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Protein

DNA (cytosine-5)-methyltransferase DRM2

Gene

DRM2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in de novo DNA methylation. Controls asymmetric and CpNpG methylation. Required for FWA gene silencing but not for the maintenance of SUP gene silencing. Functionally redundant to CMT3 to maintain non-CpG methylation. Involved in RNA-directed DNA methylation (RdDM) (PubMed:12121623, PubMed:12151602, PubMed:14680640). Acts as major DNA methyltransferase in the RdDM pathway, and is essential for RNA-directed de novo DNA methylation of cytosines in all sequence contexts (PubMed:21060858, PubMed:21212233). Associates with long non-coding RNA (lncRNA) produced by RNA polymerase V (Pol V). This association is dependent on AGO4 and IDN2, and results in DNA methylation of RdDM target loci (PubMed:24862207).6 Publications

Miscellaneous

DRM2 is expressed at much higher levels than DRM1, which is scarcely detected, suggesting that DRM2 is the predominant de novo methylase.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  • DNA binding Source: UniProtKB-KW
  • DNA-methyltransferase activity Source: CACAO

GO - Biological processi

  • defense response to fungus Source: TAIR
  • DNA methylation Source: TAIR
  • gene silencing Source: UniProtKB
  • histone H3-K9 methylation Source: TAIR

Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi4601 M.AthDRM2

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase DRM2 (EC:2.1.1.37)
Alternative name(s):
Protein DOMAINS REARRANGED METHYLASE 2
Gene namesi
Name:DRM2
Ordered Locus Names:At5g14620/At5g14630
ORF Names:T15N1.110/T15N1.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G14620
TAIRilocus:2222627 AT5G14620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi585S → A: Loss of function in maintaining non-CpG methylation. 1 Publication1
Mutagenesisi587C → A: Loss of function in maintaining non-CpG methylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003819421 – 626DNA (cytosine-5)-methyltransferase DRM2Add BLAST626

Proteomic databases

PaxDbiQ9M548
PRIDEiQ9M548

PTM databases

iPTMnetiQ9M548

Expressioni

Tissue specificityi

Expressed in roots, inflorescences and at lower levels in leaves.1 Publication

Gene expression databases

ExpressionAtlasiQ9M548 baseline and differential
GenevisibleiQ9M548 AT

Interactioni

Subunit structurei

Interacts with RDM1.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi16592, 2 interactors
DIPiDIP-59279N
IntActiQ9M548, 9 interactors
MINTiQ9M548
STRINGi3702.AT5G14620.1

Structurei

3D structure databases

ProteinModelPortaliQ9M548
SMRiQ9M548
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 101UBA 1PROSITE-ProRule annotationAdd BLAST43
Domaini109 – 150UBA 2PROSITE-ProRule annotationAdd BLAST42
Domaini190 – 232UBA 3PROSITE-ProRule annotationAdd BLAST43
Domaini295 – 626SAM-dependent MTase DRM-typePROSITE-ProRule annotationAdd BLAST332

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. DRM-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IGHW Eukaryota
ENOG410XQ4Y LUCA
HOGENOMiHOG000030355
InParanoidiQ9M548
OMAiPNTIHEA
OrthoDBiEOG093605J3
PhylomeDBiQ9M548

Family and domain databases

InterProiView protein in InterPro
IPR001525 C5_MeTfrase
IPR029063 SAM-dependent_MTases
IPR030380 SAM_MeTfrase_DRM
IPR015940 UBA
IPR009060 UBA-like_sf
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 3 hits
SUPFAMiSSF46934 SSF46934, 3 hits
SSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51680 SAM_MT_DRM, 1 hit
PS50030 UBA, 2 hits

Sequencei

Sequence statusi: Complete.

Q9M548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVIWNNDDDD FLEIDNFQSS PRSSPIHAMQ CRVENLAGVA VTTSSLSSPT
60 70 80 90 100
ETTDLVQMGF SDEVFATLFD MGFPVEMISR AIKETGPNVE TSVIIDTISK
110 120 130 140 150
YSSDCEAGSS KSKAIDHFLA MGFDEEKVVK AIQEHGEDNM EAIANALLSC
160 170 180 190 200
PEAKKLPAAV EEEDGIDWSS SDDDTNYTDM LNSDDEKDPN SNENGSKIRS
210 220 230 240 250
LVKMGFSELE ASLAVERCGE NVDIAELTDF LCAAQMAREF SEFYTEHEEQ
260 270 280 290 300
KPRHNIKKRR FESKGEPRSS VDDEPIRLPN PMIGFGVPNE PGLITHRSLP
310 320 330 340 350
ELARGPPFFY YENVALTPKG VWETISRHLF EIPPEFVDSK YFCVAARKRG
360 370 380 390 400
YIHNLPINNR FQIQPPPKYT IHDAFPLSKR WWPEWDKRTK LNCILTCTGS
410 420 430 440 450
AQLTNRIRVA LEPYNEEPEP PKHVQRYVID QCKKWNLVWV GKNKAAPLEP
460 470 480 490 500
DEMESILGFP KNHTRGGGMS RTERFKSLGN SFQVDTVAYH LSVLKPIFPH
510 520 530 540 550
GINVLSLFTG IGGGEVALHR LQIKMKLVVS VEISKVNRNI LKDFWEQTNQ
560 570 580 590 600
TGELIEFSDI QHLTNDTIEG LMEKYGGFDL VIGGSPCNNL AGGNRVSRVG
610 620
LEGDQSSLFF EYCRILEVVR ARMRGS
Length:626
Mass (Da):70,431
Last modified:October 1, 2000 - v1
Checksum:i10A38FC068636DB7
GO

Sequence cautioni

The sequence CAB87629 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g14620 and At5g14630.Curated
The sequence CAB87630 differs from that shown. Reason: Erroneous gene model prediction. Was originally thought to correspond to two different genes At5g14620 and At5g14630.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF240695 mRNA Translation: AAF66129.1
AL163792 Genomic DNA Translation: CAB87629.1 Sequence problems.
AL163792 Genomic DNA Translation: CAB87630.1 Sequence problems.
CP002688 Genomic DNA Translation: AED92056.1
AK176138 mRNA Translation: BAD43901.1
AK220953 mRNA Translation: BAD94486.1
AK229197 mRNA Translation: BAF01067.1
PIRiT48635
T48636
RefSeqiNP_196966.2, NM_121466.3
UniGeneiAt.5084

Genome annotation databases

EnsemblPlantsiAT5G14620.1; AT5G14620.1; AT5G14620
GeneIDi831315
GrameneiAT5G14620.1; AT5G14620.1; AT5G14620
KEGGiath:AT5G14620

Similar proteinsi

Entry informationi

Entry nameiDRM2_ARATH
AccessioniPrimary (citable) accession number: Q9M548
Secondary accession number(s): Q9LYJ7, Q9LYJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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