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UniProtKB - Q9NJU9 (CDPK3_PLAF7)
Protein
Calcium-dependent protein kinase 3
Gene
CDPK3
Organism
Plasmodium falciparum (isolate 3D7)
Status
Functioni
Calcium-dependent protein kinase which acts as a sensor and effector of intracellular Ca2+ levels probably in part downstream of cGMP-activated PKG kinase (By similarity).
In the mosquito midgut, regulates the gliding motility of the ookinete which is essential for the ookinete to invade the midgut epithelium (By similarity).
However, another study showed that while required for ookinete invasion of the midgut epithelium, is not required for ookinete gliding motility (By similarity).
By similarityCatalytic activityi
Cofactori
Mg2+By similarity
Activity regulationi
Activated by calcium (Probable). Upon calcium binding to the EF-hand domain 2, the C-terminus of the junction domain (J domain) undergoes a conformational change which results in the dissociation of the pseudo-substrate inhibitory motif from the catalytic domain (Probable). This, in turn, may facilitate the autophosphorylation of the activation loop at Thr-279, which leads to the kinase activation (By similarity).By similarity2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 147 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 238 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 466 | Calcium 1; low affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 468 | Calcium 1; low affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 470 | Calcium 1; low affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 472 | Calcium 1; low affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 477 | Calcium 1; low affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 501 | Calcium 2; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 503 | Calcium 2; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 505 | Calcium 2; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 507 | Calcium 2; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 512 | Calcium 2; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 542 | Calcium 3; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 544 | Calcium 3; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 546 | Calcium 3; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 548 | Calcium 3; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 553 | Calcium 3; high affinityPROSITE-ProRule annotationCombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 124 – 132 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- calcium-dependent protein serine/threonine kinase activity Source: GO_Central
- calcium ion binding Source: InterPro
- calmodulin binding Source: GO_Central
- calmodulin-dependent protein kinase activity Source: GeneDB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: GO_Central
- protein autophosphorylation Source: GO_Central
- protein phosphorylation Source: GeneDB
Keywordsi
Molecular function | Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Differentiation |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-PFA-5687128, MAPK6/MAPK4 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium-dependent protein kinase 31 Publication (EC:2.7.11.1By similarity)Alternative name(s): PfCDPK31 Publication |
Gene namesi | Name:CDPK31 Publication Synonyms:CPK3 ORF Names:MAL3P3.17, PF3D7_0310100, PFC0420w |
Organismi | Plasmodium falciparum (isolate 3D7) |
Taxonomic identifieri | 36329 [NCBI] |
Taxonomic lineagei | Eukaryota › Sar › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodiidae › Plasmodium › Plasmodium (Laverania) › |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | PlasmoDB:PF3D7_0310100 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085839 | 1 – 562 | Calcium-dependent protein kinase 3Add BLAST | 562 |
Proteomic databases
PRIDEi | Q9NJU9 |
Expressioni
Developmental stagei
Expressed in sexual stages, but not in asexual stages.1 Publication
Interactioni
GO - Molecular functioni
- calmodulin binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 5833.PFC0420w |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q9NJU9 |
SMRi | Q9NJU9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9NJU9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 118 – 373 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 256 | |
