UniProtKB - Q9NQU5 (PAK6_HUMAN)
Serine/threonine-protein kinase PAK 6
PAK6
Functioni
Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.
2 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 436 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 526 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 413 – 421 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cadherin binding Source: BHF-UCL
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- apoptotic process Source: UniProtKB
- cytoskeleton organization Source: UniProtKB
- intracellular signal transduction Source: GO_Central
- learning Source: Ensembl
- locomotory behavior Source: Ensembl
- memory Source: Ensembl
- neuron projection arborization Source: Ensembl
- neuron projection extension Source: Ensembl
- protein phosphorylation Source: GO_Central
- regulation of MAPK cascade Source: GO_Central
- regulation of transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q9NQU5 |
Reactomei | R-HSA-428540, Activation of RAC1 R-HSA-9013148, CDC42 GTPase cycle R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013405, RHOD GTPase cycle R-HSA-9013407, RHOH GTPase cycle R-HSA-9013424, RHOV GTPase cycle |
SignaLinki | Q9NQU5 |
SIGNORi | Q9NQU5 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein kinase PAK 6 (EC:2.7.11.1)Alternative name(s): PAK-5 p21-activated kinase 6 Short name: PAK-6 |
Gene namesi | Name:PAK6 Synonyms:PAK5 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16061, PAK6 |
MIMi | 608110, gene |
neXtProti | NX_Q9NQU5 |
VEuPathDBi | HostDB:ENSG00000137843 |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Nucleus
- fibrillar center Source: HPA
- nucleoplasm Source: HPA
Other locations
- cell junction Source: HPA
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 165 | S → A: Almost complete loss of PAK6 activation by MAP2K6/MAPKK6; when associated with F-566. 1 Publication | 1 | |
Mutagenesisi | 560 | S → A: Complete loss of PAK6 activation by MAP2K6/MAPKK6; when associated with A-165. 1 Publication | 1 | |
Mutagenesisi | 566 | Y → F: Complete loss of PAK6 activation by MAP2K6/MAPKK6; when associated with A-165. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 106821730 56924 |
OpenTargetsi | ENSG00000137843 |
PharmGKBi | PA32921 |
Miscellaneous databases
Pharosi | Q9NQU5, Tchem |
Chemistry databases
ChEMBLi | CHEMBL4311 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q9NQU5 |
GuidetoPHARMACOLOGYi | 2137 |
Genetic variation databases
BioMutai | PAK6 |
DMDMi | 23396789 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000086476 | 1 – 681 | Serine/threonine-protein kinase PAK 6Add BLAST | 681 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 560 | Phosphoserine; by autocatalysisBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9NQU5 |
jPOSTi | Q9NQU5 |
MassIVEi | Q9NQU5 |
MaxQBi | Q9NQU5 |
PaxDbi | Q9NQU5 |
PeptideAtlasi | Q9NQU5 |
PRIDEi | Q9NQU5 |
ProteomicsDBi | 34018 82189 [Q9NQU5-1] |
PTM databases
iPTMneti | Q9NQU5 |
PhosphoSitePlusi | Q9NQU5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000137843, Expressed in prefrontal cortex and 119 other tissues |
ExpressionAtlasi | Q9NQU5, baseline and differential |
Genevisiblei | Q9NQU5, HS |
Organism-specific databases
HPAi | ENSG00000137843, Tissue enhanced (esophagus, skin) |
Interactioni
Subunit structurei
Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity).
Interacts with the androgen receptor AR and the estrogen receptor ESR1.
Interacts with IQGAP1 and PPM1B.
By similarity4 PublicationsBinary interactionsi
Q9NQU5
GO - Molecular functioni
- cadherin binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 121251, 40 interactors |
IntActi | Q9NQU5, 22 interactors |
MINTi | Q9NQU5 |
STRINGi | 9606.ENSP00000406873 |
Chemistry databases
BindingDBi | Q9NQU5 |
Miscellaneous databases
RNActi | Q9NQU5, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9NQU5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NQU5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 12 – 25 | CRIBPROSITE-ProRule annotationAdd BLAST | 14 | |
Domaini | 407 – 658 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 252 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 30 | DisorderedSequence analysisAdd BLAST | 30 | |
Regioni | 26 – 406 | LinkerAdd BLAST | 381 | |
Regioni | 149 – 169 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 200 – 256 | DisorderedSequence analysisAdd BLAST | 57 | |
Regioni | 268 – 355 | DisorderedSequence analysisAdd BLAST | 88 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 275 – 289 | Pro residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 290 – 355 | Polar residuesSequence analysisAdd BLAST | 66 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0578, Eukaryota |
GeneTreei | ENSGT00940000156528 |
HOGENOMi | CLU_000288_26_6_1 |
InParanoidi | Q9NQU5 |
OMAi | HRQMPWP |
PhylomeDBi | Q9NQU5 |
TreeFami | TF105352 |
Family and domain databases
CDDi | cd01093, CRIB_PAK_like, 1 hit |
Gene3Di | 3.90.810.10, 1 hit |
InterProi | View protein in InterPro IPR000095, CRIB_dom IPR036936, CRIB_dom_sf IPR011009, Kinase-like_dom_sf IPR035066, PAK6 IPR033923, PAK_BD IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
PANTHERi | PTHR45832:SF3, PTHR45832:SF3, 1 hit |
Pfami | View protein in Pfam PF00786, PBD, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00285, PBD, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50108, CRIB, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 10 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFRKKKKKRP EISAPQNFQH RVHTSFDPKE GKFVGLPPQW QNILDTLRRP
60 70 80 90 100
KPVVDPSRIT RVQLQPMKTV VRGSAMPVDG YISGLLNDIQ KLSVISSNTL
110 120 130 140 150
RGRSPTSRRR AQSLGLLGDE HWATDPDMYL QSPQSERTDP HGLYLSCNGG
160 170 180 190 200
TPAGHKQMPW PEPQSPRVLP NGLAAKAQSL GPAEFQGASQ RCLQLGACLQ
210 220 230 240 250
SSPPGASPPT GTNRHGMKAA KHGSEEARPQ SCLVGSATGR PGGEGSPSPK
260 270 280 290 300
TRESSLKRRL FRSMFLSTAA TAPPSSSKPG PPPQSKPNSS FRPPQKDNPP
310 320 330 340 350
SLVAKAQSLP SDQPVGTFSP LTTSDTSSPQ KSLRTAPATG QLPGRSSPAG
360 370 380 390 400
SPRTWHAQIS TSNLYLPQDP TVAKGALAGE DTGVVTHEQF KAALRMVVDQ
410 420 430 440 450
GDPRLLLDSY VKIGEGSTGI VCLAREKHSG RQVAVKMMDL RKQQRRELLF
460 470 480 490 500
NEVVIMRDYQ HFNVVEMYKS YLVGEELWVL MEFLQGGALT DIVSQVRLNE
510 520 530 540 550
EQIATVCEAV LQALAYLHAQ GVIHRDIKSD SILLTLDGRV KLSDFGFCAQ
560 570 580 590 600
ISKDVPKRKS LVGTPYWMAP EVISRSLYAT EVDIWSLGIM VIEMVDGEPP
610 620 630 640 650
YFSDSPVQAM KRLRDSPPPK LKNSHKVSPV LRDFLERMLV RDPQERATAQ
660 670 680
ELLDHPFLLQ TGLPECLVPL IQLYRKQTST C
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0YL16 | H0YL16_HUMAN | Non-specific serine/threonine prote... | PAK6 | 284 | Annotation score: | ||
H0YKB6 | H0YKB6_HUMAN | Non-specific serine/threonine prote... | PAK6 | 51 | Annotation score: | ||
H0YLZ9 | H0YLZ9_HUMAN | Non-specific serine/threonine prote... | PAK6 | 122 | Annotation score: | ||
H0YM99 | H0YM99_HUMAN | Non-specific serine/threonine prote... | PAK6 | 104 | Annotation score: | ||
H0YK74 | H0YK74_HUMAN | Non-specific serine/threonine prote... | PAK6 | 109 | Annotation score: | ||
H0YK91 | H0YK91_HUMAN | Non-specific serine/threonine prote... | PAK6 | 112 | Annotation score: | ||
H0YMS4 | H0YMS4_HUMAN | Non-specific serine/threonine prote... | PAK6 | 123 | Annotation score: | ||
H0YK38 | H0YK38_HUMAN | Non-specific serine/threonine prote... | PAK6 | 68 | Annotation score: | ||
H0YL47 | H0YL47_HUMAN | Non-specific serine/threonine prote... | PAK6 | 65 | Annotation score: | ||
H0YL02 | H0YL02_HUMAN | Serine/threonine-protein kinase PAK... | PAK6 | 14 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 60 | T → A in BAG54772 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 381 | D → G in BAG54772 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035631 | 3 | R → H in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs201346085Ensembl. | 1 | |
Natural variantiVAR_019993 | 76 | M → V1 PublicationCorresponds to variant dbSNP:rs2412504Ensembl. | 1 | |
Natural variantiVAR_051655 | 103 | R → C. Corresponds to variant dbSNP:rs36081263Ensembl. | 1 | |
Natural variantiVAR_051656 | 151 | T → I. Corresponds to variant dbSNP:rs35593179Ensembl. | 1 | |
Natural variantiVAR_040972 | 184 | E → K1 PublicationCorresponds to variant dbSNP:rs56349744Ensembl. | 1 | |
Natural variantiVAR_040973 | 205 | G → E1 PublicationCorresponds to variant dbSNP:rs55920845Ensembl. | 1 | |
Natural variantiVAR_040974 | 208 | P → T1 PublicationCorresponds to variant dbSNP:rs35501648EnsemblClinVar. | 1 | |
Natural variantiVAR_040975 | 210 | T → M1 PublicationCorresponds to variant dbSNP:rs34869667Ensembl. | 1 | |
Natural variantiVAR_019994 | 215 | H → R1 PublicationCorresponds to variant dbSNP:rs3743135Ensembl. | 1 | |
Natural variantiVAR_019995 | 337 | P → L1 PublicationCorresponds to variant dbSNP:rs3743137Ensembl. | 1 | |
Natural variantiVAR_040976 | 376 | A → V1 PublicationCorresponds to variant dbSNP:rs55806501Ensembl. | 1 | |
Natural variantiVAR_051657 | 475 | E → K. Corresponds to variant dbSNP:rs34445577Ensembl. | 1 | |
Natural variantiVAR_040977 | 514 | L → R in a lung small cell carcinoma sample; somatic mutation. 1 Publication | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056733 | 583 – 627 | Missing in isoform 2. 1 PublicationAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF276893 mRNA Translation: AAF82800.1 AJ236915 mRNA Translation: CAC18720.1 AK131522 mRNA Translation: BAG54772.1 AK290227 mRNA Translation: BAF82916.1 AK315813 mRNA Translation: BAF98704.1 AK315817 mRNA Translation: BAF98708.1 AC020658 Genomic DNA No translation available. AC025429 Genomic DNA No translation available. CH471125 Genomic DNA Translation: EAW92395.1 CH471125 Genomic DNA Translation: EAW92401.1 BC035596 mRNA Translation: AAH35596.1 |
CCDSi | CCDS10054.1 [Q9NQU5-1] CCDS61590.1 [Q9NQU5-2] |
RefSeqi | NP_001122100.1, NM_001128628.2 [Q9NQU5-1] NP_001122101.1, NM_001128629.2 [Q9NQU5-1] NP_001263646.1, NM_001276717.1 [Q9NQU5-1] NP_001263647.1, NM_001276718.1 [Q9NQU5-2] NP_064553.1, NM_020168.5 [Q9NQU5-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF276893 mRNA Translation: AAF82800.1 AJ236915 mRNA Translation: CAC18720.1 AK131522 mRNA Translation: BAG54772.1 AK290227 mRNA Translation: BAF82916.1 AK315813 mRNA Translation: BAF98704.1 AK315817 mRNA Translation: BAF98708.1 AC020658 Genomic DNA No translation available. AC025429 Genomic DNA No translation available. CH471125 Genomic DNA Translation: EAW92395.1 CH471125 Genomic DNA Translation: EAW92401.1 BC035596 mRNA Translation: AAH35596.1 |
CCDSi | CCDS10054.1 [Q9NQU5-1] CCDS61590.1 [Q9NQU5-2] |
RefSeqi | NP_001122100.1, NM_001128628.2 [Q9NQU5-1] NP_001122101.1, NM_001128629.2 [Q9NQU5-1] NP_001263646.1, NM_001276717.1 [Q9NQU5-1] NP_001263647.1, NM_001276718.1 [Q9NQU5-2] NP_064553.1, NM_020168.5 [Q9NQU5-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2C30 | X-ray | 1.60 | A | 383-681 | [»] | |
2ODB | X-ray | 2.40 | B | 11-45 | [»] | |
4KS7 | X-ray | 1.40 | A | 385-674 | [»] | |
4KS8 | X-ray | 1.95 | A | 385-674 | [»] | |
6QDR | X-ray | 1.61 | B | 94-104 | [»] | |
6QDS | X-ray | 1.72 | B | 94-104 | [»] | |
SMRi | Q9NQU5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121251, 40 interactors |
IntActi | Q9NQU5, 22 interactors |
MINTi | Q9NQU5 |
STRINGi | 9606.ENSP00000406873 |
Chemistry databases
BindingDBi | Q9NQU5 |
ChEMBLi | CHEMBL4311 |
DrugBanki | DB12010, Fostamatinib |
DrugCentrali | Q9NQU5 |
GuidetoPHARMACOLOGYi | 2137 |
PTM databases
iPTMneti | Q9NQU5 |
PhosphoSitePlusi | Q9NQU5 |
Genetic variation databases
BioMutai | PAK6 |
DMDMi | 23396789 |
Proteomic databases
EPDi | Q9NQU5 |
jPOSTi | Q9NQU5 |
MassIVEi | Q9NQU5 |
MaxQBi | Q9NQU5 |
PaxDbi | Q9NQU5 |
PeptideAtlasi | Q9NQU5 |
PRIDEi | Q9NQU5 |
ProteomicsDBi | 34018 82189 [Q9NQU5-1] |
Protocols and materials databases
Antibodypediai | 10054, 542 antibodies from 39 providers |
DNASUi | 56924 |
Genome annotation databases
Organism-specific databases
CTDi | 106821730 56924 |
DisGeNETi | 106821730 56924 |
GeneCardsi | PAK6 |
HGNCi | HGNC:16061, PAK6 |
HPAi | ENSG00000137843, Tissue enhanced (esophagus, skin) |
MIMi | 608110, gene |
neXtProti | NX_Q9NQU5 |
OpenTargetsi | ENSG00000137843 |
PharmGKBi | PA32921 |
VEuPathDBi | HostDB:ENSG00000137843 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0578, Eukaryota |
GeneTreei | ENSGT00940000156528 |
HOGENOMi | CLU_000288_26_6_1 |
InParanoidi | Q9NQU5 |
OMAi | HRQMPWP |
PhylomeDBi | Q9NQU5 |
TreeFami | TF105352 |
Enzyme and pathway databases
PathwayCommonsi | Q9NQU5 |
Reactomei | R-HSA-428540, Activation of RAC1 R-HSA-9013148, CDC42 GTPase cycle R-HSA-9013149, RAC1 GTPase cycle R-HSA-9013405, RHOD GTPase cycle R-HSA-9013407, RHOH GTPase cycle R-HSA-9013424, RHOV GTPase cycle |
SignaLinki | Q9NQU5 |
SIGNORi | Q9NQU5 |
Miscellaneous databases
BioGRID-ORCSi | 106821730, 0 hits in 11 CRISPR screens 56924, 8 hits in 1068 CRISPR screens |
ChiTaRSi | PAK6, human |
EvolutionaryTracei | Q9NQU5 |
GeneWikii | PAK6 |
Pharosi | Q9NQU5, Tchem |
PROi | PR:Q9NQU5 |
RNActi | Q9NQU5, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000137843, Expressed in prefrontal cortex and 119 other tissues |
ExpressionAtlasi | Q9NQU5, baseline and differential |
Genevisiblei | Q9NQU5, HS |
Family and domain databases
CDDi | cd01093, CRIB_PAK_like, 1 hit |
Gene3Di | 3.90.810.10, 1 hit |
InterProi | View protein in InterPro IPR000095, CRIB_dom IPR036936, CRIB_dom_sf IPR011009, Kinase-like_dom_sf IPR035066, PAK6 IPR033923, PAK_BD IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
PANTHERi | PTHR45832:SF3, PTHR45832:SF3, 1 hit |
Pfami | View protein in Pfam PF00786, PBD, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00285, PBD, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50108, CRIB, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PAK6_HUMAN | |
Accessioni | Q9NQU5Primary (citable) accession number: Q9NQU5 Secondary accession number(s): A8K2G2, B3KYB0, G5E9R2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2002 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 195 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families