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UniProtKB - Q9LY71 (CKX6_ARATH)
Protein
Cytokinin dehydrogenase 6
Gene
CKX6
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the oxidation of cytokinins, a family of N6-substituted adenine derivatives that are plant hormones, where the substituent is an isopentenyl group.
By similarity1 PublicationCatalytic activityi
- EC:1.5.99.12
Cofactori
FADBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 114 | FAD; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 172 | FAD; via amide nitrogenBy similarity | 1 | |
Binding sitei | 177 | FADBy similarity | 1 | |
Binding sitei | 238 | FAD; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 485 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 104 – 108 | FADBy similarity | 5 | |
Nucleotide bindingi | 109 – 110 | FADBy similarity | 2 | |
Nucleotide bindingi | 183 – 187 | FADBy similarity | 5 | |
Nucleotide bindingi | 520 – 523 | FADBy similarity | 4 |
GO - Molecular functioni
- cytokinin dehydrogenase activity Source: TAIR
- FAD binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- cytokinin metabolic process Source: InterPro
- stomatal complex morphogenesis Source: TAIR
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein |
Names & Taxonomyi
Protein namesi | Recommended name: Cytokinin dehydrogenase 6 (EC:1.5.99.12)Alternative name(s): Cytokinin oxidase 6 Short name: AtCKX6 Short name: AtCKX7 Short name: CKO6 |
Gene namesi | Name:CKX6 Synonyms:CKX7 Ordered Locus Names:At3g63440 ORF Names:MAA21_70 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G63440 |
TAIRi | locus:2087423, AT3G63440 |
Subcellular locationi
Extracellular region or secreted
- extracellular space By similarity
Extracellular region or secreted
- extracellular space Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | Sequence analysisAdd BLAST | 29 | |
ChainiPRO_0000020423 | 30 – 533 | Cytokinin dehydrogenase 6Add BLAST | 504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 109 | Pros-8alpha-FAD histidineBy similarity | 1 | |
Glycosylationi | 215 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 290 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 424 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 430 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | Q9LY71 |
Expressioni
Tissue specificityi
Expressed in the vascular system of roots, of young growing leaves and of the most apical portion of the growing stem. No expression in the root apical meristem. In flowers, restricted to the vascular bundles and transmitting tissue of developing gynoeciae.1 Publication
Developmental stagei
Expressed during the maturation phase of stomatal guard cells but not in fully mature stomata. In the root vasculature, detected soon after germination, with a maxima around the lateral root primordia.1 Publication
Gene expression databases
ExpressionAtlasi | Q9LY71, baseline and differential |
Genevisiblei | Q9LY71, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 68 – 248 | FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST | 181 |
Sequence similaritiesi
Belongs to the oxygen-dependent FAD-linked oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q9LY71 |
OMAi | FERRKHT |
OrthoDBi | 733611at2759 |
PhylomeDBi | Q9LY71 |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9LY71-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYLHASLLR KRTMLIVRSF TILLLSCIAF KLACCFSSSI SSLKALPLVG
60 70 80 90 100
HLEFEHVHHA SKDFGNRYQL IPLAVLHPKS VSDIASTIRH IWMMGTHSQL
110 120 130 140 150
TVAARGRGHS LQGQAQTRHG IVIHMESLHP QKLQVYSVDS PAPYVDVSGG
160 170 180 190 200
ELWINILHET LKYGLAPKSW TDYLHLTVGG TLSNAGISGQ AFRHGPQISN
210 220 230 240 250
VHQLEIVTGK GEILNCTKRQ NSDLFNGVLG GLGQFGIITR ARIALEPAPT
260 270 280 290 300
MVKWIRVLYL DFAAFAKDQE QLISAQGHKF DYIEGFVIIN RTGLLNSWRL
310 320 330 340 350
SFTAEEPLEA SQFKFDGRTL YCLELAKYLK QDNKDVINQE VKETLSELSY
360 370 380 390 400
VTSTLFTTEV AYEAFLDRVH VSEVKLRSKG QWEVPHPWLN LLVPRSKINE
410 420 430 440 450
FARGVFGNIL TDTSNGPVIV YPVNKSKWDN QTSAVTPEEE VFYLVAILTS
460 470 480 490 500
ASPGSAGKDG VEEILRRNRR ILEFSEEAGI GLKQYLPHYT TREEWRSHFG
510 520 530
DKWGEFVRRK SRYDPLAILA PGHRIFQKAV SYS
Sequence cautioni
The sequence BAF00384 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAB87797 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL163818 Genomic DNA Translation: CAB87797.1 Sequence problems. CP002686 Genomic DNA Translation: AEE80482.1 AK228457 mRNA Translation: BAF00384.1 Different initiation. |
PIRi | T49185 |
RefSeqi | NP_191903.3, NM_116209.3 |
Genome annotation databases
EnsemblPlantsi | AT3G63440.1; AT3G63440.1; AT3G63440 |
GeneIDi | 825519 |
Gramenei | AT3G63440.1; AT3G63440.1; AT3G63440 |
KEGGi | ath:AT3G63440 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL163818 Genomic DNA Translation: CAB87797.1 Sequence problems. CP002686 Genomic DNA Translation: AEE80482.1 AK228457 mRNA Translation: BAF00384.1 Different initiation. |
PIRi | T49185 |
RefSeqi | NP_191903.3, NM_116209.3 |
3D structure databases
SMRi | Q9LY71 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT3G63440.1 |
Proteomic databases
PaxDbi | Q9LY71 |
Genome annotation databases
EnsemblPlantsi | AT3G63440.1; AT3G63440.1; AT3G63440 |
GeneIDi | 825519 |
Gramenei | AT3G63440.1; AT3G63440.1; AT3G63440 |
KEGGi | ath:AT3G63440 |
Organism-specific databases
Araporti | AT3G63440 |
TAIRi | locus:2087423, AT3G63440 |
Phylogenomic databases
eggNOGi | KOG1231, Eukaryota |
HOGENOMi | CLU_024955_1_0_1 |
InParanoidi | Q9LY71 |
OMAi | FERRKHT |
OrthoDBi | 733611at2759 |
PhylomeDBi | Q9LY71 |
Miscellaneous databases
PROi | PR:Q9LY71 |
Gene expression databases
ExpressionAtlasi | Q9LY71, baseline and differential |
Genevisiblei | Q9LY71, AT |
Family and domain databases
Gene3Di | 3.30.43.10, 1 hit 3.30.465.10, 1 hit 3.40.462.10, 1 hit |
InterProi | View protein in InterPro IPR016170, Cytok_DH_C_sf IPR015345, Cytokinin_DH_FAD/cytokin-bd IPR016166, FAD-bd_PCMH IPR036318, FAD-bd_PCMH-like_sf IPR016167, FAD-bd_PCMH_sub1 IPR016169, FAD-bd_PCMH_sub2 IPR016164, FAD-linked_Oxase-like_C IPR006094, Oxid_FAD_bind_N |
Pfami | View protein in Pfam PF09265, Cytokin-bind, 1 hit PF01565, FAD_binding_4, 1 hit |
SUPFAMi | SSF55103, SSF55103, 1 hit SSF56176, SSF56176, 1 hit |
PROSITEi | View protein in PROSITE PS51387, FAD_PCMH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CKX6_ARATH | |
Accessioni | Q9LY71Primary (citable) accession number: Q9LY71 Secondary accession number(s): Q0WR65 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 24, 2005 |
Last sequence update: | February 8, 2011 | |
Last modified: | February 23, 2022 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families