SPHK2

Functionⁱ

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.1 Publication

Catalytic activityⁱ

ATP + a sphingoid base = ADP + a sphingoid base 1-phosphate.1 Publication

Cofactorⁱ

Mg²⁺By similarity

Activity regulationⁱ

Inhibited by sulfatide.1 Publication

Kineticsⁱ

K_M=
3.4 µM for sphingosine
1 Publication
Manual assertion based on experiment inⁱ
- Ref.1
  "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform."
  Liu H., Sugiura M., Nava V.E., Edsall L.C., Kono K., Poulton S., Milstien S., Kohama T., Spiegel S.
  J. Biol. Chem. 275:19513-19520(2000) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.

pH dependenceⁱ

Optimum pH is 7.5.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	247	Proton donor/acceptorBy similarity	1
Binding siteⁱ	252	ATPPROSITE-ProRule annotation	1
Binding siteⁱ	344	SubstrateBy similarity	1
Binding siteⁱ	351	ATPPROSITE-ProRule annotation	1
Binding siteⁱ	357	ATPPROSITE-ProRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	188 – 190	ATPPROSITE-ProRule annotation	3
Nucleotide bindingⁱ	220 – 224	ATPPROSITE-ProRule annotation	5
Nucleotide bindingⁱ	277 – 279	ATPPROSITE-ProRule annotation	3
Nucleotide bindingⁱ	622 – 624	ATPPROSITE-ProRule annotation	3

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
D-erythro-sphingosine kinase activity Source: Reactome
NAD+ kinase activity Source: InterPro
Ras GTPase binding
Source: UniProtKB
Non-traceable author statementⁱ
- 12391145
sphinganine kinase activity Source: Reactome
sphingosine-1-phosphate receptor activity
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23106337

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

blood vessel development Source: Ensembl
brain development Source: Ensembl
cell proliferation
Source: UniProtKB
Non-traceable author statementⁱ
- 10751414
female pregnancy Source: Ensembl
negative regulation of apoptotic process
Source: UniProtKB
Non-traceable author statementⁱ
- 10751414
positive regulation of cell proliferation Source: Ensembl
sphinganine-1-phosphate biosynthetic process
Source: UniProtKB
Non-traceable author statementⁱ
- 10751414
sphingolipid biosynthetic process Source: Reactome
sphingosine biosynthetic process
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 23106337

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAⁱ	2.7.1.91 2681
Reactomeⁱ	R-HSA-1660661 Sphingolipid de novo biosynthesis
SIGNORⁱ	Q9NRA0

Chemistry databases

SwissLipidsⁱ	SLP:000000112

SwissLipidsⁱ

SLP:000000112

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Sphingosine kinase 2 (EC:2.7.1.911 Publication) Short name: SK 2 Short name: SPK 2
Gene namesⁱ	Name:SPHK2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 19

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000063176.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:18859 SPHK2
MIMⁱ	607092 gene
neXtProtⁱ	NX_Q9NRA0

Keywords - Cellular componentⁱ

Cytoplasm, Lysosome, Membrane

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	56848
Open Targets More...OpenTargetsⁱ	ENSG00000063176
PharmGKBⁱ	PA38719

Chemistry databases

ChEMBLⁱ	CHEMBL3023
IUPHAR/BPS Guide to PHARMACOLOGY More...GuidetoPHARMACOLOGYⁱ	2205

Polymorphism and mutation databases

BioMutaⁱ	SPHK2
DMDMⁱ	22001996

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000181358	1 – 654	Sphingosine kinase 2Add BLAST		654

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	387	PhosphoserineCombined sources	1
Modified residueⁱ	393	PhosphoserineBy similarity	1
Modified residueⁱ	399	PhosphoserineCombined sources	1
Modified residueⁱ	477	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q9NRA0
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NRA0
PaxDbⁱ	Q9NRA0
PeptideAtlas More...PeptideAtlasⁱ	Q9NRA0
PRIDEⁱ	Q9NRA0
ProteomicsDBⁱ	82315 82316 [Q9NRA0-2] 82317 [Q9NRA0-3]

PTM databases

iPTMnetⁱ	Q9NRA0
PhosphoSitePlusⁱ	Q9NRA0

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000063176 Expressed in 160 organ(s), highest expression level in substantia nigra
ExpressionAtlasⁱ	Q9NRA0 baseline and differential
Genevisibleⁱ	Q9NRA0 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA065508

