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Protein

DNA polymerase epsilon subunit 3

Gene

POLE3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: ProtInc
  • protein heterodimerization activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-174430 Telomere C-strand synthesis initiation
R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-68952 DNA replication initiation
R-HSA-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon subunit 3 (EC:2.7.7.7)
Alternative name(s):
Arsenic-transactivated protein
Short name:
AsTP
Chromatin accessibility complex 17 kDa protein
Short name:
CHRAC-17
Short name:
HuCHRAC17
DNA polymerase II subunit 3
DNA polymerase epsilon subunit p17
Gene namesi
Name:POLE3
Synonyms:CHRAC17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000148229.12
HGNCiHGNC:13546 POLE3
MIMi607267 gene
neXtProtiNX_Q9NRF9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi54107
OpenTargetsiENSG00000148229
PharmGKBiPA33499

Chemistry databases

ChEMBLiCHEMBL2363042
DrugBankiDB00242 Cladribine

Polymorphism and mutation databases

BioMutaiPOLE3
DMDMi22653710

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00002083412 – 147DNA polymerase epsilon subunit 3Add BLAST146

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Modified residuei83PhosphothreonineCombined sources1
Modified residuei122PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NRF9
MaxQBiQ9NRF9
PaxDbiQ9NRF9
PeptideAtlasiQ9NRF9
PRIDEiQ9NRF9
ProteomicsDBi82350
TopDownProteomicsiQ9NRF9

PTM databases

iPTMnetiQ9NRF9
PhosphoSitePlusiQ9NRF9
SwissPalmiQ9NRF9

Expressioni

Tissue specificityi

Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Gene expression databases

BgeeiENSG00000148229 Expressed in 226 organ(s), highest expression level in testis
CleanExiHS_POLE3
ExpressionAtlasiQ9NRF9 baseline and differential
GenevisibleiQ9NRF9 HS

Organism-specific databases

HPAiHPA047945

Interactioni

Subunit structurei

Component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interaction with POLE4 is a prerequisite for further binding with POLE and POLE2. Interacts with CHRAC1. Together with CHRAC1, ACF1 and ISWI/SNF2H proteins, it forms the ISWI chromatin-remodeling complex, CHRAC.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi119903, 43 interactors
ComplexPortaliCPX-2108 epsilon DNA polymerase complex
CPX-785 CHRAC chromatin remodeling complex
CORUMiQ9NRF9
IntActiQ9NRF9, 11 interactors
MINTiQ9NRF9
STRINGi9606.ENSP00000363284

Structurei

3D structure databases

ProteinModelPortaliQ9NRF9
SMRiQ9NRF9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili85 – 146Sequence analysisAdd BLAST62

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0870 Eukaryota
ENOG4111QSW LUCA
GeneTreeiENSGT00550000074689
HOGENOMiHOG000007701
HOVERGENiHBG060262
InParanoidiQ9NRF9
KOiK02326
OMAiCANNFAL
OrthoDBiEOG091G0Y4D
PhylomeDBiQ9NRF9
TreeFamiTF103008

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NRF9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAERPEDLNL PNAVITRIIK EALPDGVNIS KEARSAISRA ASVFVLYATS
60 70 80 90 100
CANNFAMKGK RKTLNASDVL SAMEEMEFQR FVTPLKEALE AYRREQKGKK
110 120 130 140
EASEQKKKDK DKKTDSEEQD KSRDEDNDED EERLEEEEQN EEEEVDN
Length:147
Mass (Da):16,860
Last modified:October 1, 2000 - v1
Checksum:iDC0A4D2097046569
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti58K → N in AAU15052 (Ref. 3) Curated1
Sequence conflicti58K → N in BAC11206 (PubMed:14702039).Curated1
Sequence conflicti83T → I in BAC11190 (PubMed:14702039).Curated1
Sequence conflicti146D → Y in AAF90133 (PubMed:10801849).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02346483T → A1 PublicationCorresponds to variant dbSNP:rs36023979Ensembl.1
Natural variantiVAR_057527126D → A. Corresponds to variant dbSNP:rs34852828Ensembl.1
Natural variantiVAR_023465135E → D1 PublicationCorresponds to variant dbSNP:rs35933626Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226077 mRNA Translation: AAF72417.1
AF261689 Genomic DNA Translation: AAF90133.1
AY720898 mRNA Translation: AAU15052.1
AK074629 mRNA Translation: BAC11099.1
AK074762 mRNA Translation: BAC11190.1
AK074782 mRNA Translation: BAC11206.1
DQ072116 Genomic DNA Translation: AAY57326.1
AL137066 Genomic DNA No translation available.
BC003166 mRNA Translation: AAH03166.1
BC004170 mRNA Translation: AAH04170.1
CCDSiCCDS6795.1
RefSeqiNP_001265184.1, NM_001278255.1
NP_059139.3, NM_017443.4
UniGeneiHs.108112

