SINHCAF

Functionⁱ

Subunit of the Sin3 deacetylase complex (Sin3/HDAC), this subunit is important for the repression of genes encoding components of the TGF-beta signaling pathway (PubMed:22865885, PubMed:22984288). Core component of a SIN3A complex (composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1) present in embryonic stem (ES) cells. Promotes the stability of SIN3A and its presence on chromatin and is essential for maintaining the potential of ES cells to proliferate rapidly, while ensuring a short G1-phase of the cell cycle, thereby preventing premature lineage priming (By similarity).By similarity2 Publications

GO - Biological processⁱ

negative regulation of cell differentiation Source: UniProtKB
negative regulation of cell migration
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 22984288
positive regulation of cell proliferation Source: UniProtKB

View the complete GO annotation on QuickGO ...

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SIN3-HDAC complex-associated factorImported Alternative name(s): Protein FAM60A Tera protein homolog
Gene namesⁱ	Name:SINHCAFImported Synonyms:C12orf14, FAM60A ORF Names:L4
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 12

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000139146.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:30702 SINHCAF
MIMⁱ	615027 gene
neXtProtⁱ	NX_Q9NP50

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	58516
Open Targets More...OpenTargetsⁱ	ENSG00000139146
PharmGKBⁱ	PA134921007

Polymorphism and mutation databases

BioMutaⁱ	FAM60A
DMDMⁱ	71151891

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000187086	1 – 221	SIN3-HDAC complex-associated factorAdd BLAST		221

Proteomic databases

EPDⁱ	Q9NP50
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9NP50
PaxDbⁱ	Q9NP50
PeptideAtlas More...PeptideAtlasⁱ	Q9NP50
PRIDEⁱ	Q9NP50
ProteomicsDBⁱ	81884 81885 [Q9NP50-2]

PTM databases

iPTMnetⁱ	Q9NP50
PhosphoSitePlusⁱ	Q9NP50

Expressionⁱ

Inductionⁱ

Peaks during G1 and S phases of the cell cycle in U2OS cells. Up-regulated in squamous cell carcinoma (SCC), adenocarcinoma (AC), colon, ovary, rectum and stomach tumors.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000139146 Expressed in 89 organ(s), highest expression level in kidney
CleanExⁱ	HS_FAM60A
ExpressionAtlasⁱ	Q9NP50 baseline and differential
Genevisibleⁱ	Q9NP50 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA038167 HPA057585

HPAⁱ

HPA038167
HPA057585

Interactionⁱ

Subunit structureⁱ

Interacts with the Sin3/HDAC corepressor complex at least composed of BRMS1, BRMS1L, ING2, SAP30, SAP30L and HDAC1 (PubMed:22865885, PubMed:22984288). Found in a complex composed of at least SINHCAF, SIN3A, HDAC1, SAP30, RBBP4, OGT and TET1. Interacts with SIN3A and OGT (By similarity).By similarity2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
HDAC1	Q13547	5	EBI-741906,EBI-301834

With

Entry

#Exp.

IntAct

Notes

HDAC1

Q13547

5

EBI-741906,EBI-301834

Protein-protein interaction databases

BioGridⁱ	121842, 60 interactors
ComplexPortalⁱ	CPX-3323 SIN3A histone deacetylase complex, ES cell-specific variant
IntActⁱ	Q9NP50, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q9NP50
STRINGⁱ	9606.ENSP00000337477

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9NP50
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the SINHCAF family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG410IKPK Eukaryota ENOG4111PRH LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000006485
HOVERGENⁱ	HBG053413
InParanoidⁱ	Q9NP50
OMAⁱ	DFQNCFG
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0YQC
PhylomeDBⁱ	Q9NP50
TreeFamⁱ	TF323911

Family and domain databases

InterProⁱ	View protein in InterPro IPR026065 FAM60A
PANTHERⁱ	PTHR13422 PTHR13422, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF15396 FAM60A, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: Q9NP50-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFGFHKPKMY RSIEGCCICR AKSSSSRFTD SKRYEKDFQS CFGLHETRSG 
        60         70         80         90        100
DICNACVLLV KRWKKLPAGS KKNWNHVVDA RAGPSLKTTL KPKKVKTLSG 
       110        120        130        140        150
NRIKSNQISK LQKEFKRHNS DAHSTTSSAS PAQSPCYSNQ SDDGSDTEMA 
       160        170        180        190        200
SGSNRTPVFS FLDLTYWKRQ KICCGIIYKG RFGEVLIDTH LFKPCCSNKK 
       210        220 
AAAEKPEEQG PEPLPISTQE W

Length:221

Mass (Da):24,852

Last modified:October 1, 2000 - v1

Checksum:ⁱF5905C8AAC76AD45

GO

Isoform 2 (identifier: Q9NP50-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-148: Missing.

