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Protein

Ubiquitin-conjugating enzyme E2 T

Gene

UBE2T

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed:20061386).7 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-6783310 Fanconi Anemia Pathway
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
SignaLinkiQ9NPD8
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 T (EC:2.3.2.231 Publication)
Alternative name(s):
Cell proliferation-inducing gene 50 protein
E2 ubiquitin-conjugating enzyme T
Ubiquitin carrier protein T
Ubiquitin-protein ligase T
Gene namesi
Name:UBE2T
ORF Names:HSPC150, PIG50
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000077152.9
HGNCiHGNC:25009 UBE2T
MIMi610538 gene
neXtProtiNX_Q9NPD8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Fanconi anemia complementation group T (FANCT)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
See also OMIM:616435
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0738612Q → E in FANCT; abolishes FANCD2 monoubiquitination; abolishes interaction with FANCL. 1 PublicationCorresponds to variant dbSNP:rs774357609EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5S → R: No effect on FANCL-binding, nor on FANCL-dependent monoubiquitination of FANCD2. 1 Publication1
Mutagenesisi60R → E: Loss of FANCL-binding and of FANCL-dependent monoubiquitination of FANCD2. 1 Publication1
Mutagenesisi63F → A: Decreased binding to FANCL. 1 Publication1
Mutagenesisi73P → K: Decreased FANCD2 ubiquitination. 1 Publication1
Mutagenesisi86C → A: Loss of E2 enzyme activity. 5 Publications1
Mutagenesisi91K → R: Decreased monoubiquitination. 1 Publication1
Mutagenesisi99 – 101RPS → SPR: No effect on FANCL-binding, nor on FANCL-dependent monoubiquitination of FANCD2. 1 Publication3
Mutagenesisi182 – 191Missing : Decreased monoubiquitination. 1 Publication10

Keywords - Diseasei

Disease mutation, Fanconi anemia

Organism-specific databases

DisGeNETi29089
MalaCardsiUBE2T
MIMi616435 phenotype
OpenTargetsiENSG00000077152
Orphaneti84 Fanconi anemia
PharmGKBiPA142670655

Polymorphism and mutation databases

BioMutaiUBE2T
DMDMi73622065

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825091 – 197Ubiquitin-conjugating enzyme E2 TAdd BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki91Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei184PhosphoserineCombined sources1
Cross-linki191Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki192Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Auto-ubiquitinated. Effects of auto-monoubiquitination at Lys-91 and Lys-182 are unclear: according to a report, monoubiquitination inactivates E2 enzyme activity (PubMed:16916645). In contrast, according to another report, autoubiquitination does not affect E2 enzyme activity (PubMed:19111657).4 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9NPD8
MaxQBiQ9NPD8
PaxDbiQ9NPD8
PeptideAtlasiQ9NPD8
PRIDEiQ9NPD8
ProteomicsDBi81976
TopDownProteomicsiQ9NPD8

PTM databases

iPTMnetiQ9NPD8
PhosphoSitePlusiQ9NPD8
SwissPalmiQ9NPD8

Expressioni

Inductioni

Down-regulated following hypoxia. Up-regulated in breast cancers.1 Publication

Gene expression databases

BgeeiENSG00000077152 Expressed in 167 organ(s), highest expression level in oocyte
CleanExiHS_UBE2T
ExpressionAtlasiQ9NPD8 baseline and differential
GenevisibleiQ9NPD8 HS

Organism-specific databases

HPAiHPA002831

Interactioni

Subunit structurei

Directly interacts with FANCL (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:21775430, PubMed:24389026). Interacts with BRCA1 (PubMed:19887602).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FANCLQ9NW38-13EBI-2130165,EBI-16088720

GO - Molecular functioni

Protein-protein interaction databases

BioGridi118858, 45 interactors
DIPiDIP-52740N
IntActiQ9NPD8, 13 interactors
MINTiQ9NPD8
STRINGi9606.ENSP00000356243

Structurei

Secondary structure

1197
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9NPD8
SMRiQ9NPD8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9NPD8

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00540000070023
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiQ9NPD8
KOiK13960
OMAiIEKKFCP
OrthoDBiEOG091G0VPD
PhylomeDBiQ9NPD8
TreeFamiTF354203

