UniProtKB - Q9M6E8 (NCED1_PHAVU)
Protein
9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic
Gene
NCED1
Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids, in response to water stress. Active on 9-cis-violaxanthin and 9'-cis-neoxanthin, but not on the all-trans isomers of violaxanthin and neoxanthin.1 Publication
Miscellaneous
Overexpression of NCED1 results in increased accumulation of abscisic acid and resistance to water stress.
Catalytic activityi
- EC:1.13.11.51
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinEC:1.13.11.51
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinEC:1.13.11.51
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 316 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 365 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 430 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 602 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: UniProtKB-KW
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis, Stress response |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.13.11.51, 4746 |
Chemistry databases
SwissLipidsi | SLP:000001488 |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic (EC:1.13.11.51)Alternative name(s): PvNCED1 |
Gene namesi | Name:NCED1 |
Organismi | Phaseolus vulgaris (Kidney bean) (French bean) |
Taxonomic identifieri | 3885 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › fabids › Fabales › Fabaceae › Papilionoideae › 50 kb inversion clade › NPAAA clade › indigoferoid/millettioid clade › Phaseoleae › Phaseolus |
Subcellular locationi
Chloroplast
- chloroplast thylakoid membrane 1 Publication
Chloroplast
- chloroplast thylakoid membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, ThylakoidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 41 | ChloroplastSequence analysisAdd BLAST | 41 | |
ChainiPRO_0000285992 | 42 – 615 | 9-cis-epoxycarotenoid dioxygenase NCED1, chloroplasticAdd BLAST | 574 |
Expressioni
Inductioni
By drought stress.1 Publication
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 571 – 592 | Sequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 46 – 91 | Thr-richAdd BLAST | 46 |
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Coiled coil, Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9M6E8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSPASNTWI NTTLPSSCSS PFKDLASTSS SPTTLLPFKK RSSSNTNTIT
60 70 80 90 100
CSLQTLHYPK QYQPTSTSTT TTPTPIKPTT TTTTTTPHRE TKPLSDTKQP
110 120 130 140 150
FPQKWNFLQK AAATGLDMVE TALVSHESKH PLPKTADPKV QIAGNFAPVP
160 170 180 190 200
EHAADQALPV VGKIPKCIDG VYVRNGANPL YEPVAGHHFF DGDGMVHAVK
210 220 230 240 250
FTNGAASYAC RFTETQRLAQ EKSLGRPVFP KAIGELHGHS GIARLLLFYA
260 270 280 290 300
RSLFQLVDGS HGMGVANAGL VYFNNHLLAM SEDDLPYHVR ITSNGDLTTV
310 320 330 340 350
GRYDFNGQLN STMIAHPKLD PVNGDLHALS YDVVQKPYLK YFRFSADGVK
360 370 380 390 400
SPDVEIPLKE PTMMHDFAIT ENFVVVPDQQ VVFKLTEMIT GGSPVVYDKN
410 420 430 440 450
KTSRFGILDK NAKDANAMRW IDAPECFCFH LWNAWEEPET DEIVVIGSCM
460 470 480 490 500
TPADSIFNEC DESLKSVLSE IRLNLRTGKS TRRPIISDAE QVNLEAGMVN
510 520 530 540 550
RNKLGRKTQF AYLALAEPWP KVSGFAKVDL FSGEVQKYMY GEEKFGGEPL
560 570 580 590 600
FLPNGEEEGD GYILAFVHDE KEWKSELQIV NAQNLKLEAS IKLPSRVPYG
610
FHGTFIHSKD LRKQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190462 mRNA Translation: AAF26356.1 |
Genome annotation databases
KEGGi | ag:AAF26356 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF190462 mRNA Translation: AAF26356.1 |
3D structure databases
SMRi | Q9M6E8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3885.XP_007149219.1 |
Chemistry databases
SwissLipidsi | SLP:000001488 |
Genome annotation databases
KEGGi | ag:AAF26356 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
Enzyme and pathway databases
BRENDAi | 1.13.11.51, 4746 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED1_PHAVU | |
Accessioni | Q9M6E8Primary (citable) accession number: Q9M6E8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | October 1, 2000 | |
Last modified: | December 2, 2020 | |
This is version 53 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |