UniProtKB - Q9NRD5 (PICK1_HUMAN)
PRKCA-binding protein
PICK1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | ZincBy similarity | 1 | |
Metal bindingi | 46 | ZincBy similarity | 1 |
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- Arp2/3 complex binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- G protein-coupled receptor binding Source: ParkinsonsUK-UCL
- identical protein binding Source: IntAct
- membrane curvature sensor activity Source: FlyBase
- metal ion binding Source: UniProtKB-KW
- phospholipid binding Source: GO_Central
- protein C-terminus binding Source: UniProtKB
- protein domain specific binding Source: ParkinsonsUK-UCL
- protein kinase C binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
GO - Biological processi
- cellular response to decreased oxygen levels Source: UniProtKB
- cellular response to glucose starvation Source: UniProtKB
- dendritic spine maintenance Source: UniProtKB
- dendritic spine organization Source: UniProtKB
- DNA methylation involved in embryo development Source: UniProtKB
- DNA methylation involved in gamete generation Source: UniProtKB
- glial cell development Source: UniProtKB
- intracellular protein transport Source: GO_Central
- long-term synaptic depression Source: UniProtKB
- monoamine transport Source: UniProtKB
- negative regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
- neuronal ion channel clustering Source: UniProtKB
- positive regulation of receptor internalization Source: UniProtKB
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- receptor clustering Source: UniProtKB
- regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
- regulation of insulin secretion Source: FlyBase
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: UniProtKB
Keywordsi
Molecular function | Actin-binding |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9NRD5 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-416993, Trafficking of GluR2-containing AMPA receptors |
SignaLinki | Q9NRD5 |
SIGNORi | Q9NRD5 |
Protein family/group databases
MoonDBi | Q9NRD5, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: PRKCA-binding proteinAlternative name(s): Protein interacting with C kinase 1 Protein kinase C-alpha-binding protein |
Gene namesi | Name:PICK1 Synonyms:PRKCABP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000100151.15 |
HGNCi | HGNC:9394, PICK1 |
MIMi | 605926, gene |
neXtProti | NX_Q9NRD5 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Other locations
- perinuclear region
- Membrane By similarity; Peripheral membrane protein By similarity
- Membrane By similarity; Lipid-anchor By similarity
- postsynaptic density By similarity
- synaptosome By similarity
Note: Also membrane-associated, present at excitatory synapses.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: HPA
Endosome
- postsynaptic early endosome Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- trans-Golgi network membrane Source: GO_Central
Mitochondrion
- mitochondrion Source: HPA
Plasma Membrane
- plasma membrane Source: UniProtKB
- presynaptic membrane Source: UniProtKB
Other locations
- aggresome Source: HPA
- cytoplasm Source: UniProtKB
- endocytic vesicle membrane Source: Reactome
- neuron projection Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- postsynaptic density Source: GO_Central
- synapse Source: UniProtKB
- synaptic vesicle Source: GO_Central
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoskeleton, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 – 28 | KD → AA: Abolishes interaction with other proteins, but not with itself. 3 Publications | 2 | |
Mutagenesisi | 27 | K → E: Abolishes interaction with GRIA2, but not with PRKCA. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9463 |
MalaCardsi | PICK1 |
OpenTargetsi | ENSG00000100151 |
Orphaneti | 171709, Male infertility due to globozoospermia |
PharmGKBi | PA33760 |
Miscellaneous databases
Pharosi | Q9NRD5, Tbio |
Polymorphism and mutation databases
BioMutai | PICK1 |
DMDMi | 22095990 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058427 | 1 – 415 | PRKCA-binding proteinAdd BLAST | 415 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | Phosphothreonine1 Publication | 1 | |
Lipidationi | 413 | S-palmitoyl cysteine; by DHHC8By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
EPDi | Q9NRD5 |
jPOSTi | Q9NRD5 |
MassIVEi | Q9NRD5 |
MaxQBi | Q9NRD5 |
PaxDbi | Q9NRD5 |
PeptideAtlasi | Q9NRD5 |
PRIDEi | Q9NRD5 |
ProteomicsDBi | 82339 [Q9NRD5-1] |
PTM databases
iPTMneti | Q9NRD5 |
PhosphoSitePlusi | Q9NRD5 |
SwissPalmi | Q9NRD5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000100151, Expressed in adenohypophysis and 172 other tissues |
ExpressionAtlasi | Q9NRD5, baseline and differential |
Genevisiblei | Q9NRD5, HS |
Organism-specific databases
HPAi | ENSG00000100151, Low tissue specificity |
Interactioni
Subunit structurei
Monomer and homodimer.
Interacts with CXADR.
Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2.
Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR.
Interacts with UNC5A.
Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner.
Interacts with F-actin and associates with the ARP2/3 complex.
Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition.
