UniProtKB - Q9M9F5 (NCED9_ARATH)
Protein
9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic
Gene
NCED9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Contributes probably to abscisic acid synthesis for the induction of seed dormancy.2 Publications
Catalytic activityi
- EC:1.13.11.51
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinEC:1.13.11.51
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinEC:1.13.11.51
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 357 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 406 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 471 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 642 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: TAIR
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: TAIR
- carotene catabolic process Source: GO_Central
- seed dormancy process Source: TAIR
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:AT1G78390-MONOMER |
BRENDAi | 1.13.11.51, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic (EC:1.13.11.51)Short name: AtNCED9 |
Gene namesi | Name:NCED9 Ordered Locus Names:At1g78390 ORF Names:F3F9.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G78390 |
TAIRi | locus:2032085, AT1G78390 |
Subcellular locationi
Chloroplast
- chloroplast stroma 1 Publication
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Plants exhibit abscisic-acid-deficient phenotypes in seeds, but not in vegetative tissues.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – ? | ChloroplastSequence analysis | ||
ChainiPRO_0000285998 | ? – 657 | 9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic |
Proteomic databases
PaxDbi | Q9M9F5 |
PRIDEi | Q9M9F5 |
ProteomicsDBi | 251205 |
Expressioni
Tissue specificityi
Expressed in developing siliques, embryo and endosperm.2 Publications
Developmental stagei
Expressed in seeds at early and mid-maturation stages.1 Publication
Inductioni
Low induction by drought stress.2 Publications
Gene expression databases
ExpressionAtlasi | Q9M9F5, baseline and differential |
Genevisiblei | Q9M9F5, AT |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 70 – 116 | Ser-richAdd BLAST | 47 |
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q9M9F5 |
OMAi | ENAMISH |
OrthoDBi | 524712at2759 |
PhylomeDBi | Q9M9F5 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9M9F5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTIITIISGM YIYSLLSQDA HHSQYGQNTN LVLKKPIPKP QTAAFNQEST
60 70 80 90 100
MASTTLLPST STQFLDRTFS TSSSSSRPKL QSLSFSSTLR NKKLVVPCYV
110 120 130 140 150
SSSVNKKSSV SSSLQSPTFK PPSWKKLCND VTNLIPKTTN QNPKLNPVQR
160 170 180 190 200
TAAMVLDAVE NAMISHERRR HPHPKTADPA VQIAGNFFPV PEKPVVHNLP
210 220 230 240 250
VTGTVPECIQ GVYVRNGANP LHKPVSGHHL FDGDGMVHAV RFDNGSVSYA
260 270 280 290 300
CRFTETNRLV QERECGRPVF PKAIGELHGH LGIAKLMLFN TRGLFGLVDP
310 320 330 340 350
TGGLGVANAG LVYFNGHLLA MSEDDLPYHV KVTQTGDLET SGRYDFDGQL
360 370 380 390 400
KSTMIAHPKI DPETRELFAL SYDVVSKPYL KYFRFTSDGE KSPDVEIPLD
410 420 430 440 450
QPTMIHDFAI TENFVVIPDQ QVVFRLPEMI RGGSPVVYDE KKKSRFGILN
460 470 480 490 500
KNAKDASSIQ WIEVPDCFCF HLWNSWEEPE TDEVVVIGSC MTPPDSIFNE
510 520 530 540 550
HDETLQSVLS EIRLNLKTGE STRRPVISEQ VNLEAGMVNR NLLGRKTRYA
560 570 580 590 600
YLALTEPWPK VSGFAKVDLS TGEIRKYIYG EGKYGGEPLF LPSGDGEEDG
610 620 630 640 650
GYIMVFVHDE EKVKSELQLI NAVNMKLEAT VTLPSRVPYG FHGTFISKED
LSKQALC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC013430 Genomic DNA Translation: AAF71797.1 CP002684 Genomic DNA Translation: AEE36100.1 |
PIRi | E96812 |
RefSeqi | NP_177960.1, NM_106486.3 |
Genome annotation databases
EnsemblPlantsi | AT1G78390.1; AT1G78390.1; AT1G78390 |
GeneIDi | 844175 |
Gramenei | AT1G78390.1; AT1G78390.1; AT1G78390 |
KEGGi | ath:AT1G78390 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC013430 Genomic DNA Translation: AAF71797.1 CP002684 Genomic DNA Translation: AEE36100.1 |
PIRi | E96812 |
RefSeqi | NP_177960.1, NM_106486.3 |
3D structure databases
SMRi | Q9M9F5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G78390.1 |
Proteomic databases
PaxDbi | Q9M9F5 |
PRIDEi | Q9M9F5 |
ProteomicsDBi | 251205 |
Genome annotation databases
EnsemblPlantsi | AT1G78390.1; AT1G78390.1; AT1G78390 |
GeneIDi | 844175 |
Gramenei | AT1G78390.1; AT1G78390.1; AT1G78390 |
KEGGi | ath:AT1G78390 |
Organism-specific databases
Araporti | AT1G78390 |
TAIRi | locus:2032085, AT1G78390 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q9M9F5 |
OMAi | ENAMISH |
OrthoDBi | 524712at2759 |
PhylomeDBi | Q9M9F5 |
Enzyme and pathway databases
BioCyci | MetaCyc:AT1G78390-MONOMER |
BRENDAi | 1.13.11.51, 399 |
Miscellaneous databases
PROi | PR:Q9M9F5 |
Gene expression databases
ExpressionAtlasi | Q9M9F5, baseline and differential |
Genevisiblei | Q9M9F5, AT |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED9_ARATH | |
Accessioni | Q9M9F5Primary (citable) accession number: Q9M9F5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families