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Protein

Leucine-rich repeat receptor protein kinase EMS1

Gene

EMS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor with a serine/threonine-protein kinase activity required for the specification of the correct number of male archesporial initials and for the subsequent specification of tapetal and middle cell layer identities. In seeds, required for enhancing cell size and the rate of embryonic development.

Miscellaneous

Some ecotypic variation may occur: in cv. Landsberg erecta, meiocytes of a null mutant fail to undergo cytokinesis while in cv. C24, cytokinesis clearly takes place, with the mutant meiocytes degenerating shortly after the tetrad stage.
In cv. C24, the gene is expressed in the young ovular primordia, but the protein is not present in these organs.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei945ATPPROSITE-ProRule annotation1
Active sitei1043Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi923 – 931ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • anther wall tapetum cell fate specification Source: TAIR
  • microsporogenesis Source: TAIR
  • protein autophosphorylation Source: TAIR

Keywordsi

Molecular functionKinase, Receptor, Serine/threonine-protein kinase, Transferase
Biological processMeiosis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat receptor protein kinase EMS1Curated (EC:2.7.11.1)
Alternative name(s):
Protein EXCESS MICROSPOROCYTES 11 Publication
Protein EXTRA SPOROGENOUS CELLS1 Publication
Gene namesi
Name:EMS11 Publication
Synonyms:ESP, EXS1 Publication
Ordered Locus Names:At5g07280
ORF Names:T28J14.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G07280
TAIRilocus:2182870 AT5G07280

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei828 – 848HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4L → LIL in rev.1; no effect. 2 Publications1
Mutagenesisi4L → LSIL in rev.2; no effect. 2 Publications1
Mutagenesisi104K → N in exs-2; male sterility. 2 Publications1
Mutagenesisi1185V → E in exs-1; male sterility. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002433119 – 1192Leucine-rich repeat receptor protein kinase EMS1Add BLAST1174

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi171N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi187N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi208N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi259N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi414N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi435N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi555N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi629N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi682N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi711N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi746N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Modified residuei914PhosphothreonineBy similarity1
Modified residuei990PhosphotyrosineBy similarity1
Modified residuei1085PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylates in vitro.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9LYN8
PRIDEiQ9LYN8

Expressioni

Tissue specificityi

Present in young buds, open flowers and siliques but absent from mature leaves and roots. Strongly expressed in the young organ primordia, and as the anthers and ovules developed, became focused in the microsporangia and in the distal and chalazal regions of the ovule. In cv. Landsberg erecta, only expressed in the anthers of young floral buds.

Developmental stagei

Expressed during the differentiation of microsporocytes and tapetal cells. Also expressed in the meiocytes and young pollen grains until pollen mitosis II.2 Publications

Gene expression databases

ExpressionAtlasiQ9LYN8 baseline and differential
GenevisibleiQ9LYN8 AT

Interactioni

Subunit structurei

Interacts with TPD1.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi15898, 5 interactors
DIPiDIP-29701N
IntActiQ9LYN8, 3 interactors
STRINGi3702.AT5G07280.1

Structurei

3D structure databases

ProteinModelPortaliQ9LYN8
SMRiQ9LYN8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 87LRR 1Add BLAST24
Repeati90 – 112LRR 2Add BLAST23
Repeati114 – 137LRR 3Add BLAST24
Repeati138 – 160LRR 4Add BLAST23
Repeati163 – 185LRR 5Add BLAST23
Repeati187 – 209LRR 6Add BLAST23
Repeati235 – 257LRR 7Add BLAST23
Repeati259 – 281LRR 8Add BLAST23
Repeati283 – 304LRR 9Add BLAST22
Repeati330 – 352LRR 10Add BLAST23
Repeati354 – 376LRR 11Add BLAST23
Repeati378 – 400LRR 12Add BLAST23
Repeati402 – 425LRR 13Add BLAST24
Repeati426 – 447LRR 14Add BLAST22
Repeati449 – 471LRR 15Add BLAST23
Repeati473 – 496LRR 16Add BLAST24
Repeati497 – 520LRR 17Add BLAST24
Repeati521 – 543LRR 18Add BLAST23
Repeati545 – 567LRR 19Add BLAST23
Repeati581 – 603LRR 20Add BLAST23
Repeati605 – 628LRR 21Add BLAST24
Repeati629 – 651LRR 22Add BLAST23
Repeati653 – 675LRR 23Add BLAST23
Repeati677 – 697LRR 24Add BLAST21
Repeati701 – 723LRR 25Add BLAST23
Repeati725 – 748LRR 26Add BLAST24
Repeati749 – 772LRR 27Add BLAST24
Repeati773 – 795LRR 28Add BLAST23
Domaini917 – 1192Protein kinasePROSITE-ProRule annotationAdd BLAST276

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHKA Eukaryota
COG0515 LUCA
COG4886 LUCA
HOGENOMiHOG000116551
InParanoidiQ9LYN8
OMAiCSIPKAI
OrthoDBiEOG093600MT
PhylomeDBiQ9LYN8

Family and domain databases

Gene3Di3.80.10.10, 5 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR013210 LRR_N_plant-typ
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00560 LRR_1, 1 hit
PF13516 LRR_6, 2 hits
PF13855 LRR_8, 4 hits
PF08263 LRRNT_2, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 14 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LYN8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAFLTALFLF LFFSFSSSAI VDLSSETTSL ISFKRSLENP SLLSSWNVSS
60 70 80 90 100
SASHCDWVGV TCLLGRVNSL SLPSLSLRGQ IPKEISSLKN LRELCLAGNQ
110 120 130 140 150
FSGKIPPEIW NLKHLQTLDL SGNSLTGLLP RLLSELPQLL YLDLSDNHFS
160 170 180 190 200
GSLPPSFFIS LPALSSLDVS NNSLSGEIPP EIGKLSNLSN LYMGLNSFSG
210 220 230 240 250
QIPSEIGNIS LLKNFAAPSC FFNGPLPKEI SKLKHLAKLD LSYNPLKCSI
260 270 280 290 300
PKSFGELHNL SILNLVSAEL IGLIPPELGN CKSLKSLMLS FNSLSGPLPL
310 320 330 340 350
ELSEIPLLTF SAERNQLSGS LPSWMGKWKV LDSLLLANNR FSGEIPHEIE
360 370 380 390 400
DCPMLKHLSL ASNLLSGSIP RELCGSGSLE AIDLSGNLLS GTIEEVFDGC
410 420 430 440 450
SSLGELLLTN NQINGSIPED LWKLPLMALD LDSNNFTGEI PKSLWKSTNL
460 470 480 490 500
MEFTASYNRL EGYLPAEIGN AASLKRLVLS DNQLTGEIPR EIGKLTSLSV
510 520 530 540 550
LNLNANMFQG KIPVELGDCT SLTTLDLGSN NLQGQIPDKI TALAQLQCLV
560 570 580 590 600
LSYNNLSGSI PSKPSAYFHQ IEMPDLSFLQ HHGIFDLSYN RLSGPIPEEL
610 620 630 640 650
GECLVLVEIS LSNNHLSGEI PASLSRLTNL TILDLSGNAL TGSIPKEMGN
660 670 680 690 700
SLKLQGLNLA NNQLNGHIPE SFGLLGSLVK LNLTKNKLDG PVPASLGNLK
710 720 730 740 750
ELTHMDLSFN NLSGELSSEL STMEKLVGLY IEQNKFTGEI PSELGNLTQL
760 770 780 790 800
EYLDVSENLL SGEIPTKICG LPNLEFLNLA KNNLRGEVPS DGVCQDPSKA
810 820 830 840 850
LLSGNKELCG RVVGSDCKIE GTKLRSAWGI AGLMLGFTII VFVFVFSLRR
860 870 880 890 900
WAMTKRVKQR DDPERMEESR LKGFVDQNLY FLSGSRSREP LSINIAMFEQ
910 920 930 940 950
PLLKVRLGDI VEATDHFSKK NIIGDGGFGT VYKACLPGEK TVAVKKLSEA
960 970 980 990 1000
KTQGNREFMA EMETLGKVKH PNLVSLLGYC SFSEEKLLVY EYMVNGSLDH
1010 1020 1030 1040 1050
WLRNQTGMLE VLDWSKRLKI AVGAARGLAF LHHGFIPHII HRDIKASNIL
1060 1070 1080 1090 1100
LDGDFEPKVA DFGLARLISA CESHVSTVIA GTFGYIPPEY GQSARATTKG
1110 1120 1130 1140 1150
DVYSFGVILL ELVTGKEPTG PDFKESEGGN LVGWAIQKIN QGKAVDVIDP
1160 1170 1180 1190
LLVSVALKNS QLRLLQIAML CLAETPAKRP NMLDVLKALK EI
Length:1,192
Mass (Da):129,800
Last modified:October 1, 2000 - v1
Checksum:iD02C8B8FF6B09F7D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti131 – 132RL → SR in CAD42912 (PubMed:12401166).Curated2
Sequence conflicti138Q → E in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti155P → L in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti209I → T in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti258H → Q in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti273L → S in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti325M → I in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti347H → R in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti572E → D in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti852A → V in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti866M → I in CAD42912 (PubMed:12401166).Curated1
Sequence conflicti1075V → I in CAD42912 (PubMed:12401166).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496433 mRNA Translation: CAD42912.1
AJ488154 mRNA Translation: CAD32463.1
FJ708773 mRNA Translation: ACN59364.1
AL163652 Genomic DNA Translation: CAB87284.1
CP002688 Genomic DNA Translation: AED91132.1
PIRiT48499
RefSeqiNP_196345.1, NM_120810.3
UniGeneiAt.32742