HPAⁱ

HPA065508

Interactionⁱ

GO - Molecular functionⁱ

Ras GTPase binding
Source: UniProtKB
Non-traceable author statementⁱ
- 12391145

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	121208, 3 interactors
IntActⁱ	Q9NRA0, 5 interactors
STRINGⁱ	9606.ENSP00000245222

Chemistry databases

BindingDBⁱ

Q9NRA0

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9NRA0
SMRⁱ	Q9NRA0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	178 – 325	DAGKcPROSITE-ProRule annotationAdd BLAST		148

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	1 – 175	Required for binding to sulfatide and phosphoinositides and for membrane localizationAdd BLAST		175
Regionⁱ	245 – 248	Substrate bindingBy similarity		4

Phylogenomic databases

eggNOGⁱ	KOG1116 Eukaryota COG1597 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00690000101761
HOGENOMⁱ	HOG000111460
HOVERGENⁱ	HBG054796
InParanoidⁱ	Q9NRA0
KEGG Orthology (KO) More...KOⁱ	K04718
PhylomeDBⁱ	Q9NRA0
TreeFamⁱ	TF354296

Family and domain databases

Gene3Dⁱ	3.40.50.10330, 1 hit
InterProⁱ	View protein in InterPro IPR017438 ATP-NAD_kinase_N IPR001206 Diacylglycerol_kinase_cat_dom IPR016064 NAD/diacylglycerol_kinase_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00781 DAGK_cat, 1 hit
SMARTⁱ	View protein in SMART SM00046 DAGKc, 1 hit
SUPFAMⁱ	SSF111331 SSF111331, 1 hit
PROSITEⁱ	View protein in PROSITE PS50146 DAGK, 1 hit

Sequences (5+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 5 isoformsⁱ produced by alternative splicing. Align Add to basket

Note: Experimental confirmation may be lacking for some isoforms.

This entry has 5 described isoforms and 7 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9NRA0-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MNGHLEAEEQ QDQRPDQELT GSWGHGPRST LVRAKAMAPP PPPLAASTPL 
        60         70         80         90        100
LHGEFGSYPA RGPRFALTLT SQALHIQRLR PKPEARPRGG LVPLAEVSGC 
       110        120        130        140        150
CTLRSRSPSD SAAYFCIYTY PRGRRGARRR ATRTFRADGA ATYEENRAEA 
       160        170        180        190        200
QRWATALTCL LRGLPLPGDG EITPDLLPRP PRLLLLVNPF GGRGLAWQWC 
       210        220        230        240        250
KNHVLPMISE AGLSFNLIQT ERQNHARELV QGLSLSEWDG IVTVSGDGLL 
       260        270        280        290        300
HEVLNGLLDR PDWEEAVKMP VGILPCGSGN ALAGAVNQHG GFEPALGLDL 
       310        320        330        340        350
LLNCSLLLCR GGGHPLDLLS VTLASGSRCF SFLSVAWGFV SDVDIQSERF 
       360        370        380        390        400
RALGSARFTL GTVLGLATLH TYRGRLSYLP ATVEPASPTP AHSLPRAKSE 
       410        420        430        440        450
LTLTPDPAPP MAHSPLHRSV SDLPLPLPQP ALASPGSPEP LPILSLNGGG 
       460        470        480        490        500
PELAGDWGGA GDAPLSPDPL LSSPPGSPKA ALHSPVSEGA PVIPPSSGLP 
       510        520        530        540        550
LPTPDARVGA STCGPPDHLL PPLGTPLPPD WVTLEGDFVL MLAISPSHLG 
       560        570        580        590        600
ADLVAAPHAR FDDGLVHLCW VRSGISRAAL LRLFLAMERG SHFSLGCPQL 
       610        620        630        640        650
GYAAARAFRL EPLTPRGVLT VDGEQVEYGP LQAQMHPGIG TLLTGPPGCP 

GREP

Length:654

Mass (Da):69,217

Last modified:July 26, 2002 - v2

Checksum:ⁱF73FFCEC930DA50F

GO

Isoform 2 (identifier: Q9NRA0-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-36: Missing.

