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Protein

Protein ACTIVITY OF BC1 COMPLEX KINASE 3, chloroplastic

Gene

ABC1K3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Kinase that can phosphorylate the tocopherol cyclase VTE1, a key enzyme of tocopherol (vitamin E) metabolism and involved in the recycling of oxidated alpha-tocopherol quinone, possibly stabilizing it at plastoglobules. Regulates also membrane prenylquinone composition (PubMed:23632854). Required for photooxidative stress responses to prevent photosystem II core and chlorophyll degradations. Together with ABC1K1, contributes to plastoglobule (PG) function in prenyl-lipid metabolism, stress response, and thylakoid remodeling (PubMed:23673981, PubMed:23632854). Promotes photodamage of chloroplasts under continuous red light, thus working in opposition to ABC1K1 (PubMed:25882344).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei245ATPPROSITE-ProRule annotation1
Active sitei379Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi222 – 230ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-2753-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ACTIVITY OF BC1 COMPLEX KINASE 3, chloroplastic1 Publication (EC:2.7.-.-PROSITE-ProRule annotation, EC:2.7.11.11 Publication)
Short name:
ABC1-LIKE KINASE 31 Publication
Alternative name(s):
Protein REPRESSOR OF BDR11 Publication
Gene namesi
Name:ABC1K31 Publication
Synonyms:RBD11 Publication
Ordered Locus Names:At1g79600Imported
ORF Names:F20B17.3Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G79600
TAIRilocus:2019838 AT1G79600

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Suppression of the bleaching and dwarf phenotypes of plants lacking ABC1K1 in red light, associated with the rescue of chlorophylls and carotenoid contents as well as D1 protein level, product of psbA, one of the four core subunits of the photosystem II (PSII) (PubMed:25882344). Slight reduction of nonphotochemical quenching after high light intensity treatment. Abnormal chloroplast ultrastructure with slightly scattered thylakoid grana and reduced starch granules accumulation in normal light. In high light (HL), stronger phenotype with large scattered grana and extensive vacuolation as well as an increase in plastoglobule size and higher number of plastoglobule clusters, but no starch granule (PubMed:23632854). Impaired for the production of plastochromanol-8 (a plastoquinone-derived lipid antioxidant) and the redox recycling of alpha-tocopherol, but normal tocopherol. Increased accumulation of VTE1 and PAP1/FBN1a transcripts, but reduced levels of proteins fibrillin-1a (PAP1/FBN1a) and tocopherol cyclase (VTE1) due to protein instability and leading to abnormal accumulation of the alpha-tocopherol (alpha-T) oxidative- derivate alpha-tocopherol quinone (alpha-TQ) (PubMed:23632854). Conditional light stress phenotype in the double mutant abc1k1 abc1k3 that displays rapid chlorosis upon high light stress and slower, but irreversible, senescence-like phenotype during moderate light stress, drought or nitrogen limitation, but not cold stress. This senescence-like phenotype is associated with the degradation of the photosystem II core and up-regulation of chlorophyll degradation. During light stress, modified prenyl-lipid composition in plastoglobules (PG) probably due to reduced VTE1 activity and loss of CCD4, as well as abnormal recruitement of plastid jasmonate biosynthesis enzymes in PG (PubMed:23673981).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi82A → S in rbd1-5; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi167G → D in rbd1-6; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi243A → T in rbd1-8; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi301A → V in rbd1-3; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi320P → L in rbd1-9; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi362G → E in rbd1-11; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi399G → D in rbd1-1; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1
Mutagenesisi405P → S in rbd1-12; suppression of bdr1-1 and bdr1-2 bleaching and dwarf phenotypes. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42ChloroplastSequence analysisAdd BLAST42
ChainiPRO_000028652243 – 711Protein ACTIVITY OF BC1 COMPLEX KINASE 3, chloroplasticAdd BLAST669

