UniProtKB - Q9N0Z4 (AT11B_RABIT)
Phospholipid-transporting ATPase IF
ATP11B
Functioni
Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids, phosphatidylserines (PS) and phosphatidylethanolamines (PE), from the outer to the inner leaflet of intracellular membranes. May contribute to the maintenance of membrane lipid asymmetry in endosome compartment.
By similarityAppears to play a role in the subnuclear trafficking of transcription factors with RING motifs.
2 PublicationsCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + H+ + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).By similarity EC:7.6.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 399 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 813 | MagnesiumBy similarity | 1 | |
Metal bindingi | 817 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- magnesium ion binding Source: InterPro
- phosphatidylethanolamine flippase activity Source: RHEA
- phosphatidylserine floppase activity Source: RHEA
GO - Biological processi
- aminophospholipid transport Source: InterPro
Keywordsi
Molecular function | Translocase |
Biological process | Lipid transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Phospholipid-transporting ATPase IF (EC:7.6.2.1By similarity)Alternative name(s): ATPase IR ATPase class VI type 11B P4-ATPase flippase complex alpha subunit ATP11B RING finger-binding protein |
Gene namesi | Name:ATP11B |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Endosome
- Recycling endosome membrane By similarity; Multi-pass membrane protein Sequence analysis
- Early endosome By similarity
Golgi apparatus
- trans-Golgi network By similarity
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, mainly located in recycling endosomes.By similarity
Nucleus
- Nucleus inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Endosome
- early endosome Source: UniProtKB-SubCell
- recycling endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Nucleus
- nuclear inner membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | ‹1 – 47 | CytoplasmicSequence analysisAdd BLAST | ›47 | |
Transmembranei | 48 – 69 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 70 – 74 | ExtracellularSequence analysis | 5 | |
Transmembranei | 75 – 96 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 97 – 281 | CytoplasmicSequence analysisAdd BLAST | 185 | |
Transmembranei | 282 – 303 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 304 – 333 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 334 – 351 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 352 – 868 | CytoplasmicSequence analysisAdd BLAST | 517 | |
Transmembranei | 869 – 890 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 891 – 902 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 903 – 922 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 923 – 952 | CytoplasmicSequence analysisAdd BLAST | 30 | |
Transmembranei | 953 – 974 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 975 – 989 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 990 – 1012 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 1013 – 1017 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 1018 – 1039 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 1040 – 1057 | ExtracellularSequence analysisAdd BLAST | 18 | |
Transmembranei | 1058 – 1082 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 1083 – 1169 | CytoplasmicSequence analysisAdd BLAST | 87 |
Keywords - Cellular componenti
Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 799 | I → D: Increased binding to the RING-finger of HLTF. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046372 | ‹1 – 1169 | Phospholipid-transporting ATPase IFAdd BLAST | ›1169 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1146 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PRIDEi | Q9N0Z4 |
Expressioni
Tissue specificityi
Inductioni
Interactioni
Subunit structurei
Component of a P4-ATPase flippase complex which consists of a catalytic alpha subunit ATP11B and an accessory beta subunit TMEM30A.
By similarityProtein-protein interaction databases