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        10         20         30         40         50
MAPPPPPLAA STPLLHGEFG SYPARGPRFA LTLTSQALHI QRLRPKPEAR 
        60         70         80         90        100
PRGGLVPLAE VSGCCTLRSR SPSDSAAYFC IYTYPRGRRG ARRRATRTFR 
       110        120        130        140        150
ADGAATYEEN RAEAQRWATA LTCLLRGLPL PGDGEITPDL LPRPPRLLLL 
       160        170        180        190        200
VNPFGGRGLA WQWCKNHVLP MISEAGLSFN LIQTERQNHA RELVQGLSLS 
       210        220        230        240        250
EWDGIVTVSG DGLLHEVLNG LLDRPDWEEA VKMPVGILPC GSGNALAGAV 
       260        270        280        290        300
NQHGGFEPAL GLDLLLNCSL LLCRGGGHPL DLLSVTLASG SRCFSFLSVA 
       310        320        330        340        350
WGFVSDVDIQ SERFRALGSA RFTLGTVLGL ATLHTYRGRL SYLPATVEPA 
       360        370        380        390        400
SPTPAHSLPR AKSELTLTPD PAPPMAHSPL HRSVSDLPLP LPQPALASPG 
       410        420        430        440        450
SPEPLPILSL NGGGPELAGD WGGAGDAPLS PDPLLSSPPG SPKAALHSPV 
       460        470        480        490        500
SEGAPVIPPS SGLPLPTPDA RVGASTCGPP DHLLPPLGTP LPPDWVTLEG 
       510        520        530        540        550
DFVLMLAISP SHLGADLVAA PHARFDDGLV HLCWVRSGIS RAALLRLFLA 
       560        570        580        590        600
MERGSHFSLG CPQLGYAAAR AFRLEPLTPR GVLTVDGEQV EYGPLQAQMH 
       610 
PGIGTLLTGP PGCPGREP

Show »

Length:618

Mass (Da):65,205

Checksum:ⁱ1A5B06BCB6F93F1F

GO

Isoform 3 (identifier: Q9NRA0-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-36: Missing.
292-390: FEPALGLDLL...ATVEPASPTP → PREDSDSSTS...NVFICKKKKK

« Hide

        10         20         30         40         50
MAPPPPPLAA STPLLHGEFG SYPARGPRFA LTLTSQALHI QRLRPKPEAR 
        60         70         80         90        100
PRGGLVPLAE VSGCCTLRSR SPSDSAAYFC IYTYPRGRRG ARRRATRTFR 
       110        120        130        140        150
ADGAATYEEN RAEAQRWATA LTCLLRGLPL PGDGEITPDL LPRPPRLLLL 
       160        170        180        190        200
VNPFGGRGLA WQWCKNHVLP MISEAGLSFN LIQTERQNHA RELVQGLSLS 
       210        220        230        240        250
EWDGIVTVSG DGLLHEVLNG LLDRPDWEEA VKMPVGILPC GSGNALAGAV 
       260        270        280        290        300
NQHGGPREDS DSSTSSSACP LWTTARSCPR AAASMPGSCP LLPQQLALGF 
       310        320        330        340        350
SRFIQDRVNG GGGRIGSLTC RGHTQRTLPA PAREGGGSLF LKNINVFICK 
       360        370        380        390        400
KKKKAHSLPR AKSELTLTPD PAPPMAHSPL HRSVSDLPLP LPQPALASPG 
       410        420        430        440        450
SPEPLPILSL NGGGPELAGD WGGAGDAPLS PDPLLSSPPG SPKAALHSPV 
       460        470        480        490        500
SEGAPVIPPS SGLPLPTPDA RVGASTCGPP DHLLPPLGTP LPPDWVTLEG 
       510        520        530        540        550
DFVLMLAISP SHLGADLVAA PHARFDDGLV HLCWVRSGIS RAALLRLFLA 
       560        570        580        590        600
MERGSHFSLG CPQLGYAAAR AFRLEPLTPR GVLTVDGEQV EYGPLQAQMH 
       610 
PGIGTLLTGP PGCPGREP

Show »

Length:618

Mass (Da):65,170

Checksum:ⁱD934F61C0A69EDAE

GO

Isoform 4 (identifier: Q9NRA0-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
13-71: Missing.

