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Protein

Acyl-coenzyme A thioesterase 13

Gene

ACOT13

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).By similarity2 Publications

Kineticsi

  1. KM=4.9 µM for n-decanoyl-CoA1 Publication
  2. KM=26 µM for n-octanoyl-CoA1 Publication
  3. KM=5 µM for myristoyl-CoA1 Publication
  4. KM=9 µM for palmitoyl-CoA1 Publication
  5. KM=9 µM for oleoyl-CoA1 Publication
  6. KM=10 µM for 3,4-dihydroxyphenylacetyl-CoA1 Publication
  7. KM=40 µM for 3-hydroxyphenylacetyl-CoA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei50Substrate1

    GO - Molecular functioni

    • acyl-CoA hydrolase activity Source: UniProtKB

    GO - Biological processi

    • acyl-CoA metabolic process Source: Reactome
    • negative regulation of cold-induced thermogenesis Source: YuBioLab
    • protein homotetramerization Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    BRENDAi3.1.2.20 2681
    ReactomeiR-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
    SABIO-RKiQ9NPJ3

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A thioesterase 13 (EC:3.1.2.-)
    Short name:
    Acyl-CoA thioesterase 13
    Alternative name(s):
    Thioesterase superfamily member 2
    Cleaved into the following chain:
    Gene namesi
    Name:ACOT13
    Synonyms:THEM2
    ORF Names:HT012, PNAS-27
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 6

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000112304.10
    HGNCiHGNC:20999 ACOT13
    MIMi615652 gene
    neXtProtiNX_Q9NPJ3

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi50N → A: Reduced activity. 1 Publication1
    Mutagenesisi56H → A: Decreases affinity for substrate. 1 Publication1
    Mutagenesisi65D → A: Loss of activity. 2 Publications1
    Mutagenesisi65D → E or N: Reduced activity. 2 Publications1
    Mutagenesisi83S → A: Reduced activity. 1 Publication1

    Organism-specific databases

    DisGeNETi55856
    OpenTargetsiENSG00000112304
    PharmGKBiPA165617655

    Chemistry databases

    DrugBankiDB08688 undecan-2-one

    Polymorphism and mutation databases

    BioMutaiACOT13

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001566971 – 140Acyl-coenzyme A thioesterase 13Add BLAST140
    Initiator methionineiRemoved; alternateCombined sources
    ChainiPRO_00004245012 – 140Acyl-coenzyme A thioesterase 13, N-terminally processedAdd BLAST139

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1N-acetylmethionineCombined sources1
    Modified residuei2N-acetylthreonine; in Acyl-coenzyme A thioesterase 13, N-terminally processedCombined sources1
    Modified residuei27N6-acetyllysineBy similarity1
    Modified residuei37N6-acetyllysineBy similarity1
    Modified residuei43N6-acetyllysineBy similarity1
    Modified residuei108N6-acetyllysineBy similarity1
    Modified residuei127N6-acetyllysineBy similarity1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    EPDiQ9NPJ3
    MaxQBiQ9NPJ3
    PaxDbiQ9NPJ3
    PeptideAtlasiQ9NPJ3
    PRIDEiQ9NPJ3
    ProteomicsDBi82025
    TopDownProteomicsiQ9NPJ3-1 [Q9NPJ3-1]

    PTM databases

    iPTMnetiQ9NPJ3
    PhosphoSitePlusiQ9NPJ3

    Expressioni

    Gene expression databases

    BgeeiENSG00000112304 Expressed in 220 organ(s), highest expression level in nephron tubule
    CleanExiHS_THEM2
    GenevisibleiQ9NPJ3 HS

    Organism-specific databases

    HPAiHPA019881

    Interactioni

    Subunit structurei

    Homotetramer.2 Publications

    Protein-protein interaction databases

    BioGridi120958, 25 interactors
    IntActiQ9NPJ3, 7 interactors
    STRINGi9606.ENSP00000230048

    Structurei

    Secondary structure

    1140
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ9NPJ3
    SMRiQ9NPJ3
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9NPJ3

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thioesterase PaaI family.Curated

    Phylogenomic databases

    eggNOGiKOG3328 Eukaryota
    COG2050 LUCA
    GeneTreeiENSGT00390000013934
    HOGENOMiHOG000170540
    InParanoidiQ9NPJ3
    KOiK17362
    OMAiKMGTLHG
    OrthoDBiEOG091G125K
    PhylomeDBiQ9NPJ3
    TreeFamiTF315062

    Family and domain databases

    InterProiView protein in InterPro
    IPR039298 ACOT13
    IPR029069 HotDog_dom_sf
    IPR003736 PAAI_dom
    IPR006683 Thioestr_dom
    PANTHERiPTHR21660 PTHR21660, 1 hit
    PfamiView protein in Pfam
    PF03061 4HBT, 1 hit
    SUPFAMiSSF54637 SSF54637, 1 hit
    TIGRFAMsiTIGR00369 unchar_dom_1, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
    Isoform 1 (identifier: Q9NPJ3-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN
    60 70 80 90 100
    AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI
    110 120 130 140
    VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN
    Length:140
    Mass (Da):14,960
    Last modified:October 1, 2000 - v1
    Checksum:i084E36EFEE715A18
    GO
    Isoform 2 (identifier: Q9NPJ3-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-26: MTSMTQSLREVIKAMTKARNFERVLG → MVR

    Note: No experimental confirmation available.
    Show »
    Length:117
    Mass (Da):12,366
    Checksum:iA6F50AA63A00583A
    GO

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_0461011 – 26MTSMT…ERVLG → MVR in isoform 2. 1 PublicationAdd BLAST26

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF155649 mRNA Translation: AAF67006.1
    AF220186 mRNA Translation: AAF67651.1
    AF274952 mRNA Translation: AAK07529.1
    AK000508 mRNA Translation: BAA91215.1
    AK309738 mRNA No translation available.
    AL031775 Genomic DNA No translation available.
    BC000894 mRNA Translation: AAH00894.1
    CCDSiCCDS4558.1 [Q9NPJ3-1]
    CCDS54972.1 [Q9NPJ3-2]
    RefSeqiNP_001153566.1, NM_001160094.1 [Q9NPJ3-2]
    NP_060943.1, NM_018473.3 [Q9NPJ3-1]
    UniGeneiHs.731605

    Genome annotation databases

    EnsembliENST00000230048; ENSP00000230048; ENSG00000112304 [Q9NPJ3-1]
    ENST00000537591; ENSP00000445552; ENSG00000112304 [Q9NPJ3-2]
    GeneIDi55856
    KEGGihsa:55856
    UCSCiuc003nek.4 human [Q9NPJ3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF155649 mRNA Translation: AAF67006.1
    AF220186 mRNA Translation: AAF67651.1
    AF274952 mRNA Translation: AAK07529.1
    AK000508 mRNA Translation: BAA91215.1
    AK309738 mRNA No translation available.
    AL031775 Genomic DNA No translation available.
    BC000894 mRNA Translation: AAH00894.1
    CCDSiCCDS4558.1 [Q9NPJ3-1]
    CCDS54972.1 [Q9NPJ3-2]
    RefSeqiNP_001153566.1, NM_001160094.1 [Q9NPJ3-2]
    NP_060943.1, NM_018473.3 [Q9NPJ3-1]
    UniGeneiHs.731605

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2F0XX-ray2.30A/B/C/D/E/F/G/H1-140[»]
    2H4UX-ray2.20A/B/C/D19-140[»]
    3F5OX-ray1.70A/B/C/D/E/F/G/H1-140[»]
    ProteinModelPortaliQ9NPJ3
    SMRiQ9NPJ3
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi120958, 25 interactors
    IntActiQ9NPJ3, 7 interactors
    STRINGi9606.ENSP00000230048

    Chemistry databases

    DrugBankiDB08688 undecan-2-one

    PTM databases

    iPTMnetiQ9NPJ3
    PhosphoSitePlusiQ9NPJ3

    Polymorphism and mutation databases

    BioMutaiACOT13

    Proteomic databases

    EPDiQ9NPJ3
    MaxQBiQ9NPJ3
    PaxDbiQ9NPJ3
    PeptideAtlasiQ9NPJ3
    PRIDEiQ9NPJ3
    ProteomicsDBi82025
    TopDownProteomicsiQ9NPJ3-1 [Q9NPJ3-1]

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000230048; ENSP00000230048; ENSG00000112304 [Q9NPJ3-1]
    ENST00000537591; ENSP00000445552; ENSG00000112304 [Q9NPJ3-2]
    GeneIDi55856
    KEGGihsa:55856
    UCSCiuc003nek.4 human [Q9NPJ3-1]

    Organism-specific databases

    CTDi55856
    DisGeNETi55856
    EuPathDBiHostDB:ENSG00000112304.10
    GeneCardsiACOT13
    H-InvDBiHIX0005627
    HGNCiHGNC:20999 ACOT13
    HPAiHPA019881
    MIMi615652 gene
    neXtProtiNX_Q9NPJ3
    OpenTargetsiENSG00000112304
    PharmGKBiPA165617655
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3328 Eukaryota
    COG2050 LUCA
    GeneTreeiENSGT00390000013934
    HOGENOMiHOG000170540
    InParanoidiQ9NPJ3
    KOiK17362
    OMAiKMGTLHG
    OrthoDBiEOG091G125K
    PhylomeDBiQ9NPJ3
    TreeFamiTF315062

    Enzyme and pathway databases

    BRENDAi3.1.2.20 2681
    ReactomeiR-HSA-77289 Mitochondrial Fatty Acid Beta-Oxidation
    SABIO-RKiQ9NPJ3

    Miscellaneous databases

    ChiTaRSiACOT13 human
    EvolutionaryTraceiQ9NPJ3
    GenomeRNAii55856
    PROiPR:Q9NPJ3
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000112304 Expressed in 220 organ(s), highest expression level in nephron tubule
    CleanExiHS_THEM2
    GenevisibleiQ9NPJ3 HS

    Family and domain databases

    InterProiView protein in InterPro
    IPR039298 ACOT13
    IPR029069 HotDog_dom_sf
    IPR003736 PAAI_dom
    IPR006683 Thioestr_dom
    PANTHERiPTHR21660 PTHR21660, 1 hit
    PfamiView protein in Pfam
    PF03061 4HBT, 1 hit
    SUPFAMiSSF54637 SSF54637, 1 hit
    TIGRFAMsiTIGR00369 unchar_dom_1, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiACO13_HUMAN
    AccessioniPrimary (citable) accession number: Q9NPJ3
    Secondary accession number(s): F5H2L4, O95549
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: October 1, 2000
    Last modified: November 7, 2018
    This is version 155 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
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