EPB41

Functionⁱ

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.By similarity

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW
calmodulin binding Source: UniProtKB-KW
structural molecule activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actomyosin structure organization
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cell cycle Source: UniProtKB-KW
cell division Source: UniProtKB-KW
cortical actin cytoskeleton organization Source: InterPro
positive regulation of protein localization to cell cortex Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Actin-binding, Calmodulin-binding
Biological process	Cell cycle, Cell division, Mitosis, Transport

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein 4.1 Short name: P4.1 Alternative name(s): 4.1R Band 4.1
Gene namesⁱ	Name:EPB41 Synonyms:E41P
Organismⁱ	Bos taurus (Bovine)
Taxonomic identifierⁱ	9913 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos
Proteomesⁱ	UP000009136 Componentⁱ: Unplaced

Keywords - Cellular componentⁱ

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000219388	1 – 617	Protein 4.1Add BLAST		617

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	13	PhosphotyrosineBy similarity	1
Modified residueⁱ	169	PhosphothreonineBy similarity	1
Modified residueⁱ	312	PhosphoserineBy similarity	1
Modified residueⁱ	331	PhosphoserineBy similarity	1
Modified residueⁱ	333	PhosphoserineBy similarity	1
Modified residueⁱ	413	Phosphotyrosine; by EGFRBy similarity	1
Modified residueⁱ	417	PhosphoserineBy similarity	1
Modified residueⁱ	427	PhosphoserineBy similarity	1
Modified residueⁱ	437	PhosphoserineBy similarity	1
Modified residueⁱ	462	PhosphoserineBy similarity	1
Modified residueⁱ	465	Phosphoserine; by CDK1By similarity	1
Modified residueⁱ	489	PhosphothreonineBy similarity	1
Modified residueⁱ	612	PhosphothreonineBy similarity	1

Post-translational modificationⁱ

Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.By similarity

Phosphorylation on Tyr-413 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.By similarity

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PRIDEⁱ	Q9N179

PRIDEⁱ

Q9N179

Interactionⁱ

Subunit structureⁱ

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin, CENPJ and DLG1. Also found to associate with contractile apparatus and tight junctions. Interacts with NUMA1; this interaction is negatively regulated by CDK1 during metaphase and promotes anaphase-specific localization of NUMA1 in symmetrically dividing cells.By similarity

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW
calmodulin binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9N179
SMRⁱ	Q9N179
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	1 – 282	FERMPROSITE-ProRule annotationAdd BLAST		282

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	401 – 466	Spectrin--actin-bindingAdd BLAST		66
Regionⁱ	467 – 617	C-terminal (CTD)Add BLAST		151

Phylogenomic databases

HOGENOMⁱ	HOG000228841
HOVERGENⁱ	HBG007777
InParanoidⁱ	Q9N179
KEGG Orthology (KO) More...KOⁱ	K06107

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd14473 FERM_B-lobe, 1 hit
Gene3Dⁱ	1.20.80.10, 1 hit 2.30.29.30, 1 hit
InterProⁱ	View protein in InterPro IPR008379 Band_4.1_C IPR019749 Band_41_domain IPR021187 Band_41_protein_chordates IPR000798 Ez/rad/moesin-like IPR014847 FERM-adjacent IPR014352 FERM/acyl-CoA-bd_prot_sf IPR035963 FERM_2 IPR019748 FERM_central IPR019747 FERM_CS IPR000299 FERM_domain IPR018979 FERM_N IPR018980 FERM_PH-like_C IPR011993 PH-like_dom_sf IPR007477 SAB_dom IPR029071 Ubiquitin-like_domsf
PANTHERⁱ	PTHR23280:SF12 PTHR23280:SF12, 2 hits
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF05902 4_1_CTD, 1 hit PF08736 FA, 1 hit PF09380 FERM_C, 1 hit PF00373 FERM_M, 1 hit PF09379 FERM_N, 1 hit PF04382 SAB, 1 hit
PRINTSⁱ	PR00935 BAND41 PR00661 ERMFAMILY
SMARTⁱ	View protein in SMART SM00295 B41, 1 hit SM01195 FA, 1 hit SM01196 FERM_C, 1 hit
SUPFAMⁱ	SSF47031 SSF47031, 1 hit SSF54236 SSF54236, 1 hit
PROSITEⁱ	View protein in PROSITE PS00660 FERM_1, 1 hit PS00661 FERM_2, 1 hit PS50057 FERM_3, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9N179-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED YFGLAIWDNA 
        60         70         80         90        100
TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED ITRYYLCLQL 
       110        120        130        140        150
RQDIVSGRLP CSFATLALLG SYTIQSELGD YDPELHGADY VSDFKLAPNQ 
       160        170        180        190        200
TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD LHKAKDLEGV 
       210        220        230        240        250
DIILGVCSSG LLVYKEKLRI NRFPWPKVLK ISYKRSSFFI KIRPGEQEQY 
       260        270        280        290        300
ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK FLALGSKFRY 
       310        320        330        340        350
SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAV EPADRTPRPT 
       360        370        380        390        400
SAPAIAPSPA AEGGVPGAPV KKAQKETVQV EVKQEEAPPE DAEPEPSEAW 
       410        420        430        440        450
KKKRERLDGE NIYIRHSNLM LEDLDKSQEE IKKHHASISE LKKNFMESVP 
       460        470        480        490        500
EPRPSEWDKR LSTHSPFRTL NINGQIPTGE GPPLVKTQTV TISDTANAVK 
       510        520        530        540        550
SEIPTKDVPI VHTETKTITY EAAQTDDSNG DLDPGVLLTA QTITSETTSS 
       560        570        580        590        600
TTTTQITKTV KGGISETRIE KRIVITGDAD IDHDQVLVQA IKEAKEQHPD 
       610 
MSVTKVVVHQ ETEISEE

Length:617

Mass (Da):69,258

Last modified:October 1, 2000 - v1

Checksum:ⁱ5B49D5008AD900FB

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF222767 mRNA Translation: AAF61703.1
NCBI Reference Sequences More...RefSeqⁱ	NP_776736.1, NM_174311.1
UniGeneⁱ	Bt.87209

Genome annotation databases

GeneIDⁱ	281753
KEGGⁱ	bta:281753

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9N179	Protein 4.1		BOVIN		525	UniRef100_Q9N179
UPI000D539242		BOVIN		827

Protein	Similar proteins		Species	Score	Length	Source
Q9N179	Protein 4.1		BOVIN		525	UniRef90_Q9N179
protein 4.1 isoform X4		DELLE		855
Erythrocyte membrane protein band 4.1		RAT		855
protein 4.1 isoform X6		Florida manatee		853
Erythrocyte membrane protein band 4.1		SAIBB		848
+118

Protein	Similar proteins		Species	Score	Length	Source
Q9N179	Protein 4.1	)	HUMAN		864	UniRef50_P11171
Protein 4.1		CANLF		810
protein 4.1 isoform X2		DELLE		891
protein 4.1 isoform X10		TURTR		891
protein 4.1 isoform X9		PHYCD		881
+1306

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF222767 mRNA Translation: AAF61703.1
RefSeqⁱ	NP_776736.1, NM_174311.1
UniGeneⁱ	Bt.87209

3D structure databases

ProteinModelPortalⁱ	Q9N179
SMRⁱ	Q9N179
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Proteomic databases

PRIDEⁱ	Q9N179

PRIDEⁱ

Q9N179

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	281753
KEGGⁱ	bta:281753

Organism-specific databases

CTDⁱ	2035

CTDⁱ

2035

Phylogenomic databases

HOGENOMⁱ	HOG000228841
HOVERGENⁱ	HBG007777
InParanoidⁱ	Q9N179
KOⁱ	K06107

Family and domain databases

CDDⁱ	cd14473 FERM_B-lobe, 1 hit
Gene3Dⁱ	1.20.80.10, 1 hit 2.30.29.30, 1 hit
InterProⁱ	View protein in InterPro IPR008379 Band_4.1_C IPR019749 Band_41_domain IPR021187 Band_41_protein_chordates IPR000798 Ez/rad/moesin-like IPR014847 FERM-adjacent IPR014352 FERM/acyl-CoA-bd_prot_sf IPR035963 FERM_2 IPR019748 FERM_central IPR019747 FERM_CS IPR000299 FERM_domain IPR018979 FERM_N IPR018980 FERM_PH-like_C IPR011993 PH-like_dom_sf IPR007477 SAB_dom IPR029071 Ubiquitin-like_domsf
PANTHERⁱ	PTHR23280:SF12 PTHR23280:SF12, 2 hits
Pfamⁱ	View protein in Pfam PF05902 4_1_CTD, 1 hit PF08736 FA, 1 hit PF09380 FERM_C, 1 hit PF00373 FERM_M, 1 hit PF09379 FERM_N, 1 hit PF04382 SAB, 1 hit
PRINTSⁱ	PR00935 BAND41 PR00661 ERMFAMILY
SMARTⁱ	View protein in SMART SM00295 B41, 1 hit SM01195 FA, 1 hit SM01196 FERM_C, 1 hit
SUPFAMⁱ	SSF47031 SSF47031, 1 hit SSF54236 SSF54236, 1 hit
PROSITEⁱ	View protein in PROSITE PS00660 FERM_1, 1 hit PS00661 FERM_2, 1 hit PS50057 FERM_3, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	41_BOVIN
Accessionⁱ	Q9N179Primary (citable) accession number: Q9N179
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 5, 2001
	Last sequence update:	October 1, 2000
	Last modified:	November 7, 2018
	This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9N179 (41_BOVIN)

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Protein 4.1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytoskeleton

Other locations

Cytoskeleton

Nucleus

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