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Protein

Endochitinase EP3

Gene

EP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably involved in hypersensitive reaction upon Xanthomonas campestris infection.1 Publication

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.By similarity

pH dependencei

Optimum pH is 4.8.1 Publication

GO - Molecular functioni

  • chitinase activity Source: TAIR
  • chitin binding Source: UniProtKB-KW

GO - Biological processi

  • cell wall macromolecule catabolic process Source: InterPro
  • chitin catabolic process Source: UniProtKB-KW
  • defense response Source: UniProtKB-KW
  • polysaccharide catabolic process Source: UniProtKB-KW
  • response to bacterium Source: UniProtKB
  • response to wounding Source: UniProtKB
  • somatic embryogenesis Source: TAIR

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation
LigandChitin-binding

Enzyme and pathway databases

BioCyciARA:AT3G54420-MONOMER

Protein family/group databases

CAZyiCBM18 Carbohydrate-Binding Module Family 18
GH19 Glycoside Hydrolase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase EP31 Publication (EC:3.2.1.14)
Alternative name(s):
Chitinase class IV1 Publication
Short name:
AtchitIV1 Publication
Protein HOMOLOG OF CARROT EP3-3 CHITINASE1 Publication
Short name:
AtEP31 Publication
Gene namesi
Name:EP31 Publication
Synonyms:CHIV1 Publication
Ordered Locus Names:At3g54420Imported
ORF Names:T12E18.110Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54420
TAIRilocus:2096159 AT3G54420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000043391129 – 273Endochitinase EP3Sequence analysisAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi24N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi31 ↔ 39PROSITE-ProRule annotation
Disulfide bondi33 ↔ 45PROSITE-ProRule annotation
Disulfide bondi38 ↔ 52PROSITE-ProRule annotation
Glycosylationi47N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi56 ↔ 61PROSITE-ProRule annotation
Glycosylationi157N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi270N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9M2U5
PRIDEiQ9M2U5

Expressioni

Tissue specificityi

Expressed in cells surrounding embryos, stems, seedlings, pollen, roots, shoots, inflorescence, flowers, siliques and leaves (PubMed:11525512, PubMed:19420714). Present in seedpods and seed embryos, but not in roots, inflorescence stems, leaves and flowers (PubMed:9426222).3 Publications

Developmental stagei

Expressed during somatic embryogenesis in nursing cells surrounding the embryos but not in embryos. Accumulates in mature pollen and growing pollen tubes until they enter the receptive synergid, but not in endosperm and integuments. In adult plants, present in hydathodes, stipules, root epidermis and emerging root hairs.1 Publication

Inductioni

Accumulates rapidly and transiently in leaves after inoculation with Xanthomonas campestris (PubMed:9426222). Slightly repressed by wounding (PubMed:19420714).2 Publications

Gene expression databases

ExpressionAtlasiQ9M2U5 baseline and differential
GenevisibleiQ9M2U5 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G54420.1

Structurei

3D structure databases

ProteinModelPortaliQ9M2U5
SMRiQ9M2U5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 63Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 273CatalyticBy similarityAdd BLAST204

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742 Eukaryota
COG3979 LUCA
HOGENOMiHOG000231411
InParanoidiQ9M2U5
KOiK01183
OMAiSFKSALW
OrthoDBiEOG09360MAQ
PhylomeDBiQ9M2U5

Family and domain databases

CDDicd00325 chitinase_glyco_hydro_19, 1 hit
Gene3Di3.30.60.10, 1 hit
InterProiView protein in InterPro
IPR001002 Chitin-bd_1
IPR018371 Chitin-binding_1_CS
IPR036861 Endochitinase-like_sf
IPR016283 Glyco_hydro_19
IPR000726 Glyco_hydro_19_cat
IPR023346 Lysozyme-like_dom_sf
PfamiView protein in Pfam
PF00187 Chitin_bind_1, 1 hit
PF00182 Glyco_hydro_19, 2 hits
PIRSFiPIRSF001060 Endochitinase, 1 hit
PRINTSiPR00451 CHITINBINDNG
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609 Chitin_bd_1, 1 hit
SMARTiView protein in SMART
SM00270 ChtBD1, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF57016 SSF57016, 1 hit
PROSITEiView protein in PROSITE
PS00026 CHIT_BIND_I_1, 1 hit
PS50941 CHIT_BIND_I_2, 1 hit
PS00773 CHITINASE_19_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9M2U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTPTISKSI SLVTILLVLQ AFSNTTKAQN CGCSSELCCS QFGFCGNTSD
60 70 80 90 100
YCGVGCQQGP CFAPPPANGV SVAEIVTQEF FNGIISQAAS SCAGNRFYSR
110 120 130 140 150
GAFLEALDSY SRFGRVGSTD DSRREIAAFF AHVTHETGHF CYIEEIDGAS
160 170 180 190 200
KDYCDENATQ YPCNPNKGYY GRGPIQLSWN FNYGPAGTAI GFDGLNAPET
210 220 230 240 250
VATDPVISFK TALWYWTNRV QPVISQGFGA TIRAINGALE CDGANTATVQ
260 270
ARVRYYTDYC RQLGVDPGNN LTC
Length:273
Mass (Da):29,436
Last modified:October 1, 2000 - v1
Checksum:i1DB0A002F4EF44EC
GO

Sequence cautioni

The sequence CAA81243 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139H → RN in CAA74930 (PubMed:9426222).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14590 Genomic DNA Translation: CAA74930.1
AL132971 Genomic DNA Translation: CAB81807.1
CP002686 Genomic DNA Translation: AEE79228.1
BT010422 mRNA Translation: AAQ62423.1
AK176488 mRNA Translation: BAD44251.1
Z26409 mRNA Translation: CAA81243.1 Different initiation.
PIRiT47601
RefSeqiNP_191010.1, NM_115302.3
UniGeneiAt.35109

Genome annotation databases

EnsemblPlantsiAT3G54420.1; AT3G54420.1; AT3G54420
GeneIDi824608
GrameneiAT3G54420.1; AT3G54420.1; AT3G54420
KEGGiath:AT3G54420

Similar proteinsi

Entry informationi

Entry nameiCHI5_ARATH
AccessioniPrimary (citable) accession number: Q9M2U5
Secondary accession number(s): O23248, Q42085
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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