« Hide

        10         20         30         40         50
MNGHLEAEEQ QDQALHIQRL RPKPEARPRG GLVPLAEVSG CCTLRSRSPS 
        60         70         80         90        100
DSAAYFCIYT YPRGRRGARR RATRTFRADG AATYEENRAE AQRWATALTC 
       110        120        130        140        150
LLRGLPLPGD GEITPDLLPR PPRLLLLVNP FGGRGLAWQW CKNHVLPMIS 
       160        170        180        190        200
EAGLSFNLIQ TERQNHAREL VQGLSLSEWD GIVTVSGDGL LHEVLNGLLD 
       210        220        230        240        250
RPDWEEAVKM PVGILPCGSG NALAGAVNQH GGFEPALGLD LLLNCSLLLC 
       260        270        280        290        300
RGGGHPLDLL SVTLASGSRC FSFLSVAWGF VSDVDIQSER FRALGSARFT 
       310        320        330        340        350
LGTVLGLATL HTYRGRLSYL PATVEPASPT PAHSLPRAKS ELTLTPDPAP 
       360        370        380        390        400
PMAHSPLHRS VSDLPLPLPQ PALASPGSPE PLPILSLNGG GPELAGDWGG 
       410        420        430        440        450
AGDAPLSPDP LLSSPPGSPK AALHSPVSEG APVIPPSSGL PLPTPDARVG 
       460        470        480        490        500
ASTCGPPDHL LPPLGTPLPP DWVTLEGDFV LMLAISPSHL GADLVAAPHA 
       510        520        530        540        550
RFDDGLVHLC WVRSGISRAA LLRLFLAMER GSHFSLGCPQ LGYAAARAFR 
       560        570        580        590 
LEPLTPRGVL TVDGEQVEYG PLQAQMHPGI GTLLTGPPGC PGREP

Note: No experimental confirmation available.

Show »

Length:595

Mass (Da):62,986

Checksum:ⁱC24AC949EEEA059B

GO

Isoform 5 (identifier: Q9NRA0-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-12: MNGHLEAEEQQD → MIGCLHARVS...NSHPFSPPFP
640-654: GTLLTGPPGCPGREP → ARGRTQTPALPAAPALYGRQPGAAHGLQPVCQGAALFFHNSWLWGSQDLFRIVLTEAVGG

« Hide

        10         20         30         40         50
MIGCLHARVS GPLWDAGLCP ASSRSAHTCL SLSVSDAPVS PATAPHCLLL 
        60         70         80         90        100
STAPAPPCPC HGVLNSHPFS PPFPQRPDQE LTGSWGHGPR STLVRAKAMA 
       110        120        130        140        150
PPPPPLAAST PLLHGEFGSY PARGPRFALT LTSQALHIQR LRPKPEARPR 
       160        170        180        190        200
GGLVPLAEVS GCCTLRSRSP SDSAAYFCIY TYPRGRRGAR RRATRTFRAD 
       210        220        230        240        250
GAATYEENRA EAQRWATALT CLLRGLPLPG DGEITPDLLP RPPRLLLLVN 
       260        270        280        290        300
PFGGRGLAWQ WCKNHVLPMI SEAGLSFNLI QTERQNHARE LVQGLSLSEW 
       310        320        330        340        350
DGIVTVSGDG LLHEVLNGLL DRPDWEEAVK MPVGILPCGS GNALAGAVNQ 
       360        370        380        390        400
HGGFEPALGL DLLLNCSLLL CRGGGHPLDL LSVTLASGSR CFSFLSVAWG 
       410        420        430        440        450
FVSDVDIQSE RFRALGSARF TLGTVLGLAT LHTYRGRLSY LPATVEPASP 
       460        470        480        490        500
TPAHSLPRAK SELTLTPDPA PPMAHSPLHR SVSDLPLPLP QPALASPGSP 
       510        520        530        540        550
EPLPILSLNG GGPELAGDWG GAGDAPLSPD PLLSSPPGSP KAALHSPVSE 
       560        570        580        590        600
GAPVIPPSSG LPLPTPDARV GASTCGPPDH LLPPLGTPLP PDWVTLEGDF 
       610        620        630        640        650
VLMLAISPSH LGADLVAAPH ARFDDGLVHL CWVRSGISRA ALLRLFLAME 
       660        670        680        690        700
RGSHFSLGCP QLGYAAARAF RLEPLTPRGV LTVDGEQVEY GPLQAQMHPG 
       710        720        730        740        750
IARGRTQTPA LPAAPALYGR QPGAAHGLQP VCQGAALFFH NSWLWGSQDL 
       760 
FRIVLTEAVG G

