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Protein

Synembryn-A

Gene

RIC8A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.By similarity1 Publication

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: GO_Central
  • GTPase activator activity Source: GO_Central
  • guanyl-nucleotide exchange factor activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Synembryn-A
Alternative name(s):
Protein Ric-8A
Gene namesi
Name:RIC8A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000177963.12
HGNCiHGNC:29550 RIC8A
MIMi609146 gene
neXtProtiNX_Q9NPQ8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000177963
PharmGKBiPA142671067

Polymorphism and mutation databases

DMDMi308153562

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002358901 – 531Synembryn-AAdd BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei436PhosphoserineCombined sources1
Modified residuei441PhosphothreonineCombined sources1
Modified residuei443PhosphothreonineBy similarity1
Modified residuei502PhosphoserineCombined sources1
Modified residuei523PhosphoserineCombined sources1
Modified residuei524PhosphoserineCombined sources1
Modified residuei528PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9NPQ8
MaxQBiQ9NPQ8
PaxDbiQ9NPQ8
PeptideAtlasiQ9NPQ8
PRIDEiQ9NPQ8
ProteomicsDBi82040
82041 [Q9NPQ8-2]
82042 [Q9NPQ8-3]
82043 [Q9NPQ8-4]

PTM databases

iPTMnetiQ9NPQ8
PhosphoSitePlusiQ9NPQ8

Expressioni

Gene expression databases

BgeeiENSG00000177963 Expressed in 220 organ(s), highest expression level in testis
ExpressionAtlasiQ9NPQ8 baseline and differential
GenevisibleiQ9NPQ8 HS

Organism-specific databases

HPAiCAB046012
HPA041491

Interactioni

Subunit structurei

Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity. Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma. Interacts (via C-terminus) with RGS14; the interaction stimulates the dissociation of the complex between RGS14 and the active GTP-bound form of GNAI1 (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121946, 45 interactors
IntActiQ9NPQ8, 22 interactors
MINTiQ9NPQ8
STRINGi9606.ENSP00000325941

Structurei

3D structure databases

ProteinModelPortaliQ9NPQ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the synembryn family.Curated

Phylogenomic databases

eggNOGiKOG4464 Eukaryota
ENOG410XSCJ LUCA
GeneTreeiENSGT00390000014700
HOVERGENiHBG054867
InParanoidiQ9NPQ8
OMAiGMSPRGH
OrthoDBiEOG091G07N6
PhylomeDBiQ9NPQ8
TreeFamiTF314907

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR019318 Gua_nucleotide_exch_fac_Ric8
IPR008376 Synembryn
PANTHERiPTHR12425 PTHR12425, 1 hit
PfamiView protein in Pfam
PF10165 Ric8, 1 hit
PRINTSiPR01802 SYNEMBRYN
SUPFAMiSSF48371 SSF48371, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NPQ8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEPRAVAEAV ETGEEDVIME ALRSYNQEHS QSFTFDDAQQ EDRKRLAELL
60 70 80 90 100
VSVLEQGLPP SHRVIWLQSV RILSRDRNCL DPFTSRQSLQ ALACYADISV
110 120 130 140 150
SEGSVPESAD MDVVLESLKC LCNLVLSSPV AQMLAAEARL VVKLTERVGL
160 170 180 190 200
YRERSFPHDV QFFDLRLLFL LTALRTDVRQ QLFQELKGVR LLTDTLELTL
210 220 230 240 250
GVTPEGNPPP TLLPSQETER AMEILKVLFN ITLDSIKGEV DEEDAALYRH
260 270 280 290 300
LGTLLRHCVM IATAGDRTEE FHGHAVNLLG NLPLKCLDVL LTLEPHGDST
310 320 330 340 350
EFMGVNMDVI RALLIFLEKR LHKTHRLKES VAPVLSVLTE CARMHRPARK
360 370 380 390 400
FLKAQVLPPL RDVRTRPEVG EMLRNKLVRL MTHLDTDVKR VAAEFLFVLC
410 420 430 440 450
SESVPRFIKY TGYGNAAGLL AARGLMAGGR PEGQYSEDED TDTDEYKEAK
460 470 480 490 500
ASINPVTGRV EEKPPNPMEG MTEEQKEHEA MKLVTMFDKL SRNRVIQPMG
510 520 530
MSPRGHLTSL QDAMCETMEQ QLSSDPDSDP D
Length:531
Mass (Da):59,710
Last modified:October 5, 2010 - v3
Checksum:i2BAF3E2791F6E021
GO
Isoform 2 (identifier: Q9NPQ8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: MEPRAVAEAV...FDDAQQEDRK → MMPNRRTGRW...WWSRSSPCPQ

Show »
Length:525
Mass (Da):58,943
Checksum:i823D0B28817F594D
GO
Isoform 3 (identifier: Q9NPQ8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     354-354: A → AQGWPPP

Show »
Length:537
Mass (Da):60,372
Checksum:iC46E2F66B44DE40D
GO
Isoform 4 (identifier: Q9NPQ8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     209-209: Missing.

Show »
Length:530
Mass (Da):59,612
Checksum:i46ED334ED07C9EA8
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PSI0E9PSI0_HUMAN
Synembryn-A
RIC8A
183Annotation score:
H0YC88H0YC88_HUMAN
Synembryn-A
RIC8A
147Annotation score:
E9PMP0E9PMP0_HUMAN
Synembryn-A
RIC8A
44Annotation score:
E9PI04E9PI04_HUMAN
Synembryn-A
RIC8A
148Annotation score:
H0YE35H0YE35_HUMAN
Synembryn-A
RIC8A
147Annotation score:
H0YEN0H0YEN0_HUMAN
Synembryn-A
RIC8A
286Annotation score:
E9PLE5E9PLE5_HUMAN
Synembryn-A
RIC8A
52Annotation score:

Sequence cautioni

The sequence BAB14282 differs from that shown. Reason: Frameshift at position 397.Curated
The sequence BAB14282 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB15653 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB55126 differs from that shown. Reason: Frameshift at position 48.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48E → V in BAB55126 (PubMed:14702039).Curated1
Sequence conflicti405P → L in CAD98025 (PubMed:17974005).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0185071 – 44MEPRA…QEDRK → MMPNRRTGRWVLAQGVKGQG RVAPGRRAWWSRSSPCPQ in isoform 2. 1 PublicationAdd BLAST44
Alternative sequenceiVSP_039849209Missing in isoform 4. 1 Publication1
Alternative sequenceiVSP_018508354A → AQGWPPP in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136935 mRNA Translation: CAB66869.1
AK022870 mRNA Translation: BAB14282.1 Sequence problems.
AK027090 mRNA Translation: BAB15653.1 Different initiation.
AK027461 mRNA Translation: BAB55126.1 Frameshift.
AL390088 mRNA Translation: CAB98211.1
BX538115 mRNA Translation: CAD98025.1
AC069287 Genomic DNA No translation available.
BC011821 mRNA Translation: AAH11821.2
BC111499 mRNA Translation: AAI11500.1
BC121807 mRNA Translation: AAI21808.1
BC121808 mRNA Translation: AAI21809.1
CCDSiCCDS65982.1 [Q9NPQ8-1]
CCDS7690.1 [Q9NPQ8-3]
RefSeqiNP_001273063.1, NM_001286134.1 [Q9NPQ8-1]
NP_068751.4, NM_021932.5 [Q9NPQ8-3]
XP_016873596.1, XM_017018107.1
UniGeneiHs.592292

Genome annotation databases

EnsembliENST00000325207; ENSP00000325941; ENSG00000177963 [Q9NPQ8-3]
ENST00000526104; ENSP00000432008; ENSG00000177963 [Q9NPQ8-1]
ENST00000527696; ENSP00000434833; ENSG00000177963 [Q9NPQ8-2]
GeneIDi60626
KEGGihsa:60626
UCSCiuc001lof.5 human [Q9NPQ8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL136935 mRNA Translation: CAB66869.1
AK022870 mRNA Translation: BAB14282.1 Sequence problems.
AK027090 mRNA Translation: BAB15653.1 Different initiation.
AK027461 mRNA Translation: BAB55126.1 Frameshift.
AL390088 mRNA Translation: CAB98211.1
BX538115 mRNA Translation: CAD98025.1
AC069287 Genomic DNA No translation available.
BC011821 mRNA Translation: AAH11821.2
BC111499 mRNA Translation: AAI11500.1
BC121807 mRNA Translation: AAI21808.1
BC121808 mRNA Translation: AAI21809.1
CCDSiCCDS65982.1 [Q9NPQ8-1]
CCDS7690.1 [Q9NPQ8-3]
RefSeqiNP_001273063.1, NM_001286134.1 [Q9NPQ8-1]
NP_068751.4, NM_021932.5 [Q9NPQ8-3]
XP_016873596.1, XM_017018107.1
UniGeneiHs.592292

3D structure databases

ProteinModelPortaliQ9NPQ8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121946, 45 interactors
IntActiQ9NPQ8, 22 interactors
MINTiQ9NPQ8
STRINGi9606.ENSP00000325941

PTM databases

iPTMnetiQ9NPQ8
PhosphoSitePlusiQ9NPQ8

Polymorphism and mutation databases

DMDMi308153562

Proteomic databases

EPDiQ9NPQ8
MaxQBiQ9NPQ8
PaxDbiQ9NPQ8
PeptideAtlasiQ9NPQ8
PRIDEiQ9NPQ8
ProteomicsDBi82040
82041 [Q9NPQ8-2]
82042 [Q9NPQ8-3]
82043 [Q9NPQ8-4]

Protocols and materials databases

DNASUi60626
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325207; ENSP00000325941; ENSG00000177963 [Q9NPQ8-3]
ENST00000526104; ENSP00000432008; ENSG00000177963 [Q9NPQ8-1]
ENST00000527696; ENSP00000434833; ENSG00000177963 [Q9NPQ8-2]
GeneIDi60626
KEGGihsa:60626
UCSCiuc001lof.5 human [Q9NPQ8-1]

Organism-specific databases

CTDi60626
EuPathDBiHostDB:ENSG00000177963.12
GeneCardsiRIC8A
HGNCiHGNC:29550 RIC8A
HPAiCAB046012
HPA041491
MIMi609146 gene
neXtProtiNX_Q9NPQ8
OpenTargetsiENSG00000177963
PharmGKBiPA142671067
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4464 Eukaryota
ENOG410XSCJ LUCA
GeneTreeiENSGT00390000014700
HOVERGENiHBG054867
InParanoidiQ9NPQ8
OMAiGMSPRGH
OrthoDBiEOG091G07N6
PhylomeDBiQ9NPQ8
TreeFamiTF314907

Miscellaneous databases

ChiTaRSiRIC8A human
GeneWikiiRIC8A
GenomeRNAii60626
PROiPR:Q9NPQ8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000177963 Expressed in 220 organ(s), highest expression level in testis
ExpressionAtlasiQ9NPQ8 baseline and differential
GenevisibleiQ9NPQ8 HS

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR019318 Gua_nucleotide_exch_fac_Ric8
IPR008376 Synembryn
PANTHERiPTHR12425 PTHR12425, 1 hit
PfamiView protein in Pfam
PF10165 Ric8, 1 hit
PRINTSiPR01802 SYNEMBRYN
SUPFAMiSSF48371 SSF48371, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIC8A_HUMAN
AccessioniPrimary (citable) accession number: Q9NPQ8
Secondary accession number(s): Q0P508
, Q2T9J1, Q7Z352, Q96EZ1, Q96SZ2, Q9H064, Q9H5H3, Q9H9E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: October 5, 2010
Last modified: November 7, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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