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Protein

Ras-related GTP-binding protein D

Gene

RRAGD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 77GTP9
Nucleotide bindingi117 – 121GTP5
Nucleotide bindingi179 – 182GTP4

GO - Molecular functioni

  • GDP binding Source: CAFA
  • GTPase activity Source: CAFA
  • GTPase binding Source: CAFA
  • GTP binding Source: CAFA
  • protein heterodimerization activity Source: CAFA

GO - Biological processi

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-165159 mTOR signalling
R-HSA-166208 mTORC1-mediated signalling
R-HSA-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-HSA-5628897 TP53 Regulates Metabolic Genes
R-HSA-8943724 Regulation of PTEN gene transcription
SIGNORiQ9NQL2

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related GTP-binding protein D
Short name:
Rag D
Short name:
RagD
Gene namesi
Name:RRAGDImported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000025039.14
HGNCiHGNC:19903 RRAGD
MIMi608268 gene
neXtProtiNX_Q9NQL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Lysosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76S → L: Increased RPTOR-binding. 1 Publication1
Mutagenesisi121Q → L: Decreased RPTOR-binding. 1 Publication1

Organism-specific databases

DisGeNETi58528
OpenTargetsiENSG00000025039
PharmGKBiPA134957148

Polymorphism and mutation databases

BioMutaiRRAGD
DMDMi74752904

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002399531 – 400Ras-related GTP-binding protein DAdd BLAST400

Proteomic databases

EPDiQ9NQL2
MaxQBiQ9NQL2
PaxDbiQ9NQL2
PeptideAtlasiQ9NQL2
PRIDEiQ9NQL2
ProteomicsDBi82161
82162 [Q9NQL2-2]

PTM databases

iPTMnetiQ9NQL2
PhosphoSitePlusiQ9NQL2

Expressioni

Gene expression databases

BgeeiENSG00000025039 Expressed in 240 organ(s), highest expression level in saliva-secreting gland
CleanExiHS_RRAGD
GenevisibleiQ9NQL2 HS

Organism-specific databases

HPAiHPA068664

Interactioni

Subunit structurei

Forms a heterodimer with RRAGA in a sequence-independent manner and RRAGB. Heterodimerization stabilizes RRAG proteins. In complex with RRAGB, interacts with RPTOR; this interaction is particularly efficient with GTP-loaded RRAGB and GDP-loaded RRAGC. Interacts with NOL8. Interacts with SH3BP4; the interaction with this negative regulator is most probably direct, preferentially occurs with the inactive GDP-bound form of RRAGD and is negatively regulated by amino acids (PubMed:11073942, PubMed:14660641, PubMed:18497260, PubMed:22575674, Ref. 14). The Rag heterodimer interacts with SLC38A9; the probable amino acid sensor (PubMed:25561175, PubMed:25567906). Interacts with SESN1, SESN2 AND SESN3 (PubMed:25259925).8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121848, 14 interactors
IntActiQ9NQL2, 10 interactors
STRINGi9606.ENSP00000358423

Structurei

Secondary structure

1400
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9NQL2
SMRiQ9NQL2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NQL2

Family & Domainsi

Sequence similaritiesi

Belongs to the GTR/RAG GTP-binding protein family.Curated

Phylogenomic databases

eggNOGiKOG3887 Eukaryota
ENOG410XQS9 LUCA
GeneTreeiENSGT00550000074708
HOGENOMiHOG000203695
HOVERGENiHBG059482
InParanoidiQ9NQL2
KOiK16186
OMAiDIQTYEV
OrthoDBiEOG091G0AN4
PhylomeDBiQ9NQL2
TreeFamiTF300659

Family and domain databases

CDDicd11385 RagC_like, 1 hit
InterProiView protein in InterPro
IPR006762 Gtr1_RagA
IPR027417 P-loop_NTPase
IPR039400 RagC/D
PANTHERiPTHR11259 PTHR11259, 1 hit
PfamiView protein in Pfam
PF04670 Gtr1_RagA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 11 Publication (identifier: Q9NQL2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQVLGKPQP QDEDDAEEEE EEDELVGLAD YGDGPDSSDA DPDSGTEEGV
60 70 80 90 100
LDFSDPFSTE VKPRILLMGL RRSGKSSIQK VVFHKMSPNE TLFLESTNKI
110 120 130 140 150
CREDVSNSSF VNFQIWDFPG QIDFFDPTFD YEMIFRGTGA LIFVIDSQDD
160 170 180 190 200
YMEALARLHL TVTRAYKVNT DINFEVFIHK VDGLSDDHKI ETQRDIHQRA
210 220 230 240 250
NDDLADAGLE KIHLSFYLTS IYDHSIFEAF SKVVQKLIPQ LPTLENLLNI
260 270 280 290 300
FISNSGIEKA FLFDVVSKIY IATDSTPVDM QTYELCCDMI DVVIDISCIY
310 320 330 340 350
GLKEDGAGTP YDKESTAIIK LNNTTVLYLK EVTKFLALVC FVREESFERK
360 370 380 390 400
GLIDYNFHCF RKAIHEVFEV RMKVVKSRKV QNRLQKKKRA TPNGTPRVLL
Length:400
Mass (Da):45,588
Last modified:October 1, 2000 - v1
Checksum:i96FF0854B11AA1BC
GO
Isoform 21 Publication (identifier: Q9NQL2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-151: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:249
Mass (Da):28,611
Checksum:i4A6435D188BE8283
GO

Sequence cautioni

The sequence AAH03088 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0520781 – 151Missing in isoform 2. 1 PublicationAdd BLAST151

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF272036 mRNA Translation: AAG32663.1
AK289799 mRNA Translation: BAF82488.1
AL138717 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48554.1
AL137502 mRNA Translation: CAB70775.2
BC003088 mRNA Translation: AAH03088.1 Different initiation.
CCDSiCCDS5022.1 [Q9NQL2-1]
PIRiT46254
RefSeqiNP_067067.1, NM_021244.4 [Q9NQL2-1]
UniGeneiHs.31712

Genome annotation databases

EnsembliENST00000359203; ENSP00000352131; ENSG00000025039 [Q9NQL2-2]
ENST00000369415; ENSP00000358423; ENSG00000025039 [Q9NQL2-1]
GeneIDi58528
KEGGihsa:58528
UCSCiuc003pnd.5 human [Q9NQL2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF272036 mRNA Translation: AAG32663.1
AK289799 mRNA Translation: BAF82488.1
AL138717 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48554.1
AL137502 mRNA Translation: CAB70775.2
BC003088 mRNA Translation: AAH03088.1 Different initiation.
CCDSiCCDS5022.1 [Q9NQL2-1]
PIRiT46254
RefSeqiNP_067067.1, NM_021244.4 [Q9NQL2-1]
UniGeneiHs.31712

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q3FX-ray2.10A/B60-239[»]
ProteinModelPortaliQ9NQL2
SMRiQ9NQL2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121848, 14 interactors
IntActiQ9NQL2, 10 interactors
STRINGi9606.ENSP00000358423

PTM databases

iPTMnetiQ9NQL2
PhosphoSitePlusiQ9NQL2

Polymorphism and mutation databases

BioMutaiRRAGD
DMDMi74752904

Proteomic databases

EPDiQ9NQL2
MaxQBiQ9NQL2
PaxDbiQ9NQL2
PeptideAtlasiQ9NQL2
PRIDEiQ9NQL2
ProteomicsDBi82161
82162 [Q9NQL2-2]

Protocols and materials databases

DNASUi58528
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000359203; ENSP00000352131; ENSG00000025039 [Q9NQL2-2]
ENST00000369415; ENSP00000358423; ENSG00000025039 [Q9NQL2-1]
GeneIDi58528
KEGGihsa:58528
UCSCiuc003pnd.5 human [Q9NQL2-1]

Organism-specific databases

CTDi58528
DisGeNETi58528
EuPathDBiHostDB:ENSG00000025039.14
GeneCardsiRRAGD
HGNCiHGNC:19903 RRAGD
HPAiHPA068664
MIMi608268 gene
neXtProtiNX_Q9NQL2
OpenTargetsiENSG00000025039
PharmGKBiPA134957148
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3887 Eukaryota
ENOG410XQS9 LUCA
GeneTreeiENSGT00550000074708
HOGENOMiHOG000203695
HOVERGENiHBG059482
InParanoidiQ9NQL2
KOiK16186
OMAiDIQTYEV
OrthoDBiEOG091G0AN4
PhylomeDBiQ9NQL2
TreeFamiTF300659

Enzyme and pathway databases

ReactomeiR-HSA-1632852 Macroautophagy
R-HSA-165159 mTOR signalling
R-HSA-166208 mTORC1-mediated signalling
R-HSA-380972 Energy dependent regulation of mTOR by LKB1-AMPK
R-HSA-5628897 TP53 Regulates Metabolic Genes
R-HSA-8943724 Regulation of PTEN gene transcription
SIGNORiQ9NQL2

Miscellaneous databases

ChiTaRSiRRAGD human
EvolutionaryTraceiQ9NQL2
GenomeRNAii58528
PROiPR:Q9NQL2
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000025039 Expressed in 240 organ(s), highest expression level in saliva-secreting gland
CleanExiHS_RRAGD
GenevisibleiQ9NQL2 HS

Family and domain databases

CDDicd11385 RagC_like, 1 hit
InterProiView protein in InterPro
IPR006762 Gtr1_RagA
IPR027417 P-loop_NTPase
IPR039400 RagC/D
PANTHERiPTHR11259 PTHR11259, 1 hit
PfamiView protein in Pfam
PF04670 Gtr1_RagA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRRAGD_HUMAN
AccessioniPrimary (citable) accession number: Q9NQL2
Secondary accession number(s): A8K184, Q7L8F9, Q9NPG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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