Show »

Length:761

Mass (Da):80,205

Checksum:ⁱA52BF3441102A3D3

GO

Computationally mapped potential isoform sequencesⁱ

There are 7 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

M0R344	M0R344_HUMAN	Sphingosine kinase 2 Sphingosine kinase 2	SPHK2	663	Annotation score:
M0R0E9	M0R0E9_HUMAN	Sphingosine kinase 2 Sphingosine kinase 2	SPHK2	255	Annotation score:
E9PCV4	E9PCV4_HUMAN	Sphingosine kinase 2, isoform CRA_e Sphingosine kinase 2, isoform CRA_e	SPHK2 hCG_15274	448	Annotation score:
A0A075B7A1	A0A075B7A1_HUMAN	Sphingosine kinase 2 Sphingosine kinase 2	SPHK2	349	Annotation score:
M0QZP3	M0QZP3_HUMAN	Sphingosine kinase 2 Sphingosine kinase 2	SPHK2	72	Annotation score:
M0QXX5	M0QXX5_HUMAN	Sphingosine kinase 2 Sphingosine kinase 2	SPHK2	133	Annotation score:
E7ET12	E7ET12_HUMAN	Sphingosine kinase 2 Sphingosine kinase 2	SPHK2	49	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	49	P → S in CAB66636 (PubMed:11230166).Curated		1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_060112	652	R → Q. Corresponds to variant dbSNP:rs11881285Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_006217	1 – 36	Missing in isoform 2 and isoform 3. 4 PublicationsAdd BLAST	36
Alternative sequenceⁱVSP_047721	1 – 12	MNGHL…EEQQD → MIGCLHARVSGPLWDAGLCP ASSRSAHTCLSLSVSDAPVS PATAPHCLLLSTAPAPPCPC HGVLNSHPFSPPFP in isoform 5. 1 PublicationAdd BLAST	12
Alternative sequenceⁱVSP_046910	13 – 71	Missing in isoform 4. 1 PublicationAdd BLAST	59
Alternative sequenceⁱVSP_006218	292 – 390	FEPAL…ASPTP → PREDSDSSTSSSACPLWTTA RSCPRAAASMPGSCPLLPQQ LALGFSRFIQDRVNGGGGRI GSLTCRGHTQRTLPAPAREG GGSLFLKNINVFICKKKKK in isoform 3. 1 PublicationAdd BLAST	99
Alternative sequenceⁱVSP_047722	640 – 654	GTLLT…PGREP → ARGRTQTPALPAAPALYGRQ PGAAHGLQPVCQGAALFFHN SWLWGSQDLFRIVLTEAVGG in isoform 5. 1 PublicationAdd BLAST	15

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF245447 mRNA Translation: AAF74124.1 EF107108 mRNA Translation: ABK81123.1 AL136701 mRNA Translation: CAB66636.1 AK000599 mRNA Translation: BAA91280.1 AK300541 mRNA Translation: BAG62249.1 AC022154 Genomic DNA No translation available. BC006161 mRNA Translation: AAH06161.1 BC010671 mRNA Translation: AAH10671.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS12727.1 [Q9NRA0-1] CCDS59404.1 [Q9NRA0-4] CCDS59405.1 [Q9NRA0-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_001191087.1, NM_001204158.2 [Q9NRA0-4] NP_001191088.1, NM_001204159.2 [Q9NRA0-1] NP_001191089.1, NM_001204160.2 [Q9NRA0-2] NP_064511.2, NM_020126.4 [Q9NRA0-1] XP_006723355.1, XM_006723292.1 [Q9NRA0-2] XP_011525435.1, XM_011527133.1 [Q9NRA0-1] XP_011525436.1, XM_011527134.1 [Q9NRA0-2]
UniGeneⁱ	Hs.528006