STRINGi | 9986.ENSOCUP00000024785 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 794 – 802 | Required for binding to the RING-finger of HLTF | 9 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0206, Eukaryota |
InParanoidi | Q9N0Z4 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR030362, ATP11B IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092:SF57, PTHR24092:SF57, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
s (2)i Sequence
Sequence statusi: Fragment.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
LGFDPPHQSD TRTIYIANRF PQNGLYTPQK FIDNRIISSK YTVWNFVPKN
60 70 80 90 100
LFEQFRRVAN FYFLIIFLVQ LMIDTPTSPI TSGLPLFFVI TVTAIKQGYE
110 120 130 140 150
DWLRHNSDNE VNGAPVYVVR SGGLVKTRSK NIRVGDIVRI AKDEIFPADL
160 170 180 190 200
VLLSSDRLDG SCHVTTASLD GETNLKTHVA VPETAVLQTV ANLDTLVAVI
210 220 230 240 250
ECQQPEADLY RFMGRMIITQ QMEEIVRPLG PESLLLRGAR LKNTKEIFGV
260 270 280 290 300
AVYTGMETKM ALNYKSKSQK RSAVEKSMNT FLIIYLIILI SEAIISTILK
310 320 330 340 350
YTWQAEEKWD EPWYNQKTEH QRNSSKILRF ISDFLAFLVL YNFIIPISLY
360 370 380 390 400
VTVEMQKFLG SFFIGWDLDL YHEESDQKAQ VNTSDLNEEL GQVEYVFTDK
410 420 430 440 450
TGTLTENEMQ FRECSIHGMK YQEINGRLVP EGPTPDSSEG NLSYLSSLSH
460 470 480 490 500
VNSLSHLTSS SSFRTSPEND TELIKEHDLF FKAVSLCHTV QISSVQTDGI
510 520 530 540 550
GDGPWQSSLA PSQLEYYASS PDEKALVEAA ARIGIVFVGN TEETMEVKIL
560 570 580 590 600
GKLERYKLLH VLEFDSDRRR MSVIVQAPSG ERFLFAKGAE SSILPKCIGG
610 620 630 640 650
EIEKTRIHVD EFALKGLRTL CVAYRQFTSK EYEVIDRRLF EARTALQQRE
660 670 680 690 700
EKLADVFHYI EKDLILLGAT AVEDRLQDKV RETIEALRMA GIKVWVLTGD
710 720 730 740 750
KHETAVSVSL SCGHFHRTMN ILELTNQKSD SECAEQLRQL ARRITEDHVI
760 770 780 790 800
QHGLVVDGTS LSLALREHEK LFMEVCRNCS AVLCCRMAPL QKAKVIRLIK
810 820 830 840 850
ISPEKPITIG CWDGANDVSM IQEAHVGIGI MGKERRQAAR NSDYAIARFK
860 870 880 890 900
FLSKLLFVHG HFYYIRIATL VQYFFYKNVC FITPQFLYQF YCLFSQQTLY
910 920 930 940 950
DSVYLTLYNI CFTSLPILIY SLLEQHIDPH ILQNKPTLYR DISKNRLLSI
960 970 980 990 1000
KTFLYWTILG FSRSFIFLFG SYFLIGKDAS LLGNGQMFGN WTFGTLVFTV
1010 1020 1030 1040 1050
MVITVTVKMA LETHFWTWIN HLVTWGSIIF YFVFSLFYGG ILWPFLGSQN
1060 1070 1080 1090 1100
MYFVFIQLVS SGSAWFAIIL MVVTCLFLDV MKKVFDRQLH PTSTEKAQLT
1110 1120 1130 1140 1150
ETNSSIKCVD SLCCFPEGET TCTSVRRMLE RVIGRCSPTH ISRSWSASDP
1160
FYTNDRSILT LSTMDSSTC
The sequence of this isoform differs from the canonical sequence as follows:
327-392: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Sequence conflicti | 133 | R → RGMHL in AAF68024 (PubMed:11058586).Curated | 1 | |
Sequence conflicti | 217 | I → V in AAF68024 (PubMed:11058586).Curated | 1 | |
Sequence conflicti | 283 | I → T in AAF68024 (PubMed:11058586).Curated | 1 | |
Sequence conflicti | 299 | L → S in AAL57758 (PubMed:11790799).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007308 | 327 – 392 | Missing in isoform 2. 1 PublicationAdd BLAST | 66 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF236061 mRNA Translation: AAF68024.1 AY069938 mRNA Translation: AAL57758.1 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF236061 mRNA Translation: AAF68024.1 AY069938 mRNA Translation: AAL57758.1 |
3D structure databases
AlphaFoldDBi | Q9N0Z4 |
SMRi | Q9N0Z4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000024785 |
Proteomic databases
PRIDEi | Q9N0Z4 |
Phylogenomic databases
eggNOGi | KOG0206, Eukaryota |
InParanoidi | Q9N0Z4 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR030362, ATP11B IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006539, P-type_ATPase_IV IPR032631, P-type_ATPase_N IPR001757, P_typ_ATPase IPR032630, P_typ_ATPase_c IPR044492, P_typ_ATPase_HD_dom |
PANTHERi | PTHR24092:SF57, PTHR24092:SF57, 1 hit |
Pfami | View protein in Pfam PF16212, PhoLip_ATPase_C, 1 hit PF16209, PhoLip_ATPase_N, 1 hit |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01652, ATPase-Plipid, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT11B_RABIT | |
Accessioni | Q9N0Z4Primary (citable) accession number: Q9N0Z4 Secondary accession number(s): Q8WMR2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 30, 2003 |
Last sequence update: | April 30, 2003 | |
Last modified: | May 25, 2022 | |
This is version 125 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families