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Entry version 149 (16 Oct 2019)
Sequence version 2 (01 Feb 2003)
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Protein

Vesicle-fusing ATPase

Gene

NSF

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in vesicle-mediated transport. The ATPase activity of NSF serves to disassemble the SNARE complex, freeing the components for subsequent pairing and fusion events.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi544MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi499 – 504ATPBy similarity6
Nucleotide bindingi539 – 546ATPBy similarity8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase
Biological processER-Golgi transport, Protein transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
ARA:AT4G04910-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Vesicular-fusion protein NSF
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:NSF
Ordered Locus Names:At4g04910
ORF Names:T1J1.4
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiArabidopsis thaliana (Mouse-ear cress)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3702 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000006548 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 4

Organism-specific databases

Arabidopsis Information Portal

More...
Araporti
AT4G04910

The Arabidopsis Information Resource

More...
TAIRi
locus:2135378 AT4G04910

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000845681 – 742Vesicle-fusing ATPaseAdd BLAST742

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q9M0Y8

PRoteomics IDEntifications database

More...
PRIDEi
Q9M0Y8

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q9M0Y8

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
Q9M0Y8 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
Q9M0Y8 AT

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Hexamer. Binds to SNARE-SNAP complexes to form 20S particles (By similarity).

By similarity

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
11141, 8 interactors

Protein interaction database and analysis system

More...
IntActi
Q9M0Y8, 4 interactors

STRING: functional protein association networks

More...
STRINGi
3702.AT4G04910.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q9M0Y8

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0741 Eukaryota
COG0464 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000198544

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q9M0Y8

KEGG Orthology (KO)

More...
KOi
K06027

Identification of Orthologs from Complete Genome Data

More...
OMAi
RMRAIKC

Database of Orthologous Groups

More...
OrthoDBi
197562at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q9M0Y8

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003593 AAA+_ATPase
IPR041569 AAA_lid_3
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
IPR039812 Vesicle-fus_ATPase

The PANTHER Classification System

More...
PANTHERi
PTHR23078 PTHR23078, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00004 AAA, 2 hits
PF17862 AAA_lid_3, 1 hit
PF02933 CDC48_2, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00382 AAA, 2 hits
SM01073 CDC48_N, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00674 AAA, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q9M0Y8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGRYGSQVM TMTVTNTPSA DLAFTNLAYC SSSDLRQFSV PGSDLFLANV
60 70 80 90 100
ADSFILSLCG HGSIRDGNIA LNAIQRRHAR VSTGDMVSVS RFVPPENFDL
110 120 130 140 150
AMLTLELEFV KKGTKSEQVD AALLSTQLKR KYTNQVLTVG QKATFEYHGT
160 170 180 190 200
NYILTVNRAD VEGQDHTNGI ERGLLSKDTY IVFEASNASG IKIVNQREAA
210 220 230 240 250
SSNIFKHKEF NLESLGIGGL GAEFADIFRR AFASRVFPPH VTSRLGIKHV
260 270 280 290 300
KGMLLFGPPG TGKTLMARQI GKMLNGKDPK IVNGPEVLSK FVGETEKNVR
310 320 330 340 350
DLFADAEQDQ RTLGDASELH VIIFDEIDAI CKSRGSTRDG TGVHDSIVNQ
360 370 380 390 400
LLTKIDGVEA LNNVLLIGMT NRKDLLDEAL LRPGRLEVQV EISLPDEAGR
410 420 430 440 450
LQILQIHTNK MKENSFLGTD INLQELAART KNYSGAELEG VVKSATSYAL
460 470 480 490 500
NRQLSMDDLT KPVEEENIKI TMEDFLHAIY EVQPAFGAST DDLERCRLNG
510 520 530 540 550
MVDCGHRHNH IYKRAMLLVE QVKVSTRSPL VTCLLEGPSG SGKTALAATI
560 570 580 590 600
GIDSDFPYVK IVSAETMIGL SESTKCAHIV KVFEDAYKSP MSIIILDDIE
610 620 630 640 650
RLLEFIAIGP RFSNIISQTL MVLLKRLPPK GKKLLVFGTT SEVTFLESVG
660 670 680 690 700
ISDCFSVTHS VPTLQKEDAK KVLNQLNLFS EDDVDSAAEA LNDMPIKKIY
710 720 730 740
MLIEMAAQGE NGGSAEAIYA GREKININHF YDCLGDFIRF TG
Length:742
Mass (Da):81,487
Last modified:February 1, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i39611058DEAC4A32
GO

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAD17345 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB81033 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF128393 Genomic DNA Translation: AAD17345.1 Sequence problems.
AL161502 Genomic DNA Translation: CAB81033.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82440.1
AY102111 mRNA Translation: AAM26681.1

Protein sequence database of the Protein Information Resource

More...
PIRi
G85061

NCBI Reference Sequences

More...
RefSeqi
NP_192400.2, NM_116729.4

Genome annotation databases

Ensembl plant genome annotation project

More...
EnsemblPlantsi
AT4G04910.1; AT4G04910.1; AT4G04910

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
825830

Gramene; a comparative resource for plants

More...
Gramenei
AT4G04910.1; AT4G04910.1; AT4G04910

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ath:AT4G04910

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128393 Genomic DNA Translation: AAD17345.1 Sequence problems.
AL161502 Genomic DNA Translation: CAB81033.1 Sequence problems.
CP002687 Genomic DNA Translation: AEE82440.1
AY102111 mRNA Translation: AAM26681.1
PIRiG85061
RefSeqiNP_192400.2, NM_116729.4

3D structure databases

SMRiQ9M0Y8
ModBaseiSearch...

Protein-protein interaction databases

BioGridi11141, 8 interactors
IntActiQ9M0Y8, 4 interactors
STRINGi3702.AT4G04910.1

PTM databases

iPTMnetiQ9M0Y8

Proteomic databases

PaxDbiQ9M0Y8
PRIDEiQ9M0Y8

Genome annotation databases

EnsemblPlantsiAT4G04910.1; AT4G04910.1; AT4G04910
GeneIDi825830
GrameneiAT4G04910.1; AT4G04910.1; AT4G04910
KEGGiath:AT4G04910

Organism-specific databases

AraportiAT4G04910
TAIRilocus:2135378 AT4G04910

Phylogenomic databases

eggNOGiKOG0741 Eukaryota
COG0464 LUCA
HOGENOMiHOG000198544
InParanoidiQ9M0Y8
KOiK06027
OMAiRMRAIKC
OrthoDBi197562at2759
PhylomeDBiQ9M0Y8

Enzyme and pathway databases

BioCyciARA:AT4G04910-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q9M0Y8

Gene expression databases

ExpressionAtlasiQ9M0Y8 baseline and differential
GenevisibleiQ9M0Y8 AT

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR041569 AAA_lid_3
IPR009010 Asp_de-COase-like_dom_sf
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR004201 Cdc48_dom2
IPR029067 CDC48_domain_2-like_sf
IPR003338 CDC4_N-term_subdom
IPR027417 P-loop_NTPase
IPR039812 Vesicle-fus_ATPase
PANTHERiPTHR23078 PTHR23078, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 2 hits
PF17862 AAA_lid_3, 1 hit
PF02933 CDC48_2, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01073 CDC48_N, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
SSF52540 SSF52540, 2 hits
SSF54585 SSF54585, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNSF_ARATH
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9M0Y8
Secondary accession number(s): Q9ZPH6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: October 16, 2019
This is version 149 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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