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Protein

Plastidial pyruvate kinase 4, chloroplastic

Gene

PKP4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g63250), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (At3g25960), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At5g08490), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei265SubstrateBy similarity1
Metal bindingi267PotassiumBy similarity1
Metal bindingi299PotassiumBy similarity1
Binding sitei583Substrate; via amide nitrogenBy similarity1
Sitei583Transition state stabilizerBy similarity1
Metal bindingi585MagnesiumBy similarity1
Binding sitei614Substrate; via amide nitrogenBy similarity1
Metal bindingi615MagnesiumBy similarity1
Binding sitei615Substrate; via amide nitrogenBy similarity1
Binding sitei647SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciARA:AT3G49160-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Plastidial pyruvate kinase 4, chloroplastic (EC:2.7.1.40)
Short name:
PKp4
Gene namesi
Name:PKP4
Ordered Locus Names:At3g49160
ORF Names:F2K15.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G49160
TAIRilocus:2082866 AT3G49160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000416990? – 710Plastidial pyruvate kinase 4, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PaxDbiQ9M3B6
PRIDEiQ9M3B6

Expressioni

Gene expression databases

ExpressionAtlasiQ9M3B6 differential
GenevisibleiQ9M3B6 AT

Interactioni

Protein-protein interaction databases

BioGridi9395, 8 interactors
IntActiQ9M3B6, 7 interactors
STRINGi3702.AT3G49160.1

Structurei

3D structure databases

ProteinModelPortaliQ9M3B6
SMRiQ9M3B6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000224464
InParanoidiQ9M3B6
KOiK00873
OMAiNCAHDDE
OrthoDBiEOG09360398
PhylomeDBiQ9M3B6

Family and domain databases

Gene3Di2.40.33.10, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR015793 Pyrv_Knase_brl
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 2 hits
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M3B6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGLDSSHLL RDKILCFSSR SHINNQHKKT SYALSLNHMK LPIQRTLAFA
60 70 80 90 100
LARGKGEAES FSRLEATFGD NTSTECTWSF DFPDSKDAMS HLKSEADLSG
110 120 130 140 150
SNGANNVASV IEKLNALRSH LLAAEKWNAS QLHLCDSKYL ECATNLVHYM
160 170 180 190 200
ALRSLDIEQL NSHLASLGLS SLDNNNLDVL AHLNASINLL MNDQNAVTES
210 220 230 240 250
WTNVYPKGKS TKKNDKGRVL SYKESLLGKL REGRSTHIMV TIGEEATLSE
260 270 280 290 300
TFITDILKAG TSVIRINCAH GDPSIWGEII KRVRRTSQML EMPCRVHMDL
310 320 330 340 350
AGPKLRTGTL KPGPCVMKIS PKKDAYGNVV SPALVWLCLT GTEPPAHVSP
360 370 380 390 400
DATISVQGQD FLAGLQIGDS IRLCDARGRK RRLKISKEFH VFNSTGFVAE
410 420 430 440 450
CFDTAYIESG TELSVKGKKG RRLVGRVVDV PPKESFVRLK VGDLLVITRE
460 470 480 490 500
GSLDEPSVTV PGAHRLTCPS GYLFDSVKPG ETIGFDDGKI WGVIKGTSPS
510 520 530 540 550
EVIVSITHAR PKGTKLGSEK SINIPQSDIH FKGLTSKDIK DLDYVASHAD
560 570 580 590 600
MVGISFIRDV HDITVLRQEL KKRKLDDLGI VLKIETKSGF KNLSLILLEA
610 620 630 640 650
MKCSNPLGIM IARGDLAVEC GWERLANMQE EIIAICKAAR VPVIMATQVL
660 670 680 690 700
ESLVKSGVPT RAEITDAANA KRASCVMLNK GKNIVEAVSM LDTILHTKLI
710
YKKSDSENLH
Length:710
Mass (Da):77,827
Last modified:October 1, 2000 - v1
Checksum:i515D788AD6609E70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL132956 Genomic DNA Translation: CAB66395.1
CP002686 Genomic DNA Translation: AEE78506.1
AY072177 mRNA Translation: AAL59999.1
AY096527 mRNA Translation: AAM20177.1
PIRiT45821
RefSeqiNP_190485.1, NM_114775.3
UniGeneiAt.35642

Genome annotation databases

EnsemblPlantsiAT3G49160.1; AT3G49160.1; AT3G49160
GeneIDi824077
GrameneiAT3G49160.1; AT3G49160.1; AT3G49160
KEGGiath:AT3G49160

Similar proteinsi

Entry informationi

Entry nameiPKP4_ARATH
AccessioniPrimary (citable) accession number: Q9M3B6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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