UniProtKB - Q9M156 (U72B1_ARATH)
UDP-glycosyltransferase 72B1
UGT72B1
Functioni
Bifunctional O-glycosyltransferase and N-glycosyltransferase that can detoxify xenobiotics. Possesses high activity to metabolize the persistent pollutants 2,4,5-trichlorophenol (TCP) and 3,4-dichloroaniline (DCA). Also active on benzoates and benzoate derivatives in vitro.
3 PublicationsCatalytic activityi
- EC:2.4.1.218
Kineticsi
- KM=34.3 µM for 3,4-dichlorophenol1 Publication
- KM=16.5 µM for 3,4-dichloroaniline1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 277 | UDP-alpha-D-glucoseCombined sources1 Publication | 1 |
GO - Molecular functioni
- hydroquinone glucosyltransferase activity Source: UniProtKB-EC
- UDP-glucosyltransferase activity Source: TAIR
- UDP-glycosyltransferase activity Source: TAIR
GO - Biological processi
- lignin biosynthetic process Source: TAIR
- response to toxic substance Source: TAIR
- xenobiotic catabolic process Source: TAIR
- xenobiotic metabolic process Source: TAIR
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Detoxification |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:UGT72B1 Ordered Locus Names:At4g01070 ORF Names:F2N1.15 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G01070 |
TAIRi | locus:2125023, AT4G01070 |
Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 14 | S → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 19 | H → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 19 | H → Q: Reduces N-glycosyltransferase activity. Loss of O-glycosyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 117 | D → A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 312 | N → D: Decreases activity. Loss of N-glycosyltransferase activity; when associated with F-315. 1 Publication | 1 | |
Mutagenesisi | 315 | Y → F: Decreases activity. Loss of N-glycosyltransferase activity; when associated with D-312. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074160 | 1 – 480 | UDP-glycosyltransferase 72B1Add BLAST | 480 |
Proteomic databases
PaxDbi | Q9M156 |
PRIDEi | Q9M156 |
ProteomicsDBi | 242611 [Q9M156-1] |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9M156, baseline and differential |
Genevisiblei | Q9M156, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 13203, 1 interactor |
STRINGi | 3702.AT4G01070.1 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9M156 |
SMRi | Q9M156 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9M156 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 346 – 347 | UDP-alpha-D-glucose bindingCombined sources1 Publication | 2 | |
Regioni | 364 – 372 | UDP-alpha-D-glucose bindingCombined sources1 Publication | 9 | |
Regioni | 386 – 389 | UDP-alpha-D-glucose bindingCombined sources1 Publication | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1192, Eukaryota |
HOGENOMi | CLU_001724_3_2_1 |
InParanoidi | Q9M156 |
PhylomeDBi | Q9M156 |
Family and domain databases
CDDi | cd03784, GT1_Gtf-like, 1 hit |
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEESKTPHVA IIPSPGMGHL IPLVEFAKRL VHLHGLTVTF VIAGEGPPSK
60 70 80 90 100
AQRTVLDSLP SSISSVFLPP VDLTDLSSST RIESRISLTV TRSNPELRKV
110 120 130 140 150
FDSFVEGGRL PTALVVDLFG TDAFDVAVEF HVPPYIFYPT TANVLSFFLH
160 170 180 190 200
LPKLDETVSC EFRELTEPLM LPGCVPVAGK DFLDPAQDRK DDAYKWLLHN
210 220 230 240 250
