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Protein

Calcium-transporting ATPase 12, plasma membrane-type

Gene

ACA12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol out of the cell or into organelles.By similarity

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Activity regulationi

Activated by calmodulin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4534-aspartylphosphate intermediateBy similarity1
Metal bindingi751MagnesiumBy similarity1
Metal bindingi755MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • calmodulin binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G63380-MONOMER
ReactomeiR-ATH-418359 Reduction of cytosolic Ca++ levels
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.2.42 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 12, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 12
Gene namesi
Name:ACA12
Ordered Locus Names:At3g63380
ORF Names:MAA21_10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G63380
TAIRilocus:2087363 AT3G63380

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 152CytoplasmicSequence analysisAdd BLAST152
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Topological domaini174 – 191LumenalSequence analysisAdd BLAST18
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 341CytoplasmicSequence analysisAdd BLAST129
Transmembranei342 – 361HelicalSequence analysisAdd BLAST20
Topological domaini362 – 397LumenalSequence analysisAdd BLAST36
Transmembranei398 – 415HelicalSequence analysisAdd BLAST18
Topological domaini416 – 806CytoplasmicSequence analysisAdd BLAST391
Transmembranei807 – 825HelicalSequence analysisAdd BLAST19
Topological domaini826 – 836LumenalSequence analysisAdd BLAST11
Transmembranei837 – 857HelicalSequence analysisAdd BLAST21
Topological domaini858 – 877CytoplasmicSequence analysisAdd BLAST20
Transmembranei878 – 900HelicalSequence analysisAdd BLAST23
Topological domaini901 – 909LumenalSequence analysis9
Transmembranei910 – 930HelicalSequence analysisAdd BLAST21
Topological domaini931 – 948CytoplasmicSequence analysisAdd BLAST18
Transmembranei949 – 970HelicalSequence analysisAdd BLAST22
Topological domaini971 – 980LumenalSequence analysis10
Transmembranei981 – 1002HelicalSequence analysisAdd BLAST22
Topological domaini1003 – 1006CytoplasmicSequence analysis4

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464181 – 1033Calcium-transporting ATPase 12, plasma membrane-typeAdd BLAST1033

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei37PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9LY77
PRIDEiQ9LY77

Expressioni

Gene expression databases

ExpressionAtlasiQ9LY77 baseline and differential
GenevisibleiQ9LY77 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi10827, 19 interactors
IntActiQ9LY77, 16 interactors
STRINGi3702.AT3G63380.1

Structurei

3D structure databases

ProteinModelPortaliQ9LY77
SMRiQ9LY77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 36Interaction with calmodulinCuratedAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
HOGENOMiHOG000265623
InParanoidiQ9LY77
KOiK01537
OMAiQESIHED
OrthoDBiEOG093601Z7
PhylomeDBiQ9LY77