6 PublicationsBinary interactionsi
Q9NRD5
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- enzyme binding Source: UniProtKB
- G protein-coupled receptor binding Source: ParkinsonsUK-UCL
- identical protein binding Source: IntAct
- protein C-terminus binding Source: UniProtKB
- protein domain specific binding Source: ParkinsonsUK-UCL
- protein kinase C binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114849, 389 interactors |
CORUMi | Q9NRD5 |
IntActi | Q9NRD5, 378 interactors |
MINTi | Q9NRD5 |
STRINGi | 9606.ENSP00000385205 |
Miscellaneous databases
RNActi | Q9NRD5, protein |
Structurei
Secondary structure
3D structure databases
SASBDBi | Q9NRD5 |
SMRi | Q9NRD5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NRD5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 105 | PDZPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 144 – 357 | AHPROSITE-ProRule annotationAdd BLAST | 214 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 382 – 388 | Poly-Glu | 7 |
Domaini
Phylogenomic databases
eggNOGi | KOG3651, Eukaryota |
GeneTreei | ENSGT00950000183040 |
HOGENOMi | CLU_032347_1_0_1 |
InParanoidi | Q9NRD5 |
OMAi | FTQISAH |
PhylomeDBi | Q9NRD5 |
TreeFami | TF314945 |
Family and domain databases
CDDi | cd07659, BAR_PICK1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR010504, AH_dom IPR030798, Arfaptin_fam IPR001478, PDZ IPR036034, PDZ_sf IPR037959, PICK1_BAR |
PANTHERi | PTHR12141, PTHR12141, 1 hit |
Pfami | View protein in Pfam PF06456, Arfaptin, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM01015, Arfaptin, 1 hit SM00228, PDZ, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50870, AH, 1 hit PS50106, PDZ, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV
60 70 80 90 100
QVFDNTPAAL DGTVAAGDEI TGVNGRSIKG KTKVEVAKMI QEVKGEVTIH
110 120 130 140 150
YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV
160 170 180 190 200
KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ
210 220 230 240 250
PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI
260 270 280 290 300
KKYLDVKFEY LSYCLKVKEM DDEEYSCIAL GEPLYRVSTG NYEYRLILRC
310 320 330 340 350
RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRLVST MSKYYNDCYA
360 370 380 390 400
VLRDADVFPI EVDLAHTTLA YGLNQEEFTD GEEEEEEEDT AAGEPSRDTR
410
GAAGPLDKGG SWCDS
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6V107 | F6V107_HUMAN | PRKCA-binding protein | PICK1 | 229 | Annotation score: | ||
F6V3V1 | F6V3V1_HUMAN | PRKCA-binding protein | PICK1 | 130 | Annotation score: | ||
F6VY12 | F6VY12_HUMAN | PRKCA-binding protein | PICK1 | 134 | Annotation score: | ||
F6TII1 | F6TII1_HUMAN | PRKCA-binding protein | PICK1 | 38 | Annotation score: | ||
E7EX80 | E7EX80_HUMAN | PRKCA-binding protein | PICK1 | 51 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 7 | Y → F in AAF97502 (Ref. 2) Curated | 1 | |
Sequence conflicti | 16 | Missing in AAF97502 (Ref. 2) Curated | 1 | |
Sequence conflicti | 236 | N → NI in AAF97502 (Ref. 2) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054902 | 279 – 364 | ALGEP…IEVDL → VSVGGGGGLVLPPTWSARDS RWLRPTRERPGGLGWTLVPG DLGPSSQSRSLGLGVLGTRP GTTPSVWGTLGGKSDFLHSY EALLSV in isoform 2. 1 PublicationAdd BLAST | 86 | |
Alternative sequenceiVSP_054903 | 365 – 415 | Missing in isoform 2. 1 PublicationAdd BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB026491 mRNA Translation: BAA89294.1 AF231710 mRNA Translation: AAF97502.1 AL049654 mRNA Translation: CAB41082.1 CR456550 mRNA Translation: CAG30436.1 AK092818 mRNA Translation: BAG52614.1 AL031587 Genomic DNA No translation available. CH471095 Genomic DNA Translation: EAW60208.1 BC017561 mRNA Translation: AAH17561.1 |
CCDSi | CCDS13965.1 [Q9NRD5-1] |
PIRi | JC7167 |
RefSeqi | NP_001034672.1, NM_001039583.1 [Q9NRD5-1] NP_001034673.1, NM_001039584.1 [Q9NRD5-1] NP_036539.1, NM_012407.3 [Q9NRD5-1] |
Genome annotation databases
Ensembli | ENST00000356976; ENSP00000349465; ENSG00000100151 [Q9NRD5-1] ENST00000404072; ENSP00000385205; ENSG00000100151 [Q9NRD5-1] |