Genome annotation databases

EnsemblPlantsiAT5G07280.1; AT5G07280.1; AT5G07280
GeneIDi830619
GrameneiAT5G07280.1; AT5G07280.1; AT5G07280
KEGGiath:AT5G07280

Similar proteinsi

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ496433 mRNA Translation: CAD42912.1
AJ488154 mRNA Translation: CAD32463.1
FJ708773 mRNA Translation: ACN59364.1
AL163652 Genomic DNA Translation: CAB87284.1
CP002688 Genomic DNA Translation: AED91132.1
PIRiT48499
RefSeqiNP_196345.1, NM_120810.3
UniGeneiAt.32742

3D structure databases

ProteinModelPortaliQ9LYN8
SMRiQ9LYN8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15898, 5 interactors
DIPiDIP-29701N
IntActiQ9LYN8, 3 interactors
STRINGi3702.AT5G07280.1

Proteomic databases

PaxDbiQ9LYN8
PRIDEiQ9LYN8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G07280.1; AT5G07280.1; AT5G07280
GeneIDi830619
GrameneiAT5G07280.1; AT5G07280.1; AT5G07280
KEGGiath:AT5G07280

Organism-specific databases

AraportiAT5G07280
TAIRilocus:2182870 AT5G07280

Phylogenomic databases

eggNOGiENOG410IHKA Eukaryota
COG0515 LUCA
COG4886 LUCA
HOGENOMiHOG000116551
InParanoidiQ9LYN8
OMAiCSIPKAI
OrthoDBiEOG093600MT
PhylomeDBiQ9LYN8

Miscellaneous databases

PROiPR:Q9LYN8

Gene expression databases

ExpressionAtlasiQ9LYN8 baseline and differential
GenevisibleiQ9LYN8 AT

Family and domain databases

Gene3Di3.80.10.10, 5 hits
InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR013210 LRR_N_plant-typ
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00560 LRR_1, 1 hit
PF13516 LRR_6, 2 hits
PF13855 LRR_8, 4 hits
PF08263 LRRNT_2, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 14 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEMS1_ARATH
AccessioniPrimary (citable) accession number: Q9LYN8
Secondary accession number(s): C0LGS9, Q8GSM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: October 1, 2000
Last modified: October 10, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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