UniProtKB - Q9NPE6 (SPAG4_HUMAN)
Protein
Sperm-associated antigen 4 protein
Gene
SPAG4
Organism
Homo sapiens (Human)
Status
Functioni
Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.By similarity
Caution
Although transmembrane domains are strongly predicted, they may rather represent hydrophobic globular domains associated with microtubules.Curated
GO - Molecular functioni
- protein-membrane adaptor activity Source: GO_Central
- structural molecule activity Source: ProtInc
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- nuclear envelope organization Source: GO_Central
- spermatogenesis Source: ProtInc
Keywordsi
Biological process | Differentiation, Spermatogenesis |
Enzyme and pathway databases
PathwayCommonsi | Q9NPE6 |
Names & Taxonomyi
Protein namesi | Recommended name: Sperm-associated antigen 4 proteinAlternative name(s): Outer dense fiber-associated protein SPAG4 SUN domain-containing protein 4 |
Gene namesi | Name:SPAG4 Synonyms:SUN4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000061656.9 |
HGNCi | HGNC:11214, SPAG4 |
MIMi | 603038, gene |
neXtProti | NX_Q9NPE6 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
- flagellum axoneme By similarity
Nucleus
- Nucleus envelope By similarity
- Nucleus inner membrane By similarity
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Note: In spermatids, it is localized in the transient manchette and in the axoneme of elongating spermatids and epididymal sperm (By similarity). Colocalized with SEPT12 at the nuclear periphery in round spermatids, at sperm neck in elongated spermatids and at midpiece regions in ejaculated spermatozoa (PubMed:25775403).By similarity1 Publication
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Nucleus
- meiotic nuclear membrane microtubule tethering complex Source: GO_Central
- nuclear envelope Source: GO_Central
- nuclear inner membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-KW
- integral component of membrane Source: UniProtKB-KW
- motile cilium Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 135 – 155 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 166 – 186 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Membrane, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 6676 |
OpenTargetsi | ENSG00000061656 |
PharmGKBi | PA36050 |
Miscellaneous databases
Pharosi | Q9NPE6, Tdark |
Polymorphism and mutation databases
BioMutai | SPAG4 |
DMDMi | 27805726 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218916 | 1 – 437 | Sperm-associated antigen 4 proteinAdd BLAST | 437 |
Proteomic databases
MassIVEi | Q9NPE6 |
PaxDbi | Q9NPE6 |
PeptideAtlasi | Q9NPE6 |
PRIDEi | Q9NPE6 |
ProteomicsDBi | 81980 |
PTM databases
iPTMneti | Q9NPE6 |
PhosphoSitePlusi | Q9NPE6 |
Expressioni
Tissue specificityi
Predominantly epressed in testis. Expressed in ejaculated spermatozoa (at protein level).1 Publication
Gene expression databases
Bgeei | ENSG00000061656, Expressed in pancreas and 147 other tissues |
ExpressionAtlasi | Q9NPE6, baseline and differential |
Genevisiblei | Q9NPE6, HS |
Organism-specific databases
HPAi | ENSG00000061656, Tissue enhanced (pancreas) |
Interactioni
Subunit structurei
Homodimer.
Interacts with ODF1. May associate with microtubules (By similarity).
Interacts with SUN3 and SYNE1; suggesting the formation of a spermatogenesis-specific LINC complex; a SUN domain-based heterotrimer with SUN3 may associate with SYNE1 (By similarity).
Interacts with SEPT12 and LMNB1; during spermatogenesis (PubMed:25775403).
By similarity1 PublicationBinary interactionsi
Hide detailsQ9NPE6
Protein-protein interaction databases
BioGRIDi | 112558, 25 interactors |
IntActi | Q9NPE6, 27 interactors |
STRINGi | 9606.ENSP00000363391 |
Miscellaneous databases
RNActi | Q9NPE6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 265 – 425 | SUNPROSITE-ProRule annotationAdd BLAST | 161 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 197 – 244 | Sequence analysisAdd BLAST | 48 |
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2687, Eukaryota |
GeneTreei | ENSGT00940000161566 |
HOGENOMi | CLU_043737_2_0_1 |
InParanoidi | Q9NPE6 |
OMAi | TNTAYFW |
OrthoDBi | 1569602at2759 |
PhylomeDBi | Q9NPE6 |
TreeFami | TF323915 |
Family and domain databases
Gene3Di | 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR027776, SPAG4/SUN4 IPR012919, SUN_dom |
