UniProtKB - Q9N179 (EPB41_BOVIN)
Protein 4.1
EPB41
Functioni
Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.
By similarityGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
- structural molecule activity Source: InterPro
GO - Biological processi
- actomyosin structure organization Source: GO_Central
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cortical actin cytoskeleton organization Source: InterPro
- positive regulation of protein localization to cell cortex Source: UniProtKB
- regulation of calcium ion transport Source: UniProtKB
- regulation of intestinal absorption Source: UniProtKB
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding |
Biological process | Cell cycle, Cell division, Mitosis, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Protein 4.1Short name: P4.1 Alternative name(s): 4.1R Band 4.1 Erythrocyte membrane protein band 4.1By similarity |
Gene namesi | |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000006667 |
VGNCi | VGNC:28519, EPB41 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Nucleus
- Nucleus By similarity
Other locations
- cell cortex By similarity
Cytoskeleton
- cytoskeleton Source: GO_Central
Nucleus
- nucleus Source: UniProtKB-SubCell
Plasma Membrane
- basolateral plasma membrane Source: UniProtKB
- plasma membrane Source: GO_Central
Other locations
- cell cortex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000219388 | 1 – 617 | Protein 4.1Add BLAST | 617 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 13 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 169 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 312 | PhosphoserineBy similarity | 1 | |
Modified residuei | 331 | PhosphoserineBy similarity | 1 | |
Modified residuei | 333 | PhosphoserineBy similarity | 1 | |
Modified residuei | 413 | Phosphotyrosine; by EGFRBy similarity | 1 | |
Modified residuei | 417 | PhosphoserineBy similarity | 1 | |
Modified residuei | 427 | PhosphoserineBy similarity | 1 | |
Modified residuei | 437 | PhosphoserineBy similarity | 1 | |
Modified residuei | 462 | PhosphoserineBy similarity | 1 | |
Modified residuei | 465 | Phosphoserine; by CDK1By similarity | 1 | |
Modified residuei | 489 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 612 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q9N179 |
Interactioni
Subunit structurei
Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin, CENPJ and DLG1. Also found to associate with contractile apparatus and tight junctions.
Interacts with NUMA1; this interaction is negatively regulated by CDK1 during metaphase and promotes anaphase-specific localization of NUMA1 in symmetrically dividing cells.
Interacts with ATP2B1; regulates small intestinal calcium absorption through regulation of membrane expression of ATP2B1 (By similarity).
By similarityGO - Molecular functioni
- actin binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000008765 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 282 | FERMPROSITE-ProRule annotationAdd BLAST | 282 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 308 – 401 | DisorderedSequence analysisAdd BLAST | 94 | |
Regioni | 401 – 466 | Spectrin--actin-bindingAdd BLAST | 66 | |
Regioni | 467 – 617 | C-terminal (CTD)Add BLAST | 151 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 376 – 401 | Basic and acidic residuesSequence analysisAdd BLAST | 26 |
Phylogenomic databases
eggNOGi | KOG3527, Eukaryota |
GeneTreei | ENSGT00940000157833 |
InParanoidi | Q9N179 |
OrthoDBi | 262540at2759 |
Family and domain databases
CDDi | cd14473, FERM_B-lobe, 1 hit |
Gene3Di | 1.20.80.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR008379, Band_4.1_C IPR019749, Band_41_domain IPR021187, Band_41_protein_chordates IPR000798, Ez/rad/moesin-like IPR014847, FERM-adjacent IPR014352, FERM/acyl-CoA-bd_prot_sf IPR035963, FERM_2 IPR019748, FERM_central IPR019747, FERM_CS IPR000299, FERM_domain IPR018979, FERM_N IPR018980, FERM_PH-like_C IPR011993, PH-like_dom_sf IPR007477, SAB_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR23280:SF12, PTHR23280:SF12, 2 hits |
Pfami | View protein in Pfam PF05902, 4_1_CTD, 1 hit PF08736, FA, 1 hit PF09380, FERM_C, 1 hit PF00373, FERM_M, 1 hit PF09379, FERM_N, 1 hit PF04382, SAB, 1 hit |
PRINTSi | PR00935, BAND41 PR00661, ERMFAMILY |
SMARTi | View protein in SMART SM00295, B41, 1 hit SM01195, FA, 1 hit SM01196, FERM_C, 1 hit |
SUPFAMi | SSF47031, SSF47031, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00660, FERM_1, 1 hit PS00661, FERM_2, 1 hit PS50057, FERM_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED YFGLAIWDNA
60 70 80 90 100
TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED ITRYYLCLQL
110 120 130 140 150
RQDIVSGRLP CSFATLALLG SYTIQSELGD YDPELHGADY VSDFKLAPNQ
160 170 180 190 200
TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD LHKAKDLEGV
210 220 230 240 250
DIILGVCSSG LLVYKEKLRI NRFPWPKVLK ISYKRSSFFI KIRPGEQEQY
260 270 280 290 300
ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK FLALGSKFRY
310 320 330 340 350
SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAV EPADRTPRPT
360 370 380 390 400
SAPAIAPSPA AEGGVPGAPV KKAQKETVQV EVKQEEAPPE DAEPEPSEAW
410 420 430 440 450
KKKRERLDGE NIYIRHSNLM LEDLDKSQEE IKKHHASISE LKKNFMESVP
460 470 480 490 500
EPRPSEWDKR LSTHSPFRTL NINGQIPTGE GPPLVKTQTV TISDTANAVK
510 520 530 540 550
SEIPTKDVPI VHTETKTITY EAAQTDDSNG DLDPGVLLTA QTITSETTSS
560 570 580 590 600
TTTTQITKTV KGGISETRIE KRIVITGDAD IDHDQVLVQA IKEAKEQHPD
610
MSVTKVVVHQ ETEISEE
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1LTG0 | A0A3Q1LTG0_BOVIN | Band 4.1 | EPB41 | 784 | Annotation score: | ||
A0A3Q1LQM4 | A0A3Q1LQM4_BOVIN | Band 4.1 | EPB41 | 741 | Annotation score: | ||
A0A3Q1LH48 | A0A3Q1LH48_BOVIN | Band 4.1 | EPB41 | 624 | Annotation score: | ||
A0A3Q1LYH7 | A0A3Q1LYH7_BOVIN | Band 4.1 | EPB41 | 636 | Annotation score: | ||
F1MCU4 | F1MCU4_BOVIN | Band 4.1 | EPB41 | 763 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF222767 mRNA Translation: AAF61703.1 |
RefSeqi | NP_776736.1, NM_174311.1 |
Genome annotation databases
Ensembli | ENSBTAT00000008760; ENSBTAP00000008760; ENSBTAG00000006667 |
GeneIDi | 281753 |
KEGGi | bta:281753 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF222767 mRNA Translation: AAF61703.1 |
RefSeqi | NP_776736.1, NM_174311.1 |
3D structure databases
BMRBi | Q9N179 |
SMRi | Q9N179 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000008765 |
Proteomic databases
PRIDEi | Q9N179 |
Genome annotation databases
Ensembli | ENSBTAT00000008760; ENSBTAP00000008760; ENSBTAG00000006667 |
GeneIDi | 281753 |
KEGGi | bta:281753 |
Organism-specific databases
CTDi | 2035 |
VEuPathDBi | HostDB:ENSBTAG00000006667 |
VGNCi | VGNC:28519, EPB41 |
Phylogenomic databases
eggNOGi | KOG3527, Eukaryota |
GeneTreei | ENSGT00940000157833 |
InParanoidi | Q9N179 |
OrthoDBi | 262540at2759 |
Gene expression databases
ExpressionAtlasi | Q9N179, baseline and differential |
Family and domain databases
CDDi | cd14473, FERM_B-lobe, 1 hit |
Gene3Di | 1.20.80.10, 1 hit 2.30.29.30, 1 hit |
InterProi | View protein in InterPro IPR008379, Band_4.1_C IPR019749, Band_41_domain IPR021187, Band_41_protein_chordates IPR000798, Ez/rad/moesin-like IPR014847, FERM-adjacent IPR014352, FERM/acyl-CoA-bd_prot_sf IPR035963, FERM_2 IPR019748, FERM_central IPR019747, FERM_CS IPR000299, FERM_domain IPR018979, FERM_N IPR018980, FERM_PH-like_C IPR011993, PH-like_dom_sf IPR007477, SAB_dom IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR23280:SF12, PTHR23280:SF12, 2 hits |
Pfami | View protein in Pfam PF05902, 4_1_CTD, 1 hit PF08736, FA, 1 hit PF09380, FERM_C, 1 hit PF00373, FERM_M, 1 hit PF09379, FERM_N, 1 hit PF04382, SAB, 1 hit |
PRINTSi | PR00935, BAND41 PR00661, ERMFAMILY |
SMARTi | View protein in SMART SM00295, B41, 1 hit SM01195, FA, 1 hit SM01196, FERM_C, 1 hit |
SUPFAMi | SSF47031, SSF47031, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS00660, FERM_1, 1 hit PS00661, FERM_2, 1 hit PS50057, FERM_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EPB41_BOVIN | |
Accessioni | Q9N179Primary (citable) accession number: Q9N179 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 5, 2001 |
Last sequence update: | October 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |