNP:rs6709303Ensembl. | 1 | |
Natural variantiVAR_041185 | 660 | S → C in a breast pleomorphic lobular carcinoma sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs1244829273Ensembl. | 1 | |
Natural variantiVAR_041186 | 672 | L → P1 PublicationCorresponds to variant dbSNP:rs35448374Ensembl. | 1 | |
Natural variantiVAR_025728 | 767 | S → T1 PublicationCorresponds to variant dbSNP:rs17856748Ensembl. | 1 | |
Natural variantiVAR_041187 | 767 | S → Y in an ovarian papillary serous adenocarcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_041188 | 816 | T → A1 PublicationCorresponds to variant dbSNP:rs34271431Ensembl. | 1 | |
Natural variantiVAR_041189 | 839 | R → Q1 PublicationCorresponds to variant dbSNP:rs13023540Ensembl. | 1 | |
Natural variantiVAR_041190 | 840 | L → V1 PublicationCorresponds to variant dbSNP:rs36099639Ensembl. | 1 | |
Natural variantiVAR_025729 | 1003 | G → D2 PublicationsCorresponds to variant dbSNP:rs1863704EnsemblClinVar. | 1 | |
Natural variantiVAR_061747 | 1004 | V → I. Corresponds to variant dbSNP:rs55633575Ensembl. | 1 | |
Natural variantiVAR_041191 | 1111 | Y → C1 PublicationCorresponds to variant dbSNP:rs56278660Ensembl. | 1 | |
Natural variantiVAR_041192 | 1112 | R → Q1 PublicationCorresponds to variant dbSNP:rs12993599Ensembl. | 1 | |
Natural variantiVAR_041193 | 1138 | Q → K in an ovarian serous carcinoma sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_041194 | 1185 | P → S in an ovarian endometrioid sample; somatic mutation. 1 Publication | 1 | |
Natural variantiVAR_041195 | 1313 | H → P1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_051983 | 838 – 858 | Missing in isoform 2. 1 PublicationAdd BLAST | 21 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF200815 mRNA Translation: AAF97028.1 AB033104 mRNA Translation: BAA86592.1 Different initiation. AC009974 Genomic DNA No translation available. BC026158 mRNA Translation: AAH26158.1 AL133630 mRNA Translation: CAB63754.1 AK022692 mRNA Translation: BAB14184.1 Frameshift. |
CCDSi | CCDS2421.1 [Q9NRP7-1] CCDS58750.1 [Q9NRP7-2] |
PIRi | T43465 |
RefSeqi | NP_001230242.1, NM_001243313.1 [Q9NRP7-2] NP_056505.2, NM_015690.4 [Q9NRP7-1] XP_005246521.1, XM_005246464.1 XP_016859293.1, XM_017003804.1 [Q9NRP7-2] |
Genome annotation databases
Ensembli | ENST00000295709; ENSP00000295709; ENSG00000163482 [Q9NRP7-1] ENST00000392105; ENSP00000375954; ENSG00000163482 [Q9NRP7-2] ENST00000440309; ENSP00000394095; ENSG00000163482 [Q9NRP7-1] |
GeneIDi | 27148 |
KEGGi | hsa:27148 |
UCSCi | uc002viu.4 human [Q9NRP7-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF200815 mRNA Translation: AAF97028.1 AB033104 mRNA Translation: BAA86592.1 Different initiation. AC009974 Genomic DNA No translation available. BC026158 mRNA Translation: AAH26158.1 AL133630 mRNA Translation: CAB63754.1 AK022692 mRNA Translation: BAB14184.1 Frameshift. |
CCDSi | CCDS2421.1 [Q9NRP7-1] CCDS58750.1 [Q9NRP7-2] |
PIRi | T43465 |
RefSeqi | NP_001230242.1, NM_001243313.1 [Q9NRP7-2] NP_056505.2, NM_015690.4 [Q9NRP7-1] XP_005246521.1, XM_005246464.1 XP_016859293.1, XM_017003804.1 [Q9NRP7-2] |
3D structure databases
SMRi | Q9NRP7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 118032, 14 interactors |
IntActi | Q9NRP7, 8 interactors |
STRINGi | 9606.ENSP00000295709 |
Chemistry databases
BindingDBi | Q9NRP7 |
ChEMBLi | CHEMBL4312 |
DrugBanki | DB12010 Fostamatinib |
DrugCentrali | Q9NRP7 |
PTM databases
iPTMneti | Q9NRP7 |
PhosphoSitePlusi | Q9NRP7 |
Polymorphism and mutation databases
BioMutai | STK36 |
DMDMi | 90101761 |
Proteomic databases
jPOSTi | Q9NRP7 |
MassIVEi | Q9NRP7 |
MaxQBi | Q9NRP7 |
PaxDbi | Q9NRP7 |
PeptideAtlasi | Q9NRP7 |
PRIDEi | Q9NRP7 |
ProteomicsDBi | 82401 [Q9NRP7-1] 82402 [Q9NRP7-2] |
Protocols and materials databases
DNASUi | 27148 |
Genome annotation databases
Ensembli | ENST00000295709; ENSP00000295709; ENSG00000163482 [Q9NRP7-1] ENST00000392105; ENSP00000375954; ENSG00000163482 [Q9NRP7-2] ENST00000440309; ENSP00000394095; ENSG00000163482 [Q9NRP7-1] |
GeneIDi | 27148 |
KEGGi | hsa:27148 |
UCSCi | uc002viu.4 human [Q9NRP7-1] |
Organism-specific databases
CTDi | 27148 |
DisGeNETi | 27148 |
GeneCardsi | STK36 |
HGNCi | HGNC:17209 STK36 |
HPAi | HPA027409 HPA027453 HPA030058 |
MIMi | 607652 gene |
neXtProti | NX_Q9NRP7 |
OpenTargetsi | ENSG00000163482 |
Orphaneti | 244 Primary ciliary dyskinesia |
PharmGKBi | PA38212 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0597 Eukaryota ENOG410XRQ6 LUCA |
GeneTreei | ENSGT00940000158375 |
HOGENOMi | HOG000015284 |
InParanoidi | Q9NRP7 |
KOi | K06228 |
OMAi | VGLPGCW |
OrthoDBi | 979202at2759 |
PhylomeDBi | Q9NRP7 |
TreeFami | TF105340 |
Enzyme and pathway databases
SignaLinki | Q9NRP7 |
SIGNORi | Q9NRP7 |
Miscellaneous databases
ChiTaRSi | STK36 human |
GeneWikii | STK36 |
GenomeRNAii | 27148 |
Pharosi | Q9NRP7 |
PROi | PR:Q9NRP7 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163482 Expressed in 163 organ(s), highest expression level in right uterine tube |
ExpressionAtlasi | Q9NRP7 baseline and differential |
Genevisiblei | Q9NRP7 HS |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989 ARM-like IPR016024 ARM-type_fold IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR008271 Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069 Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220 S_TKc, 1 hit |
SUPFAMi | SSF48371 SSF48371, 2 hits SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00108 PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STK36_HUMAN | |
Accessioni | Q9NRP7Primary (citable) accession number: Q9NRP7 Secondary accession number(s): B7WPM3 Q9ULE2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | March 21, 2006 | |
Last modified: | October 16, 2019 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations - Human and mouse protein kinases
Human and mouse protein kinases: classification and index - SIMILARITY comments
Index of protein domains and families - Human chromosome 2
Human chromosome 2: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot