UniProtKB - Q9NPA2 (MMP25_HUMAN)
Protein
Matrix metalloproteinase-25
Gene
MMP25
Organism
Homo sapiens (Human)
Status
Functioni
May activate progelatinase A.
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 90 | Zinc; in inhibited formBy similarity | 1 | |
Metal bindingi | 233 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 234 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 237 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 243 | Zinc; catalyticPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- metalloendopeptidase activity Source: GO_Central
- zinc ion binding Source: InterPro
GO - Biological processi
- collagen catabolic process Source: GO_Central
- extracellular matrix organization Source: GO_Central
- hard palate development Source: Ensembl
- inflammatory response Source: UniProtKB
- neutrophil degranulation Source: Reactome
- proteolysis Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q9NPA2 |
Reactomei | R-HSA-1592389, Activation of Matrix Metalloproteinases R-HSA-6798695, Neutrophil degranulation |
SIGNORi | Q9NPA2 |
Protein family/group databases
MEROPSi | M10.024 |
Names & Taxonomyi
Protein namesi | Recommended name: Matrix metalloproteinase-25 (EC:3.4.24.-)Short name: MMP-25 Alternative name(s): Leukolysin Membrane-type matrix metalloproteinase 6 Short name: MT-MMP 6 Short name: MTMMP6 Membrane-type-6 matrix metalloproteinase Short name: MT6-MMP Short name: MT6MMP |
Gene namesi | Name:MMP25 Synonyms:MMP20, MMPL1, MT6MMP |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000008516.16 |
HGNCi | HGNC:14246, MMP25 |
MIMi | 608482, gene |
neXtProti | NX_Q9NPA2 |
Subcellular locationi
Plasma membrane
Extracellular region or secreted
Extracellular region or secreted
- extracellular matrix Source: UniProtKB
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- anchored component of membrane Source: UniProtKB-KW
- integral component of membrane Source: InterPro
- membrane Source: UniProtKB
- specific granule membrane Source: Reactome
Keywords - Cellular componenti
Cell membrane, Extracellular matrix, Membrane, SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 64386 |
OpenTargetsi | ENSG00000008516 |
PharmGKBi | PA30882 |
Miscellaneous databases
Pharosi | Q9NPA2, Tchem |
Chemistry databases
ChEMBLi | CHEMBL1795103 |
DrugBanki | DB01039, Fenofibrate DB13873, Fenofibric acid DB00786, Marimastat |
GuidetoPHARMACOLOGYi | 1647 |
Polymorphism and mutation databases
BioMutai | MMP25 |
DMDMi | 12585274 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000028852 | 22 – 107 | By similarityAdd BLAST | 86 | |
ChainiPRO_0000028853 | 108 – 539 | Matrix metalloproteinase-25Add BLAST | 432 | |
PropeptideiPRO_0000028854 | 540 – 562 | Removed in mature formSequence analysisAdd BLAST | 23 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 317 ↔ 508 | By similarity | ||
Lipidationi | 539 | GPI-anchor amidated alanineSequence analysis | 1 |
Post-translational modificationi
The precursor is cleaved by a furin endopeptidase.By similarity
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, ZymogenProteomic databases
EPDi | Q9NPA2 |
MassIVEi | Q9NPA2 |
PaxDbi | Q9NPA2 |
PeptideAtlasi | Q9NPA2 |
PRIDEi | Q9NPA2 |
ProteomicsDBi | 81949 |
PTM databases
GlyGeni | Q9NPA2, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NPA2 |
PhosphoSitePlusi | Q9NPA2 |
Expressioni
Tissue specificityi
Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas.
Gene expression databases
Bgeei | ENSG00000008516, Expressed in blood and 218 other tissues |
ExpressionAtlasi | Q9NPA2, baseline and differential |
Genevisiblei | Q9NPA2, HS |
Organism-specific databases
HPAi | ENSG00000008516, Tissue enhanced (blood, bone marrow, lymphoid tissue) |
Interactioni
Binary interactionsi
Q9NPA2
With | #Exp. | IntAct |
---|---|---|
MEOX2 [Q6FHY5] | 3 | EBI-12346397,EBI-16439278 |
Protein-protein interaction databases
BioGRIDi | 122149, 8 interactors |
IntActi | Q9NPA2, 2 interactors |
STRINGi | 9606.ENSP00000337816 |
Chemistry databases
BindingDBi | Q9NPA2 |
Miscellaneous databases
RNActi | Q9NPA2, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 314 – 363 | Hemopexin 1Add BLAST | 50 | |
Repeati | 367 – 412 | Hemopexin 2Add BLAST | 46 | |
Repeati | 413 – 461 | Hemopexin 3Add BLAST | 49 | |
Repeati | 462 – 508 | Hemopexin 4Add BLAST | 47 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 88 – 95 | Cysteine switchBy similarity | 8 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 103 – 107 | Poly-Arg | 5 | |
Compositional biasi | 549 – 555 | Poly-Leu | 7 |
Domaini
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similaritiesi
Belongs to the peptidase M10A family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | KOG1565, Eukaryota |
GeneTreei | ENSGT00940000159799 |
HOGENOMi | CLU_015489_8_2_1 |
InParanoidi | Q9NPA2 |
OMAi | LWEGAPH |
OrthoDBi | 1075463at2759 |
PhylomeDBi | Q9NPA2 |
TreeFami | TF315428 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR028733, MMP25 IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like |
PANTHERi | PTHR10201:SF142, PTHR10201:SF142, 1 hit |
Pfami | View protein in Pfam PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9NPA2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLRLRLLAL LLLLLAPPAR APKPSAQDVS LGVDWLTRYG YLPPPHPAQA
60 70 80 90 100
QLQSPEKLRD AIKVMQRFAG LPETGRMDPG TVATMRKPRC SLPDVLGVAG
110 120 130 140 150
LVRRRRRYAL SGSVWKKRTL TWRVRSFPQS SQLSQETVRV LMSYALMAWG
160 170 180 190 200
MESGLTFHEV DSPQGQEPDI LIDFARAFHQ DSYPFDGLGG TLAHAFFPGE
210 220 230 240 250
HPISGDTHFD DEETWTFGSK DGEGTDLFAV AVHEFGHALG LGHSSAPNSI
260 270 280 290 300
MRPFYQGPVG DPDKYRLSQD DRDGLQQLYG KAPQTPYDKP TRKPLAPPPQ
310 320 330 340 350
PPASPTHSPS FPIPDRCEGN FDAIANIRGE TFFFKGPWFW RLQPSGQLVS
360 370 380 390 400
PRPARLHRFW EGLPAQVRVV QAAYARHRDG RILLFSGPQF WVFQDRQLEG
410 420 430 440 450
GARPLTELGL PPGEEVDAVF SWPQNGKTYL VRGRQYWRYD EAAARPDPGY
460 470 480 490 500
PRDLSLWEGA PPSPDDVTVS NAGDTYFFKG AHYWRFPKNS IKTEPDAPQP
510 520 530 540 550
MGPNWLDCPA PSSGPRAPRP PKATPVSETC DCQCELNQAA GRWPAPIPLL
560
LLPLLVGGVA SR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketO43923 | O43923_HUMAN | Matrix metalloproteinase-25 | MMP25 MMP20, mmp20 | 183 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | P → R in BAB20584 (PubMed:11034316).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ239053 mRNA Translation: CAB94713.1 AF145442 mRNA Translation: AAF66697.2 AF185270 mRNA Translation: AAG17007.1 AB042328 mRNA Translation: BAB20584.1 AJ272137 mRNA Translation: CAC03490.1 CH471112 Genomic DNA Translation: EAW85413.1 CH471112 Genomic DNA Translation: EAW85415.1 |
CCDSi | CCDS10492.1 |
RefSeqi | NP_071913.1, NM_022468.4 |
