Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Matrix metalloproteinase-25

Gene

MMP25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May activate progelatinase A.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi90Zinc; in inhibited formBy similarity1
Metal bindingi233Zinc; catalyticPROSITE-ProRule annotation1
Active sitei234PROSITE-ProRule annotation1
Metal bindingi237Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi243Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • hard palate development Source: Ensembl
  • inflammatory response Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1592389 Activation of Matrix Metalloproteinases
R-HSA-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiM10.024

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-25 (EC:3.4.24.-)
Short name:
MMP-25
Alternative name(s):
Leukolysin
Membrane-type matrix metalloproteinase 6
Short name:
MT-MMP 6
Short name:
MTMMP6
Membrane-type-6 matrix metalloproteinase
Short name:
MT6-MMP
Short name:
MT6MMP
Gene namesi
Name:MMP25
Synonyms:MMP20, MMPL1, MT6MMP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000008516.16
HGNCiHGNC:14246 MMP25
MIMi608482 gene
neXtProtiNX_Q9NPA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi64386
OpenTargetsiENSG00000008516
PharmGKBiPA30882

Chemistry databases

ChEMBLiCHEMBL1795103
DrugBankiDB00786 Marimastat
GuidetoPHARMACOLOGYi1647

Polymorphism and mutation databases

BioMutaiMMP25
DMDMi12585274

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000002885222 – 107By similarityAdd BLAST86
ChainiPRO_0000028853108 – 539Matrix metalloproteinase-25Add BLAST432
PropeptideiPRO_0000028854540 – 562Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi317 ↔ 508By similarity
Lipidationi539GPI-anchor amidated alanineSequence analysis1

Post-translational modificationi

The precursor is cleaved by a furin endopeptidase.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

EPDiQ9NPA2
PaxDbiQ9NPA2
PeptideAtlasiQ9NPA2
PRIDEiQ9NPA2
ProteomicsDBi81949

PTM databases

iPTMnetiQ9NPA2
PhosphoSitePlusiQ9NPA2

Expressioni

Tissue specificityi

Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas.

Gene expression databases

BgeeiENSG00000008516
CleanExiHS_MMP20
HS_MMP25
ExpressionAtlasiQ9NPA2 baseline and differential
GenevisibleiQ9NPA2 HS

Organism-specific databases

HPAiCAB025177

Interactioni

Protein-protein interaction databases

BioGridi122149, 1 interactor
IntActiQ9NPA2, 1 interactor
STRINGi9606.ENSP00000337816

Chemistry databases

BindingDBiQ9NPA2

Structurei

3D structure databases

ProteinModelPortaliQ9NPA2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati314 – 363Hemopexin 1Add BLAST50
Repeati367 – 412Hemopexin 2Add BLAST46
Repeati413 – 461Hemopexin 3Add BLAST49
Repeati462 – 508Hemopexin 4Add BLAST47

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi88 – 95Cysteine switchBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi103 – 107Poly-Arg5
Compositional biasi549 – 555Poly-Leu7

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1565 Eukaryota
ENOG410XQ5D LUCA
GeneTreeiENSGT00760000118870
HOGENOMiHOG000217928
HOVERGENiHBG052484
InParanoidiQ9NPA2
KOiK08003
OMAiVDWLTRY
OrthoDBiEOG091G03DP
PhylomeDBiQ9NPA2
TreeFamiTF315428

Family and domain databases

CDDicd00094 HX, 1 hit
cd04278 ZnMc_MMP, 1 hit
Gene3Di2.110.10.10, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR000585 Hemopexin-like_dom
IPR036375 Hemopexin-like_dom_sf
IPR018487 Hemopexin-like_repeat
IPR033739 M10A_MMP
IPR024079 MetalloPept_cat_dom_sf
IPR028733 MMP25
IPR001818 Pept_M10_metallopeptidase
IPR021190 Pept_M10A
IPR006026 Peptidase_Metallo
IPR002477 Peptidoglycan-bd-like
PANTHERiPTHR10201:SF142 PTHR10201:SF142, 1 hit
PfamiView protein in Pfam
PF00045 Hemopexin, 4 hits
PF00413 Peptidase_M10, 1 hit
PF01471 PG_binding_1, 1 hit
PIRSFiPIRSF001191 Peptidase_M10A_matrix, 1 hit
PRINTSiPR00138 MATRIXIN
SMARTiView protein in SMART
SM00120 HX, 4 hits
SM00235 ZnMc, 1 hit
SUPFAMiSSF50923 SSF50923, 1 hit
PROSITEiView protein in PROSITE
PS51642 HEMOPEXIN_2, 4 hits
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9NPA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLRLRLLAL LLLLLAPPAR APKPSAQDVS LGVDWLTRYG YLPPPHPAQA
60 70 80 90 100
QLQSPEKLRD AIKVMQRFAG LPETGRMDPG TVATMRKPRC SLPDVLGVAG
110 120 130 140 150
LVRRRRRYAL SGSVWKKRTL TWRVRSFPQS SQLSQETVRV LMSYALMAWG
160 170 180 190 200
MESGLTFHEV DSPQGQEPDI LIDFARAFHQ DSYPFDGLGG TLAHAFFPGE
210 220 230 240 250
HPISGDTHFD DEETWTFGSK DGEGTDLFAV AVHEFGHALG LGHSSAPNSI
260 270 280 290 300
MRPFYQGPVG DPDKYRLSQD DRDGLQQLYG KAPQTPYDKP TRKPLAPPPQ
310 320 330 340 350
PPASPTHSPS FPIPDRCEGN FDAIANIRGE TFFFKGPWFW RLQPSGQLVS
360 370 380 390 400
PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG
410 420 430 440 450
GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY
460 470 480 490 500
PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP
510 520 530 540 550
MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQAA GRWPAPIPLL
560
LLPLLVGGVA SR
Length:562
Mass (Da):62,554
Last modified:October 1, 2000 - v1
Checksum:iA6A50AE05D969C64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47P → R in BAB20584 (PubMed:11034316).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ239053 mRNA Translation: CAB94713.1
AF145442 mRNA Translation: AAF66697.2
AF185270 mRNA Translation: AAG17007.1
AB042328 mRNA Translation: BAB20584.1
AJ272137 mRNA Translation: CAC03490.1
CH471112 Genomic DNA Translation: EAW85413.1
CH471112 Genomic DNA Translation: EAW85415.1
CCDSiCCDS10492.1
RefSeqiNP_071913.1, NM_022468.4
UniGeneiHs.654979

Genome annotation databases

EnsembliENST00000336577; ENSP00000337816; ENSG00000008516
GeneIDi64386
KEGGihsa:64386
UCSCiuc002cth.4 human

Similar proteinsi

Entry informationi

Entry nameiMMP25_HUMAN
AccessioniPrimary (citable) accession number: Q9NPA2
Secondary accession number(s): D3DUA8, Q9H3Q0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: October 1, 2000
Last modified: June 20, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health