UniProtKB - Q9NR81 (ARHG3_HUMAN)
Protein
Rho guanine nucleotide exchange factor 3
Gene
ARHGEF3
Organism
Homo sapiens (Human)
Status
Functioni
Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases.1 Publication
GO - Molecular functioni
- Rho guanyl-nucleotide exchange factor activity Source: GO_Central
GO - Biological processi
- G protein-coupled receptor signaling pathway Source: Reactome
- positive regulation of apoptotic process Source: Reactome
- positive regulation of Rho protein signal transduction Source: GO_Central
- regulation of small GTPase mediated signal transduction Source: Reactome
- Rho protein signal transduction Source: ProtInc
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
PathwayCommonsi | Q9NR81 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-194840, Rho GTPase cycle R-HSA-416482, G alpha (12/13) signalling events |
SIGNORi | Q9NR81 |
Names & Taxonomyi
Protein namesi | Recommended name: Rho guanine nucleotide exchange factor 3Alternative name(s): Exchange factor found in platelets and leukemic and neuronal tissues Short name: XPLN |
Gene namesi | Name:ARHGEF3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000163947.11 |
HGNCi | HGNC:683, ARHGEF3 |
MIMi | 612115, gene |
neXtProti | NX_Q9NR81 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 50650 |
OpenTargetsi | ENSG00000163947 |
PharmGKBi | PA24973 |
Miscellaneous databases
Pharosi | Q9NR81, Tbio |
Polymorphism and mutation databases
BioMutai | ARHGEF3 |
DMDMi | 52782760 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080911 | 1 – 526 | Rho guanine nucleotide exchange factor 3Add BLAST | 526 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 47 | PhosphoserineCombined sources | 1 | |
Modified residuei | 70 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9NR81 |
jPOSTi | Q9NR81 |
MassIVEi | Q9NR81 |
MaxQBi | Q9NR81 |
PaxDbi | Q9NR81 |
PeptideAtlasi | Q9NR81 |
PRIDEi | Q9NR81 |
ProteomicsDBi | 82296 [Q9NR81-1] 82297 [Q9NR81-2] 82298 [Q9NR81-3] 82299 [Q9NR81-4] |
PTM databases
iPTMneti | Q9NR81 |
PhosphoSitePlusi | Q9NR81 |
Expressioni
Tissue specificityi
Widely expressed. Highest levels are found in adult brain and skeletal muscle. Lower levels are found in heart and kidney.2 Publications
Gene expression databases
Bgeei | ENSG00000163947, Expressed in metanephric glomerulus and 235 other tissues |
ExpressionAtlasi | Q9NR81, baseline and differential |
Genevisiblei | Q9NR81, HS |
Organism-specific databases
HPAi | ENSG00000163947, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with RHOA and RHOB.
Binary interactionsi
Hide detailsQ9NR81
GO - Molecular functioni
- Rho guanyl-nucleotide exchange factor activity Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 119115, 14 interactors |
IntActi | Q9NR81, 11 interactors |
MINTi | Q9NR81 |
STRINGi | 9606.ENSP00000341071 |
Miscellaneous databases
RNActi | Q9NR81, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 122 – 304 | DHPROSITE-ProRule annotationAdd BLAST | 183 | |
Domaini | 291 – 449 | PHPROSITE-ProRule annotationAdd BLAST | 159 |
Phylogenomic databases
eggNOGi | KOG4305, Eukaryota |
GeneTreei | ENSGT00940000158385 |
InParanoidi | Q9NR81 |
OMAi | PIPMKDL |
OrthoDBi | 849206at2759 |
PhylomeDBi | Q9NR81 |
TreeFami | TF328974 |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain |
Pfami | View protein in Pfam PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 9 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NR81-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVAKDYPFYL TVKRANCSLE LPPASGPAKD AEEPSNKRVK PLSRVTSLAN
60 70 80 90 100
LIPPVKATPL KRFSQTLQRS ISFRSESRPD ILAPRPWSRN AAPSSTKRRD
110 120 130 140 150
SKLWSETFDV CVNQMLTSKE IKRQEAIFEL SQGEEDLIED LKLAKKAYHD
160 170 180 190 200
PMLKLSIMTE QELNQIFGTL DSLIPLHEEL LSQLRDVRKP DGSTEHVGPI