Domaini | 418 – 453 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 453 – 487 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 35 | |
Domaini | 488 – 523 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 529 – 562 | EF-hand 4PROSITE-ProRule annotationAdd BLAST | 34 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 393 – 428 | J domainBy similarityAdd BLAST | 36 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 393 – 401 | J domain autoinhibitory motifBy similarity | 9 | |
Motifi | 402 – 411 | J domain EF-hand interaction motifBy similarity | 10 |
Domaini
The EF-hand domain 1 cannot bind calcium due to the presence of a Lys instead of an Asp at position 435 and a Gln instead of a Glu at position 442 preventing calcium binding (PubMed:28746405, PubMed:21287613). The EF-hand domains 3 and 4 probably bind calcium constitutively when calcium levels are low, while the EF-hand domain 2 binds calcium following an increase in calcium levels (PubMed:21287613).2 Publications
The junction domain (J domain) is composed of 2 motifs that maintain the kinase inactive. The N-terminal autoinhibitory motif acts as a pseudosubstrate inhibiting the catalytic domain while the C-terminal motif binds the EF-hand domains.By similarity
Sequence similaritiesi
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Keywords - Domaini
RepeatPhylogenomic databases
HOGENOMi | CLU_000288_37_4_1 |
InParanoidi | Q9NJU9 |
OMAi | CQSLGFC |
PhylomeDBi | Q9NJU9 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13499, EF-hand_7, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 4 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 3 hits PS50222, EF_HAND_2, 4 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9NJU9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNDLIIKNNK KGSCDVIIKY KCKKSDENIK RRKSSHKYIK NKSVVLGRSI
60 70 80 90 100
MTNKKEKLKG ALKYKGSKKE IKICNKKSMI KNDKDENTTL KSMKSDNFKF
110 120 130 140 150
SRRGFILSFT GNLEDFYNLS KEPLGKGTYG CVYKATDKLL KISRAVKVVS
160 170 180 190 200
KKKLKNIPRF RQEIDIMKNL DHPNVVKLLE TFEDSNQIYL VMELCTGGEL
210 220 230 240 250
FDKIVKKGCF VETFASFIMK QIFSVLNYLH IRNICHRDIK PENFLFYDMT
260 270 280 290 300
PESLIKIIDF GLASYFTHNN YEMKTKAGTP YYVAPQVLTG SYNYKCDMWS
310 320 330 340 350
SGVLFYILLC GYPPFFGESD HEILSMVKKG KYQFKGKEWN NISEEAKDLI
360 370 380 390 400
KRCLTMDADK RICASEALQH PWFKKKKYAF NMDMKMDIHV LENFKNYGLL
410 420 430 440 450
LKFQKLAMTI IAQQSNDYDV EKLKSTFLVL DEDGKGYITK EQLKKGLEKD
460 470 480 490 500
GLKLPYNFDL LLDQIDSDGS GKIDYTEFIA AALDRKQLSK KLIYCAFRVF
510 520 530 540 550
DVDNDGEITT AELAHILYNG NKKGNITQRD VNRVKRMIRD VDKNNDGKID
560
FHEFSEMMKL KF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF106064 Genomic DNA Translation: AAF63154.1 AL844502 Genomic DNA Translation: CAB11118.4 |
PIRi | T18445 |
RefSeqi | XP_001351174.1, XM_001351138.1 |
Genome annotation databases
EnsemblProtistsi | CAB11118; CAB11118; PF3D7_0310100 |
GeneIDi | 814416 |
KEGGi | pfa:PF3D7_0310100 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF106064 Genomic DNA Translation: AAF63154.1 AL844502 Genomic DNA Translation: CAB11118.4 |
PIRi | T18445 |
RefSeqi | XP_001351174.1, XM_001351138.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3K21 | X-ray | 1.15 | A | 388-560 | [»] | |
AlphaFoldDBi | Q9NJU9 | |||||
SMRi | Q9NJU9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 5833.PFC0420w |
Proteomic databases
PRIDEi | Q9NJU9 |
Genome annotation databases
EnsemblProtistsi | CAB11118; CAB11118; PF3D7_0310100 |
GeneIDi | 814416 |
KEGGi | pfa:PF3D7_0310100 |
Organism-specific databases
VEuPathDBi | PlasmoDB:PF3D7_0310100 |
Phylogenomic databases
HOGENOMi | CLU_000288_37_4_1 |
InParanoidi | Q9NJU9 |
OMAi | CQSLGFC |
PhylomeDBi | Q9NJU9 |
Enzyme and pathway databases
Reactomei | R-PFA-5687128, MAPK6/MAPK4 signaling |
Miscellaneous databases
EvolutionaryTracei | Q9NJU9 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13499, EF-hand_7, 2 hits PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 4 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 3 hits PS50222, EF_HAND_2, 4 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CDPK3_PLAF7 | |
Accessioni | Q9NJU9Primary (citable) accession number: Q9NJU9 Secondary accession number(s): O77333 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 5, 2004 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families