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Protein

Histone-lysine N-methyltransferase mes-4

Gene

mes-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Histone methyltransferase. Dimethylates 'Lys-36' of histone H3, a specific tag for epigenetic transcriptional activation. Plays a central role in early development and is responsible for all H3 'Lys-36' dimethylation until about the 40-cell stage. Indirectly involved in the global inactivation of the X chromosomes in germline cells, possibly by excluding the mes-2-mes-3-mes-6 repressive Polycomb complex from the autosomes (PubMed:12077420, PubMed:16968818). Not related to transcription elongation (PubMed:12077420, PubMed:16968818). Required for small-RNA-induced H3K27 trimethylation (PubMed:26365259).3 Publications

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri126 – 214PHD-type 1Add BLAST89
Zinc fingeri303 – 355PHD-type 2Add BLAST53

GO - Molecular functioni

  • histone methyltransferase activity Source: WormBase
  • histone methyltransferase activity (H3-K36 specific) Source: WormBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Methyltransferase, Transferase
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase mes-4 (EC:2.1.1.43)
Alternative name(s):
Maternal-effect sterile protein 4
Gene namesi
Name:mes-4
ORF Names:Y2H9A.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiY2H9A.1 ; CE27781 ; WBGene00003222 ; mes-4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Derepression of X-linked genes in the germline.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi877 – 898Missing in BN87; maternal-effect mutation. Progeny defects in gonad proliferation. Germ cell degeneration. Abolishes histone methyltransferase activity. 2 PublicationsAdd BLAST22

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860831 – 898Histone-lysine N-methyltransferase mes-4Add BLAST898

Proteomic databases

EPDiQ9NH52
PaxDbiQ9NH52
PeptideAtlasiQ9NH52

Expressioni

Tissue specificityi

In adults, it is predominantly expressed in the germline, and weakly expressed in intestinal cells.

Developmental stagei

Expressed both maternally and zygotically. Expressed in all cells of early embryos. In late embryos and L1 larva, it is weakly expressed in somatic cells, while it is expressed at intermediate levels in the primordial germ cells Z2 and Z3.1 Publication

Gene expression databases

BgeeiWBGene00003222 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Interactioni

Protein-protein interaction databases

BioGridi44841, 16 interactors
IntActiQ9NH52, 3 interactors
STRINGi6239.Y2H9A.1.2

Structurei

3D structure databases

ProteinModelPortaliQ9NH52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini537 – 665SETPROSITE-ProRule annotationAdd BLAST129
Domaini671 – 687Post-SETPROSITE-ProRule annotationAdd BLAST17

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri126 – 214PHD-type 1Add BLAST89
Zinc fingeri303 – 355PHD-type 2Add BLAST53

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1081 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00780000121845
InParanoidiQ9NH52
KOiK07117
OMAiWICPQHE
OrthoDBiEOG091G02U7
PhylomeDBiQ9NH52

Family and domain databases

InterProiView protein in InterPro
IPR003616 Post-SET_dom
IPR001214 SET_dom
PfamiView protein in Pfam
PF00856 SET, 1 hit
SMARTiView protein in SMART
SM00508 PostSET, 1 hit
SM00317 SET, 1 hit
PROSITEiView protein in PROSITE
PS50868 POST_SET, 1 hit
PS50280 SET, 1 hit

Sequencei

Sequence statusi: Complete.

Q9NH52-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLPSSGDSSK ENCAPQDGIV EKEGPNKPFK KKNVKQRNAT PQGAGSETSS
60 70 80 90 100
NAEDKQEEAP ILTNAPKDII SMQIQETKAF LSEYEEFVDT DYPKTTFTHV
110 120 130 140 150
PRKFLSVSPF TPIEADAEGE FLPDADSKCV ICHEEKDKTG KSDLITCHGY
160 170 180 190 200
INGEMDDGKR LLGNIPMFPC RSKFHASCMI NYNSGGFHFQ YAARLECQAR
210 220 230 240 250
LLCPLHCCNS CNLDHHKQSA YVGDIAECAL CLRAFHLTSC YPSGGRDLNV
260 270 280 290 300
SITIGGKVEK FEMIICPAHY LPGADVQFYN KHKKRKNAVT VVPKADVTMK
310 320 330 340 350
SHIKACCVIG CEKSSNSKTI MCKTCCRSFH SGCREVETLN GKPIPDDQCE
360 370 380 390 400
SCVCGDPIPQ NTLILAKWTD NSFWLALTLD WYKYPTGNRG NINFERLGYT
410 420 430 440 450
VVQWLIPQEN DKEKQPLMSI VPVSDIARLT KNYFSLAKNS TLRNLWEEKY
460 470 480 490 500
EEQADTALKR CPYVCKTVFG RLRTSVYYKC EPKLEEYHNN EVCNCEGADR
510 520 530 540 550
CTKLSCQYLA DDYECPPSCS KKGVCHNRQV SMGIVSEKIK LAATLCKGYG
560 570 580 590 600
VFAKGQIEKD EYICEYVGEI IDKAEKKRRL DSVSISRDFQ ANHYMMELHK
610 620 630 640 650
GLTVDAARYG NISRYINHSC DPNAASFVTK VFVKKTKEGS LYDTRSYIRA
660 670 680 690 700
IRTIDDGDEI TFSYNMNNEE NLPDCECGAE NCMGTMGKAK REKPEVADSS
710 720 730 740 750
EKAAKKNKSS KKKSVKNQNR KSQEAGKNGT ASKKSEISPS KPSTSSASST
760 770 780 790 800
SFVQQASWPI SQNKKNLKKN SNQPVADTGS TLSTSTELNF HEKPQELLSP
810 820 830 840 850
VSSRSRAASS STPRAQKSKS RRDDVESEAP PVKRATPSLQ TIQETGKAIE
860 870 880 890
FPATKSAITK ARALSTQSVP SPHTVEVKVR AISTRGRVQK ETKRFEPI
Length:898
Mass (Da):100,065
Last modified:October 1, 2000 - v1
Checksum:i0FA6BC754FC9E295
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233290 mRNA Translation: AAF62516.1
AL021448 Genomic DNA Translation: CAA16276.2
PIRiT26577
RefSeqiNP_506333.1, NM_073932.4
UniGeneiCel.6195

Genome annotation databases

EnsemblMetazoaiY2H9A.1; Y2H9A.1; WBGene00003222
GeneIDi179824
KEGGicel:CELE_Y2H9A.1
UCSCiY2H9A.1 c. elegans

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233290 mRNA Translation: AAF62516.1
AL021448 Genomic DNA Translation: CAA16276.2
PIRiT26577
RefSeqiNP_506333.1, NM_073932.4
UniGeneiCel.6195

3D structure databases

ProteinModelPortaliQ9NH52
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi44841, 16 interactors
IntActiQ9NH52, 3 interactors
STRINGi6239.Y2H9A.1.2

Proteomic databases

EPDiQ9NH52
PaxDbiQ9NH52
PeptideAtlasiQ9NH52

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiY2H9A.1; Y2H9A.1; WBGene00003222
GeneIDi179824
KEGGicel:CELE_Y2H9A.1
UCSCiY2H9A.1 c. elegans

Organism-specific databases

CTDi179824
WormBaseiY2H9A.1 ; CE27781 ; WBGene00003222 ; mes-4

Phylogenomic databases

eggNOGiKOG1081 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00780000121845
InParanoidiQ9NH52
KOiK07117
OMAiWICPQHE
OrthoDBiEOG091G02U7
PhylomeDBiQ9NH52

Miscellaneous databases

PROiPR:Q9NH52

Gene expression databases

BgeeiWBGene00003222 Expressed in 5 organ(s), highest expression level in multi-cellular organism

Family and domain databases

InterProiView protein in InterPro
IPR003616 Post-SET_dom
IPR001214 SET_dom
PfamiView protein in Pfam
PF00856 SET, 1 hit
SMARTiView protein in SMART
SM00508 PostSET, 1 hit
SM00317 SET, 1 hit
PROSITEiView protein in PROSITE
PS50868 POST_SET, 1 hit
PS50280 SET, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMES4_CAEEL
AccessioniPrimary (citable) accession number: Q9NH52
Secondary accession number(s): Q9XXS4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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Main funding by: National Institutes of Health

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