Genome annotation databases

Ensemblⁱ	ENST00000245222; ENSP00000245222; ENSG00000063176 [Q9NRA0-1] ENST00000598088; ENSP00000469158; ENSG00000063176 [Q9NRA0-1] ENST00000599748; ENSP00000471205; ENSG00000063176 [Q9NRA0-2] ENST00000600537; ENSP00000470092; ENSG00000063176 [Q9NRA0-4]
GeneIDⁱ	56848
KEGGⁱ	hsa:56848
UCSC genome browser More...UCSCⁱ	uc002pjr.4 human [Q9NRA0-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NRA0	Sphingosine kinase 2, isoform CRA_c		HUMAN		654	UniRef100_Q9NRA0
Q9NRA0-2	Sphingosine kinase 2, isoform CRA_e		HUMAN		448	UniRef100_E9PCV4
cDNA FLJ90120 fis, clone HEMBA1007013		HUMAN		244
UPI0003EAEEA8		HUMAN		716
Q9NRA0-3	Sphingosine kinase 2		HUMAN		349	UniRef100_A0A075B7A1
Q9NRA0-5	Sphingosine kinase 2		HUMAN		663	UniRef100_M0R344
SPHK2 isoform 3		PONAB		255
Sphingosine kinase 2		HUMAN		255
Sphingosine kinase 2 (Fragment)		HUMAN		72
SPHK2 isoform 9 (Fragment)		PONAB		72
+2

Protein	Similar proteins		Species	Score	Length	Source
Q9NRA0	Sphingosine kinase 2, isoform CRA_c		HUMAN		654	UniRef90_Q9NRA0
Sphingosine kinase 2		CANLF		674
Uncharacterized protein		MUSPF		673
sphingosine kinase 2 isoform X1		Enhydra lutris kenyoni		669
sphingosine kinase 2 isoform X1		BALAS		665
+137
Q9NRA0-2	Uncharacterized protein		CHLSB		653	UniRef90_A0A0D9S2X3
Uncharacterized protein		RHIRO		665
Sphingosine kinase 2		HUMAN		663
SPHK2 isoform 3		PONAB		255
Uncharacterized protein		MANLE		663
+66
Q9NRA0-3	Sphingosine kinase 2		HUMAN		349	UniRef90_A0A075B7A1
Q9NRA0-4	sphingosine kinase 2 isoform X3		ODORO		596	UniRef90_Q9NRA0-4
SPHK2 isoform 4		PANTR		595
SPHK2 isoform 4		PONAB		595
UPI000C9EB57C		HORSE		704
UPI0003ACCEFF		EQUPR		448
+7
Q9NRA0-5	Uncharacterized protein		CHLSB		653	UniRef90_A0A0D9S2X3
Uncharacterized protein		RHIRO		665
Sphingosine kinase 2		HUMAN		663
SPHK2 isoform 3		PONAB		255
Uncharacterized protein		MANLE		663
+66

Protein	Similar proteins		Species	Score	Length	Source
Q9NRA0	Sphingosine kinase 2		MOUSE		617	UniRef50_Q9NRA0
Sphingosine kinase 2, isoform CRA_c		HUMAN		654
Sphingosine kinase 2		CANLF		674
Uncharacterized protein		MUSPF		673
sphingosine kinase 2 isoform X1		Enhydra lutris kenyoni		669
+338

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF245447 mRNA Translation: AAF74124.1 EF107108 mRNA Translation: ABK81123.1 AL136701 mRNA Translation: CAB66636.1 AK000599 mRNA Translation: BAA91280.1 AK300541 mRNA Translation: BAG62249.1 AC022154 Genomic DNA No translation available. BC006161 mRNA Translation: AAH06161.1 BC010671 mRNA Translation: AAH10671.1
CCDSⁱ	CCDS12727.1 [Q9NRA0-1] CCDS59404.1 [Q9NRA0-4] CCDS59405.1 [Q9NRA0-2]
RefSeqⁱ	NP_001191087.1, NM_001204158.2 [Q9NRA0-4] NP_001191088.1, NM_001204159.2 [Q9NRA0-1] NP_001191089.1, NM_001204160.2 [Q9NRA0-2] NP_064511.2, NM_020126.4 [Q9NRA0-1] XP_006723355.1, XM_006723292.1 [Q9NRA0-2] XP_011525435.1, XM_011527133.1 [Q9NRA0-1] XP_011525436.1, XM_011527134.1 [Q9NRA0-2]
UniGeneⁱ	Hs.528006

