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Protein

Protein 4.1

Gene

EPB41

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding
Biological processCell cycle, Cell division, Mitosis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 4.1
Short name:
P4.1
Alternative name(s):
4.1R
Band 4.1
Gene namesi
Name:EPB41
Synonyms:E41P
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193881 – 617Protein 4.1Add BLAST617

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphotyrosineBy similarity1
Modified residuei169PhosphothreonineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei331PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei413Phosphotyrosine; by EGFRBy similarity1
Modified residuei417PhosphoserineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei462PhosphoserineBy similarity1
Modified residuei465Phosphoserine; by CDK1By similarity1
Modified residuei489PhosphothreonineBy similarity1
Modified residuei612PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated at multiple sites by different protein kinases and each phosphorylation event selectively modulates the protein's functions.By similarity
Phosphorylation on Tyr-413 reduces the ability of 4.1 to promote the assembly of the spectrin/actin/4.1 ternary complex.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9N179

Interactioni

Subunit structurei

Binds with a high affinity to glycophorin and with lower affinity to band III protein. Associates with the nuclear mitotic apparatus. Binds calmodulin, CENPJ and DLG1. Also found to associate with contractile apparatus and tight junctions. Interacts with NUMA1; this interaction is negatively regulated by CDK1 during metaphase and promotes anaphase-specific localization of NUMA1 in symmetrically dividing cells.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ9N179
SMRiQ9N179
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 282FERMPROSITE-ProRule annotationAdd BLAST282

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni401 – 466Spectrin--actin-bindingAdd BLAST66
Regioni467 – 617C-terminal (CTD)Add BLAST151

Phylogenomic databases

HOGENOMiHOG000228841
HOVERGENiHBG007777
InParanoidiQ9N179
KOiK06107

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR008379 Band_4.1_C
IPR019749 Band_41_domain
IPR021187 Band_41_protein_chordates
IPR000798 Ez/rad/moesin-like
IPR014847 FERM-adjacent
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR011993 PH-like_dom_sf
IPR007477 SAB_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR23280:SF12 PTHR23280:SF12, 2 hits
PfamiView protein in Pfam
PF05902 4_1_CTD, 1 hit
PF08736 FA, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PF04382 SAB, 1 hit
PRINTSiPR00935 BAND41
PR00661 ERMFAMILY
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01195 FA, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00660 FERM_1, 1 hit
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9N179-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHCKVSLLDD TVYECVVEKH AKGQDLLKRV CEHLNLLEED YFGLAIWDNA
60 70 80 90 100
TSKTWLDSAK EIKKQVRGVP WNFTFNVKFY PPDPAQLTED ITRYYLCLQL
110 120 130 140 150
RQDIVSGRLP CSFATLALLG SYTIQSELGD YDPELHGADY VSDFKLAPNQ
160 170 180 190 200
TKELEEKVME LHKSYRSMTP AQADLEFLEN AKKLSMYGVD LHKAKDLEGV
210 220 230 240 250
DIILGVCSSG LLVYKEKLRI NRFPWPKVLK ISYKRSSFFI KIRPGEQEQY
260 270 280 290 300
ESTIGFKLPS YRAAKKLWKV CVEHHTFFRL TSTDTIPKSK FLALGSKFRY
310 320 330 340 350
SGRTQAQTRQ ASALIDRPAP HFERTASKRA SRSLDGAAAV EPADRTPRPT
360 370 380 390 400
SAPAIAPSPA AEGGVPGAPV KKAQKETVQV EVKQEEAPPE DAEPEPSEAW
410 420 430 440 450
KKKRERLDGE NIYIRHSNLM LEDLDKSQEE IKKHHASISE LKKNFMESVP
460 470 480 490 500
EPRPSEWDKR LSTHSPFRTL NINGQIPTGE GPPLVKTQTV TISDTANAVK
510 520 530 540 550
SEIPTKDVPI VHTETKTITY EAAQTDDSNG DLDPGVLLTA QTITSETTSS
560 570 580 590 600
TTTTQITKTV KGGISETRIE KRIVITGDAD IDHDQVLVQA IKEAKEQHPD
610
MSVTKVVVHQ ETEISEE
Length:617
Mass (Da):69,258
Last modified:October 1, 2000 - v1
Checksum:i5B49D5008AD900FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222767 mRNA Translation: AAF61703.1
RefSeqiNP_776736.1, NM_174311.1
UniGeneiBt.87209

Genome annotation databases

GeneIDi281753
KEGGibta:281753

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222767 mRNA Translation: AAF61703.1
RefSeqiNP_776736.1, NM_174311.1
UniGeneiBt.87209

3D structure databases

ProteinModelPortaliQ9N179
SMRiQ9N179
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9N179

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281753
KEGGibta:281753

Organism-specific databases

CTDi2035

Phylogenomic databases

HOGENOMiHOG000228841
HOVERGENiHBG007777
InParanoidiQ9N179
KOiK06107

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di1.20.80.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR008379 Band_4.1_C
IPR019749 Band_41_domain
IPR021187 Band_41_protein_chordates
IPR000798 Ez/rad/moesin-like
IPR014847 FERM-adjacent
IPR014352 FERM/acyl-CoA-bd_prot_sf
IPR035963 FERM_2
IPR019748 FERM_central
IPR019747 FERM_CS
IPR000299 FERM_domain
IPR018979 FERM_N
IPR018980 FERM_PH-like_C
IPR011993 PH-like_dom_sf
IPR007477 SAB_dom
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR23280:SF12 PTHR23280:SF12, 2 hits
PfamiView protein in Pfam
PF05902 4_1_CTD, 1 hit
PF08736 FA, 1 hit
PF09380 FERM_C, 1 hit
PF00373 FERM_M, 1 hit
PF09379 FERM_N, 1 hit
PF04382 SAB, 1 hit
PRINTSiPR00935 BAND41
PR00661 ERMFAMILY
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM01195 FA, 1 hit
SM01196 FERM_C, 1 hit
SUPFAMiSSF47031 SSF47031, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS00660 FERM_1, 1 hit
PS00661 FERM_2, 1 hit
PS50057 FERM_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry namei41_BOVIN
AccessioniPrimary (citable) accession number: Q9N179
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: October 1, 2000
Last modified: November 7, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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