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Protein

Probable pectinesterase 49

Gene

PME49

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase PPME1 (PPME1), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Putative pectinesterase 11 (PME11), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase 67 (PME67), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 49 (PME49), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Probable pectinesterase 50 (PME50), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Pectinesterase QRT1 (QRT1), Putative pectinesterase 63 (PME63), Probable pectinesterase 30 (PME30), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Pectinesterase/pectinesterase inhibitor 18 (PME18), Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 48 (PME48), Pectinesterase 4 (PME4), Putative pectinesterase 14 (PME14), Probable pectinesterase 55 (PME55), Probable pectinesterase 56 (PME56), Probable pectinesterase 66 (PME66), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase 8 (PME8), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase/pectinesterase inhibitor 3 (PME3), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Pectinesterase 1 (PME1), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Putative pectinesterase 57 (PME57), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase 5 (PME5), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 29 (PME29), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Uncharacterized protein (AXX17_At3g05180), Pectinesterase (AXX17_At5g52320), Pectinesterase (AXX17_At3g17940), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At5g50300), PME26 (AXX17_At3g14890), Pectinesterase (AXX17_At4g02940), Pectinesterase (At4g15980), Pectinesterase (At3g14310), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (At5g26810), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g45340), Pectinesterase (At3g10720), Pectinesterase (AXX17_At5g04430), Pectinesterase (AXX17_At1g11890), Uncharacterized protein (AXX17_At5g27870), Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At5g50310), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At3g06710), Pectinesterase (AXX17_At3g55110), Pectinesterase (At5g20860), Pectinesterase (AXX17_At4g38040), Pectinesterase (AXX17_At1g04730), Pectinesterase (AXX17_At1g11900), Pectinesterase (AXX17_At2g33410), Pectinesterase, Pectinesterase (AXX17_At2g22270), Pectinesterase (AXX17_At2g40510), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g18720), Pectinesterase (AXX17_At4g02930), Pectinesterase (AXX17_At5g47900), Pectinesterase (AXX17_At4g38050), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At1g11920), Pectinesterase (At3g49220), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g19650), Pectinesterase, Pectinesterase (AXX17_At3g05190), Pectinesterase (AXX17_At3g30550), Pectinesterase (AXX17_At4g06570), Pectinesterase (At1g53840), Pectinesterase, Pectinesterase (AXX17_At4g02900), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At5g45990), Pectinesterase (AXX17_At2g44790), Pectinesterase (F14I3.7), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At3g26070), Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At3g10570), Pectinesterase (AXX17_At3g43380), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (PME44), Pectinesterase (PMEPCRF), Pectinesterase (AXX17_At1g48290), Pectinesterase, Pectinesterase (AXX17_At2g22280)
  2. Probable pectate lyase 10 (At3g24670), Putative pectate lyase 11 (At3g27400), Probable pectate lyase 15 (At4g13710), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 1 (At1g04680), Putative pectate lyase 14 (At4g13210), Probable pectate lyase 19 (At5g15110), Putative pectate lyase 21 (At5g55720), Probable pectate lyase 22 (At5g63180), Putative pectate lyase 2 (At1g11920), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 6 (At2g02720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Probable pectate lyase 13 (PMR6), Probable pectate lyase 12 (At3g53190), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 9 (At3g24230), Pectate lyase (At3g01270), Pectate lyase (At3g09540), Pectate lyase (AXX17_At3g09180), Pectate lyase (At5g09280), Pectate lyase (At5g04310), Pectate lyase (F11F8_12), Pectate lyase (At1g14420), Pectate lyase (At3g55140), Pectate lyase (AXX17_At4g28670), Pectate lyase, Pectate lyase (At3g27400), Pectate lyase (AXX17_At5g03700), Pectate lyase (AXX17_At4g25610), Pectate lyase (AXX17_At3g00310), Pectate lyase (AXX17_At3g49720), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g06910), Pectate lyase (AXX17_At3g47590), Pectate lyase (At5g04310), Pectate lyase (At4g13710), Pectate lyase, Pectate lyase (At3g53190), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At5g08800), Pectate lyase (At4g13210), Pectate lyase (At3g55140), Pectate lyase (At3g01270), Pectate lyase (AXX17_At4g15660), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At2g01680), Pectate lyase (AXX17_At3g26720), Pectate lyase (AXX17_At5g14600), Pectate lyase (AXX17_At1g04020), Pectate lyase (AXX17_At1g12300), Pectate lyase (At3g07010), Pectate lyase (AXX17_At1g15010), Pectate lyase (AXX17_At1g30780), Pectate lyase (AXX17_At5g47570), Pectate lyase, Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At1g61710), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g26200), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g62760), Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei174SubstrateBy similarity1
Sitei196Transition state stabilizerBy similarity1
Active sitei197Proton donorBy similarity1
Active sitei218NucleophileBy similarity1
Binding sitei275SubstrateBy similarity1
Binding sitei277SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT5G07420-MONOMER
BRENDAi3.1.1.11 399
UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase 49 (EC:3.1.1.11)
Short name:
PE 49
Alternative name(s):
Pectin methylesterase 49
Short name:
AtPME49
Gene namesi
Name:PME49
Synonyms:ARATH49
Ordered Locus Names:At5g07420
ORF Names:T2I1.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G07420
TAIRilocus:2183349 AT5G07420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000037169723 – 361Probable pectinesterase 49Add BLAST339