3D structure databases

ProteinModelPortalⁱ	Q9NRA0
SMRⁱ	Q9NRA0
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	121208, 3 interactors
IntActⁱ	Q9NRA0, 5 interactors
STRINGⁱ	9606.ENSP00000245222

Chemistry databases

BindingDBⁱ	Q9NRA0
ChEMBLⁱ	CHEMBL3023
GuidetoPHARMACOLOGYⁱ	2205
SwissLipidsⁱ	SLP:000000112

PTM databases

iPTMnetⁱ	Q9NRA0
PhosphoSitePlusⁱ	Q9NRA0

Polymorphism and mutation databases

BioMutaⁱ	SPHK2
DMDMⁱ	22001996

Proteomic databases

EPDⁱ	Q9NRA0
MaxQBⁱ	Q9NRA0
PaxDbⁱ	Q9NRA0
PeptideAtlasⁱ	Q9NRA0
PRIDEⁱ	Q9NRA0
ProteomicsDBⁱ	82315 82316 [Q9NRA0-2] 82317 [Q9NRA0-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	56848
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000245222; ENSP00000245222; ENSG00000063176 [Q9NRA0-1] ENST00000598088; ENSP00000469158; ENSG00000063176 [Q9NRA0-1] ENST00000599748; ENSP00000471205; ENSG00000063176 [Q9NRA0-2] ENST00000600537; ENSP00000470092; ENSG00000063176 [Q9NRA0-4]
GeneIDⁱ	56848
KEGGⁱ	hsa:56848
UCSCⁱ	uc002pjr.4 human [Q9NRA0-1]

Organism-specific databases

CTDⁱ	56848
DisGeNETⁱ	56848
EuPathDBⁱ	HostDB:ENSG00000063176.15
GeneCardsⁱ	SPHK2
HGNCⁱ	HGNC:18859 SPHK2
HPAⁱ	HPA065508
MIMⁱ	607092 gene
neXtProtⁱ	NX_Q9NRA0
OpenTargetsⁱ	ENSG00000063176
PharmGKBⁱ	PA38719
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG1116 Eukaryota COG1597 LUCA
GeneTreeⁱ	ENSGT00690000101761
HOGENOMⁱ	HOG000111460
HOVERGENⁱ	HBG054796
InParanoidⁱ	Q9NRA0
KOⁱ	K04718
PhylomeDBⁱ	Q9NRA0
TreeFamⁱ	TF354296

Enzyme and pathway databases

BRENDAⁱ	2.7.1.91 2681
Reactomeⁱ	R-HSA-1660661 Sphingolipid de novo biosynthesis
SIGNORⁱ	Q9NRA0

Miscellaneous databases

ChiTaRSⁱ	SPHK2 human
GeneWikiⁱ	SPHK2
GenomeRNAiⁱ	56848
Protein Ontology More...PROⁱ	PR:Q9NRA0
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000063176 Expressed in 160 organ(s), highest expression level in substantia nigra
ExpressionAtlasⁱ	Q9NRA0 baseline and differential
Genevisibleⁱ	Q9NRA0 HS

Family and domain databases

Gene3Dⁱ	3.40.50.10330, 1 hit
InterProⁱ	View protein in InterPro IPR017438 ATP-NAD_kinase_N IPR001206 Diacylglycerol_kinase_cat_dom IPR016064 NAD/diacylglycerol_kinase_sf
Pfamⁱ	View protein in Pfam PF00781 DAGK_cat, 1 hit
SMARTⁱ	View protein in SMART SM00046 DAGKc, 1 hit
SUPFAMⁱ	SSF111331 SSF111331, 1 hit
PROSITEⁱ	View protein in PROSITE PS50146 DAGK, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPHK2_HUMAN
Accessionⁱ	Q9NRA0Primary (citable) accession number: Q9NRA0 Secondary accession number(s): A0T4C8 , B4DU87, Q9BRN1, Q9H0Q2, Q9NWU7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 24, 2001
	Last sequence update:	July 26, 2002
	Last modified:	November 7, 2018
	This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM

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UniProtKB - Q9NRA0 (SPHK2_HUMAN)

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Sphingosine kinase 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

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<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

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Chemistry databases

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Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

Kineticsⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