210 220 230 240 250
LVGWLPCLSS YDSYCSNQVA AKALLDHKKQ DHRVQDFLQR CLESPFSRKL
260 270 280 290 300
DLWNFLDIPR SRLVKYPLLL REILRHTPND NPDQQHLEEA INIIQGIVAE
310 320 330 340 350
INTKTGESEC RYYKERLLYL EEGQKDSLID SSRVLCCHGE LKNNRGVKLH
360 370 380 390 400
VFLFQEVLVI TRAVTHNEQL CYQLYRQPIP VKDLLLEDLQ DGEVRLGGSL
410 420 430 440 450
RGAFSNNERI KNFFRVSFKN GSQSQTHSLQ ANDTFNKQQW LNCIRQAKET
460 470 480 490 500
VLCAAGQAGV LDSEGSFLNP TTGSRELQGE TKLEQMDQSD SESDCSMDTS
510 520
EVSLDCERME QTDSSCGNSR HGESNV
Computationally mapped potential isoform sequencesi
There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PG37 | E9PG37_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 532 | Annotation score: | ||
E7EU49 | E7EU49_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 497 | Annotation score: | ||
C9J586 | C9J586_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 416 | Annotation score: | ||
C9J609 | C9J609_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 184 | Annotation score: | ||
C9JNF2 | C9JNF2_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 196 | Annotation score: | ||
H7C4P0 | H7C4P0_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 236 | Annotation score: | ||
C9J5K8 | C9J5K8_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 100 | Annotation score: | ||
F8WC29 | F8WC29_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 50 | Annotation score: | ||
F8WDB7 | F8WDB7_HUMAN | Rho guanine nucleotide exchange fac... | ARHGEF3 | 70 | Annotation score: |
Sequence cautioni
The sequence AAH68513 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB14891 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD92898 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 410 | I → T in AAP97313 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021935 | 13 | K → R. Corresponds to variant dbSNP:rs3732507Ensembl. | 1 | |
Natural variantiVAR_021936 | 335 | L → V4 PublicationsCorresponds to variant dbSNP:rs3772219Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027204 | 1 – 202 | Missing in isoform 4. 1 PublicationAdd BLAST | 202 | |
Alternative sequenceiVSP_011612 | 1 – 32 | MVAKD…AKDAE → MDSSTAMNQCSCRGMEENKE RPKRQRQNNFPMFPSPKAWN FRGRKRKQSTQDEDAVSLCS LDIS in isoform 2. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_027205 | 1 – 32 | MVAKD…AKDAE → MIEVCHHNWLLWLCPSKFGI FMCCLASTTGQMELRRTR in isoform 3. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_027206 | 203 – 204 | GW → MK in isoform 4. 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF249744 mRNA Translation: AAF79954.1 AL136832 mRNA Translation: CAB66766.1 AF433662 mRNA Translation: AAP97313.1 AK024340 mRNA Translation: BAB14891.1 Different initiation. AK291412 mRNA Translation: BAF84101.1 AB209661 mRNA Translation: BAD92898.1 Different initiation. CH471055 Genomic DNA Translation: EAW65326.1 BC054345 mRNA Translation: AAH54345.2 BC068513 mRNA Translation: AAH68513.1 Different initiation. BC098122 mRNA Translation: AAH98122.2 BC098272 mRNA Translation: AAH98272.2 BC099715 mRNA Translation: AAH99715.1 BC104723 mRNA Translation: AAI04724.2 |
CCDSi | CCDS2878.1 [Q9NR81-1] CCDS46854.1 [Q9NR81-2] CCDS46855.1 [Q9NR81-3] |
RefSeqi | NP_001122087.1, NM_001128615.1 [Q9NR81-2] NP_001122088.1, NM_001128616.1 [Q9NR81-3] NP_001276627.1, NM_001289698.1 NP_062455.1, NM_019555.2 [Q9NR81-1] XP_005265243.1, XM_005265186.4 [Q9NR81-2] XP_005265244.1, XM_005265187.3 |
Genome annotation databases
Ensembli | ENST00000296315; ENSP00000296315; ENSG00000163947 [Q9NR81-1] ENST00000338458; ENSP00000341071; ENSG00000163947 [Q9NR81-2] ENST00000413728; ENSP00000410922; ENSG00000163947 [Q9NR81-3] |
GeneIDi | 50650 |
KEGGi | hsa:50650 |
