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Protein

Anoctamin-2

Gene

ANO2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-activated chloride channel (CaCC) which may play a role in olfactory signal transduction. Odorant molecules bind to odor-sensing receptors (OSRs), leading to an increase in calcium entry that activates CaCC current which amplifies the depolarization of the OSR cells, ANO2 seems to be the underlying chloride channel involved in this process. May mediate light perception amplification in retina.4 Publications

Miscellaneous

The term 'anoctamin' was coined because these channels are anion selective and have eight (OCT) transmembrane segments. There is some dissatisfaction in the field with the Ano nomenclature because it is not certain that all the members of this family are anion channels or have the 8-transmembrane topology.

Activity regulationi

Channel activity is repressed by chloride inhibitors; strongly by niflumic acid (NFA), partially by flufenamic acid (FFA), and only slightly by meclofenamic acid (MFA), 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), 4-acetamido-4'-isothiocyanato-stilben-2,2'-disulfonate (SITS), and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChloride channel, Ion channel
Biological processIon transport, Transport
LigandCalcium, Chloride

Enzyme and pathway databases

ReactomeiR-HSA-2672351 Stimuli-sensing channels

Protein family/group databases

TCDBi1.A.17.1.3 the calcium-dependent chloride channel (ca-clc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Anoctamin-2
Alternative name(s):
Transmembrane protein 16B
Gene namesi
Name:ANO2
Synonyms:C12orf3, TMEM16B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000047617.14
HGNCiHGNC:1183 ANO2
MIMi610109 gene
neXtProtiNX_Q9NQ90

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 365CytoplasmicSequence analysisAdd BLAST365
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 434ExtracellularSequence analysisAdd BLAST48
Transmembranei435 – 455HelicalSequence analysisAdd BLAST21
Topological domaini456 – 538CytoplasmicSequence analysisAdd BLAST83
Transmembranei539 – 559HelicalSequence analysisAdd BLAST21
Topological domaini560 – 582ExtracellularSequence analysisAdd BLAST23
Transmembranei583 – 603HelicalSequence analysisAdd BLAST21
Topological domaini604 – 623CytoplasmicSequence analysisAdd BLAST20
Transmembranei624 – 644HelicalSequence analysisAdd BLAST21
Topological domaini645 – 748ExtracellularSequence analysisAdd BLAST104
Transmembranei749 – 769HelicalSequence analysisAdd BLAST21
Topological domaini770 – 801CytoplasmicSequence analysisAdd BLAST32
Transmembranei802 – 822HelicalSequence analysisAdd BLAST21
Topological domaini823 – 907ExtracellularSequence analysisAdd BLAST85
Transmembranei908 – 928HelicalSequence analysisAdd BLAST21
Topological domaini929 – 1003CytoplasmicSequence analysisAdd BLAST75

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi57101
OpenTargetsiENSG00000047617
PharmGKBiPA25504

Polymorphism and mutation databases

DMDMi262527528

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000725641 – 1003Anoctamin-2Add BLAST1003

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi422N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi841N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi849N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi856N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9NQ90
PeptideAtlasiQ9NQ90
PRIDEiQ9NQ90
ProteomicsDBi82108
82109 [Q9NQ90-2]

PTM databases

iPTMnetiQ9NQ90
PhosphoSitePlusiQ9NQ90

Expressioni

Tissue specificityi

Retina, especially in the photoreceptor synaptic terminals.1 Publication

Gene expression databases

BgeeiENSG00000047617 Expressed in 99 organ(s), highest expression level in testis
CleanExiHS_ANO2
ExpressionAtlasiQ9NQ90 baseline and differential
GenevisibleiQ9NQ90 HS

Organism-specific databases

HPAiHPA036276
HPA057499

Interactioni

Subunit structurei

Component of a presynaptic protein complex recruited to specialized plasma membrane domains of photoreceptors. Interacts with DLG4 by its C-terminal region (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9606.ENSP00000314048

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1001 – 1003DLG4 binding (PDZ)3

Sequence similaritiesi

Belongs to the anoctamin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2514 Eukaryota
ENOG410XS4S LUCA
GeneTreeiENSGT00760000119015
HOGENOMiHOG000006509
HOVERGENiHBG069519
InParanoidiQ9NQ90
KOiK19497
PhylomeDBiQ9NQ90
TreeFamiTF314265

Family and domain databases

InterProiView protein in InterPro
IPR032394 Anoct_dimer
IPR007632 Anoctamin
IPR031288 Anoctamin-2
PANTHERiPTHR12308 PTHR12308, 1 hit
PTHR12308:SF20 PTHR12308:SF20, 1 hit
PfamiView protein in Pfam
PF16178 Anoct_dimer, 1 hit
PF04547 Anoctamin, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NQ90-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATPGPRDIP LLPGSPRRLS PQAGSRGGQG PKHGQQCLKM PGPRAPGLQG
60 70 80 90 100
GSNRDPGQPC GGESTRSSSV INNYLDANEP VSLEARLSRM HFHDSQRKVD
110 120 130 140 150
YVLAYHYRKR GVHLAQGFPG HSLAIVSNGE TGKEPHAGGP GDIELGPLDA
160 170 180 190 200
LEEERKEQRE EFEHNLMEAG LELEKDLENK SQGSIFVRIH APWQVLAREA
210 220 230 240 250
EFLKIKVPTK KEMYEIKAGG SIAKKFSAAL QKLSSHLQPR VPEHSNNKMK
260 270 280 290 300
NLSYPFSREK MYLYNIQEKD TFFDNATRSR IVHEILKRTA CSRANNTMGI
310 320 330 340 350
NSLIANNIYE AAYPLHDGEY DSPEDDMNDR KLLYQEWARY GVFYKFQPID
360 370 380 390 400
LIRKYFGEKI GLYFAWLGLY TSFLIPSSVI GVIVFLYGCA TIEEDIPSRE
410 420 430 440 450
MCDQQNAFTM CPLCDKSCDY WNLSSACGTA QASHLFDNPA TVFFSIFMAL
460 470 480 490 500
WATMFLENWK RLQMRLGYFW DLTGIEEEEE RAQEHSRPEY ETKVREKMLK
510 520 530 540 550
ESNQSAVQKL ETNTTECGDE DDEDKLTWKD RFPGYLMNFA SILFMIALTF
560 570 580 590 600
SIVFGVIVYR ITTAAALSLN KATRSNVRVT VTATAVIINL VVILILDEIY
610 620 630 640 650
GAVAKWLTKI EVPKTEQTFE ERLILKAFLL KFVNAYSPIF YVAFFKGRFV
660 670 680 690 700
GRPGSYVYVF DGYRMEECAP GGCLMELCIQ LSIIMLGKQL IQNNIFEIGV
710 720 730 740 750
PKLKKLFRKL KDETEAGETD SAHSKHPEQW DLDYSLEPYT GLTPEYMEMI
760 770 780 790 800
IQFGFVTLFV ASFPLAPVFA LLNNVIEVRL DAKKFVTELR RPDAVRTKDI
810 820 830 840 850
GIWFDILSGI GKFSVISNAF VIAITSDFIP RLVYQYSYSH NGTLHGFVNH
860 870 880 890 900
TLSFFNVSQL KEGTQPENSQ FDQEVQFCRF KDYREPPWAP NPYEFSKQYW
910 920 930 940 950
FILSARLAFV IIFQNLVMFL SVLVDWMIPD IPTDISDQIK KEKSLLVDFF
960 970 980 990 1000
LKEEHEKLKL MDEPALRSPG GGDRSRSRAA SSAPSGQSQL GSMMSSGSQH

TNV
Note: Splice site between exons 4 and 5 is non-canonical.
Length:1,003
Mass (Da):113,969
Last modified:November 3, 2009 - v2
Checksum:i32939EADC8AFE5C8
GO
Isoform 2 (identifier: Q9NQ90-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.
     5-7: GPR → MPE

Note: Splice site between exons 4 and 5 is non-canonical.
Show »
Length:999
Mass (Da):113,616
Checksum:iB9B4F56161AE1B00
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F5GXT3F5GXT3_HUMAN
Anoctamin
ANO2
1,003Annotation score:
F5H806F5H806_HUMAN
Anoctamin
ANO2
203Annotation score:
Q69YW4Q69YW4_HUMAN
Anoctamin-2
ANO2 DKFZp434P102
86Annotation score:

Sequence cautioni

The sequence BAB14773 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021932112V → A. Corresponds to variant dbSNP:rs3741903Ensembl.1
Natural variantiVAR_057286147P → S. Corresponds to variant dbSNP:rs3741901Ensembl.1
Natural variantiVAR_061853401M → I. Corresponds to variant dbSNP:rs17788563Ensembl.1
Natural variantiVAR_020331505S → A. Corresponds to variant dbSNP:rs1860961Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0404931 – 4Missing in isoform 2. 1 Publication4
Alternative sequenceiVSP_0404945 – 7GPR → MPE in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272204 mRNA Translation: CAC01125.1
FJ384095 mRNA Translation: ACL36050.1
AC006431 Genomic DNA No translation available.
AC006560 Genomic DNA No translation available.
AC137627 Genomic DNA No translation available.
AK024010 mRNA Translation: BAB14773.1 Different initiation.
CCDSiCCDS44807.2 [Q9NQ90-2]
RefSeqiNP_001265525.1, NM_001278596.1 [Q9NQ90-1]
NP_001265526.1, NM_001278597.1 [Q9NQ90-2]
UniGeneiHs.148970

Genome annotation databases

EnsembliENST00000327087; ENSP00000314048; ENSG00000047617 [Q9NQ90-2]
ENST00000356134; ENSP00000348453; ENSG00000047617 [Q9NQ90-2]
GeneIDi57101
KEGGihsa:57101
UCSCiuc058kbk.1 human [Q9NQ90-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ272204 mRNA Translation: CAC01125.1
FJ384095 mRNA Translation: ACL36050.1
AC006431 Genomic DNA No translation available.
AC006560 Genomic DNA No translation available.
AC137627 Genomic DNA No translation available.
AK024010 mRNA Translation: BAB14773.1 Different initiation.
CCDSiCCDS44807.2 [Q9NQ90-2]
RefSeqiNP_001265525.1, NM_001278596.1 [Q9NQ90-1]
NP_001265526.1, NM_001278597.1 [Q9NQ90-2]
UniGeneiHs.148970

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000314048

Protein family/group databases

TCDBi1.A.17.1.3 the calcium-dependent chloride channel (ca-clc) family

PTM databases

iPTMnetiQ9NQ90
PhosphoSitePlusiQ9NQ90

Polymorphism and mutation databases

DMDMi262527528

Proteomic databases

PaxDbiQ9NQ90
PeptideAtlasiQ9NQ90
PRIDEiQ9NQ90
ProteomicsDBi82108
82109 [Q9NQ90-2]

Protocols and materials databases

DNASUi57101
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000327087; ENSP00000314048; ENSG00000047617 [Q9NQ90-2]
ENST00000356134; ENSP00000348453; ENSG00000047617 [Q9NQ90-2]
GeneIDi57101
KEGGihsa:57101
UCSCiuc058kbk.1 human [Q9NQ90-1]

Organism-specific databases

CTDi57101
DisGeNETi57101
EuPathDBiHostDB:ENSG00000047617.14
GeneCardsiANO2
HGNCiHGNC:1183 ANO2
HPAiHPA036276
HPA057499
MIMi610109 gene
neXtProtiNX_Q9NQ90
OpenTargetsiENSG00000047617
PharmGKBiPA25504
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2514 Eukaryota
ENOG410XS4S LUCA
GeneTreeiENSGT00760000119015
HOGENOMiHOG000006509
HOVERGENiHBG069519
InParanoidiQ9NQ90
KOiK19497
PhylomeDBiQ9NQ90
TreeFamiTF314265

Enzyme and pathway databases

ReactomeiR-HSA-2672351 Stimuli-sensing channels

Miscellaneous databases

ChiTaRSiANO2 human
GenomeRNAii57101
PROiPR:Q9NQ90
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000047617 Expressed in 99 organ(s), highest expression level in testis
CleanExiHS_ANO2
ExpressionAtlasiQ9NQ90 baseline and differential
GenevisibleiQ9NQ90 HS

Family and domain databases

InterProiView protein in InterPro
IPR032394 Anoct_dimer
IPR007632 Anoctamin
IPR031288 Anoctamin-2
PANTHERiPTHR12308 PTHR12308, 1 hit
PTHR12308:SF20 PTHR12308:SF20, 1 hit
PfamiView protein in Pfam
PF16178 Anoct_dimer, 1 hit
PF04547 Anoctamin, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANO2_HUMAN
AccessioniPrimary (citable) accession number: Q9NQ90
Secondary accession number(s): C4N787, Q9H847
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 3, 2009
Last modified: October 10, 2018
This is version 131 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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