« Hide

        10         20         30         40         50
MASGSNRTPV FSFLDLTYWK RQKICCGIIY KGRFGEVLID THLFKPCCSN 
        60         70 
KKAAAEKPEE QGPEPLPIST QEW

Show »

Length:73

Mass (Da):8,292

Checksum:ⁱ051702E1E409F11E

GO

Computationally mapped potential isoform sequencesⁱ

There are 3 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F5GZ82	F5GZ82_HUMAN	SIN3-HDAC complex-associated factor SIN3-HDAC complex-associated factor	SINHCAF	134	Annotation score:
F5H6U2	F5H6U2_HUMAN	SIN3-HDAC complex-associated factor SIN3-HDAC complex-associated factor	SINHCAF	126	Annotation score:
H0YGG0	H0YGG0_HUMAN	SIN3-HDAC complex-associated factor SIN3-HDAC complex-associated factor	SINHCAF	59	Annotation score:

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_049027	117	R → H. Corresponds to variant dbSNP:rs2304459Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_014705	1 – 148	Missing in isoform 2. 1 PublicationAdd BLAST		148

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY598323 mRNA Translation: AAT06734.1 AF087885 mRNA Translation: AAP97184.1 AF212220 mRNA Translation: AAF87322.1 AK026932 mRNA Translation: BAB15592.1 AL157432 mRNA Translation: CAB75656.1 AC024940 Genomic DNA No translation available. CH471116 Genomic DNA Translation: EAW88561.1 CH471116 Genomic DNA Translation: EAW88562.1 CH471116 Genomic DNA Translation: EAW88564.1 CH471116 Genomic DNA Translation: EAW88566.1 CH471116 Genomic DNA Translation: EAW88567.1 BC000024 mRNA Translation: AAH00024.1 BC004462 mRNA Translation: AAH04462.1 BC071966 mRNA Translation: AAH71966.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS8723.1 [Q9NP50-1]
PIRⁱ	T46918
NCBI Reference Sequences More...RefSeqⁱ	NP_001129283.1, NM_001135811.1 [Q9NP50-1] NP_001129284.1, NM_001135812.1 [Q9NP50-1] NP_067061.1, NM_021238.2 [Q9NP50-1] XP_016875247.1, XM_017019758.1 [Q9NP50-1] XP_016875248.1, XM_017019759.1 [Q9NP50-1] XP_016875249.1, XM_017019760.1 [Q9NP50-1] XP_016875250.1, XM_017019761.1 [Q9NP50-1] XP_016875251.1, XM_017019762.1 [Q9NP50-1]
UniGeneⁱ	Hs.505154

Genome annotation databases

Ensemblⁱ	ENST00000337682; ENSP00000337477; ENSG00000139146 [Q9NP50-1] ENST00000454658; ENSP00000393279; ENSG00000139146 [Q9NP50-1] ENST00000539409; ENSP00000443697; ENSG00000139146 [Q9NP50-2] ENST00000542983; ENSP00000439952; ENSG00000139146 [Q9NP50-2] ENST00000617411; ENSP00000479093; ENSG00000276371 [Q9NP50-1] ENST00000631598; ENSP00000488852; ENSG00000276371 [Q9NP50-2] ENST00000633702; ENSP00000488570; ENSG00000276371 [Q9NP50-1] ENST00000633815; ENSP00000488406; ENSG00000276371 [Q9NP50-2]
GeneIDⁱ	58516
KEGGⁱ	hsa:58516
UCSC genome browser More...UCSCⁱ	uc001rke.4 human [Q9NP50-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9NP50	SIN3-HDAC complex associated factor		CANLF		228	UniRef100_Q9NP50
Uncharacterized protein (Fragment)		DESRO		228
FAM60A isoform 3		PANTR		73
FAM60A isoform 3		PONAB		73
Isoform 2 of SIN3-HDAC complex-associated factor		HUMAN		73
+22

Protein	Similar proteins		Species	Score	Length	Source
Q9NP50	SIN3-HDAC complex associated factor		CANLF		228	UniRef90_Q9NP50
Uncharacterized protein (Fragment)		DESRO		228
FAM60A isoform 3		PANTR		73
FAM60A isoform 3		PONAB		73
Isoform 2 of SIN3-HDAC complex-associated factor		HUMAN		73
+35

Protein	Similar proteins		Species	Score	Length	Source
Q9NP50	SIN3-HDAC complex associated factor		CANLF		228	UniRef50_Q9NP50
Uncharacterized protein (Fragment)		DESRO		228
FAM60A isoform 3		PANTR		73
FAM60A isoform 3		PONAB		73
Isoform 2 of SIN3-HDAC complex-associated factor		HUMAN		73
+43

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY598323 mRNA Translation: AAT06734.1 AF087885 mRNA Translation: AAP97184.1 AF212220 mRNA Translation: AAF87322.1 AK026932 mRNA Translation: BAB15592.1 AL157432 mRNA Translation: CAB75656.1 AC024940 Genomic DNA No translation available. CH471116 Genomic DNA Translation: EAW88561.1 CH471116 Genomic DNA Translation: EAW88562.1 CH471116 Genomic DNA Translation: EAW88564.1 CH471116 Genomic DNA Translation: EAW88566.1 CH471116 Genomic DNA Translation: EAW88567.1 BC000024 mRNA Translation: AAH00024.1 BC004462 mRNA Translation: AAH04462.1 BC071966 mRNA Translation: AAH71966.1
CCDSⁱ	CCDS8723.1 [Q9NP50-1]
PIRⁱ	T46918
RefSeqⁱ	NP_001129283.1, NM_001135811.1 [Q9NP50-1] NP_001129284.1, NM_001135812.1 [Q9NP50-1] NP_067061.1, NM_021238.2 [Q9NP50-1] XP_016875247.1, XM_017019758.1 [Q9NP50-1] XP_016875248.1, XM_017019759.1 [Q9NP50-1] XP_016875249.1, XM_017019760.1 [Q9NP50-1] XP_016875250.1, XM_017019761.1 [Q9NP50-1] XP_016875251.1, XM_017019762.1 [Q9NP50-1]
UniGeneⁱ	Hs.505154

3D structure databases

ProteinModelPortalⁱ	Q9NP50
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	121842, 60 interactors
ComplexPortalⁱ	CPX-3323 SIN3A histone deacetylase complex, ES cell-specific variant
IntActⁱ	Q9NP50, 10 interactors
MINTⁱ	Q9NP50
STRINGⁱ	9606.ENSP00000337477

PTM databases

iPTMnetⁱ	Q9NP50
PhosphoSitePlusⁱ	Q9NP50

Polymorphism and mutation databases

BioMutaⁱ	FAM60A
DMDMⁱ	71151891

Proteomic databases

EPDⁱ	Q9NP50
MaxQBⁱ	Q9NP50
PaxDbⁱ	Q9NP50
PeptideAtlasⁱ	Q9NP50
PRIDEⁱ	Q9NP50
ProteomicsDBⁱ	81884 81885 [Q9NP50-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	58516
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000337682; ENSP00000337477; ENSG00000139146 [Q9NP50-1] ENST00000454658; ENSP00000393279; ENSG00000139146 [Q9NP50-1] ENST00000539409; ENSP00000443697; ENSG00000139146 [Q9NP50-2] ENST00000542983; ENSP00000439952; ENSG00000139146 [Q9NP50-2] ENST00000617411; ENSP00000479093; ENSG00000276371 [Q9NP50-1] ENST00000631598; ENSP00000488852; ENSG00000276371 [Q9NP50-2] ENST00000633702; ENSP00000488570; ENSG00000276371 [Q9NP50-1] ENST00000633815; ENSP00000488406; ENSG00000276371 [Q9NP50-2]
GeneIDⁱ	58516
KEGGⁱ	hsa:58516
UCSCⁱ	uc001rke.4 human [Q9NP50-1]

Organism-specific databases

CTDⁱ	58516
DisGeNETⁱ	58516
EuPathDBⁱ	HostDB:ENSG00000139146.13
GeneCardsⁱ	SINHCAF
HGNCⁱ	HGNC:30702 SINHCAF
HPAⁱ	HPA038167 HPA057585
MIMⁱ	615027 gene
neXtProtⁱ	NX_Q9NP50
OpenTargetsⁱ	ENSG00000139146
PharmGKBⁱ	PA134921007
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	ENOG410IKPK Eukaryota ENOG4111PRH LUCA
GeneTreeⁱ	ENSGT00390000006485
HOVERGENⁱ	HBG053413
InParanoidⁱ	Q9NP50
OMAⁱ	DFQNCFG
OrthoDBⁱ	EOG091G0YQC
PhylomeDBⁱ	Q9NP50
TreeFamⁱ	TF323911

Miscellaneous databases

ChiTaRSⁱ	FAM60A human
GeneWikiⁱ	FAM60A
GenomeRNAiⁱ	58516
Protein Ontology More...PROⁱ	PR:Q9NP50
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000139146 Expressed in 89 organ(s), highest expression level in kidney
CleanExⁱ	HS_FAM60A
ExpressionAtlasⁱ	Q9NP50 baseline and differential
Genevisibleⁱ	Q9NP50 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR026065 FAM60A
PANTHERⁱ	PTHR13422 PTHR13422, 1 hit
Pfamⁱ	View protein in Pfam PF15396 FAM60A, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SHCAF_HUMAN
Accessionⁱ	Q9NP50Primary (citable) accession number: Q9NP50 Secondary accession number(s): D3DUV8, Q9BSZ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM

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UniProtKB - Q9NP50 (SHCAF_HUMAN)

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SIN3-HDAC complex-associated factor

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

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Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