TKRYKEAEGI LVNTFFELEP NAIKALQEPG LDKPPVYPVG PLVNIGKQEA
260 270 280 290 300
KQTEESECLK WLDNQPLGSV LYVSFGSGGT LTCEQLNELA LGLADSEQRF
310 320 330 340 350
LWVIRSPSGI ANSSYFDSHS QTDPLTFLPP GFLERTKKRG FVIPFWAPQA
360 370 380 390 400
QVLAHPSTGG FLTHCGWNST LESVVSGIPL IAWPLYAEQK MNAVLLSEDI
410 420 430 440 450
RAALRPRAGD DGLVRREEVA RVVKGLMEGE EGKGVRNKMK ELKEAACRVL
460 470 480
KDDGTSTKAL SLVALKWKAH KKELEQNGNH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JHS0 | F4JHS0_ARATH | UDP-Glycosyltransferase superfamily... | GT72B1 UGT72B1, At4g01070, F2N1.15, F2N1_15 | 349 | Annotation score: |
Sequence cautioni
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF007269 Genomic DNA Translation: AAB61023.1 Sequence problems. AL161491 Genomic DNA Translation: CAB80916.1 CP002687 Genomic DNA Translation: AEE81977.1 AF360262 mRNA Translation: AAK25972.1 AY040075 mRNA Translation: AAK64133.1 AY084892 mRNA Translation: AAM61455.1 |
PIRi | B85014 T01732 |
RefSeqi | NP_192016.1, NM_116337.3 [Q9M156-1] |
Genome annotation databases
EnsemblPlantsi | AT4G01070.1; AT4G01070.1; AT4G01070 [Q9M156-1] |
GeneIDi | 827912 |
Gramenei | AT4G01070.1; AT4G01070.1; AT4G01070 [Q9M156-1] |
KEGGi | ath:AT4G01070 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF007269 Genomic DNA Translation: AAB61023.1 Sequence problems. AL161491 Genomic DNA Translation: CAB80916.1 CP002687 Genomic DNA Translation: AEE81977.1 AF360262 mRNA Translation: AAK25972.1 AY040075 mRNA Translation: AAK64133.1 AY084892 mRNA Translation: AAM61455.1 |
PIRi | B85014 T01732 |
RefSeqi | NP_192016.1, NM_116337.3 [Q9M156-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2VCE | X-ray | 1.90 | A | 1-480 | [»] | |
2VCH | X-ray | 1.45 | A | 1-480 | [»] | |
2VG8 | X-ray | 1.75 | A | 1-480 | [»] | |
AlphaFoldDBi | Q9M156 | |||||
SMRi | Q9M156 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 13203, 1 interactor |
STRINGi | 3702.AT4G01070.1 |
Protein family/group databases
CAZyi | GT1, Glycosyltransferase Family 1 |
Proteomic databases
PaxDbi | Q9M156 |
PRIDEi | Q9M156 |
ProteomicsDBi | 242611 [Q9M156-1] |
Protocols and materials databases
DNASUi | 827912 |
Genome annotation databases
EnsemblPlantsi | AT4G01070.1; AT4G01070.1; AT4G01070 [Q9M156-1] |
GeneIDi | 827912 |
Gramenei | AT4G01070.1; AT4G01070.1; AT4G01070 [Q9M156-1] |
KEGGi | ath:AT4G01070 |
Organism-specific databases
Araporti | AT4G01070 |
TAIRi | locus:2125023, AT4G01070 |
Phylogenomic databases
eggNOGi | KOG1192, Eukaryota |
HOGENOMi | CLU_001724_3_2_1 |
InParanoidi | Q9M156 |
PhylomeDBi | Q9M156 |
Miscellaneous databases
EvolutionaryTracei | Q9M156 |
PROi | PR:Q9M156 |
Gene expression databases
ExpressionAtlasi | Q9M156, baseline and differential |
Genevisiblei | Q9M156, AT |
Family and domain databases
CDDi | cd03784, GT1_Gtf-like, 1 hit |
InterProi | View protein in InterPro IPR002213, UDP_glucos_trans IPR035595, UDP_glycos_trans_CS |
Pfami | View protein in Pfam PF00201, UDPGT, 1 hit |
PROSITEi | View protein in PROSITE PS00375, UDPGT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | U72B1_ARATH | |
Accessioni | Q9M156Primary (citable) accession number: Q9M156 Secondary accession number(s): O04622 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 12, 2003 |
Last sequence update: | October 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families