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9LY77-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRDLKEYDYS ALLLNLTTSS LNKAQRRWRF AYAAIYSMRA MLSLVKEIVP
60 70 80 90 100
ARIDPKTSDA SLSLSYTALE SGEGAKINSM PLSYVPAIDQ EQLVEIMKGK
110 120 130 140 150
DLPGIQALGG VEGVAASLRT NPTKGIHGNE QEVSRRRDLF GSNTYHKPPP
160 170 180 190 200
KGLLFFVYEA FKDLTILILL VCAIFSLGFG IKEHGIKEGW YEGGSIFVAV
210 220 230 240 250
FLVIVVSALS NFRQERQFDK LSKISNNIKV EVLRDSRRQH ISIFDVVVGD
260 270 280 290 300
VVFLKIGDQI PADGLFLEGH SLQVDESSMT GESDHLEVDH KDNPFLFSGT
310 320 330 340 350
KIVDGFAQML VVSVGMSTTW GQTMSSINQD SSERTPLQVR LDTLTSTIGK
360 370 380 390 400
IGLTVAALVL VVLLVRYFTG NTEKEGKREY NGSKTPVDTV VNSVVRIVAA
410 420 430 440 450
AVTIVVVAIP EGLPLAVTLT LAYSMKRMMS DQAMVRKLSA CETMGSATVI
460 470 480 490 500
CTDKTGTLTL NEMKVTKFWL GQESIHEDST KMISPDVLDL LYQGTGLNTT
510 520 530 540 550
GSVCVSDSGS TPEFSGSPTE KALLSWTVLN LGMDMESVKQ KHEVLRVETF
560 570 580 590 600
SSAKKRSGVL VRRKSDNTVH VHWKGAAEMV LAMCSHYYTS TGSVDLMDST
610 620 630 640 650
AKSRIQAIIQ GMAASSLRCI AFAHKIASND SVLEEDGLTL MGIVGLKDPC
660 670 680 690 700
RPGVSKAVET CKLAGVTIKM ITGDNVFTAK AIAFECGILD HNDKDEEDAV
710 720 730 740 750
VEGVQFRNYT DEERMQKVDK IRVMARSSPS DKLLMVKCLR LKGHVVAVTG
760 770 780 790 800
DGTNDAPALK EADIGLSMGI QGTEVAKESS DIVILDDNFA SVATVLKWGR
810 820 830 840 850
CVYNNIQKFI QFQLTVNVAA LVINFIAAIS AGEVPLTAVQ LLWVNLIMDT
860 870 880 890 900
LGALALATER PTNELLKRKP VGRTEALITN VMWRNLLVQS LYQIAVLLIL
910 920 930 940 950
QFKGMSIFSV RKEVKDTLIF NTFVLCQVFN EFNAREMEKK NVFKGLHRNR
960 970 980 990 1000
LFIGIIAITI VLQVIMVEFL KKFADTVRLN GWQWGTCIAL ASLSWPIGFF
1010 1020 1030
TKFIPVSETP FLSYFKNPRS LFKGSRSPSL KKP
Length:1,033
Mass (Da):113,707
Last modified:October 1, 2000 - v1
Checksum:iD0898F370970D451
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti239Q → R in BAC41935 (PubMed:11910074).Curated1
Sequence conflicti239Q → R in AAO64912 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163818 Genomic DNA Translation: CAB87791.1
CP002686 Genomic DNA Translation: AEE80473.1
AK117260 mRNA Translation: BAC41935.1
BT005977 mRNA Translation: AAO64912.1
PIRiT49179
RefSeqiNP_191897.1, NM_116203.3
UniGeneiAt.26192
At.33936

Genome annotation databases

EnsemblPlantsiAT3G63380.1; AT3G63380.1; AT3G63380
GeneIDi825513
GrameneiAT3G63380.1; AT3G63380.1; AT3G63380
KEGGiath:AT3G63380

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163818 Genomic DNA Translation: CAB87791.1
CP002686 Genomic DNA Translation: AEE80473.1
AK117260 mRNA Translation: BAC41935.1
BT005977 mRNA Translation: AAO64912.1
PIRiT49179
RefSeqiNP_191897.1, NM_116203.3
UniGeneiAt.26192
At.33936

3D structure databases

ProteinModelPortaliQ9LY77
SMRiQ9LY77
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10827, 19 interactors
IntActiQ9LY77, 16 interactors
STRINGi3702.AT3G63380.1

Protein family/group databases

TCDBi3.A.3.2.42 the p-type atpase (p-atpase) superfamily

Proteomic databases

PaxDbiQ9LY77
PRIDEiQ9LY77

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G63380.1; AT3G63380.1; AT3G63380
GeneIDi825513
GrameneiAT3G63380.1; AT3G63380.1; AT3G63380
KEGGiath:AT3G63380

Organism-specific databases

AraportiAT3G63380
TAIRilocus:2087363 AT3G63380

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
HOGENOMiHOG000265623
InParanoidiQ9LY77
KOiK01537
OMAiQESIHED
OrthoDBiEOG093601Z7
PhylomeDBiQ9LY77

Enzyme and pathway databases

BioCyciARA:AT3G63380-MONOMER
ReactomeiR-ATH-418359 Reduction of cytosolic Ca++ levels
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Miscellaneous databases

PROiPR:Q9LY77

Gene expression databases

ExpressionAtlasiQ9LY77 baseline and differential
GenevisibleiQ9LY77 AT

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACA12_ARATH
AccessioniPrimary (citable) accession number: Q9LY77
Secondary accession number(s): Q8GZ18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: September 12, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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