Genome annotation databases

EnsembliENST00000374169; ENSP00000363284; ENSG00000148229
ENST00000374171; ENSP00000363286; ENSG00000148229
GeneIDi54107
KEGGihsa:54107
UCSCiuc004bhn.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF226077 mRNA Translation: AAF72417.1
AF261689 Genomic DNA Translation: AAF90133.1
AY720898 mRNA Translation: AAU15052.1
AK074629 mRNA Translation: BAC11099.1
AK074762 mRNA Translation: BAC11190.1
AK074782 mRNA Translation: BAC11206.1
DQ072116 Genomic DNA Translation: AAY57326.1
AL137066 Genomic DNA No translation available.
BC003166 mRNA Translation: AAH03166.1
BC004170 mRNA Translation: AAH04170.1
CCDSiCCDS6795.1
RefSeqiNP_001265184.1, NM_001278255.1
NP_059139.3, NM_017443.4
UniGeneiHs.108112

3D structure databases

ProteinModelPortaliQ9NRF9
SMRiQ9NRF9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119903, 43 interactors
ComplexPortaliCPX-2108 epsilon DNA polymerase complex
CPX-785 CHRAC chromatin remodeling complex
CORUMiQ9NRF9
IntActiQ9NRF9, 11 interactors
MINTiQ9NRF9
STRINGi9606.ENSP00000363284

Chemistry databases

ChEMBLiCHEMBL2363042
DrugBankiDB00242 Cladribine

PTM databases

iPTMnetiQ9NRF9
PhosphoSitePlusiQ9NRF9
SwissPalmiQ9NRF9

Polymorphism and mutation databases

BioMutaiPOLE3
DMDMi22653710

Proteomic databases

EPDiQ9NRF9
MaxQBiQ9NRF9
PaxDbiQ9NRF9
PeptideAtlasiQ9NRF9
PRIDEiQ9NRF9
ProteomicsDBi82350
TopDownProteomicsiQ9NRF9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374169; ENSP00000363284; ENSG00000148229
ENST00000374171; ENSP00000363286; ENSG00000148229
GeneIDi54107
KEGGihsa:54107
UCSCiuc004bhn.4 human

Organism-specific databases

CTDi54107
DisGeNETi54107
EuPathDBiHostDB:ENSG00000148229.12
GeneCardsiPOLE3
HGNCiHGNC:13546 POLE3
HPAiHPA047945
MIMi607267 gene
neXtProtiNX_Q9NRF9
OpenTargetsiENSG00000148229
PharmGKBiPA33499
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0870 Eukaryota
ENOG4111QSW LUCA
GeneTreeiENSGT00550000074689
HOGENOMiHOG000007701
HOVERGENiHBG060262
InParanoidiQ9NRF9
KOiK02326
OMAiCANNFAL
OrthoDBiEOG091G0Y4D
PhylomeDBiQ9NRF9
TreeFamiTF103008

Enzyme and pathway databases

ReactomeiR-HSA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-HSA-174430 Telomere C-strand synthesis initiation
R-HSA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-5685942 HDR through Homologous Recombination (HRR)
R-HSA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-HSA-5696400 Dual Incision in GG-NER
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-68952 DNA replication initiation
R-HSA-68962 Activation of the pre-replicative complex

Miscellaneous databases

ChiTaRSiPOLE3 human
GeneWikiiPOLE3
GenomeRNAii54107
PROiPR:Q9NRF9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148229 Expressed in 226 organ(s), highest expression level in testis
CleanExiHS_POLE3
ExpressionAtlasiQ9NRF9 baseline and differential
GenevisibleiQ9NRF9 HS

Family and domain databases

Gene3Di1.10.20.10, 1 hit
InterProiView protein in InterPro
IPR003958 CBFA_NFYB_domain
IPR009072 Histone-fold
PfamiView protein in Pfam
PF00808 CBFD_NFYB_HMF, 1 hit
SUPFAMiSSF47113 SSF47113, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPOE3_HUMAN
AccessioniPrimary (citable) accession number: Q9NRF9
Secondary accession number(s): Q5W0U1
, Q8N758, Q8NCE5, Q9NR32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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