UCSCi | uc003dif.3, human [Q9NR81-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF249744 mRNA Translation: AAF79954.1 AL136832 mRNA Translation: CAB66766.1 AF433662 mRNA Translation: AAP97313.1 AK024340 mRNA Translation: BAB14891.1 Different initiation. AK291412 mRNA Translation: BAF84101.1 AB209661 mRNA Translation: BAD92898.1 Different initiation. CH471055 Genomic DNA Translation: EAW65326.1 BC054345 mRNA Translation: AAH54345.2 BC068513 mRNA Translation: AAH68513.1 Different initiation. BC098122 mRNA Translation: AAH98122.2 BC098272 mRNA Translation: AAH98272.2 BC099715 mRNA Translation: AAH99715.1 BC104723 mRNA Translation: AAI04724.2 |
CCDSi | CCDS2878.1 [Q9NR81-1] CCDS46854.1 [Q9NR81-2] CCDS46855.1 [Q9NR81-3] |
RefSeqi | NP_001122087.1, NM_001128615.1 [Q9NR81-2] NP_001122088.1, NM_001128616.1 [Q9NR81-3] NP_001276627.1, NM_001289698.1 NP_062455.1, NM_019555.2 [Q9NR81-1] XP_005265243.1, XM_005265186.4 [Q9NR81-2] XP_005265244.1, XM_005265187.3 |
3D structure databases
SMRi | Q9NR81 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119115, 14 interactors |
IntActi | Q9NR81, 11 interactors |
MINTi | Q9NR81 |
STRINGi | 9606.ENSP00000341071 |
PTM databases
iPTMneti | Q9NR81 |
PhosphoSitePlusi | Q9NR81 |
Polymorphism and mutation databases
BioMutai | ARHGEF3 |
DMDMi | 52782760 |
Proteomic databases
EPDi | Q9NR81 |
jPOSTi | Q9NR81 |
MassIVEi | Q9NR81 |
MaxQBi | Q9NR81 |
PaxDbi | Q9NR81 |
PeptideAtlasi | Q9NR81 |
PRIDEi | Q9NR81 |
ProteomicsDBi | 82296 [Q9NR81-1] 82297 [Q9NR81-2] 82298 [Q9NR81-3] 82299 [Q9NR81-4] |
Protocols and materials databases
Antibodypediai | 31503, 173 antibodies |
DNASUi | 50650 |
Genome annotation databases
Ensembli | ENST00000296315; ENSP00000296315; ENSG00000163947 [Q9NR81-1] ENST00000338458; ENSP00000341071; ENSG00000163947 [Q9NR81-2] ENST00000413728; ENSP00000410922; ENSG00000163947 [Q9NR81-3] |
GeneIDi | 50650 |
KEGGi | hsa:50650 |
UCSCi | uc003dif.3, human [Q9NR81-1] |
Organism-specific databases
CTDi | 50650 |
DisGeNETi | 50650 |
EuPathDBi | HostDB:ENSG00000163947.11 |
GeneCardsi | ARHGEF3 |
HGNCi | HGNC:683, ARHGEF3 |
HPAi | ENSG00000163947, Low tissue specificity |
MIMi | 612115, gene |
neXtProti | NX_Q9NR81 |
OpenTargetsi | ENSG00000163947 |
PharmGKBi | PA24973 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4305, Eukaryota |
GeneTreei | ENSGT00940000158385 |
InParanoidi | Q9NR81 |
OMAi | PIPMKDL |
OrthoDBi | 849206at2759 |
PhylomeDBi | Q9NR81 |
TreeFami | TF328974 |
Enzyme and pathway databases
PathwayCommonsi | Q9NR81 |
Reactomei | R-HSA-193648, NRAGE signals death through JNK R-HSA-194840, Rho GTPase cycle R-HSA-416482, G alpha (12/13) signalling events |
SIGNORi | Q9NR81 |
Miscellaneous databases
BioGRID-ORCSi | 50650, 5 hits in 844 CRISPR screens |
ChiTaRSi | ARHGEF3, human |
GeneWikii | ARHGEF3 |
GenomeRNAii | 50650 |
Pharosi | Q9NR81, Tbio |
PROi | PR:Q9NR81 |
RNActi | Q9NR81, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163947, Expressed in metanephric glomerulus and 235 other tissues |
ExpressionAtlasi | Q9NR81, baseline and differential |
Genevisiblei | Q9NR81, HS |
Family and domain databases
CDDi | cd00160, RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR035899, DBL_dom_sf IPR000219, DH-domain IPR001331, GDS_CDC24_CS IPR011993, PH-like_dom_sf IPR001849, PH_domain |
Pfami | View protein in Pfam PF00621, RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00233, PH, 1 hit SM00325, RhoGEF, 1 hit |
SUPFAMi | SSF48065, SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS00741, DH_1, 1 hit PS50010, DH_2, 1 hit PS50003, PH_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARHG3_HUMAN | |
Accessioni | Q9NR81Primary (citable) accession number: Q9NR81 Secondary accession number(s): A8K5U7 Q9H7T4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 27, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations