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Protein

Mediator of RNA polymerase II transcription subunit 4

Gene

MED4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 4
Alternative name(s):
Activator-recruited cofactor 36 kDa component
Short name:
ARC36
Mediator complex subunit 4
TRAP/SMCC/PC2 subunit p36 subunit
Vitamin D3 receptor-interacting protein complex 36 kDa component
Short name:
DRIP36
Gene namesi
Name:MED4
Synonyms:ARC36, DRIP36, VDRIP
ORF Names:HSPC126
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 13

Organism-specific databases

EuPathDBiHostDB:ENSG00000136146.14
HGNCiHGNC:17903 MED4
MIMi605718 gene
neXtProtiNX_Q9NPJ6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi29079
OpenTargetsiENSG00000136146
PharmGKBiPA134877001

Polymorphism and mutation databases

DMDMi29840770

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000963832 – 270Mediator of RNA polymerase II transcription subunit 4Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei32PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9NPJ6
MaxQBiQ9NPJ6
PaxDbiQ9NPJ6
PeptideAtlasiQ9NPJ6
PRIDEiQ9NPJ6
ProteomicsDBi82028

PTM databases

iPTMnetiQ9NPJ6
PhosphoSitePlusiQ9NPJ6

Expressioni

Gene expression databases

BgeeiENSG00000136146 Expressed in 225 organ(s), highest expression level in testis
CleanExiHS_MED4
ExpressionAtlasiQ9NPJ6 baseline and differential
GenevisibleiQ9NPJ6 HS

Organism-specific databases

HPAiHPA006232

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118849, 374 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9NPJ6
IntActiQ9NPJ6, 90 interactors
MINTiQ9NPJ6
STRINGi9606.ENSP00000258648

Structurei

3D structure databases

ProteinModelPortaliQ9NPJ6
SMRiQ9NPJ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili24 – 48Sequence analysisAdd BLAST25
Coiled coili90 – 131Sequence analysisAdd BLAST42

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi262 – 269Poly-Ser8

Sequence similaritiesi

Belongs to the Mediator complex subunit 4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4552 Eukaryota
ENOG410XQ30 LUCA
GeneTreeiENSGT00390000012063
HOGENOMiHOG000043883
HOVERGENiHBG052450
InParanoidiQ9NPJ6
KOiK15146
OMAiPPHHGND
OrthoDBiEOG091G0M8S
PhylomeDBiQ9NPJ6
TreeFamiTF324421

Family and domain databases

InterProiView protein in InterPro
IPR019258 Mediator_Med4
PANTHERiPTHR13208 PTHR13208, 1 hit
PfamiView protein in Pfam
PF10018 Med4, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9NPJ6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI
60 70 80 90 100
SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV
110 120 130 140 150
EKRDSDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK
160 170 180 190 200
YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG
210 220 230 240 250
HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE
260 270
NEDDVEIMST DSSSSSSESD
Length:270
Mass (Da):29,745
Last modified:October 1, 2000 - v1
Checksum:i05641357EBFA85F4
GO
Isoform 2 (identifier: Q9NPJ6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Show »
Length:224
Mass (Da):24,947
Checksum:i0598148BFB738B06
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5T911Q5T911_HUMAN
Mediator of RNA polymerase II trans...
MED4
238Annotation score:
A0A087X0U0A0A087X0U0_HUMAN
Mediator of RNA polymerase II trans...
MED4
153Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105S → G in AAH05189 (PubMed:15489334).Curated1
Sequence conflicti120I → T in CAG46629 (Ref. 5) Curated1
Sequence conflicti127Q → R in BAD96737 (Ref. 6) Curated1
Sequence conflicti152A → P in CAG33557 (Ref. 5) Curated1
Sequence conflicti183R → K in AAF37289 (PubMed:10882111).Curated1
Sequence conflicti218P → S in BAD96433 (Ref. 6) Curated1
Sequence conflicti251N → D in AAH05189 (PubMed:15489334).Curated1
Sequence conflicti265S → T in AAF37289 (PubMed:10882111).Curated1
Sequence conflicti265S → T in CAG46629 (Ref. 5) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0470721 – 46Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300618 mRNA Translation: AAG22542.1
AF230381 mRNA Translation: AAF37289.1
AF161475 mRNA Translation: AAF29090.1
AK001934 mRNA Translation: BAA91987.1
CR457276 mRNA Translation: CAG33557.1
CR541830 mRNA Translation: CAG46629.1
AK222713 mRNA Translation: BAD96433.1
AK301835 mRNA Translation: BAG63279.1
AK223017 mRNA Translation: BAD96737.1
AL158196 Genomic DNA No translation available.
BC005189 mRNA Translation: AAH05189.1
CCDSiCCDS59241.1 [Q9NPJ6-2]
CCDS9408.1 [Q9NPJ6-1]
RefSeqiNP_001257558.1, NM_001270629.1 [Q9NPJ6-2]
NP_054885.1, NM_014166.3 [Q9NPJ6-1]
UniGeneiHs.181112
Hs.741275

Genome annotation databases

EnsembliENST00000258648; ENSP00000258648; ENSG00000136146 [Q9NPJ6-1]
ENST00000378586; ENSP00000367849; ENSG00000136146 [Q9NPJ6-2]
GeneIDi29079
KEGGihsa:29079
UCSCiuc001vby.3 human [Q9NPJ6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF300618 mRNA Translation: AAG22542.1
AF230381 mRNA Translation: AAF37289.1
AF161475 mRNA Translation: AAF29090.1
AK001934 mRNA Translation: BAA91987.1
CR457276 mRNA Translation: CAG33557.1
CR541830 mRNA Translation: CAG46629.1
AK222713 mRNA Translation: BAD96433.1
AK301835 mRNA Translation: BAG63279.1
AK223017 mRNA Translation: BAD96737.1
AL158196 Genomic DNA No translation available.
BC005189 mRNA Translation: AAH05189.1
CCDSiCCDS59241.1 [Q9NPJ6-2]
CCDS9408.1 [Q9NPJ6-1]
RefSeqiNP_001257558.1, NM_001270629.1 [Q9NPJ6-2]
NP_054885.1, NM_014166.3 [Q9NPJ6-1]
UniGeneiHs.181112
Hs.741275

3D structure databases

ProteinModelPortaliQ9NPJ6
SMRiQ9NPJ6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118849, 374 interactors
ComplexPortaliCPX-3227 Core mediator complex
CORUMiQ9NPJ6
IntActiQ9NPJ6, 90 interactors
MINTiQ9NPJ6
STRINGi9606.ENSP00000258648

PTM databases

iPTMnetiQ9NPJ6
PhosphoSitePlusiQ9NPJ6

Polymorphism and mutation databases

DMDMi29840770

Proteomic databases

EPDiQ9NPJ6
MaxQBiQ9NPJ6
PaxDbiQ9NPJ6
PeptideAtlasiQ9NPJ6
PRIDEiQ9NPJ6
ProteomicsDBi82028

Protocols and materials databases

DNASUi29079
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000258648; ENSP00000258648; ENSG00000136146 [Q9NPJ6-1]
ENST00000378586; ENSP00000367849; ENSG00000136146 [Q9NPJ6-2]
GeneIDi29079
KEGGihsa:29079
UCSCiuc001vby.3 human [Q9NPJ6-1]

Organism-specific databases

CTDi29079
DisGeNETi29079
EuPathDBiHostDB:ENSG00000136146.14
GeneCardsiMED4
HGNCiHGNC:17903 MED4
HPAiHPA006232
MIMi605718 gene
neXtProtiNX_Q9NPJ6
OpenTargetsiENSG00000136146
PharmGKBiPA134877001
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4552 Eukaryota
ENOG410XQ30 LUCA
GeneTreeiENSGT00390000012063
HOGENOMiHOG000043883
HOVERGENiHBG052450
InParanoidiQ9NPJ6
KOiK15146
OMAiPPHHGND
OrthoDBiEOG091G0M8S
PhylomeDBiQ9NPJ6
TreeFamiTF324421

Enzyme and pathway databases

ReactomeiR-HSA-1989781 PPARA activates gene expression
R-HSA-212436 Generic Transcription Pathway
R-HSA-381340 Transcriptional regulation of white adipocyte differentiation

Miscellaneous databases

ChiTaRSiMED4 human
GeneWikiiMED4
GenomeRNAii29079
PROiPR:Q9NPJ6
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136146 Expressed in 225 organ(s), highest expression level in testis
CleanExiHS_MED4
ExpressionAtlasiQ9NPJ6 baseline and differential
GenevisibleiQ9NPJ6 HS

Family and domain databases

InterProiView protein in InterPro
IPR019258 Mediator_Med4
PANTHERiPTHR13208 PTHR13208, 1 hit
PfamiView protein in Pfam
PF10018 Med4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED4_HUMAN
AccessioniPrimary (citable) accession number: Q9NPJ6
Secondary accession number(s): B4DX67
, Q53GB4, Q53H68, Q5T912, Q6FHC4, Q6IA79, Q9BS95, Q9NYR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 13
    Human chromosome 13: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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