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9NPD8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQRASRLKRE LHMLATEPPP GITCWQDKDQ MDDLRAQILG GANTPYEKGV
60 70 80 90 100
FKLEVIIPER YPFEPPQIRF LTPIYHPNID SAGRICLDVL KLPPKGAWRP
110 120 130 140 150
SLNIATVLTS IQLLMSEPNP DDPLMADISS EFKYNKPAFL KNARQWTEKH
160 170 180 190
ARQKQKADEE EMLDNLPEAG DSRVHNSTQK RKASQLVGIE KKFHPDV
Length:197
Mass (Da):22,521
Last modified:October 1, 2000 - v1
Checksum:i6C02D774A7FA928A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8Y812A0A2R8Y812_HUMAN
Ubiquitin-conjugating enzyme E2 T
UBE2T
47Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0738612Q → E in FANCT; abolishes FANCD2 monoubiquitination; abolishes interaction with FANCL. 1 PublicationCorresponds to variant dbSNP:rs774357609EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032931 mRNA Translation: BAA93711.1
AF160215 mRNA Translation: AAF67016.1
AF161499 mRNA Translation: AAF29114.1
AK000504 mRNA Translation: BAA91211.1
AY542309 mRNA Translation: AAT08178.1
AL356953 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW91411.1
BC004152 mRNA Translation: AAH04152.1
BC019284 mRNA Translation: AAH19284.1
CCDSiCCDS1425.1
RefSeqiNP_001297255.1, NM_001310326.1
NP_054895.1, NM_014176.3
UniGeneiHs.5199

Genome annotation databases

EnsembliENST00000367274; ENSP00000356243; ENSG00000077152
ENST00000646651; ENSP00000494957; ENSG00000077152
GeneIDi29089
KEGGihsa:29089
UCSCiuc001gxx.5 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB032931 mRNA Translation: BAA93711.1
AF160215 mRNA Translation: AAF67016.1
AF161499 mRNA Translation: AAF29114.1
AK000504 mRNA Translation: BAA91211.1
AY542309 mRNA Translation: AAT08178.1
AL356953 Genomic DNA No translation available.
CH471067 Genomic DNA Translation: EAW91411.1
BC004152 mRNA Translation: AAH04152.1
BC019284 mRNA Translation: AAH19284.1
CCDSiCCDS1425.1
RefSeqiNP_001297255.1, NM_001310326.1
NP_054895.1, NM_014176.3
UniGeneiHs.5199

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YH2X-ray2.00A1-167[»]
4CCGX-ray2.40A/B1-197[»]
5NGZX-ray2.40A1-197[»]
5OJJX-ray1.85A/B/C/D/E/F1-154[»]
ProteinModelPortaliQ9NPD8
SMRiQ9NPD8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118858, 45 interactors
DIPiDIP-52740N
IntActiQ9NPD8, 13 interactors
MINTiQ9NPD8
STRINGi9606.ENSP00000356243

PTM databases

iPTMnetiQ9NPD8
PhosphoSitePlusiQ9NPD8
SwissPalmiQ9NPD8

Polymorphism and mutation databases

BioMutaiUBE2T
DMDMi73622065

Proteomic databases

EPDiQ9NPD8
MaxQBiQ9NPD8
PaxDbiQ9NPD8
PeptideAtlasiQ9NPD8
PRIDEiQ9NPD8
ProteomicsDBi81976
TopDownProteomicsiQ9NPD8

Protocols and materials databases

DNASUi29089
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367274; ENSP00000356243; ENSG00000077152
ENST00000646651; ENSP00000494957; ENSG00000077152
GeneIDi29089
KEGGihsa:29089
UCSCiuc001gxx.5 human

Organism-specific databases

CTDi29089
DisGeNETi29089
EuPathDBiHostDB:ENSG00000077152.9
GeneCardsiUBE2T
HGNCiHGNC:25009 UBE2T
HPAiHPA002831
MalaCardsiUBE2T
MIMi610538 gene
616435 phenotype
neXtProtiNX_Q9NPD8
OpenTargetsiENSG00000077152
Orphaneti84 Fanconi anemia
PharmGKBiPA142670655
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0417 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00540000070023
HOGENOMiHOG000233455
HOVERGENiHBG063308
InParanoidiQ9NPD8
KOiK13960
OMAiIEKKFCP
OrthoDBiEOG091G0VPD
PhylomeDBiQ9NPD8
TreeFamiTF354203

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B6 2681
ReactomeiR-HSA-6783310 Fanconi Anemia Pathway
R-HSA-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
SignaLinkiQ9NPD8

Miscellaneous databases

ChiTaRSiUBE2T human
EvolutionaryTraceiQ9NPD8
GenomeRNAii29089
PROiPR:Q9NPD8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000077152 Expressed in 167 organ(s), highest expression level in oocyte
CleanExiHS_UBE2T
ExpressionAtlasiQ9NPD8 baseline and differential
GenevisibleiQ9NPD8 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2T_HUMAN
AccessioniPrimary (citable) accession number: Q9NPD8
Secondary accession number(s): Q2TU36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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