GeneIDi | 9463 |
KEGGi | hsa:9463 |
UCSCi | uc003auq.4, human [Q9NRD5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB026491 mRNA Translation: BAA89294.1 AF231710 mRNA Translation: AAF97502.1 AL049654 mRNA Translation: CAB41082.1 CR456550 mRNA Translation: CAG30436.1 AK092818 mRNA Translation: BAG52614.1 AL031587 Genomic DNA No translation available. CH471095 Genomic DNA Translation: EAW60208.1 BC017561 mRNA Translation: AAH17561.1 |
CCDSi | CCDS13965.1 [Q9NRD5-1] |
PIRi | JC7167 |
RefSeqi | NP_001034672.1, NM_001039583.1 [Q9NRD5-1] NP_001034673.1, NM_001039584.1 [Q9NRD5-1] NP_036539.1, NM_012407.3 [Q9NRD5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GZV | X-ray | 1.12 | A | 19-105 | [»] | |
6AR4 | X-ray | 1.69 | A/B | 1-105 | [»] | |
6BJN | X-ray | 2.43 | A/B | 1-105 | [»] | |
6BJO | X-ray | 1.75 | A/B | 1-105 | [»] | |
SASBDBi | Q9NRD5 | |||||
SMRi | Q9NRD5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114849, 389 interactors |
CORUMi | Q9NRD5 |
IntActi | Q9NRD5, 378 interactors |
MINTi | Q9NRD5 |
STRINGi | 9606.ENSP00000385205 |
Protein family/group databases
MoonDBi | Q9NRD5, Predicted |
PTM databases
iPTMneti | Q9NRD5 |
PhosphoSitePlusi | Q9NRD5 |
SwissPalmi | Q9NRD5 |
Polymorphism and mutation databases
BioMutai | PICK1 |
DMDMi | 22095990 |
Proteomic databases
EPDi | Q9NRD5 |
jPOSTi | Q9NRD5 |
MassIVEi | Q9NRD5 |
MaxQBi | Q9NRD5 |
PaxDbi | Q9NRD5 |
PeptideAtlasi | Q9NRD5 |
PRIDEi | Q9NRD5 |
ProteomicsDBi | 82339 [Q9NRD5-1] |
Protocols and materials databases
ABCDi | Q9NRD5, 1 sequenced antibody |
Antibodypediai | 12276, 432 antibodies |
DNASUi | 9463 |
Genome annotation databases
Ensembli | ENST00000356976; ENSP00000349465; ENSG00000100151 [Q9NRD5-1] ENST00000404072; ENSP00000385205; ENSG00000100151 [Q9NRD5-1] |
GeneIDi | 9463 |
KEGGi | hsa:9463 |
UCSCi | uc003auq.4, human [Q9NRD5-1] |
Organism-specific databases
CTDi | 9463 |
DisGeNETi | 9463 |
EuPathDBi | HostDB:ENSG00000100151.15 |
GeneCardsi | PICK1 |
HGNCi | HGNC:9394, PICK1 |
HPAi | ENSG00000100151, Low tissue specificity |
MalaCardsi | PICK1 |
MIMi | 605926, gene |
neXtProti | NX_Q9NRD5 |
OpenTargetsi | ENSG00000100151 |
Orphaneti | 171709, Male infertility due to globozoospermia |
PharmGKBi | PA33760 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3651, Eukaryota |
GeneTreei | ENSGT00950000183040 |
HOGENOMi | CLU_032347_1_0_1 |
InParanoidi | Q9NRD5 |
OMAi | FTQISAH |
PhylomeDBi | Q9NRD5 |
TreeFami | TF314945 |
Enzyme and pathway databases
PathwayCommonsi | Q9NRD5 |
Reactomei | R-HSA-202733, Cell surface interactions at the vascular wall R-HSA-416993, Trafficking of GluR2-containing AMPA receptors |
SignaLinki | Q9NRD5 |
SIGNORi | Q9NRD5 |
Miscellaneous databases
BioGRID-ORCSi | 9463, 4 hits in 842 CRISPR screens |
ChiTaRSi | PICK1, human |
EvolutionaryTracei | Q9NRD5 |
GeneWikii | PICK1 |
GenomeRNAii | 9463 |
Pharosi | Q9NRD5, Tbio |
PROi | PR:Q9NRD5 |
RNActi | Q9NRD5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000100151, Expressed in adenohypophysis and 172 other tissues |
ExpressionAtlasi | Q9NRD5, baseline and differential |
Genevisiblei | Q9NRD5, HS |
Family and domain databases
CDDi | cd07659, BAR_PICK1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR010504, AH_dom IPR030798, Arfaptin_fam IPR001478, PDZ IPR036034, PDZ_sf IPR037959, PICK1_BAR |
PANTHERi | PTHR12141, PTHR12141, 1 hit |
Pfami | View protein in Pfam PF06456, Arfaptin, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM01015, Arfaptin, 1 hit SM00228, PDZ, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50870, AH, 1 hit PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PICK1_HUMAN | |
Accessioni | Q9NRD5Primary (citable) accession number: Q9NRD5 Secondary accession number(s): B3KS52, O95906 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | August 2, 2002 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM