UniProtKB - Q9NQ86 (TRI36_HUMAN)
Protein
E3 ubiquitin-protein ligase TRIM36
Gene
TRIM36
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Involved in chromosome segregation and cell cycle regulation (PubMed:28087737). May play a role in the acrosome reaction and fertilization.By similarity1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.By similarity EC:2.3.2.27
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 33 – 84 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 52 | |
Zinc fingeri | 154 – 192 | B box-type 1PROSITE-ProRule annotationAdd BLAST | 39 | |
Zinc fingeri | 207 – 249 | B box-type 2PROSITE-ProRule annotationAdd BLAST | 43 |
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
- ubiquitin-protein transferase activity Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- acrosome reaction Source: Ensembl
- mitotic cytokinesis Source: UniProtKB
- protein polyubiquitination Source: Reactome
- regulation of cell cycle Source: Ensembl
- regulation of microtubule cytoskeleton organization Source: UniProtKB
- spindle organization Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9NQ86 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase TRIM36 (EC:2.3.2.27By similarity)Alternative name(s): RING finger protein 98 RING-type E3 ubiquitin transferase TRIM36Curated Tripartite motif-containing protein 36 Zinc-binding protein Rbcc728 |
Gene namesi | Name:TRIM36 Synonyms:RBCC728, RNF98 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:16280, TRIM36 |
MIMi | 609317, gene |
neXtProti | NX_Q9NQ86 |
VEuPathDBi | HostDB:ENSG00000152503.9 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- cytoskeleton By similarity
Other locations
- acrosome By similarity
Note: Found in the acrosomal region of elongated spermatids and mature sperm.By similarity
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Cytosol
- cytosol Source: Reactome
Other locations
- acrosomal vesicle Source: UniProtKB-SubCell
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoplasmic vesicle, CytoskeletonPathology & Biotechi
Involvement in diseasei
Anencephaly (ANPH)1 Publication
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionAn extreme form of neural tube defect resulting in the absence of brain tissues, and death in utero or perinatally. Infants are born with intact spinal cords, cerebellums, and brainstems, but lack formation of neural structures above this level. The skull is only partially formed. ANPH inheritance is autosomal recessive.
Related information in OMIMFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_079581 | 518 | D → N in ANPH; decreases protein stability; leads to microtubules disruption; exhibits multipolar spindles; exhibits abnormal cytokinesis. 1 Publication | 1 |
Keywords - Diseasei
Disease variantOrganism-specific databases
DisGeNETi | 55521 |
MalaCardsi | TRIM36 |
MIMi | 206500, phenotype |
OpenTargetsi | ENSG00000152503 |
PharmGKBi | PA38111 |
Miscellaneous databases
Pharosi | Q9NQ86, Tbio |
Genetic variation databases
BioMutai | TRIM36 |
DMDMi | 313104034 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056252 | 1 – 728 | E3 ubiquitin-protein ligase TRIM36Add BLAST | 728 |
Proteomic databases
jPOSTi | Q9NQ86 |
MassIVEi | Q9NQ86 |
MaxQBi | Q9NQ86 |
PaxDbi | Q9NQ86 |
PeptideAtlasi | Q9NQ86 |
PRIDEi | Q9NQ86 |
ProteomicsDBi | 20113 82103 [Q9NQ86-1] 82104 [Q9NQ86-2] 900 |
PTM databases
iPTMneti | Q9NQ86 |
PhosphoSitePlusi | Q9NQ86 |
Expressioni
Tissue specificityi
Highly expressed in testis, prostate and brain (PubMed:15145053). Weakly expressed in kidney, lung and heart (PubMed:15145053). Expressed in fetal tissues (PubMed:28087737).2 Publications
Gene expression databases
Bgeei | ENSG00000152503, Expressed in testis and 177 other tissues |
ExpressionAtlasi | Q9NQ86, baseline and differential |
Genevisiblei | Q9NQ86, HS |
Organism-specific databases
HPAi | ENSG00000152503, Tissue enriched (testis) |
Interactioni
Subunit structurei
Interacts with CENPH.
By similarityBinary interactionsi
Hide detailsQ9NQ86
GO - Molecular functioni
- alpha-tubulin binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 120695, 22 interactors |
IntActi | Q9NQ86, 14 interactors |
STRINGi | 9606.ENSP00000282369 |
Miscellaneous databases
RNActi | Q9NQ86, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 356 – 413 | COSPROSITE-ProRule annotationAdd BLAST | 58 | |
Domaini | 419 – 510 | Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST | 92 | |
Domaini | 508 – 720 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 213 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 271 – 345 | Sequence analysisAdd BLAST | 75 |
Sequence similaritiesi
Belongs to the TRIM/RBCC family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 33 – 84 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 52 | |
Zinc fingeri | 154 – 192 | B box-type 1PROSITE-ProRule annotationAdd BLAST | 39 | |
Zinc fingeri | 207 – 249 | B box-type 2PROSITE-ProRule annotationAdd BLAST | 43 |
Keywords - Domaini
Coiled coil, Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000158373 |
HOGENOMi | CLU_013137_19_3_1 |
InParanoidi | Q9NQ86 |
OMAi | MYCELCK |
OrthoDBi | 180330at2759 |
PhylomeDBi | Q9NQ86 |
TreeFami | TF315216 |
Family and domain databases
CDDi | cd00063, FN3, 1 hit cd12894, SPRY_PRY_TRIM36, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR017903, COS_domain IPR003961, FN3_dom IPR036116, FN3_sf IPR013783, Ig-like_fold IPR040859, Midline-1_COS IPR035727, SPRY/PRY_TRIM36 IPR027726, Trim36 IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR017907, Znf_RING_CS |
PANTHERi | PTHR24099:SF18, PTHR24099:SF18, 1 hit |
Pfami | View protein in Pfam PF18568, COS, 1 hit PF00041, fn3, 1 hit PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS51262, COS, 1 hit PS50853, FN3, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9NQ86-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSESGEMSEF GYIMELIAKG KVTIKNIERE LICPACKELF THPLILPCQH
60 70 80 90 100
SICHKCVKEL LLTLDDSFND VGSDNSNQSS PRLRLPSPSM DKIDRINRPG
110 120 130 140 150
WKRNSLTPRT TVFPCPGCEH DVDLGERGIN GLFRNFTLET IVERYRQAAR
160 170 180 190 200
AATAIMCDLC KPPPQESTKS CMDCSASYCN ECFKIHHPWG TIKAQHEYVG
210 220 230 240 250
PTTNFRPKIL MCPEHETERI NMYCELCRRP VCHLCKLGGN HANHRVTTMS
260 270 280 290 300
SAYKTLKEKL SKDIDYLIGK ESQVKSQISE LNLLMKETEC NGERAKEEAI
310 320 330 340 350
THFEKLFEVL EERKSSVLKA IDSSKKLRLD KFQTQMEEYQ GLLENNGLVG
360 370 380 390 400
YAQEVLKETD QSCFVQTAKQ LHLRIQKATE SLKSFRPAAQ TSFEDYVVNT
410 420 430 440 450
SKQTELLGEL SFFSSGIDVP EINEEQSKVY NNALINWHHP EKDKADSYVL
460 470 480 490 500
EYRKINRDDE MSWNEIEVCG TSKIIQDLEN SSTYAFRVRA YKGSICSPCS
510 520 530 540 550
RELILHTPPA PVFSFLFDEK CGYNNEHLLL NLKRDRVESR AGFNLLLAAE
560 570 580 590 600
RIQVGYYTSL DYIIGDTGIT KGKHFWAFRV EPYSYLVKVG VASSDKLQEW
610 620 630 640 650
LRSPRDAVSP RYEQDSGHDS GSEDACFDSS QPFTLVTIGM QKFFIPKSPT
660 670 680 690 700
SSNEPENRVL PMPTSIGIFL DCDKGKVDFY DMDQMKCLYE RQVDCSHTLY
710 720
PAFALMGSGG IQLEEPITAK YLEYQEDM
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9PBG3 | E9PBG3_HUMAN | E3 ubiquitin-protein ligase TRIM36 | TRIM36 | 573 | Annotation score: | ||
D6RAU9 | D6RAU9_HUMAN | E3 ubiquitin-protein ligase TRIM36 | TRIM36 | 170 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 313 | R → G in BAH12340 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_023197 | 428 | K → R2 PublicationsCorresponds to variant dbSNP:rs79290430Ensembl. | 1 | |
Natural variantiVAR_023198 | 456 | N → S1 PublicationCorresponds to variant dbSNP:rs17137481Ensembl. | 1 | |
Natural variantiVAR_079581 | 518 | D → N in ANPH; decreases protein stability; leads to microtubules disruption; exhibits multipolar spindles; exhibits abnormal cytokinesis. 1 Publication | 1 | |
Natural variantiVAR_020490 | 678 | D → N4 PublicationsCorresponds to variant dbSNP:rs2974617Ensembl. | 1 | |
Natural variantiVAR_057221 | 725 | Q → E. Corresponds to variant dbSNP:rs3749745Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_053818 | 1 – 21 | MSESG…IAKGK → MEGDGSDSP in isoform 4. 1 PublicationAdd BLAST | 21 | |
Alternative sequenceiVSP_045791 | 22 – 61 | VTIKN…VKELL → ASAMGLQQTHEHSRLTSKGG EARCPFEISEVGKQSLPRRT in isoform 3. 1 PublicationAdd BLAST | 40 | |
Alternative sequenceiVSP_043511 | 22 – 60 | VTIKN…CVKEL → MPDWRRGYRCRQGCGKTTEL ATATDFSQTGNKSGKHFKT in isoform 2. 1 PublicationAdd BLAST | 39 | |
Alternative sequenceiVSP_043512 | 61 – 728 | Missing in isoform 2. 1 PublicationAdd BLAST | 668 | |
Alternative sequenceiVSP_045792 | 62 – 728 | Missing in isoform 3. 1 PublicationAdd BLAST | 667 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ272269 mRNA Translation: CAB94831.1 BX460627 mRNA No translation available. AK316422 mRNA Translation: BAH14793.1 AK296389 mRNA Translation: BAH12340.1 AC008494 Genomic DNA No translation available. AC094104 Genomic DNA No translation available. CH471086 Genomic DNA Translation: EAW48972.1 BC045164 mRNA Translation: AAH45164.1 BC046096 mRNA Translation: AAH46096.1 BC130334 mRNA Translation: AAI30335.1 |
CCDSi | CCDS34211.1 [Q9NQ86-2] CCDS34212.1 [Q9NQ86-3] CCDS4115.1 [Q9NQ86-1] CCDS75287.1 [Q9NQ86-4] |
RefSeqi | NP_001017397.1, NM_001017397.1 [Q9NQ86-2] NP_001017398.1, NM_001017398.1 [Q9NQ86-3] NP_001287681.1, NM_001300752.1 NP_001287688.1, NM_001300759.1 [Q9NQ86-4] NP_061170.2, NM_018700.3 [Q9NQ86-1] |
Genome annotation databases
Ensembli | ENST00000282369; ENSP00000282369; ENSG00000152503 [Q9NQ86-1] ENST00000379617; ENSP00000368937; ENSG00000152503 [Q9NQ86-3] ENST00000379618; ENSP00000368938; ENSG00000152503 [Q9NQ86-2] ENST00000513154; ENSP00000423934; ENSG00000152503 [Q9NQ86-4] |
GeneIDi | 55521 |
KEGGi | hsa:55521 |
UCSCi | uc003kqs.4, human [Q9NQ86-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ272269 mRNA Translation: CAB94831.1 BX460627 mRNA No translation available. AK316422 mRNA Translation: BAH14793.1 AK296389 mRNA Translation: BAH12340.1 AC008494 Genomic DNA No translation available. AC094104 Genomic DNA No translation available. CH471086 Genomic DNA Translation: EAW48972.1 BC045164 mRNA Translation: AAH45164.1 BC046096 mRNA Translation: AAH46096.1 BC130334 mRNA Translation: AAI30335.1 |
CCDSi | CCDS34211.1 [Q9NQ86-2] CCDS34212.1 [Q9NQ86-3] CCDS4115.1 [Q9NQ86-1] CCDS75287.1 [Q9NQ86-4] |
RefSeqi | NP_001017397.1, NM_001017397.1 [Q9NQ86-2] NP_001017398.1, NM_001017398.1 [Q9NQ86-3] NP_001287681.1, NM_001300752.1 NP_001287688.1, NM_001300759.1 [Q9NQ86-4] NP_061170.2, NM_018700.3 [Q9NQ86-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 120695, 22 interactors |
