UniProtKB - Q9LW31 (FUS3_ARATH)
Protein
B3 domain-containing transcription factor FUS3
Gene
FUS3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Transcription regulator involved in gene regulation during late embryogenesis. Its expression to the epidermis is sufficient to control foliar organ identity by regulating positively the synthesis abscisic acid (ABA) and negatively gibberellin production. Negatively regulates TTG1 in the embryo. Positively regulates the abundance of the ABI3 protein in the seed. Cooperates with KIN10 to regulate developmental phase transitions and lateral organ development and act both as positive regulators of abscisic acid (ABA) signaling during germination (PubMed:22026387, PubMed:22902692).4 Publications
Activity regulationi
Phosphorylation by KIN10 is required to positively regulates embryogenesis, seed yield, and plant growth at high temperature.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 92 – 194 | TF-B3PROSITE-ProRule annotationAdd BLAST | 103 |
GO - Molecular functioni
- DNA binding Source: TAIR
- DNA-binding transcription factor activity Source: TAIR
- kinase binding Source: UniProtKB
GO - Biological processi
- embryo development ending in seed dormancy Source: TAIR
- negative regulation of gibberellin biosynthetic process Source: UniProtKB
- plant organ development Source: UniProtKB
- positive regulation of abscisic acid-activated signaling pathway Source: UniProtKB
- positive regulation of abscisic acid biosynthetic process Source: TAIR
- positive regulation of cell population proliferation Source: UniProtKB
- regulation of embryonic development Source: UniProtKB
- response to auxin Source: TAIR
- somatic embryogenesis Source: TAIR
- vegetative to reproductive phase transition of meristem Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: B3 domain-containing transcription factor FUS3Alternative name(s): Protein FUSCA3 |
Gene namesi | Name:FUS3 Ordered Locus Names:At3g26790 ORF Names:MDJ14.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G26790 |
TAIRi | locus:2088439, AT3G26790 |
Subcellular locationi
Nucleus
- Nucleus Curated
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Accumulation of anthocyanins in embryo. Presence of trichomes on cotyledons. Unusual pattern of storage product accumulation in embryos and cotyledons.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 55 | S → A: Increased seed abortion and delayed embryo development; when associated with A-56 and A-57. 1 Publication | 1 | |
Mutagenesisi | 55 | S → D: Delay in flowering; when associated with D-56 and D-57. 1 Publication | 1 | |
Mutagenesisi | 56 | S → A: Increased seed abortion and delayed embryo development; when associated with A-55 and A-57. 1 Publication | 1 | |
Mutagenesisi | 56 | S → D: Delay in flowering; when associated with D-55 and D-57. 1 Publication | 1 | |
Mutagenesisi | 57 | S → A: Increased seed abortion and delayed embryo development; when associated with A-55 and A-56. 1 Publication | 1 | |
Mutagenesisi | 57 | S → D: Delay in flowering; when associated with D-55 and D-56. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000375089 | 1 – 313 | B3 domain-containing transcription factor FUS3Add BLAST | 313 |
Post-translational modificationi
Phosphorylation by KIN10 increases its stability. Phosphorylated at one or more of the Ser-55, Ser-56 and/or Ser-57 residues.1 Publication
Proteomic databases
PaxDbi | Q9LW31 |
PRIDEi | Q9LW31 |
ProteomicsDBi | 230553 |
Expressioni
Tissue specificityi
Expressed in cotyledons and hypocotyls.1 Publication
Developmental stagei
Expressed in developing embryo. At globular stage, expressed in all cells of the embryo proper. At heart stage, preferentially expressed in the protodermal tissue. In mature embryo, expressed in the provascular tissue, root cap and mature epidermis. Expressed in the aleurone layer in mature seed. Expressed in leaf primordia and shoot apical meristem (PubMed:22026387).3 Publications
Gene expression databases
ExpressionAtlasi | Q9LW31, baseline and differential |
Genevisiblei | Q9LW31, AT |
Interactioni
Subunit structurei
Interacts with KIN10.