Proteomic databases

PaxDbiQ9MA15
PRIDEiQ9MA15

Expressioni

Gene expression databases

ExpressionAtlasiQ9MA15 baseline and differential
GenevisibleiQ9MA15 AT

Interactioni

Subunit structurei

Interacts with ABC1K1 in plastoglobules (PG).1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G79600.1

Structurei

3D structure databases

ProteinModelPortaliQ9MA15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini216 – 546Protein kinasePROSITE-ProRule annotationAdd BLAST331

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1235 Eukaryota
COG0661 LUCA
HOGENOMiHOG000232962
InParanoidiQ9MA15
KOiK08869
OMAiAYPYFAR
OrthoDBiEOG093603L1
PhylomeDBiQ9MA15

Family and domain databases

InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR004147 UbiB_dom
PfamiView protein in Pfam
PF03109 ABC1, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9MA15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSLVVGQSLG LTLVGDGLSL RNSKINVGKS KFFSVNRRRL ARAALVQARP
60 70 80 90 100
KEDGAAASPS PSSRPASVVQ YRRADLADDL QAEARALGRA IDASIYSPEL
110 120 130 140 150
IARKHGSQPF KALRRSLEIL GALGGFALKL GIDQKQGNLE KNMKKRAIEL
160 170 180 190 200
RRIFTRLGPT FVKLGQGLST RPDLCPPDYL EELAELQDAL PTFPDAEAFA
210 220 230 240 250
CIERELDLSL ETIFSSVSPE PIAAASLGQV YKAQLRYSGQ VVAVKVQRPG
260 270 280 290 300
IEEAIGLDFY LIRGVGKLIN KYVDFITTDV LTLIDEFACR VYQELNYVQE
310 320 330 340 350
AQNARRFKKL YADKADVLVP DIFWDYTSRK VLTMEWVEGT KLNEQLAIES
360 370 380 390 400
QGLKVLDLVN TGIQCSLRQL LEYGFFHADP HPGNLLATPD GKLAFLDFGM
410 420 430 440 450
MSETPEEARF AIIGHVVHLV NRDYEAMARD YYALKFLSPD VDVTPIIPAL
460 470 480 490 500
RDFFDDALNY TVSELNFKTL VDGLGAVFYQ YPFNVPPYYA LILRSLTVLE
510 520 530 540 550
GLALYADPNF KVLAASYPYF AKRLLTDPNP YLRDALIELL FKDGKFRWNR
560 570 580 590 600
LENLLQQGSK DRDFSAKDAL QPVLKLLLDP NGEELRLLVI KEAVRVSEAI
610 620 630 640 650
ALGTVVDTYN SLPEFLRSLV FNGNGNGPLT MSTAELQSTL ELRDQVSRIW
660 670 680 690 700
GLLQSSESFD PAILQPILQV LQQPEARRLG GRVAGGVGQR LAARFLQQLL
710
RATTPSSAPS P
Length:711
Mass (Da):79,022
Last modified:October 1, 2000 - v1
Checksum:i3F1C53BBC64764CC
GO
Isoform 2 (identifier: Q9MA15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-142: Missing.

Note: No experimental confirmation available.
Show »
Length:569
Mass (Da):63,875
Checksum:iD12C2F834FF99ECD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0250671 – 142Missing in isoform 2. 1 PublicationAdd BLAST142

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC010793 Genomic DNA Translation: AAF68128.1
CP002684 Genomic DNA Translation: AEE36271.1
AY141992 mRNA Translation: AAM98256.1
AY064971 mRNA Translation: AAL57626.1
AY039869 mRNA Translation: AAK63973.1
RefSeqiNP_565214.1, NM_106608.4 [Q9MA15-1]
UniGeneiAt.14753

Genome annotation databases

EnsemblPlantsiAT1G79600.1; AT1G79600.1; AT1G79600 [Q9MA15-1]
GeneIDi844298
GrameneiAT1G79600.1; AT1G79600.1; AT1G79600 [Q9MA15-1]
KEGGiath:AT1G79600

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAB1K3_ARATH
AccessioniPrimary (citable) accession number: Q9MA15
Secondary accession number(s): Q94BV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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