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi128N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9LY18
PRIDEiQ9LY18

Expressioni

Tissue specificityi

Expressed in flower buds.1 Publication

Gene expression databases

ExpressionAtlasiQ9LY18 baseline and differential
GenevisibleiQ9LY18 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G07420.1

Structurei

3D structure databases

ProteinModelPortaliQ9LY18
SMRiQ9LY18
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9LY18
OMAiIVWATNF
OrthoDBiEOG09360CIN
PhylomeDBiQ9LY18

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR000070 Pectinesterase_cat
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
SUPFAMiSSF51126 SSF51126, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9LY18-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGYISLALVA LLVFFASPVV LADDITPIPA DRAQIPQWFM ANVKPFSQRR
60 70 80 90 100
GTLDPELEAA EASRRVIIVN QNGGGDFKTI NAAIKSIPLA NKNRVIIKLA
110 120 130 140 150
PGIYHEKVTV DVGRPYVTLL GKPGAETNLT YAGTAAKYGT VESATLIVWA
160 170 180 190 200
TNFLAANLNI INTSPMPKPG TQGQALAMRI NGDKAAFYNC RFYGFQDTLC
210 220 230 240 250
DDRGNHFFKN CYIEGTYDFI FGRGASLYLT TQLHAVGDGL RVIAAHNRQS
260 270 280 290 300
TTEQNGYSFV HCKVTGVGTG IYLGRAWMSH PKVVYSYTEM SSVVNPSGWQ
310 320 330 340 350
ENRVRAHDKT VFYGEYMCTG PGSHKAKRVA HTQDIDNKEA SQFLTLGYIK
360
GSKWLLPPPA Y
Length:361
Mass (Da):39,658
Last modified:October 1, 2000 - v1
Checksum:iC352BE09718E186B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163912 Genomic DNA Translation: CAB87931.1
CP002688 Genomic DNA Translation: AED91155.1
BT002768 mRNA Translation: AAO22596.1
BT005059 mRNA Translation: AAO50592.1
AY086762 mRNA Translation: AAM63813.1
PIRiT49881
RefSeqiNP_196359.1, NM_120824.4
UniGeneiAt.32723

Genome annotation databases

EnsemblPlantsiAT5G07420.1; AT5G07420.1; AT5G07420
GeneIDi830633
GrameneiAT5G07420.1; AT5G07420.1; AT5G07420
KEGGiath:AT5G07420

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163912 Genomic DNA Translation: CAB87931.1
CP002688 Genomic DNA Translation: AED91155.1
BT002768 mRNA Translation: AAO22596.1
BT005059 mRNA Translation: AAO50592.1
AY086762 mRNA Translation: AAM63813.1
PIRiT49881
RefSeqiNP_196359.1, NM_120824.4
UniGeneiAt.32723

3D structure databases

ProteinModelPortaliQ9LY18
SMRiQ9LY18
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G07420.1

Proteomic databases

PaxDbiQ9LY18
PRIDEiQ9LY18

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G07420.1; AT5G07420.1; AT5G07420
GeneIDi830633
GrameneiAT5G07420.1; AT5G07420.1; AT5G07420
KEGGiath:AT5G07420

Organism-specific databases

AraportiAT5G07420
TAIRilocus:2183349 AT5G07420

Phylogenomic databases

eggNOGiCOG4677 LUCA
HOGENOMiHOG000217409
InParanoidiQ9LY18
OMAiIVWATNF
OrthoDBiEOG09360CIN
PhylomeDBiQ9LY18

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

BioCyciARA:AT5G07420-MONOMER
BRENDAi3.1.1.11 399

Miscellaneous databases

PROiPR:Q9LY18

Gene expression databases

ExpressionAtlasiQ9LY18 baseline and differential
GenevisibleiQ9LY18 AT

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR000070 Pectinesterase_cat
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
SUPFAMiSSF51126 SSF51126, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPME49_ARATH
AccessioniPrimary (citable) accession number: Q9LY18
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: October 1, 2000
Last modified: October 10, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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