IntActi | Q9NQ86, 14 interactors |
STRINGi | 9606.ENSP00000282369 |
PTM databases
iPTMneti | Q9NQ86 |
PhosphoSitePlusi | Q9NQ86 |
Genetic variation databases
BioMutai | TRIM36 |
DMDMi | 313104034 |
Proteomic databases
jPOSTi | Q9NQ86 |
MassIVEi | Q9NQ86 |
MaxQBi | Q9NQ86 |
PaxDbi | Q9NQ86 |
PeptideAtlasi | Q9NQ86 |
PRIDEi | Q9NQ86 |
ProteomicsDBi | 20113 82103 [Q9NQ86-1] 82104 [Q9NQ86-2] 900 |
Protocols and materials databases
Antibodypediai | 25412, 231 antibodies |
Genome annotation databases
Ensembli | ENST00000282369; ENSP00000282369; ENSG00000152503 [Q9NQ86-1] ENST00000379617; ENSP00000368937; ENSG00000152503 [Q9NQ86-3] ENST00000379618; ENSP00000368938; ENSG00000152503 [Q9NQ86-2] ENST00000513154; ENSP00000423934; ENSG00000152503 [Q9NQ86-4] |
GeneIDi | 55521 |
KEGGi | hsa:55521 |
UCSCi | uc003kqs.4, human [Q9NQ86-1] |
Organism-specific databases
CTDi | 55521 |
DisGeNETi | 55521 |
GeneCardsi | TRIM36 |
HGNCi | HGNC:16280, TRIM36 |
HPAi | ENSG00000152503, Tissue enriched (testis) |
MalaCardsi | TRIM36 |
MIMi | 206500, phenotype 609317, gene |
neXtProti | NX_Q9NQ86 |
OpenTargetsi | ENSG00000152503 |
PharmGKBi | PA38111 |
VEuPathDBi | HostDB:ENSG00000152503.9 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2177, Eukaryota |
GeneTreei | ENSGT00940000158373 |
HOGENOMi | CLU_013137_19_3_1 |
InParanoidi | Q9NQ86 |
OMAi | MYCELCK |
OrthoDBi | 180330at2759 |
PhylomeDBi | Q9NQ86 |
TreeFami | TF315216 |
Enzyme and pathway databases
PathwayCommonsi | Q9NQ86 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 55521, 2 hits in 981 CRISPR screens |
ChiTaRSi | TRIM36, human |
GenomeRNAii | 55521 |
Pharosi | Q9NQ86, Tbio |
PROi | PR:Q9NQ86 |
RNActi | Q9NQ86, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000152503, Expressed in testis and 177 other tissues |
ExpressionAtlasi | Q9NQ86, baseline and differential |
Genevisiblei | Q9NQ86, HS |
Family and domain databases
CDDi | cd00063, FN3, 1 hit cd12894, SPRY_PRY_TRIM36, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 2.60.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR003879, Butyrophylin_SPRY IPR013320, ConA-like_dom_sf IPR017903, COS_domain IPR003961, FN3_dom IPR036116, FN3_sf IPR013783, Ig-like_fold IPR040859, Midline-1_COS IPR035727, SPRY/PRY_TRIM36 IPR027726, Trim36 IPR027370, Znf-RING_LisH IPR000315, Znf_B-box IPR001841, Znf_RING IPR017907, Znf_RING_CS |
PANTHERi | PTHR24099:SF18, PTHR24099:SF18, 1 hit |
Pfami | View protein in Pfam PF18568, COS, 1 hit PF00041, fn3, 1 hit PF00643, zf-B_box, 1 hit PF13445, zf-RING_UBOX, 1 hit |
PRINTSi | PR01407, BUTYPHLNCDUF |
SMARTi | View protein in SMART SM00336, BBOX, 1 hit SM00184, RING, 1 hit |
SUPFAMi | SSF49265, SSF49265, 1 hit SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS51262, COS, 1 hit PS50853, FN3, 1 hit PS50119, ZF_BBOX, 1 hit PS00518, ZF_RING_1, 1 hit PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TRI36_HUMAN | |
Accessioni | Q9NQ86Primary (citable) accession number: Q9NQ86 Secondary accession number(s): A1L3Z1 Q0P5Z9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | November 30, 2010 | |
Last modified: | April 7, 2021 | |
This is version 182 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 5
Human chromosome 5: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families