PANTHERi | PTHR12911:SF16, PTHR12911:SF16, 1 hit |
Pfami | View protein in Pfam PF07738, Sad1_UNC, 1 hit |
PROSITEi | View protein in PROSITE PS51469, SUN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9NPE6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRRSSRPGSA SSSRKHTPNF FSENSSMSIT SEDSKGLRSA EPGPGEPEGR
60 70 80 90 100
RARGPSCGEP ALSAGVPGGT TWAGSSQQKP APRSHNWQTA CGAATVRGGA
110 120 130 140 150
SEPTGSPVVS EEPLDLLPTL DLRQEMPPPR VFKSFLSLLF QGLSVLLSLA
160 170 180 190 200
GDVLVSMYRE VCSIRFLFTA VSLLSLFLSA FWLGLLYLVS PLENEPKEML
210 220 230 240 250
TLSEYHERVR SQGQQLQQLQ AELDKLHKEV STVRAANSER VAKLVFQRLN
260 270 280 290 300
EDFVRKPDYA LSSVGASIDL QKTSHDYADR NTAYFWNRFS FWNYARPPTV
310 320 330 340 350
ILEPHVFPGN CWAFEGDQGQ VVIQLPGRVQ LSDITLQHPP PSVEHTGGAN
360 370 380 390 400
SAPRDFAVFG LQVYDETEVS LGKFTFDVEK SEIQTFHLQN DPPAAFPKVK
410 420 430
IQILSNWGHP RFTCLYRVRA HGVRTSEGAE GSAQGPH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JJZ6 | C9JJZ6_HUMAN | Sperm-associated antigen 4 protein | SPAG4 | 214 | Annotation score: | ||
Q5JX49 | Q5JX49_HUMAN | Sperm-associated antigen 4 protein | SPAG4 | 141 | Annotation score: |
Sequence cautioni
The sequence AAC32052 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF262992 mRNA Translation: AAF75267.1 AF262993 Genomic DNA Translation: AAF75268.1 AL109827 Genomic DNA No translation available. AF043344 Genomic DNA Translation: AAC32052.1 Sequence problems. |
CCDSi | CCDS13259.1 |
RefSeqi | NP_003107.1, NM_003116.2 |
Genome annotation databases
Ensembli | ENST00000374273; ENSP00000363391; ENSG00000061656 |
GeneIDi | 6676 |
KEGGi | hsa:6676 |
UCSCi | uc002xdb.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF262992 mRNA Translation: AAF75267.1 AF262993 Genomic DNA Translation: AAF75268.1 AL109827 Genomic DNA No translation available. AF043344 Genomic DNA Translation: AAC32052.1 Sequence problems. |
CCDSi | CCDS13259.1 |
RefSeqi | NP_003107.1, NM_003116.2 |
3D structure databases
SMRi | Q9NPE6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112558, 25 interactors |
IntActi | Q9NPE6, 27 interactors |
STRINGi | 9606.ENSP00000363391 |
PTM databases
iPTMneti | Q9NPE6 |
PhosphoSitePlusi | Q9NPE6 |
Polymorphism and mutation databases
BioMutai | SPAG4 |
DMDMi | 27805726 |
Proteomic databases
MassIVEi | Q9NPE6 |
PaxDbi | Q9NPE6 |
PeptideAtlasi | Q9NPE6 |
PRIDEi | Q9NPE6 |
ProteomicsDBi | 81980 |
Protocols and materials databases
Antibodypediai | 26200, 87 antibodies |
DNASUi | 6676 |
Genome annotation databases
Ensembli | ENST00000374273; ENSP00000363391; ENSG00000061656 |
GeneIDi | 6676 |
KEGGi | hsa:6676 |
UCSCi | uc002xdb.2, human |
Organism-specific databases
CTDi | 6676 |
DisGeNETi | 6676 |
EuPathDBi | HostDB:ENSG00000061656.9 |
GeneCardsi | SPAG4 |
HGNCi | HGNC:11214, SPAG4 |
HPAi | ENSG00000061656, Tissue enhanced (pancreas) |
MIMi | 603038, gene |
neXtProti | NX_Q9NPE6 |
OpenTargetsi | ENSG00000061656 |
PharmGKBi | PA36050 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2687, Eukaryota |
GeneTreei | ENSGT00940000161566 |
HOGENOMi | CLU_043737_2_0_1 |
InParanoidi | Q9NPE6 |
OMAi | TNTAYFW |
OrthoDBi | 1569602at2759 |
PhylomeDBi | Q9NPE6 |
TreeFami | TF323915 |
Enzyme and pathway databases
PathwayCommonsi | Q9NPE6 |
Miscellaneous databases
BioGRID-ORCSi | 6676, 13 hits in 843 CRISPR screens |
ChiTaRSi | SPAG4, human |
GenomeRNAii | 6676 |
Pharosi | Q9NPE6, Tdark |
PROi | PR:Q9NPE6 |
RNActi | Q9NPE6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000061656, Expressed in pancreas and 147 other tissues |
ExpressionAtlasi | Q9NPE6, baseline and differential |
Genevisiblei | Q9NPE6, HS |
Family and domain databases
Gene3Di | 2.60.120.260, 1 hit |
InterProi | View protein in InterPro IPR008979, Galactose-bd-like_sf IPR027776, SPAG4/SUN4 IPR012919, SUN_dom |
PANTHERi | PTHR12911:SF16, PTHR12911:SF16, 1 hit |
Pfami | View protein in Pfam PF07738, Sad1_UNC, 1 hit |
PROSITEi | View protein in PROSITE PS51469, SUN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPAG4_HUMAN | |
Accessioni | Q9NPE6Primary (citable) accession number: Q9NPE6 Secondary accession number(s): O43648 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 17, 2003 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human chromosome 20
Human chromosome 20: entries, gene names and cross-references to MIM