1 PublicationGO - Molecular functioni
- kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 7623, 2 interactors |
STRINGi | 3702.AT3G26790.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9LW31 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 233 – 245 | Poly-AsnAdd BLAST | 13 |
Phylogenomic databases
eggNOGi | ENOG502QPW6, Eukaryota |
HOGENOMi | CLU_084617_0_0_1 |
InParanoidi | Q9LW31 |
OMAi | IPIRMED |
OrthoDBi | 317013at2759 |
PhylomeDBi | Q9LW31 |
Family and domain databases
CDDi | cd10017, B3_DNA, 1 hit |
Gene3Di | 2.40.330.10, 1 hit |
InterProi | View protein in InterPro IPR003340, B3_DNA-bd IPR015300, DNA-bd_pseudobarrel_sf |
Pfami | View protein in Pfam PF02362, B3, 1 hit |
SMARTi | View protein in SMART SM01019, B3, 1 hit |
SUPFAMi | SSF101936, SSF101936, 1 hit |
PROSITEi | View protein in PROSITE PS50863, B3, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9LW31-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMVDENVETK ASTLVASVDH GFGSGSGHDH HGLSASVPLL GVNWKKRRMP
60 70 80 90 100
RQRRSSSSFN LLSFPPPMPP ISHVPTPLPA RKIDPRKLRF LFQKELKNSD
110 120 130 140 150
VSSLRRMILP KKAAEAHLPA LECKEGIPIR MEDLDGFHVW TFKYRYWPNN
160 170 180 190 200
NSRMYVLENT GDFVNAHGLQ LGDFIMVYQD LYSNNYVIQA RKASEEEEVD
210 220 230 240 250
VINLEEDDVY TNLTRIENTV VNDLLLQDFN HHNNNNNNNS NSNSNKCSYY
260 270 280 290 300
YPVIDDVTTN TESFVYDTTA LTSNDTPLDF LGGHTTTTNN YYSKFGTFDG
310
LGSVENISLD DFY
Sequence cautioni
The sequence AAC35246 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAC35247 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB01226 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 10 | K → N in AAC35246 (PubMed:9807814).Curated | 1 | |
Sequence conflicti | 75 | P → T in AAC35246 (PubMed:9807814).Curated | 1 | |
Sequence conflicti | 217 | E → G in AAC35246 (PubMed:9807814).Curated | 1 | |
Sequence conflicti | 239 – 241 | Missing in CAG25870 (PubMed:15208423).Curated | 3 | |
Sequence conflicti | 239 – 241 | Missing in AAS79559 (PubMed:15208423).Curated | 3 | |
Sequence conflicti | 244 – 245 | Missing in AAC35246 (PubMed:9807814).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF016264 Genomic DNA Translation: AAC35246.1 Different initiation. AF016265 mRNA Translation: AAC35247.1 Different initiation. AB016889 Genomic DNA Translation: BAB01226.1 Sequence problems. CP002686 Genomic DNA Translation: AEE77216.1 AJ630497 mRNA Translation: CAG25870.1 AY568669 mRNA Translation: AAS79559.1 |
RefSeqi | NP_566799.1, NM_113591.4 |
Genome annotation databases
EnsemblPlantsi | AT3G26790.1; AT3G26790.1; AT3G26790 |
GeneIDi | 822293 |
Gramenei | AT3G26790.1; AT3G26790.1; AT3G26790 |
KEGGi | ath:AT3G26790 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF016264 Genomic DNA Translation: AAC35246.1 Different initiation. AF016265 mRNA Translation: AAC35247.1 Different initiation. AB016889 Genomic DNA Translation: BAB01226.1 Sequence problems. CP002686 Genomic DNA Translation: AEE77216.1 AJ630497 mRNA Translation: CAG25870.1 AY568669 mRNA Translation: AAS79559.1 |
RefSeqi | NP_566799.1, NM_113591.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6J9B | X-ray | 1.90 | A/D | 89-201 | [»] | |
SMRi | Q9LW31 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 7623, 2 interactors |
STRINGi | 3702.AT3G26790.1 |
Proteomic databases
PaxDbi | Q9LW31 |
PRIDEi | Q9LW31 |
ProteomicsDBi | 230553 |
Genome annotation databases
EnsemblPlantsi | AT3G26790.1; AT3G26790.1; AT3G26790 |
GeneIDi | 822293 |
Gramenei | AT3G26790.1; AT3G26790.1; AT3G26790 |
KEGGi | ath:AT3G26790 |
Organism-specific databases
Araporti | AT3G26790 |
TAIRi | locus:2088439, AT3G26790 |
Phylogenomic databases
eggNOGi | ENOG502QPW6, Eukaryota |
HOGENOMi | CLU_084617_0_0_1 |
InParanoidi | Q9LW31 |
OMAi | IPIRMED |
OrthoDBi | 317013at2759 |
PhylomeDBi | Q9LW31 |
Miscellaneous databases
PROi | PR:Q9LW31 |
Gene expression databases
ExpressionAtlasi | Q9LW31, baseline and differential |
Genevisiblei | Q9LW31, AT |
Family and domain databases
CDDi | cd10017, B3_DNA, 1 hit |
Gene3Di | 2.40.330.10, 1 hit |
InterProi | View protein in InterPro IPR003340, B3_DNA-bd IPR015300, DNA-bd_pseudobarrel_sf |
Pfami | View protein in Pfam PF02362, B3, 1 hit |
SMARTi | View protein in SMART SM01019, B3, 1 hit |
SUPFAMi | SSF101936, SSF101936, 1 hit |
PROSITEi | View protein in PROSITE PS50863, B3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FUS3_ARATH | |
Accessioni | Q9LW31Primary (citable) accession number: Q9LW31 Secondary accession number(s): O82411, Q700C8, Q9SAT8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 26, 2009 |
Last sequence update: | May 26, 2009 | |
Last modified: | April 7, 2021 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references