Genome annotation databases
Ensembli | ENST00000336577; ENSP00000337816; ENSG00000008516 |
GeneIDi | 64386 |
KEGGi | hsa:64386 |
UCSCi | uc002cth.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ239053 mRNA Translation: CAB94713.1 AF145442 mRNA Translation: AAF66697.2 AF185270 mRNA Translation: AAG17007.1 AB042328 mRNA Translation: BAB20584.1 AJ272137 mRNA Translation: CAC03490.1 CH471112 Genomic DNA Translation: EAW85413.1 CH471112 Genomic DNA Translation: EAW85415.1 |
CCDSi | CCDS10492.1 |
RefSeqi | NP_071913.1, NM_022468.4 |
3D structure databases
SMRi | Q9NPA2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 122149, 8 interactors |
IntActi | Q9NPA2, 2 interactors |
STRINGi | 9606.ENSP00000337816 |
Chemistry databases
BindingDBi | Q9NPA2 |
ChEMBLi | CHEMBL1795103 |
DrugBanki | DB01039, Fenofibrate DB13873, Fenofibric acid DB00786, Marimastat |
GuidetoPHARMACOLOGYi | 1647 |
Protein family/group databases
MEROPSi | M10.024 |
PTM databases
GlyGeni | Q9NPA2, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | Q9NPA2 |
PhosphoSitePlusi | Q9NPA2 |
Polymorphism and mutation databases
BioMutai | MMP25 |
DMDMi | 12585274 |
Proteomic databases
EPDi | Q9NPA2 |
MassIVEi | Q9NPA2 |
PaxDbi | Q9NPA2 |
PeptideAtlasi | Q9NPA2 |
PRIDEi | Q9NPA2 |
ProteomicsDBi | 81949 |
Protocols and materials databases
Antibodypediai | 10685, 298 antibodies |
DNASUi | 64386 |
Genome annotation databases
Ensembli | ENST00000336577; ENSP00000337816; ENSG00000008516 |
GeneIDi | 64386 |
KEGGi | hsa:64386 |
UCSCi | uc002cth.4, human |
Organism-specific databases
CTDi | 64386 |
DisGeNETi | 64386 |
EuPathDBi | HostDB:ENSG00000008516.16 |
GeneCardsi | MMP25 |
HGNCi | HGNC:14246, MMP25 |
HPAi | ENSG00000008516, Tissue enhanced (blood, bone marrow, lymphoid tissue) |
MIMi | 608482, gene |
neXtProti | NX_Q9NPA2 |
OpenTargetsi | ENSG00000008516 |
PharmGKBi | PA30882 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1565, Eukaryota |
GeneTreei | ENSGT00940000159799 |
HOGENOMi | CLU_015489_8_2_1 |
InParanoidi | Q9NPA2 |
OMAi | LWEGAPH |
OrthoDBi | 1075463at2759 |
PhylomeDBi | Q9NPA2 |
TreeFami | TF315428 |
Enzyme and pathway databases
PathwayCommonsi | Q9NPA2 |
Reactomei | R-HSA-1592389, Activation of Matrix Metalloproteinases R-HSA-6798695, Neutrophil degranulation |
SIGNORi | Q9NPA2 |
Miscellaneous databases
BioGRID-ORCSi | 64386, 11 hits in 842 CRISPR screens |
GeneWikii | MMP25 |
GenomeRNAii | 64386 |
Pharosi | Q9NPA2, Tchem |
PROi | PR:Q9NPA2 |
RNActi | Q9NPA2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000008516, Expressed in blood and 218 other tissues |
ExpressionAtlasi | Q9NPA2, baseline and differential |
Genevisiblei | Q9NPA2, HS |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR028733, MMP25 IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like |
PANTHERi | PTHR10201:SF142, PTHR10201:SF142, 1 hit |
Pfami | View protein in Pfam PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMP25_HUMAN | |
Accessioni | Q9NPA2Primary (citable) accession number: Q9NPA2 Secondary accession number(s): D3DUA8, Q9H3Q0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 189 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - Peptidase families
Classification of peptidase families and list of entries