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Protein

Omega-amidase NIT2

Gene

NIT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.2 Publications

Catalytic activityi

A monoamide of a dicarboxylate + H2O = a dicarboxylate + NH3.

Kineticsi

In solution, alpha-ketoglutaramate is in equilibrium with a cyclic form (2-hydroxy-5-oxoproline), and at pH 8.0 or above, the rate of ring opening is no longer limiting for the omega-amidase reaction.
  1. KM=0.009 mM for alpha-ketoglutaramate (open-chain form)1 Publication
  1. Vmax=5.9 µmol/min/mg enzyme with alpha-ketoglutaramate as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei43Proton acceptorPROSITE-ProRule annotation1
Active sitei112Proton donorPROSITE-ProRule annotation1
Active sitei153NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • omega-amidase activity Source: BHF-UCL

GO - Biological processi

  • asparagine metabolic process Source: BHF-UCL
  • glutamine metabolic process Source: BHF-UCL
  • neutrophil degranulation Source: Reactome
  • oxaloacetate metabolic process Source: BHF-UCL

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Omega-amidase NIT2 (EC:3.5.1.3)
Alternative name(s):
Nitrilase homolog 2
Gene namesi
Name:NIT2
ORF Names:CUA002
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000114021.11
HGNCiHGNC:29878 NIT2
neXtProtiNX_Q9NQR4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000114021
PharmGKBiPA134882857

Polymorphism and mutation databases

BioMutaiNIT2
DMDMi74725271

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003202521 – 276Omega-amidase NIT2Add BLAST276

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineCombined sources1
Modified residuei68N6-acetyllysine; alternateBy similarity1
Modified residuei68N6-succinyllysine; alternateBy similarity1
Modified residuei123N6-succinyllysineBy similarity1
Modified residuei130N6-succinyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NQR4
PaxDbiQ9NQR4
PeptideAtlasiQ9NQR4
PRIDEiQ9NQR4
ProteomicsDBi82173

2D gel databases

REPRODUCTION-2DPAGEiIPI00549467
UCD-2DPAGEiQ9NQR4

PTM databases

iPTMnetiQ9NQR4
PhosphoSitePlusiQ9NQR4

Expressioni

Tissue specificityi

Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.2 Publications

Gene expression databases

BgeeiENSG00000114021
CleanExiHS_NIT2
ExpressionAtlasiQ9NQR4 baseline and differential
GenevisibleiQ9NQR4 HS

Organism-specific databases

HPAiHPA036999

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi121278, 26 interactors
IntActiQ9NQR4, 2 interactors
MINTiQ9NQR4
STRINGi9606.ENSP00000377696

Structurei

3D structure databases

ProteinModelPortaliQ9NQR4
SMRiQ9NQR4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 248CN hydrolasePROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0806 Eukaryota
COG0388 LUCA
GeneTreeiENSGT00550000074838
HOGENOMiHOG000222700
HOVERGENiHBG105126
InParanoidiQ9NQR4
KOiK13566
OMAiTGKDHWQ
OrthoDBiEOG091G0IKZ
PhylomeDBiQ9NQR4
TreeFamiTF300747

Family and domain databases

Gene3Di3.60.110.10, 1 hit
InterProiView protein in InterPro
IPR003010 C-N_Hydrolase
IPR036526 C-N_Hydrolase_sf
PfamiView protein in Pfam
PF00795 CN_hydrolase, 1 hit
SUPFAMiSSF56317 SSF56317, 1 hit
PROSITEiView protein in PROSITE
PS50263 CN_HYDROLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NQR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG
60 70 80 90 100
AKYFPEYAEK IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF
110 120 130 140 150
GPDGTLLAKY RKIHLFDIDV PGKITFQESK TLSPGDSFST FDTPYCRVGL
160 170 180 190 200
GICYDMRFAE LAQIYAQRGC QLLVYPGAFN LTTGPAHWEL LQRSRAVDNQ
210 220 230 240 250
VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA IVYSDIDLKK
260 270
LAEIRQQIPV FRQKRSDLYA VEMKKP
Length:276
Mass (Da):30,608
Last modified:October 1, 2000 - v1
Checksum:i32FA797601A419C6
GO

Mass spectrometryi

Molecular mass is 30585 Da from positions 1 - 276. Determined by MALDI. 1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_039180231V → A1 PublicationCorresponds to variant dbSNP:rs17851799Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF284574 mRNA Translation: AAF87103.1
AF260334 mRNA Translation: AAG44665.1
AK313704 mRNA Translation: BAG36450.1
CH471052 Genomic DNA Translation: EAW79824.1
CH471052 Genomic DNA Translation: EAW79825.1
BC020620 mRNA Translation: AAH20620.1
BC107890 mRNA Translation: AAI07891.1
CCDSiCCDS33806.1
RefSeqiNP_064587.1, NM_020202.4
UniGeneiHs.439152

Genome annotation databases

EnsembliENST00000394140; ENSP00000377696; ENSG00000114021
GeneIDi56954
KEGGihsa:56954

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiNIT2_HUMAN
AccessioniPrimary (citable) accession number: Q9NQR4
Secondary accession number(s): B2R9A3, D3DN47, Q8WUF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: July 18, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

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